Dengue Report.docx

LABORATORY DEPARTMENT Concern: NON-REACTIVE results on suspected Dengue Patients Requested by: Dr. Emy Sangalang All request were subjected to proper procedure by the MTOD as required and stated by the inserts of SD Dengue NS1 + Ab Combo and Bio Tracer Dengue IgG/IgM Rapid Card. Bio Tracer Dengue IgG/IgM Rapid Card Exp Date: 12-2017 1. Add 5ul of specimen (serum, plasma or whole blood) to sample well 2. Add 4 drops of sample diluent to diluent well 3. Read after 15 – 20 minutes SD DENGUE NS1 + Ab Combo Exp Date: 01-2018 NS1 1. Add 100ul of serum, plasma or whole blood into sample well Ab Combo 1. Add 10ul of serum, plasma or whole blood into sample well marked “S” 2. Put 4 drops of assay diluent into the round-shaped assay diluent well READ after 15-20minutes All of this procedure was done properly and accurately by MTODs. With regards to the concern of NON-REACTIVE results with marked platelet drops, we can correlate it to the indications and principle of the procedures: Bio Tracer Dengue IgG/IgM Rapid Card Principle: Immunochromatography In-vitro test for the qualitative determination of dengue virus specific IgG or IgM ***If antibodies against dengue are present enough in the sample, a colored band of dengue IgG/IgM in the test zone will appear. If there are no antibodies or not sufficient in the sample, the area will remain colourless. The sample continues to move to the control reaction zone and forms a red or purple colour, indicating the test is working and result is valid SD DENGUE NS1 + Ab Combo Principle: Immunochromatography In-vitro test designed to detect both Dengue NS1 Dengue virus and differential IgG/IgM antibodies for detection in acute dengue infection. Limitation of the Test: 1. A negative result can occur if the quantity of Dengue virus NS1 antigen is present in the specimen is below the detection limits of the assay, or the antigens that are detected by the test are not present during the stage of disease in which sample is collected 2. A negative result cannot exclude a recent infection 3. In early infections and some secondary infections, detectable levels of IgM antibodies may be low. Some patients may not produce detectable levels of IgM antibodies within the first seven to 10 days after infection. Where symptoms persist, patients should be re-tested 3 – 4 days after the first specimen. Factors to consider: 1. Date of examination after disease / antigen exposure 2. Quantity of antigen and antibodies in the specimen 3. Other diseases to consider : Chikungunya