Comparison All

std curve 140

IFN

A 0 6.25 12.5 25 50 100 200

B 5.3 13.6 20.1 33.9 64.6 132.5 359

C 5.7 13.3 19.6 33.7 64.4 132.6 357.8

Av 5.6 13.8 20.2 32.9 64.3 130.7 349.4

Av-bl 5.53 13.57 19.97 33.5 64.43 131.93

f(x) = 1.26x R² = 1

120

0 8.03 14.43 27.97 58.9 126.4

Y=1.2603X - 1.4086

100 80 60 40 20 0 0

0 15.6 31.25 62.5 125 250 500 1000 2000

5.7 10 14.8 27.5 48.4 91.7 179.1 357.8 718.5

6.7 9 16.8

6.2 9.5 15.8 27.5 48.5 91.7 178.8 358 723.1

48.6 91.7 178.5 358.2 727.7

0 3.3 9.6 21.3 42.3 85.5 172.6 351.8 716.9

Y= 0.3583x - 2.7111 800

f(x) = 0.36x R² = 1

700 600 500 400 300 200 100 0

sample 3 Pr hour '2h 3Pr' '4h 3Pr' '6h 3Pr' '10h 3Pr' '14h 3Pr' '18h 3Pr' TC hour

0

A

B 13.1 4.9 23.1 48.3 146.5 53.2

A 2 4 6 10 14 18

IB hour

B 17.4 25.9 30.4 38.4 46.2 43.1

A 2 4 6 14 18

C 10.7 6.5 22.1 49.7 145.5 52.9

C 17.4 22.8 31.1 39.5 48.1 43.4

B 5.8 5.2 9.6 12.8 11.3

Av 11.5 6.4 23 49.3 143.5 53.2

Av 14.7 21.2 30.5 38.8 47.5 43.9

C 5.8 4.4 9.8 13.2 12.1

Av-bl 11.77 5.93 22.73 49.1 145.17 53.1

Av-bl 16.5 23.3 30.67 38.9 47.27 43.47

Av 6.2 4.5 10.2 13.4 11.7

5.57 -0.27 16.53 42.9 138.97 46.9

10.3 17.1 24.47 32.7 41.07 37.27

Av-bl 5.93 4.7 9.87 13.13 11.7

std dev %cv df 1.22 10.39 0.9 15.11 0.55 2.42 0.72 1.47 1.53 1.05 0.17 0.33

2 2 2 2 2 2

std dev %cv df 1.56 9.45 2.39 10.26 0.38 1.23 0.56 1.43 0.97 2.05 0.4 0.93

2 2 2 2 2 2

std dev %cv 0.4 0.23 -0.83 0.44 4.33 0.31 7.6 0.31 6.17 0.4

[IFN]

[IFN]

