Jurkat Cell Count Done
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cell count jurkat 1ต่อ5 24 hrs.
R+ 100000
C P H 1000 800 600 400 200 100 50 1ต่อ5
R+ 50000
48 hrs. C P H 1000 800 600 400 200 100 50
1ต่อ5 72 hrs. C P H 1000 800 600 400 200 100 50
R+ 25000
1 0.75 live 24 143 115 0 53 129 103 107 107 121 114
1 0.75 live 48 236 197 0 146 136 196 170 202 244 210
2 % vi dead 0 0 51 0 0 0 0 0 0 0
100 100 0 100 100 100 100 100 100 100
live
1.5 dead
181 131
0 0
23 60 106
25 4 1
live
2 0.75 dead
198 176 1 59 78 82 174 110 147 171
0 0 27 8 0 0 0 0 0 0
%vi dead 0 0 54 0 0 0 0 0 0 0
100 100 0 100 100 100 100 100 100 100
1 1.5 live
dead
live
2 1.5 dead
59
0
0
1
48.8 62.4
0 0
130 94 0 2 3 10 40 43 86 102
0 0 8 0 1 4 1 0 0 0
%vi
ใส่สาร C P H 1000 800 600 400 200 100 50
24h 100 94.01 0 27.54 58.68 74.25 96.41 91.62 100.6 100
48h 100 92.73 0 16.92 40 48.46 66.15 80.77 83.08 103.08
72h 100 72.31 0 1.54 2.31 7.69 30.77 33.08 66.15 78.46
3 %vi live
1.5 dead
%vi
%vi all
ava total cell proli inhibition live 1 and 2 162 100 123 75.93 0 0 38 23.46 94.5 58.33 104.5 64.51 107 66.05 107 66.05 121 74.69 114 70.37
Hrs. 100 100 47.92 93.75 99.07
167 157 0 46 98 124 161 153 168 167
0 0 80 32 0 8 0 0 0 0
100 100 0 58.97 100 93.94 100 100 100 100
%vi
%vi all
live
3 1.5 dead
ava live
130 150 0 22 52 63 86 105 108 134
0 0 35 10 2 1 2 0 0 0
100 100 0 68.75 96.3 98.44 97.73 100 100 100
100 100 1.15 85.6 98.77 99.48 99.24 100 100 100
188 174.33 0.33 75.67 88.67 113.67 143.33 139 166.33 171.67
%vi
100 100 3.45 88.06 100 100 100 100 100 100
total cell proli inhibition
100
%vi total cell proli inhibition
90 80
100 100 0 100 75 71.43 97.56 100 100 100
100 72.31 0 1.54 2.31 7.69 30.77 33.08 66.15 78.46
70 60 50 40 30 20 10 0 Row 38
Row 39
Row 40
Row 41
Row 42
Row 43
Row 44
Row 45
Row 46
R 4
110 100
100
96.41
94.01 92.73
90
100.6 91.62 80.77
80 70
83.08
74.25
72.31
103.08 100
66.15
78.46
66.15
58.68
60
Column D 48.46
50
Column E Column F
40
40 30
27.54
20
16.92
10 0 C
P
0
1.54
2.31
H
1000
800
30.77
33.08
400
200
7.69 600
100
50
100
l proli inhibition
ow 3
3 167 157 0 46 98 124 161 153 168 167
90 80 70
100 94.01 0 27.54 58.68 74.25 96.41 91.62 100.6 100
60 50
Col
40 30 20 10 0 Row 6
Row 7
Row 8
Row 9
Row 10
Row 11
Row 12
Row 13
Row 14
Row 15
100
total cell proli inhibition 3 only 100 92.73 0.18 40.25 47.16 60.46 76.24 73.94 88.48 91.31
100 115.38 0 16.92 40 48.46 66.15 80.77 83.08 103.08
90 80 70 60 50 Column O
40 30 20 10 0 Row 22
Row 23
Row 24
Row 25
Row 26
Row 27
Row 28
Row 29
Row 30
Row 31
100 90 80 70 60 50
Colu
40 30 20 10 Row 44
Row 45
Row 46
Row 47
0 Row 6
Row 7
Row 8
Row 9
Row 10
Row 11
Row 12
Row 13
Row 14
Row 15
100.6 83.08
103.08 100
78.46
66.15 Column D Column E Column F
100
50
110 100 90 80 70 Column N
60 50
Colu
40 30 20 10
Row 12
Row 29
Row 12
Row 13
Row 14
0 Row 6
Row 15
Column O
Row 30
Row 31
Column N
Row 13
Row 14
Row 15
Row 7
Row 8
Row 9
Row 10
Row 11
Row 12
Row 13
Row 14
Row 15
Row 12
