E05 Translation
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TRANSLATION Requirements: • • • • • • • • •
Template – mRNA for codon presentation Ribosome (rRNA + protein) 20 amino acids Initiation and elongation factors Start codon (AUG) and termination codons (UAG, UAA, UGA) Charged tRNA and anticodon presentation GTP Aminoasil-tRNA synthetase and peptidyl transferase 4 stages – tRNA charging, initiation, elongation and termination
DIFFERENCES IN TRANSCRIPTION AND TRANSLATION IN PROKARYOTE AND EUKARYOTE Prokaryote
Eukaryote
Polycistronic mRNA
Monosistronic mRNA
Exon only
Exon and intron
Transcription and translation concurrent in cytoplasm
Transcription in nucleus and translation in cytoplasm
Starting AA formyl-methionine
Starting AA methionine
Template – mRNA for codon presentation Ribosome (rRNA + protein) 20 amino acids Initiation and elongation factors Start codon (AUG) and termination codons (UAG, UAA, UGA) Charged tRNA and anticodon presentation GTP Aminoasil-tRNA synthetase and peptidyl transferase 4 stages – tRNA charging, initiation, elongation and termination
DIFFERENCES IN TRANSCRIPTION AND TRANSLATION IN PROKARYOTE AND EUKARYOTE Prokaryote
Eukaryote
Polycistronic mRNA
Monosistronic mRNA
Exon only
Exon and intron
Transcription and translation concurrent in cytoplasm
Transcription in nucleus and translation in cytoplasm
Starting AA formyl-methionine
Starting AA methionine
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