df 3.89 9.27 3.1 2.33 3.42

23.1 6.82 53.71 127.3 395.42 138.46

36.31 55.29 75.85 98.83 122.18 111.58

[IFN] 20 20 20 20 20

1.44 0.46 4.56 7.15 6.01

5

sample 4 Pr hour A 2 4 6 10 14 18 TC hour

B 13.9 12.1 22.2 83.7 219.2 107.9

A 2 4 6 10 14 18

IB hour

B 15.3 21.9 24.5 42.3 69.1 57.4

A 2 4 6 14 18

TC hour

Av

Av 13.4 12.7 9.6 45.4 52.4 46.9

C 17.2 43.9 4.6

0.27 0.2 3.77 14.62 9.77

Av-bl 13.63 12.67 8.8 45.83 52.47 48.03

Av 17.1 43.7 4.7

8.8 15.77 18.9 35.67 64.83 50.6

Av-bl 5.8 5.73 9.3 20.15 15.3

15.3

7.8 5.2 16.13 76.87 214.57 99.5

Av-bl 15 21.97 25.1 41.87 71.03 56.8

5.8 5.3

C

B 16.6 43.4 3.5

Av

C

13.8 12.8 9.1 46.6 52.1 48.3

Av-bl 14 11.4 22.33 83.07 220.77 105.7

14.8 21.8 25.7 41.8 71.5 56.8

5.3 6.2 9.6 20.1 15.1

B

A 2 4 6

C

B

13.7 12.5 7.7 45.5 52.9 48.9

Av 14.1 10.9 22.5 81.9 220.9 104.9

14.9 22.2 25.1 41.5 72.5 56.2

6.3 5.7 9 20.2 15.5

sample 11 Pr hour A 2 4 6 10 14 18

C 14 11.2 22.3 83.6 222.2 104.3

16.97 43.67 4.27

std dev %cv 0.1 0.62 0.15 1.01 1.5 1.93

0.71 5.48 0.68 1.22 0.68 1.82

df

std dev %cv 0.26 0.21 0.6 0.4 1.75 0.6

1.76 0.95 2.39 0.97 2.46 1.06

std dev %cv 0.5 0.45 0.42 0.07 0.2

8.62 7.86 4.56 0.35 1.31

[IFN] 2 2 2 2 2 2

df

[IFN] 2 2 2 2 2 2

df

32.13 51.57 60.32 107.11 188.51 148.79

[IFN] 20 20 20 20 20

std dev %cv df 0.21 1.53 0.15 1.21 0.98 11.19 0.67 1.45 0.4 0.77 1.03 2.14

2 2 2 2 2 2

Av-bl std dev %cv df 10.77 0.32 1.89 37.47 0.25 0.58 -1.93 0.67 15.61

2 2 2

7.43 6.47 2.6 39.63 46.27 41.83

29.34 22.08 52.59 222.1 606.41 285.27

1.33 1.28 4.11 12.72 8.87

[IFN] 28.31 25.61 14.82 118.18 136.69 124.32

[IFN] 37.62 112.13 2.17

10 14 18 IB hour

62 76.6 70.7

A 2 4 6 14 18

61.1 77.6 69.4

B 5.1 8 18.1 25 14.5

61.1 77.2 70.7

C 5.2 8.7 17.7 24.7 14.4

61.4 77.13 70.27

Av 5.4 7.6 17.3 24.8 14.7

55.2 70.93 64.07

0.52 0.5 0.75

Av-bl 5.23 8.1 17.7 24.83 14.53

std dev %cv -0.3 0.15 2.57 0.56 12.17 0.4 19.3 0.15 9 0.15

0.85 0.65 1.07

2 2 2

df 2.92 6.87 2.26 0.62 1.05

161.63 205.54 186.37

[IFN] 20 20 20 20 20

0.88 3.15 10.77 16.43 8.26

sample 12 Pr hour

A 2 4 6 10 14 18

TC hour

B 19.1 19.5 16.6 57.5 55.2 78.6

A 2 4 6 10 14 18

IB hour

18.6 20 15.3 56.8 56 80

B 22.6 45.3 45.8 69.7 83.9 80.7

A 2 4 6 14 18

C 17.9 19.5 15.2 56.6 55.6 78.4

C 22.1 45.2 44.7 70.9 83.1 80.6

B 7.1 12.2 12.5 16.4 19.2

Av

3.25 1.47 4.97 0.83 0.72 1.1

Av-bl std dev %cv 16.07 0.29 39 0.1 38.97 0.57 64.2 0.62 77.47 0.49 74.57 0.21

1.3 0.22 1.26 0.89 0.59 0.26

Av 22.1 45.1 45 70.6 84 81

C 6.5 13.9 12.6 15.8 19.7

Av-bl std dev %cv 18.53 12.33 0.6 19.67 13.47 0.29 15.7 9.5 0.78 56.97 50.77 0.47 55.6 49.4 0.4 79 72.8 0.87

22.27 45.2 45.17 70.4 83.67 80.77

Av 7 14.1

Av-bl 6.87 13.4 12.55 16.1 19.33

19.1

1.33 7.87 7.02 10.57 13.8

std dev %cv 0.32 1.04 0.07 0.42 0.32

df

[IFN] 2 2 2 2 2 2

df

[IFN] 2 2 2 2 2 2

df 4.68 7.79 0.56 2.64 1.66

41.99 45.15 34.08 149.25 145.44 210.75

52.41 116.41 116.32 186.75 223.77 215.68

[IFN] 20 20 20 20 20

2.18 7.36 6.69 9.5 12.07

sample 7 Pr hour

A 2 4 6 10 14

B 47.5 69.4 59.7 161.2 110.2

C 47 70 58.2 160.4 109.9

Av 46.5 69.2 57.4 160 109.1

Av-bl 47 69.53 58.43 160.53 109.73

std dev %cv df 40.8 0.5 1.06 63.33 0.42 0.6 52.23 1.17 2 154.33 0.61 0.38 103.53 0.57 0.52

[IFN] 2 121.44 2 184.33 2 153.35 2 438.3 2 296.52

18 TC hour

101

A 2 4 6 10 14 18

IB hour

B 24.5 42.3 37.5 58 66.6 59.4

A 2 4 6 14 18

IB hour

66.3 65.1 92.3 127.2 108.7 50.4

sample 15

Av

Av

C

B

Av-bl

Av-bl 28 49.03 36.27 57.87 63.4 55.47

Av 26.4

13.1

21.3 6.07 4.47 18.5 9.37

0.38

21.8 42.83 30.07 51.67 57.2 49.27

Av-bl 25.73 12.3 11.55 18.25 12.97

20.2 6.77 6.02 12.72 7.43

0.37

std dev %cv 0.53 0.31 0.44 0.25 0.12 0.79

2.11 0.72 1.17 0.44 0.17 1.36

std dev %cv 1.03 0.2 0.1 0.42 0.44

3.82 1.72 1 1.73 2.93

Av-bl std dev %cv 65.97 59.77 0.42 64.5 58.3 1.22 91.07 84.87 1.12 126.3 120.1 1.08 109.4 103.2 0.61 49.53 43.33 0.85

Av 27.9 48.1 35.8 58.6 64.2 54.9

C 27 12.9 11.5 18.4 12.8

18.9 36.37 31.1 51.53 60.47 52.3

26.83 11.6 10 24.03 14.9

65.5 63.1 90.1 125.1 109.7 48.7

27.9 48.8 36.6 57.6 62.7 55.2

95.23

Av-bl 25.1 42.57 37.3 57.73 66.67 58.5

27.1 11.6 9.9 23.9 15.4

C

B

23.8 11.7 11.6 18.1 13

Av

C

66.1 65.3 90.8 126.6 109.8 49.5

28.2 50.2 36.4 57.4 63.3 56.3

101.43

25.3 42.5 36.8 57.7 66.6 58.2

27.7 11.4 10.1 23.7 14.6

B

A 2 4 6 14 18

C

B

A 2 4 6 10 14 18

101.7

25.5 42.9 37.6 57.5 66.8 57.9

25.7 11.8 10 24.5 14.7

sample 8 Pr hour A 2 4 6 10 14 18 TC hour

101.6

2

df

[IFN] 2 2 2 2 2 2

df

0.63 1.89 1.23 0.86 0.56 1.72

0.62 2.18 1.15 1.11 1.19 1.33

std dev %cv 1.7 0.85 0.07 0.21 0.15

6.61 6.9 0.61 1.16 1.18

60.32 109.06 94.37 151.39 176.33 153.53

[IFN] 20 20 20 20 20

df

std dev %cv 0.17 1.07 0.42 0.64 0.75 0.74

273.36

18.02 5.93 4.66 15.8 8.55

[IFN] 2 174.37 2 170.28 2 244.43 2 342.76 2 295.59 2 128.51

df

[IFN] 2 2 2 2 2 2

df

68.41 127.11 91.48 151.77 167.21 145.07

[IFN] 20 20 20 20 20

17.15 6.49 5.89 11.21 7.02

Pr hour

A 2 4 6 10 14 18

TC hour

B 49.5 76.9 66.2 89.2 103.7 45.2

A 2 4 6 10 14 18

IB hour

B 29.9 53.2 46.3 78.4 70.5 77.3

A 2 4 6 14 18

C 49 76.7 65.7 88.9 103.7 45.4

C 30.3 54 46.8 78.1 70.8 76.2

B 16.8 11.1 13.9 23.8 13.8

Av 48.6 76.4 64.4 89.8 102.4 45.1

49.03 76.67 65.43 89.3 103.27 45.23

Av 29.8 52.9 47.1 78.6 70.8 76.1

C 16 10.6 13.3 23.9 13.8

Av

std dev %cv 23.8 0.26 47.17 0.57 40.53 0.4 72.17 0.25 64.5 0.17 70.33 0.67

df 0.92 0.33 1.42 0.51 0.73 0.34

Av-bl 30 53.37 46.73 78.37 70.7 76.53

17.1 11 13.1 24.5 13.7

Av-bl std dev %cv 42.83 0.45 70.47 0.25 59.23 0.93 83.1 0.46 97.07 0.75 39.03 0.15