Column P
Row 13
Row 14
Row 15
R+ 100000
C P H 1000 800 600 400 200 100 50 1ต่อ5
R+ 50000
48 hrs. C P H 1000 800 600 400 200 100 50
1ต่อ5 72 hrs. C P H 1000 800 600 400 200 100 50
R+ 25000
1 0.75 live 24 143 115 0 53 129 103 107 107 121 114
1 0.75 live 48 236 197 0 146 136 196 170 202 244 210
2 % vi dead 0 0 51 0 0 0 0 0 0 0
100 100 0 100 100 100 100 100 100 100
live
1.5 dead
181 131
0 0
23 60 106
25 4 1
live
2 0.75 dead
198 176 1 59 78 82 174 110 147 171
0 0 27 8 0 0 0 0 0 0
%vi dead 0 0 54 0 0 0 0 0 0 0
100 100 0 100 100 100 100 100 100 100
1 1.5 live
dead
live
2 1.5 dead
59
0
0
1
48.8 62.4
0 0
130 94 0 2 3 10 40 43 86 102
0 0 8 0 1 4 1 0 0 0
%vi
ใส่สาร C P H 1000 800 600 400 200 100 50
24h 100 94.01 0 27.54 58.68 74.25 96.41 91.62 100.6 100
48h 100 92.73 0 16.92 40 48.46 66.15 80.77 83.08 103.08
72h 100 72.31 0 1.54 2.31 7.69 30.77 33.08 66.15 78.46
3 %vi live
1.5 dead
%vi
%vi all
ava total cell proli inhibition live 1 and 2 162 100 123 75.93 0 0 38 23.46 94.5 58.33 104.5 64.51 107 66.05 107 66.05 121 74.69 114 70.37
Hrs. 100 100 47.92 93.75 99.07
167 157 0 46 98 124 161 153 168 167
0 0 80 32 0 8 0 0 0 0
100 100 0 58.97 100 93.94 100 100 100 100
%vi
%vi all
live
3 1.5 dead
ava live
130 150 0 22 52 63 86 105 108 134
0 0 35 10 2 1 2 0 0 0
100 100 0 68.75 96.3 98.44 97.73 100 100 100
100 100 1.15 85.6 98.77 99.48 99.24 100 100 100
188 174.33 0.33 75.67 88.67 113.67 143.33 139 166.33 171.67
%vi
100 100 3.45 88.06 100 100 100 100 100 100
total cell proli inhibition
100
%vi total cell proli inhibition
90 80
100 100 0 100 75 71.43 97.56 100 100 100
100 72.31 0 1.54 2.31 7.69 30.77 33.08 66.15 78.46
70 60 50 40 30 20 10 0 Row 38
Row 39
Row 40
Row 41
Row 42
Row 43
Row 44
Row 45
Row 46
R 4
110 100
100
96.41
94.01 92.73
90
100.6 91.62 80.77
80 70
83.08
74.25
72.31
103.08 100
66.15
78.46
66.15
58.68
60
Column D 48.46
50
Column E Column F
40
40 30
27.54
20
16.92
10 0 C
P
0
1.54
2.31
H
1000
800
30.77
33.08
400
200
7.69 600
100
50
100
l proli inhibition
ow 3
3 167 157 0 46 98 124 161 153 168 167
90 80 70
100 94.01 0 27.54 58.68 74.25 96.41 91.62 100.6 100
60 50
Col
40 30 20 10 0 Row 6
Row 7
Row 8
Row 9
Row 10
Row 11
Row 12
Row 13
Row 14
Row 15
100
total cell proli inhibition 3 only 100 92.73 0.18 40.25 47.16 60.46 76.24 73.94 88.48 91.31
100 115.38 0 16.92 40 48.46 66.15 80.77 83.08 103.08
90 80 70 60 50 Column O
40 30 20 10 0 Row 22
Row 23
Row 24
Row 25
Row 26
Row 27
Row 28
Row 29
Row 30
Row 31
100 90 80 70 60 50
Colu
40 30 20 10 Row 44
Row 45
Row 46
Row 47
0 Row 6
Row 7
Row 8
Row 9
Row 10
Row 11
Row 12
Row 13
Row 14
Row 15
100.6 83.08
103.08 100
78.46
66.15 Column D Column E Column F
100
50
110 100 90 80 70 Column N
60 50
Colu
40 30 20 10
Row 12
Row 29
Row 12
Row 13
Row 14
0 Row 6
Row 15
Column O
Row 30
Row 31
Column N
Row 13
Row 14
Row 15
Row 7
Row 8
Row 9
Row 10
Row 11
Row 12
Row 13
Row 14
Row 15
Row 12
Column P
Row 13
Row 14
Row 15
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