History of 'Genetic Engineering'
- Genetic engineering is the popular term for the more accurate 'Recombinant DNA Technology'. - The actual introduction of recombinant DNA technology into real life applications can not be attributed to a single discovery, however, it is rather an accumulation of different theories and ideas over a long period of time. - Of course it returns back to1944 when scientists working in Rockefeller institute in USA showed that inheritance depends on nucleic acid as a carrier of genetic information and not protein as previously thought. Then, it was followed by the breakthrough of Watson and Crick in 1953 who identified the DNA structure as a double helix based on x-ray diffraction studies. In 1961, scientists start their trials to know the “rule” controlling information storage in the DNA which was solved later on by identification of the 'triplet codes' which means that each sequence of 3 nitrogenous bases in the DNA determines the formation of 1 amino acid and by 1966, the triplet codes of the existing 20 amino acids were established.
In 1970, -Thefirst
syntheticgene
wasproducedina'test tube' by
Khorana
andhisgroupby
joiningtogetheritsbasepairs.
- Hamilton Smith discovered a group of enzymes which exist naturally in microorganisms and are capable of DNA cleavage at sequence-specific sites. Microorganisms use these enzymes as a defence tool to attack any invading organism. It was that “precise specificity” of their cutting that gave them a great role in recombinant DNA technology with the ability to cut certain 'gene sequence' out of the whole DNA molecule.
In 1970, -
Temin
and
Baltimore
independently
discovered
the
'Reverse
Transcriptase Enzyme' which can copy genetic information from RNA to DNA in certain viruses whose nucleic material is RNA [not DNA] in a reverse way [hence, the name] to transcription process in which genetic information is usually transferred from DNA to RNA 'mRNA' which is then translated by ribosomes into proteins. - This enzyme allowed the formation of a complementary DNA copy [called cDNA] of genes encoded by RNA in the laboratory, which then allow the production of the final gene product 'protein'.
In 1972, - Success of the first “recombinant” DNA experiment by “joining” restriction enzyme fragments [from 2 different organisms] together to constitute a functional molecule. - Then, plasmids were introduced as carriers for the constructed DNA fragments 'genes'. Plasmids are capable of independent [of bacterial DNA] replication in their bacterial hosts allowing the production of a large quantity of the inserted DNA fragment after growth of their bacterial host in a suitable medium producing a 'clone of bacterial cells' containing the same 'gene' [in plasmid]. - The ligation of a specific gene into a plasmid vector represents the 'recombinant' work of the technique.
In
1973,
- Construction of the first plasmid by Stanley Cohen ' referred to as pSC101' [p for plasmid, SC for the name of the scientist introduced it] for use in recombinant DNA experiments.
Restriction Endonucleases - Exist naturally in bacterial cells which use them to protect themselves against any invasion by foreign DNA from different strain.
- They are called ‘restriction’ because their activity is 'restricted' to ‘foreign’ DNA & called ‘endo-’ because they cut ‘within’ the DNA molecule.
- The host DNA is not cleaved by its “own” enzyme because the DNA sites susceptible to be cut by the enzyme are protected by “Methylation” which is done by another enzyme which is also included in each bacterial cell. .
*An example: E: 1st
EcoRI enzyme:
initial of the genus, Escherichia.
Co: 1st
2 initials of the species, coli.
R: strain designation (if the enzyme is present in a special strain). I: roman numeral show the order of discovery of the enzyme in this particular strain.
- Some enzymes, although obtained from different organisms, still can cleave the same site such as [HindIII & HsuI] or recognise the same nucleotide sequence, but cut at 2 different sites within that sequence such as (XmaI & SmaI) and are [in both cases] called 'Isoschizomers'.
- Due to the complementary nature of the base pairing in DNA molecules, a restriction endonuclease enzyme
---G
will cut both strands of the DNA molecules.
---CTTAA
- There are 2 types of cut ends; either 'sticky' or 'blunt' according to the type of the enzyme. - The sticky 'cohesive' ends are so called because the
AATTC---
*Recognition [GATTC] and
G-sequence
*cutting site [between G & A] of EcoRI enzyme, producing 'sticky ends'.
can combine with any complementary sequence [from another DNA molecule] as that produced in a plasmid
- -CCC
GGG-
vector by its cutting with the same enzyme to allow
-GGG
CCC-
recombination of both molecules.
Blunt ends produced by SmaI enzyme, derived from Serratia marcescens
- Recognition sequences vary widely. - Many of them are called Palindromic; that is, the sequence on one strand reads the same in the same direction [from 5’ to 3’, or 3’ to 5’] on the complementary strand. e.g., 5'-GTATAC-3' on one strand is complementary to 3'-CATATG-5', which is read GTATAC [i.e: the same] from 5' to 3'. While, 5'-GTAATG-3' is not a palindromic DNA sequence as its complement is 3’-CATTAC-5’ which is read CATTAC from 5’ to 3’ which is different from the other strand.
Ligation 'Recombination' - The process of inserting a target DNA fragment into a foreign vector DNA is called Ligation and is done by using an enzyme called 'DNA ligase'. - There are several types of DNA ligase as T4 DNA ligase which can link DNA molecules even those with blunt ends, however, such 'blunt-ended ligation' is less stable than 'sticky-ended ligation' as the later can occur naturally by hydrogen bonding between complementary bases 'annealing' before stabilization with the DNA ligase which catalyses bonds between ends of the DNA molecules.
References: * Books: - Alan E.H. Emery and Sue Malcolm (1995) An Introduction to Recombinant DNA in Medicine, 2nd edition. Wiley : Chichester, England.