Av-bl 16.63 10.9 13.43 24.07 13.77

11.1 5.37 7.9 18.53 8.23

2 2 2 2 2 2

df 0.88 1.07 0.86 0.32 0.24 0.87

std dev %cv 0.57 0.26 0.42 0.38 0.06

3.42 2.43 3.1 1.57 0.42

std dev %cv 1.06 1.05 1.15 0.29 0.4 0.4

2.16 1.6 1.82 0.31 0.51 0.82

std dev %cv 0.44 0.23 0.3 0.62 2.52 0.42

0.98 0.41 0.57 0.7 2.02 0.47

[IFN] 127.11 204.24 172.88 239.5 278.48 116.51

[IFN] 2 2 2 2 2 2

df

73.99 139.21 120.69 208.98 187.58 203.86

[IFN] 20 20 20 20 20

9.93 5.38 7.39 15.82 7.65

sample 16 Pr hour

A 2 4 6 10 14 18

TC hour

A 2 4 6 10 14 18

IB hour

B 49.9 66.9 64.5 94.3 80 49.4

B 45.1 56.9 53 89.4 127.6 88.5

A

C 49.5 65.8 63.3 94.8 79.7 49

C 44.4 56.5 52.4 89.1 122.6 87.7

B

Av 47.9 64.8 62.2 94.3 79.2 48.6

Av 44.3 56.9 52.7 90.3 124.5 87.9

C

Av-bl 49.1 65.83 63.33 94.47 79.63 49

Av-bl 44.6 56.77 52.7 89.6 124.9 88.03

Av

42.9 59.63 57.13 88.27 73.43 42.8

38.4 50.57 46.5 83.4 118.7 81.83

Av-bl

std dev

%cv

df

[IFN] 2 2 2 2 2 2

127.3 174 167.02 253.92 212.52 127.02

2 2 2 2 2 2

[IFN] 114.74 148.7 137.35 240.33 338.85 235.96

df

df

[IFN]

2 4 6 14 18

12.9 11.9 14.2 27.6 16.5

12.6 12.1 13.6 27.7 16.1

12.3 12

16.2

12.6 12 13.9 27.65 16.27

7.07 6.47 8.37 22.12 10.73

0.3 0.1 0.42 0.07 0.21

2.38 0.83 3.05 0.26 1.28

20 20 20 20 20

6.72 6.25 7.76 18.67 9.63

std curve (lower) 140

f(x) = 1.26x - 1.41 R² = 1

120 100 80 60 40 20 0 0

50

100

150

200

250

2000

2500

std curve (higher) 800

f(x) = 0.36x - 2.71 R² = 1

700 600 500 400 300 200 100 0 0

500

1000

1500

/10 [IFN]*df /2 repeat actual 46.21 23.1 2.31 13.64 6.82 below limit 20.86 0.68 107.42 53.71 5.37 254.6 127.3 12.73 790.83 395.42 39.54 276.92 138.46 13.85

[IFN]*df /2 actual 72.63 36.31 3.63 110.58 55.29 5.53 151.7 75.85 7.59 197.66 98.83 9.88 244.36 122.18 12.22 223.15 111.58 11.16

[IFN]*df /2 28.7 9.13 91.12 142.96 120.21

actual 14.35 1.44 4.56 0.46 45.56 4.56 71.48 7.15 60.11 6.01

0

[IFN]*df /2 actual 58.67 29.34 2.93 44.16 22.08 2.21 105.19 52.59 5.26 444.2 222.1 22.21 1212.83 606.41 60.64 570.53 285.27 28.53

[IFN]*df /2 actual 64.25 32.13 3.21 103.14 51.57 5.16 120.63 60.32 6.03 214.22 107.11 10.71 377.03 188.51 18.85 297.58 148.79 14.88

[IFN]*df /2 actual 26.59 13.29 1.33 25.53 12.76 1.28 82.13 41.06 4.11 254.31 127.15 12.72 177.34 88.67 8.87 0

[IFN]*df /2 56.63 28.31 51.23 25.61 29.65 14.82 below limit 236.36 118.18 273.39 136.69 248.64 124.32

actual 2.83 2.56 26.75 2.68 11.82 13.67 12.43

[IFN]*df /2 75.23 37.62 224.27 112.13 4.34 2.17 below limit

actual 3.76 11.21 0.22

323.25 411.08 372.75

161.63 205.54 186.37

16.16 20.55 18.64

[IFN]*df /2 actual 17.59 8.8 0.88 63.08 31.54 3.15 215.43 107.71 10.77 328.63 164.31 16.43 165.18 82.59 8.26 0

[IFN]*df /2 actual 83.98 41.99 4.2 90.3 45.15 4.52 68.16 34.08 3.41 298.51 149.25 14.93 290.88 145.44 14.54 421.5 210.75 21.07

[IFN]*df /2 104.82 232.83 232.64 373.49 447.55 431.36

52.41 116.41 116.32 186.75 223.77 215.68

actual 5.24 11.64 11.63 18.67 22.38 21.57

[IFN]*df /2 actual 43.51 21.76 2.18 147.19 73.6 7.36 133.7 66.85 6.69 190.04 95.02 9.5 241.35 120.67 12.07 0

