Diuresis Research Work

Taking urine and fecal probes from lab animals: problems and solutions. Stklyanina L.V., Doe Amenyo Human Anatomy Department, Lugansk Medical University. (Scientific supervisor- ass.prof. Stklyanina L.V.).

• Challenging common clinical tasks, such as monitoring serological conditions of an organism, the most obvious and initial step in this research is the analysis of the daily diuresis and urine contents. • Getting the probe of urine from the lab rats may be problematic. • First, animals during 24-hours monitoring usualy kept in the isolated chamber without water supply, that hardly influences on the urine viscosity and distorts it's chemical properties. • Second, without proper mechanical filtration the urine probes are usually mixed with the fecals, spreading by the animal over the floor of chamber.

• Third, it's difficult to collect ALL the amount of urine without special V-shape collector. • Fourth, there is no proper construction for getting both urine and fecals separately at the same 24h period. • Fifth, individual chambers for lab animals are too expensive for wide-users' demands.

So, we are intrigued by the creation of the simple construction that allows to get the urine and fecal probes and solve all those problems, mentioned above.

Our proposition is to use the 10-liters plastic bottle with the lattices double floor, putting upside down. Perforatings over the walls of bottle maintains the proper ventilation.

Floor arranged as 2 layers. Upper layer settels the animal and made up with the dural lattice (cell diameter no more than 3 mm). Important that the dural metal resists the corrosion and does not interact with urinary acids.

Forming the second layer, we used a tea-strainer, which is firmly wrapped at the container's mouth around the neck. Diameter of the cells in our teastrainer is no more than 1 mm, .so it hampers the smallest side bodies and clears the urine's efficiently.

To create a V-shape collector, we took the plastic funnel and fixed the container inside the wide part of it. Note that the end of funnel hermetically united with the lab sterile flask. It is the place where urine accumulates and further transport into the chemical laboratory for analysis.

We also provided proper water supply for animal to prevent it from dehydration. Hence, at the outer and inner surfaces on the wall of bottle we fixed the halves of syringe (5ml), which are interconnected by the plastic tube.

Such easy construction works as communicative vessels, or ducting. Outer halve, when filled by water, sends the water slowly into the inner halve. So, animal receives water ad libitum and can not overflow or spill the fluid from the drinking through because of slow release from outer vessel. Outer and inner halves were left with measurements, so you may check the initial and final (after 24h) amount of the water in it. When compared with the amount of the received urine (in ml per 24h), it is the way to calculate 24h-diuresis.

As a result, our construction houses the rat in rather comfortable personal reservoir, totally isolated from external influences and with proper ventilation and natural drinking regiment.

• So, benefits of our construction are: • 1. Our drinking-through releases the water from outer vessel into the inner one gradually and proportionaly to the water consumption by rat, so not even drop of water wouldn't be lost. So, any drug solution may be applied and the calculation of the liquor intake per hour or 24h is very easy and precise. • 2. Metal floor works as the primary filter where the fecals hampers and accumulates and does not pollute the urine, excreted freely by animal. Since that, it is easy to pick up the fecals for the further analysis.

• 3. Urine, passing through the cells of the metal floor (primary filter), flows to the neck of the container under the force of gravity. Reaching the neck, urine meets with the secondary filter of the tea-strainer, where it undergoes the careful refinement second time. Note that the cleared urine enters directly to the sterile flask, which serves as a collector and transport container of the particular probe. So, there is no need to pour the urine into another glass for the analysis. Such hermetization of the probe excludes the any interaction of the urine with the external environment and saves the yield of probe lossless. • 4. Finally, this device is attractive from an economic standpoint since it may be properly designed by the cheapest components within 2-3 hours. Daily capacity isn't restricted. Our construction is mobile and light in weight, therefore was created for both animal's and user 's comfort.

HOPE, OUR DEVICE WOULD BE USEFUL TO YOU, MY DEAR COLLEGES. THANKS A LOT FOR YOUR ATTENTION!