Fleming

COURSE ON LABORATORY ANIMAL SCIENCE

B.S.R.C. Alexander Fleming

21 September-2 October 2009 Tel: +30 210 9656310 Fax: +30 210 9656563 Email: [email protected]

WEEK 1

Monday 21-9

Time 09.00-10.30 10.45-12.15 12.15-13.15 13.15-14.45 14.45-16.15 16.30-17.30 09.00-10.30 10.45-12.15

Tuesday 22-9

12.15-13.15 13.15-14.30 14.30-16.30

Wednesday 23-9

16.4509.00-10.30 10.45-12.15 12.15-13.15 13.30-14.30 14.30-16.45

Thursday 24-9

16.45…. 09.00-10.15 10.30-11.30 11.30-12.00 12.00-13.00 13.00-15.00 15.00-15.45 15.45-16.30 16.45….. 09.00-10.00 10.15-13.00

Friday 25-9

13.00-14.00 14.00-16.30 16.45…..

Groups Lectures /practical / demonstrations Introduction Lab. Animal Science Video & ethical discussion Lunch break International legislation From brainwave to publication: adm. constraints or quality assurance Preparation protocol, analysis scientific paper (introduction) Welfare and experimental procedures Welfare and experimental procedures (continued) Lunch break Practical training (handling) intro A+B A Practical training (handling) B Preparation protocol / selfstudy A+B Statistics + methodology I Statistics + methodology II Lunch break Breeding laboratory rodents B Practical training (handling) A Preparation protocol / selfstudy A+B Nutrition FELASA mission Embryonic Stem Cells Lunch break Demo experimental techniques Preparation protocol / selfstudy A Practical handling B Preparation protocol/selfstudy A Practical handling B Preparation protocol/selfstudy A+B Practical training(techniques)intro A+B Practical training(techniques) A Preparation protocol / selfstudy B Lunch break B Practical training(techniques) A Preparation protocol / selfstudy A+B

Lecturers Room V. Baumans V. Baumans, H. Blom H. Blom H. Blom V. Baumans, H. Blom V. Baumans V. Baumans P. Rooymans P. Rooymans, V. Baumans, H. Blom A. Kastania A.Kastania Kate Pritchett P. Rooymans, V. Baumans, H. Blom J.Meijer N. Kostomitsopoulos E.Rempoutsika P. Rooymans P. Rooymans, V. Baumans, H. Blom P. Rooymans, V. Baumans, H. Blom P. Rooymans P. Rooymans, V. Baumans, H. Blom P. Rooymans, V. Baumans, H. Blom

WEEK 2

Time 09.00-10.30 10.30-12.00

Monday 28-09

12.00-13.00 13.00-14.30 14.30-15.30 15.30-16.30 16.30….. 09.15-10.00 10.15-11.00 11.15-12.30 12.30-13.30

Tuesday 29/09

Wednesday 30-09

13.30-14.00 14.00-14.30 14.30-15.30 15.30-16.00

From the idea to the experiment : organizing and planning a new protocol

16.00-17.30

Preparation of protocol / selfstudy

09.00-10.00 10.30-11.00 11.00-11.30 11.30-12.00 12.00-13.00

10.00-12.00 12.00-13.00

Genetic standardization Forward genetics Basic principles of transgenesis in mouse Zoonoses related to lab animals Lunch break Mouse pathology I: Non infectious diseases of mice and rats Mouse pathology II: Infectious diseases of mice and rats Mouse and rat autopsy / protocol Preparation of protocol / selfstudy Mouse and rat autopsy / protocol Preparation of protocol / selfstudy Lunch break Preparation examination Examination Excursion Central Animal Facility/Alexander Fleming museum Preparation protocol / selfstudy Lunch break

13.00-16.30

Presentation protocol

13.00-15.00 15.00-17.00 9.00-10.15 Thursday 1-10

Friday 2/10

Lectures / practical / demonstrations Greek legislation/ Ethics in animal based research Alternatives to animal procedures Lunch break Cost-Benefit analysis FELASA health screeningrecommendations Microbiology - Gnotobiology Preparation protocol / selfstudy Cryopreservation Anaesthesia Analgesia, and peri-operative care Lunch break The art and science of housing laboratory mice Morphological aspects of intracrinology Methods of humane euthanasia

10.15-12.30 12.30-13.30 13.30-15.00 15.00-17.00 9.00-10.00

Groups

Lecturers K. Marinou I. Dontas K. Marinou, I. Dontas E. Petridou E. Petridou

A+B M. Alexiou P. Flecknell P. Flecknell M. Kamber A.Sourla P. Flecknell A. Papalois A+B E. Douni E. Douni D. Kontoyiannis G. Saroglou C. Brayton C. Brayton A B B A

C. Brayton C. Brayton

A+B A+B A+B M.Kamber A+B E. Douni, M. A+B Kamber, K. Marinou, C. Brayton

Room

16.30-17.00

Refreshments and Farewell

All