[IFN]*df /2 242.88 368.65 306.7 876.61 593.05

actual 121.44 12.14 184.33 18.43 153.35 15.33 438.3 43.83 296.52 29.65

12.14

24.29

546.72

[IFN]*df /2 120.63 218.13 188.73 302.79 352.65 307.07

273.36

27.34

60.32 109.06 94.37 151.39 176.33 153.53

actual 6.03 10.91 9.44 15.14 17.63 15.35

[IFN]*df /2 actual 360.37 180.18 18.02 118.63 59.31 5.93 93.24 46.62 4.66 315.93 157.97 15.8 171 85.5 8.55

[IFN]*df /2 348.75 340.56 488.85 685.52 591.19 257.02

actual 174.37 17.44 170.28 17.03 244.43 24.44 342.76 34.28 295.59 29.56 128.51 12.85

[IFN]*df /2 136.82 254.23 182.96 303.53 334.42 290.14

68.41 127.11 91.48 151.77 167.21 145.07

actual 6.84 12.71 9.15 15.18 16.72 14.51

[IFN]*df /2 actual 342.91 171.46 17.15 129.74 64.87 6.49 117.83 58.92 5.89 224.16 112.08 11.21 140.31 70.16 7.02

[IFN]*df /2 254.23 408.47 345.77 478.99 556.95 233.01

[IFN]*df /2 147.98 278.41 241.39 417.96 375.17 407.73

127.11 204.24 172.88 239.5 278.48 116.51

actual 12.71 20.42 17.29 23.95 27.85 11.65

actual 73.99 139.21 120.69 208.98 187.58 203.86

7.4 13.92 12.07 20.9 18.76 20.39

[IFN]*df /2 actual 198.5 99.25 9.93 107.52 53.76 5.38 147.72 73.86 7.39 316.46 158.23 15.82 153.01 76.51 7.65

[IFN]*df /2 254.6 348 334.05 507.83 425.03 254.04

/10 actual 127.3 12.73 174 17.4 167.02 16.7 253.92 25.39 212.52 21.25 127.02 12.7

[IFN]*df /2 229.48 297.39 274.69 480.66 677.71 471.92

114.74 148.7 137.35 240.33 338.85 235.96

[IFN]*df

/2

actual 11.47 14.87 13.73 24.03 33.89 23.6

actual

134.5 124.97 155.13 373.33 192.68

67.25 62.49 77.56 186.66 96.34

6.72 6.25 7.76 18.67 9.63

std curve IFN

140

A

B

C

0 6.25 12.5 25 50 100 200

5.3 13.6 20.1 33.9 64.6 132.5 359

5.7 13.3 19.6 33.7 64.4 132.6 357.8

0 15.6 31.25 62.5 125 250 500 1000 2000

5.7 10 14.8 27.5 48.4 91.7 179.1 357.8 718.5

6.7 9 16.8

Av 5.6 13.8 20.2 32.9 64.3 130.7 349.4

Av-bl 5.53 13.57 19.97 33.5 64.43 131.93

0 8.03 14.43 27.97 58.9 126.4

f(x) = 1.26x - 1.4 R² = 1

120

Y=1.2603X - 1.4086

100 80 60 40 20 0 0

6.2 9.5 15.8 27.5 48.5 91.7 178.8 358 723.1

48.6 91.7 178.5 358.2 727.7

0 3.3 9.6 21.3 42.3 85.5 172.6 351.8 716.9

Y= 0.3583x - 2.7111

800

f(x) = 0.36x - 2.7 R² = 1

700 600 500 400 300 200 100 0 0

pr 4h pr 23' 4h pr 24' 6h Pr 23' 6h Pr 24' 8h pr 23' 8h pr 24' 10h pr 23' 10h pr 24' 14h pr 23' 14h pr 24' 18h pr 23' 18h pr 24'

901.5 805.8 1252.9 1027 868.5 786.9 299.2 249.1 1404.6 1230.6 1238.6 1224.3

900.4 806.1 1184.2 1038.7 867.8 786.9 306.2 250.6 1253 1225.2 1230.5 1224.3

903.2 817.2 1167.5 1036.5 854.8 780.5 301.7 242.8 1235.5 1219.4 1228.5 1223.2

av

tc 4h tc 23' 4h tc 24' 6h tc 23' 6h tc 24' 8h tc 23' 8h tc 24' 10h tc 23' 10h tc 24' 14h tc 23' 14h tc 24' 18h tc 23'

484.4 849.1 897.3 852.6 851.3 1058.9 347.4 329.9 1377.8 1067.2 1201.8

688.8 803.3 910.1 866.9 850.7 961.7 359.5 329 1233.2 1087.1 1190.7

786.1 849.7 894.8 859.2 842.4 928.3 354.2 333.1 1202 1073.6 1182.1

901.70 809.70 1175.85 1034.07 863.70 784.77 302.37 247.50 1297.70 1225.07 1232.53 1223.93

av-blank std dev %cv 895.50 1.41 803.50 6.50 1169.65 11.81 1027.87 6.22 857.50 7.72 778.57 3.70 296.17 3.55 241.30 4.14 1291.50 92.99 1218.87 5.60 1226.33 5.35 1217.73 0.64

[IFN] df 2506.87 2250.10 3272.01 2876.30 2400.81 2180.51 834.16 681.02 3612.09 3409.37 3430.21 3406.21

2 2 2 2 2 2 2 2 2 2 2 2

653.10 834.03 900.73 859.57 848.13 982.97 353.70 330.67 1271.00 1075.97 1191.53

av-blank std dev %cv [IFN] df 646.90 153.99 23.58 1813.04 827.83 26.62 3.19 2318.01 894.53 8.21 0.91 2504.17 853.37 7.16 0.83 2389.28 841.93 4.97 0.59 2357.37 976.77 67.85 6.90 2733.68 347.50 6.07 1.71 977.42 324.47 2.15 0.65 913.14 1264.80 93.80 7.38 3537.57 1069.77 10.16 0.94 2993.24 1185.33 9.88 0.83 3315.78

2 2 2 2 2 2 2 2 2 2 2

av

0.16 0.80 1.00 0.60 0.89 0.47 1.17 1.67 7.17 0.46 0.43 0.05

18h tc 24'

1154.4

ib

1166

1158.2

1159.53

1153.33

5.91

0.51

3226.47

2

Y=1.2603X - 1.4086

Sample 8h ib 23' 8h ib 24' 10h ib 23' 10h ib 24' 14h ib 23' 14h ib 24 18h ib 23' 18h ib 24'

av 234.2 214 69.6 64.3 481 447.4 472.3 623.5

sample

254.5 209.4 73.7 70.6 464.4 442.7 471.3 619.6

pr

tc

av-blank std dev %cv 244.35 238.82 14.35 211.70 206.17 3.25 71.65 66.12 2.90 67.45 61.92 4.45 472.70 467.17 11.74 445.05 439.52 3.32 471.80 466.27 0.71 621.55 616.02 2.76

ib

temp

OD

[IFN] df 5.87 190.61 1.54 164.7 4.05 53.58 6.60 50.25 2.48 371.8 0.75 349.86 0.15 371.08 0.44 489.9

iptg

23

8 10 14 18

240.08 83.42 361.21 343.02

235.74 97.74 353.76 331.58

190.61 53.58 371.8 371.08

25

4

1.03

24

8 10 14 18

218.05 68.1 340.94 340.62

273.37 91.31 299.32 322.65

164.7 50.25 349.86 389.9

25

4

1.03

sample 20

OD 600 DCW temp 13.142 7.695 11.798 6.874

OD

iptg

25

8

1.03

21

13.840 11.200

8.122 6.509

25

4

0

22

14.610 11.822

8.592 6.889

25

4

2.66

rpt (200209) 26 14h pr 25 14h pr 22 14h pr 26 18h pr 25 18h pr 22 18h pr 26 14h tc 25 14h tc 22 14h tc 26 18h tc

av 625 652.8 687.9 766.5 705.6 693.9 424.2 450.8 600 638.6

620.1 646.9 690.4 689.6 687.4 687.6 447.6 481.1 548.9 629.1

20 20 20 20 20 20 20 20

av-blank std dev %cv 622.55 616.35 3.46 649.85 643.65 4.17 689.15 682.95 1.77 728.05 721.85 54.38 696.50 690.30 12.87 690.75 684.55 4.45 435.90 429.70 16.55 465.95 459.75 21.43 574.45 568.25 36.13 633.85 627.65 6.72

[IFN] df 0.56 1727.77 0.64 1803.97 0.26 1913.65 7.47 2022.22 1.85 1934.16 0.64 1918.12 3.80 1206.84 4.60 1290.71 6.29 1593.53 1.06 1759.31

[ifn]*df 2 3455.55 2 3607.93 2 3827.30 2 4044.44 2 3868.33 2 3836.23 2 2413.68 2 2581.42 2 3187.06 2 3518.62

25 18h tc 22 18h tc

477.1 498.4

471.9 447.2

22 14h ib 25 14h ib 26 14h ib 22 18h ib 25 18h ib 26 18h ib

3.4 2.6 9.2 10.3 11.7 10.5

3.4 7.2 9.7 12.1 12.1 10.3

474.50 472.80

av 3.4 4.9 9.45 11.2 11.9 10.4

468.30 466.60

3.68 36.20

0.77 7.66

1314.57 1309.83

av-blank std dev %cv [IFN] df -2.13 0.00 0.00 -0.58 -0.63 3.25 66.38 0.62 3.92 0.35 3.74 4.23 5.67 1.27 11.36 5.61 6.37 0.28 2.38 6.17 4.87 0.14 1.36 4.98

2 2

2629.14 2619.65

20 20 20 20 20 20

[ifn]*df -11.50 12.30 84.51 112.28 123.39 99.58

140

f(x) = 1.26x - 1.41 R² = 1

120 100 80 60 40 20 0 0 800

50

100

150

200

250

1000

1500

2000

2500

f(x) = 0.36x - 2.71 R² = 1

700 600 500 400 300 200 100 0 0

500

/2 /10 [ifn]*df conc sample actual [ifn] 5013.74 2506.87 250.69 4500.20 2250.10 225.01 6544.02 3272.01 327.20 5752.60 2876.30 287.63 4801.62 2400.81 240.08 4361.03 2180.51 218.05 1668.31 834.16 83.42 1362.05 681.02 68.10 7224.18 3612.09 361.21 6818.74 3409.37 340.94 6860.42 3430.21 343.02 6812.42 3406.21 340.62 /2 /10 [ifn]*df conc sample actual [ifn] 3626.07 1813.04 181.30 4636.03 2318.01 231.80 5008.34 2504.17 250.42 4778.55 2389.28 238.93 4714.73 2357.37 235.74 5467.36 2733.68 273.37 1954.85 977.42 97.74 1826.28 913.14 91.31 7075.14 3537.57 353.76 5986.48 2993.24 299.32 6631.56 3315.78 331.58

6452.94

3226.47

322.65

/2 /10 [ifn]*df conc sample actual [ifn] 3812.19 1906.10 190.61 3294.06 1647.03 164.70 1071.57 535.79 53.58 1004.92 502.46 50.25 7435.93 3717.97 371.80 6997.15 3498.57 349.86 7421.65 3710.82 371.08 9798.07 4899.03 489.90

/2 /10 conc sample actual [ifn] 1727.77 172.78 1803.97 180.40 1913.65 191.37 2022.22 202.22 1934.16 193.42 1918.12 191.81 1206.84 120.68 1290.71 129.07 1593.53 159.35 1759.31 175.93

rpt (200209)actual [ifn] 26 14h pr 172.78 25 14h pr 180.4 22 14h pr 191.37 26 18h pr 202.22 25 18h pr 193.42 22 18h pr 191.81 26 14h tc 120.68 25 14h tc 129.07 22 14h tc 159.35 26 18h tc 175.93

1314.57 1309.83

131.46 130.98

/2 /10 conc sample actual [ifn] -5.75 -0.58 6.15 0.62 42.25 4.23 56.14 5.61 61.69 6.17 49.79 4.98

25 18h tc 22 18h tc

131.46 130.98

22 14h ib 25 14h ib 26 14h ib 22 18h ib 25 18h ib 26 18h ib

/10 actual [ifn] -0.58 0.62 4.23 5.61 6.17 4.98

sample

hour

pr

tc

temp

OD

iptg

1

8 10 14 18

8.93 17.7 9.34 7.99

2.31 9.13 3.49 5.43

0 3.59 4.82 0

20

1

0.05

3

2 4 6 10 14 18

2.31 0.68 5.37 12.73 39.54 13.85

3.63 5.53 7.59 9.88 12.22 11.16

1.44 0.46 4.56

30

7

0.05

2 4 6 10 14 18

2.93 2.21 5.26 22.21 60.64 28.53

3.21 5.16 6.03 10.71 18.85 14.88

1.33 1.28 4.11

30

7

0.05

12.72 8.87

5

8 10 14 18

13.33 20.07 59.15 148.29

36.36 15.43 18.45 55.81

11.84 4.82 3.83 17

20

1

0.05

6

8 10 14 18

40.43 31.88 74.05 119.12

25.86 8.08 21.94 44.18

14.62 6.61 14.66 11.64

20

1

0.05

7

2 4 6 10 14 18

12.14 18.43 15.33 43.83 29.65 27.34

6.03 10.91 9.44 15.14 17.63 15.35

18.02 5.93 4.66

30

7

0.05

2 4 6 10 14 18

17.44 17.03 24.44 34.28 29.56 12.85

6.84 12.71 9.15 15.18 16.72 14.51

17.15 6.49 5.89

30

7

0.05

8 10 14 18

7.59 30.56 9.5 9.6

6.6 15.48 21.81 28.02

5.73 0.46 2.52 2.32

20

1

2

4

8

9

7.15 6.01

15.8 8.55

11.21 7.02

10

10 14 18

27.34 12.68 16.64

11.77 34.42 32.05

4.3 5.85 0.65

20

1

2

11

2 4 6 10 14 18

2.83 2.56 2.68 11.82 13.67 12.43

3.76 11.21 0.22 16.16 20.55 18.64

0.88 3.15 10.77

30

7

2

2 4 6 10 14 18

4.2 4.52 3.41 14.93 14.54 21.07

5.24 11.64 11.63 18.67 22.38 21.57

2.18 7.36 6.69

30

7

2

9.5 12.07

8 10 14 18

18.66 33.36 56.67 194.04

19.82 6.53 11.09 21.68

20

1

2

23.7 32.13 81.42

8 10 14 18

31.31 108.6 97.02 244.89

26.52 6.45 6.96 16.68

20

1

2

19.22 36.06 58.08

2 4 6 10 14 18

12.71 20.42 17.29 23.95 27.85 11.65

7.4 13.92 12.07 20.9 18.76 20.39

9.93 5.38 7.39

30

7

2

2 4 6 10 14 18

12.73 17.4 16.7 25.39 21.25 12.7

11.47 14.87 13.73 24.03 33.89 23.6

30

7

2

12

13

14

15

16

sample

pr 23

8 10 14 18

tc 240.08 83.42 361.21 343.02

16.43 8.26

15.82 7.65 6.72 6.25 7.76 18.67 9.63 ib

235.74 97.74 353.76 331.58

temp 190.61 53.58 371.8 371.08

OD 25

iptg 4

1.03

24

8 10 14 18

218.05 68.1 340.94 340.62

273.37 91.31 299.32 322.65

164.7 50.25 349.86 389.9

25

4

1.03

IFN

A

B

C

Av 5.6 13.8 20.2 32.9 64.3 130.7 349.4

Av-bl

0 6.25 12.5 25 50 100 200

5.3 13.6 20.1 33.9 64.6 132.5 359

5.7 13.3 19.6 33.7 64.4 132.6 357.8

5.533 13.567 19.967 33.500 64.433 131.933

0 8.033 14.433 27.967 58.900 126.400

Y=1.2603X - 1.4086

0 15.6 31.25 62.5 125 250 500 1000 2000

5.7 10 14.8 27.5 48.4 91.7 179.1 357.8 718.5

6.7 9 16.8

6.2 9.5 15.8 27.5 48.5 91.7 178.8 358 723.1

0 3.3 9.6 21.3 42.3 85.5 172.6 351.8 716.9

Y= 0.3583x - 2.7111

48.6 91.7 178.5 358.2 727.7

pr 8h pr 1 8h pr 9 '8h pr 5' '8h pr 6' '8h pr 13' '8h pr 14'

103.8 89.5 47.3 151.5 69.9 114.7

119.1 103.4 52.6 146.8 71 117.2

129 108.4 53.9 146.8 70.1 115.1

Av av-bl std dev %cv df 117.300 111.100 12.696 10.824 100.433 94.233 9.793 9.751 51.267 45.067 3.496 6.820 148.367 142.167 2.714 1.829 70.333 64.133 0.586 0.833 115.667 109.467 1.343 1.161

2 2 2 2 2 2

10h pr 1 10h pr 9 10h pr 10 '10h pr 5' '10h pr 6' '10h pr 13' '10h pr 14'

208.4 378.2 334 75.6 117.4 123.8 395.7

230.2 383.9 348.7 75.8 117.1 122.5 392.3

245 407.7 365.5 74.8 118.6 122.7 389.8

Av av-bl std dev %cv 227.867 221.667 18.411 389.933 383.733 15.648 349.400 343.200 15.762 75.4 69.2 0.53 117.7 111.5 0.79 123 116.8 0.7 392.6 386.4 2.96

[ifn] 2 177 2 305.6 2 273.43 2 200.701 2 318.758 2 333.550 2 1085.992

'14h pr 1' '14h pr 9' '14h pr 10' '14h pr 5' '14h pr 6' '14h pr 13' '14h pr 14'

125.7 37.1 50 216.4 268.1 209.2 357.8

118 37.9 48.9 215.7 269.1 203.9 344.4

'18h pr 1' '18h pr 9'

33.9 38.5

30.3 37.3

Av 37.6 47.9 214.2 269.2

121.85 37.533 48.933 215.433 268.800 206.550 351.100 Av 32.1 37.9

av-bl std dev %cv 116.32 5.44 31.333 0.404 42.733 1.050 209.233 1.124 262.600 0.608 200.350 3.748 344.900 9.475

df 8.080 4.013 4.511 0.7 0.67 0.57 0.75

[ifn]

df 4.47 1.077 2.147 0.522 0.226 1.814 2.699

av-bl std dev %cv df 25.900 2.546 7.930 31.700 0.849 2.239

89.27 75.89 133.346 404.348 186.560 313.083

[ifn] 2 2 2 2 2 2 2

93.41 95.017 126.833 591.528 740.472 566.735 970.168 [ifn]

2 2

79.852 96.040

'18h pr 10' 64.3 61.9 '18h pr 5' 549.8 519.8 '18h pr 6' 435.4 425.2 '18h pr 13' 724.1 673.4 18h pr 14 (10x)' ib 627.6 template 618.8

617.8

tc Sample 8h1TC 8h9TC 8h5TC 8h6TC

63.1 534.8 430.3 698.75 621.4

av

56.900 528.600 424.100 692.550 615.87

av-bl

1.697 21.213 7.212 35.850 5.393

2.689 3.967 1.676 5.131 0.868

2 166.372 2 1482.867 2 1191.212 2 1940.444 10 489.784

RelResp 11.4 27.9 130.5 100.2

12.6 27.6 147.7 96

11.3 25.9 123.1 92.2

11.767 27.133 133.767 96.133

5.567 20.933 127.567 89.933

std dev %cv 0.723 1.079 12.621 4.002

6.148 3.975 9.435 4.163

df 2 2 2 2

[ifn] 23.103 65.991 363.600 258.567

10h1TC 10h9TC 10h10TC 10h5TC 10h6TC 10h13TC 14h10TC1

37.5 58.5 47.7 57.6 29.8 86.7 70.2

36 59.5 45.4 58.7 33.1 89.1 73.6

35.1 58.9 43.9 60 34.4 89.4 73.3

36.200 58.967 45.667 58.767 32.433 88.400 72.367

30.000 52.767 39.467 52.567 26.233 82.200 66.167

1.212 0.503 1.914 1.201 2.371 1.480 1.882

3.349 0.854 4.191 2.044 7.311 1.674 2.601

2 2 2 2 2 2 2

91.295 154.836 117.716 154.278 80.783 236.983 192.235

14h1TC 14h9TC 14h10TC2 14h5TC 14h6TC 14h13TC 14h14TC

17.1 80.2 116.4 67.4 74 119.1 131

15.4 82.9 133.4 70.9 86.5 116.1 133.8

15.5 81.8 130.7 70.5 85.8 120.6 133.3

16.000 81.633 126.833 69.600 82.100 118.600 132.700

9.800 75.433 120.633 63.400 75.900 112.400 126.500

0.954 1.358 9.136 1.916 7.024 2.291 1.493

5.962 1.663 7.203 2.752 8.555 1.932 1.125

2 2 2 2 2 2 2

34.918 218.098 344.249 184.513 219.400 321.270 360.623

18h1TC 18h9TC 18h10TC 18h5TC 18h6TC 18h13TC 18h14TC

22.3 104.9 113 197.6 160.3 296.8 219.9

23.1 105.2 122.2 206.1 162.4 297.1 210

23.4 101.6 119.8 206.7 162.7 291.7 204.9

22.933 103.900 118.333 203.467 161.800 295.200 211.600

16.733 97.700 112.133 197.267 155.600 289.000 205.400

0.569 1.997 4.772 5.090 1.308 3.035 7.627

2.479 1.923 4.033 2.501 0.808 1.028 3.604

2 2 2 2 2 2 2

54.269 280.243 320.526 558.129 441.840 814.153 580.829

Y=1.2603X - 1.4086 ib Sample '8h ib 1' '8h ib 9' '8h ib 5' '8h ib 6'

RelResp 1.3 10.3 23.7 25

av 6.7 12.4 14.4 20.1

4.000 11.350 19.050 22.550

av-bl std dev %cv df -1.533 3.818 95.459 5.817 1.485 13.083 13.517 6.576 34.520 17.017 3.465 15.365

[ifn] 20 20 20 20

-0.1 5.73 11.84 14.62

'8h ib 13' '8h ib 14'

31.8 38.4

26.4 36.7

29.100 37.550

23.567 32.017

3.818 1.202

13.122 3.201

20 20

19.82 26.52

'10h ih 1' '10h ib 9' '10h ib 10' '10h ib 5' '10h ib 6' '10h ib 13' '10h ib 14'

8.6 6.7 11.1 7.4 13.3 13.1 13.2

8.7 2.7 8 13 11.6 11.6 11.3

8.650 4.700 9.550 10.200 12.450 12.350 12.250

3.117 -0.833 4.017 4.667 6.917 6.817 6.717

0.071 2.828 2.192 3.960 1.202 1.061 1.344

0.817 60.179 22.953 38.822 9.655 8.588 10.967

20 20 20 20 20 20 20

3.59 0.46 4.3 4.82 6.61 6.53 6.45

'14h ib 1' '14h ib 9' '*14h ib 10' '*14h ib 5' '*14h ib 6' '*14h ib 13' '*14h ib 14'

11.9 7.1 14 8.5 22.3 19 13.5

8.5 7.5 9 9.4 22.9 17.2 12.3

10.200 7.300 11.500 8.950 22.600 18.100 12.900

4.667 1.767 5.967 3.417 17.067 12.567 7.367

2.404 0.200 3.536 0.636 0.424 1.273 0.849

23.570 2.740 30.744 7.111 1.877 7.032 6.578

20 20 20 20 20 20 20

4.82 2.52 5.85 3.83 14.66 11.09 6.96

'*18h ib 1' '*18h ib 9' '*18h ib 10' '*18h ib 5' '*18h ib 6' '*18h ib 13' '*18h ib 14'

4.8 7.2 3.8 27 19.6 31.8 24.1

2.8 6.9 6.1 24.1 18 31.1 26.2

3.80 7.05 4.95 25.55 18.80 31.45 25.15

-1.733 1.517 -0.583 20.017 13.267 25.917 19.617

1.41 0.21 1.30 2.05 1.13 0.49 1.48

37.22 3.01 26.26 8.03 6.02 1.57 5.90

20 20 20 20 20 20 20

-0.26 2.32 0.65 17 11.64 21.68 16.68

sample

7.3

6

pr

tc

ib

1

8 10 14 18

8.93 17.7 9.34 7.99

2.31 9.13 3.49 5.43

0 3.59 4.82 0

9

8 10 14 18

7.59 30.56 9.5 9.6

6.6 15.48 21.81 28.02

5.73 0.46 2.52 2.32

10

8 10 14 18

27.34 12.68 16.64

11.77 34.42 32.05

4.3 5.85 0.65

5

8 10 14 18

13.33 20.07 59.15 148.29

36.36 15.43 18.45 55.81

11.84 4.82 3.83 17

6

8 10 14

40.43 31.88 74.05

25.86 8.08 21.94

14.62 6.61 14.66

13

14

18

119.12

44.18

11.64

8 10 14 18

18.66 33.36 56.67 194.04

23.7 32.13 81.42

19.82 6.53 11.09 21.68

8 10 14 18

31.31 108.6 97.02 244.89

19.22 36.06 58.08

26.52 6.45 6.96 16.68

[ifn]*df /2 178.543 151.776 266.691 808.695 373.120 626.167

/10 89.271 75.888 133.346 404.348 186.560 313.083

8.927 7.589 13.335 40.435 18.656 31.308

[ifn]*df /2 /10 354.003 177.002 17.700 611.191 305.595 30.560 546.868 273.434 27.343 401.402 200.701 20.070 637.517 318.758 31.876 667.101 333.550 33.355 2171.985 1085.992 108.599 [ifn]*df /2 186.821 190.033 253.667 1183.056 1480.944 1133.470 1940.335

/10 93.41 95.017 126.833 591.528 740.472 566.735 970.168

9.34 9.502 12.683 59.153 74.047 56.673 97.017

[ifn]*df /2 /10 159.705 79.852 7.985 192.080 96.040 9.604

332.744 166.372 2965.733 1482.867 2382.423 1191.212 3880.888 1940.444 4897.844 2448.92

16.637 148.287 119.121 194.044 244.89

[ifn]*df /2 46.206 131.981 727.199 517.133

23.103 65.991 363.600 258.567

/10 2.310 6.599 36.360 25.857

182.591 309.672 235.433 308.556 161.565 473.967 384.470

91.295 154.836 117.716 154.278 80.783 236.983 192.235

9.130 15.484 11.772 15.428 8.078 23.698 19.223

69.836 436.196 688.498 369.027 438.800 642.540 721.245

34.918 218.098 344.249 184.513 219.400 321.270 360.623

3.492 21.810 34.425 18.451 21.940 32.127 36.062

108.537 560.486 641.052 1116.259 883.679 1628.306 1161.658

54.269 280.243 320.526 558.129 441.840 814.153 580.829

5.427 28.024 32.053 55.813 44.184 81.415 58.083

[ifn]*df /2 /10 -1.979 -0.990 114.659 57.330 236.853 118.426 292.395 146.197

0.000 5.733 11.843 14.620

396.338 530.433

198.169 265.217

19.817 26.522

71.813 9.129 86.095 96.410 132.116 130.529 128.942

35.906 4.565 43.047 48.205 66.058 65.264 64.471

3.591 0.456 4.305 4.820 6.606 6.526 6.447

96.410 50.389 117.040 76.573 293.188 221.777 139.257

48.205 25.195 58.520 38.287 146.594 110.888 69.628

4.820 2.519 5.852 3.829 14.659 11.089 6.963

-5.153 46.422 13.096 340.003 232.885 433.631 333.655

-2.577 23.211 6.548 170.001 116.443 216.816 166.827

-0.258 2.321 0.655 17.000 11.644 21.682 16.683

Protein

sample

Pr TC Ibs [protein] [protein] [protein] OD 600 2 0.018 0.034 0.000 3.64 4 0.004 0.051 0.000 3.66 6 0.030 0.065 0.000 7.23 10 0.137 0.214 0.095 11.72 14 0.224 0.289 0.000 13.48 18 0.111 0.324 0.047 11.84

Ind hour 3

4

2 4 6 10 14 18

0.018 0.011 0.032 0.179 0.263 0.162

0.062 0.082 0.117 0.106 0.279 0.304

0.000 0.000 0.000 0.107 0.052 0.000

3.58 5.61 7.62 11.92 14.01 12.8

11

2 4 6 10 14 18

0.014 0.013 0.013 0.066 0.119 0.153

0.067 0.085 0.000 0.219 0.228 0.211

0.000 0.000 0.000 0.000 0.000 0.000

3.34 3.05 5.4 8.04 9.31 9.79

12

2 4 6 10 14 18

0.016 0.006 0.018 0.069 0.079 0.231

0.068 0.087 0.072 0.226 0.220 0.204

0.000 0.000 0.000 0.000 0.000 0.000

3.64 2.89 5.95 7.88 8.43 10.44

7

2 4 6 10 14 18

0.147 0.087 0.081 0.257 0.225 0.259

0.149 0.147 0.162 0.371 0.316 0.200

0.000 0.000 0.000 0.000 0.049 0.000

12.64 13.75 12.15 13.96 16.05 12.66

8

2 4 6 10 14 18

0.119 0.084 0.094 0.265 0.267 0.150

0.182 0.135 0.177 0.317 0.328 0.162

0.000 0.000 0.000 0.050 0.049 0.000

12.8 12.51 14.67 13.98 15.47 13.25

15

2 4 6 10

0.103 0.060 0.100 0.202

0.190 0.107 0.111 0.350

0.000 0.000 0.000 0.000

11.91 13.01 13.53 13.22

16

14 18

0.155 0.145

0.292 0.194

0.000 0.000

13.04 13.13

2 4 6 10 14 18

0.140 0.078 0.096 0.201 0.209 0.121

0.157 0.148 0.142 0.355 0.315 0.199

0.000 0.000 0.000 0.047 0.000 0.000

12.52 11.29 13.64 12.49 13.09 12.8

av blank 0.1 0.2 0.3 0.4 0.5 bradford run 200209 pr 17 14h 18 14h 17 18h 18 18h tc 17 14h 18 14h 17 18h 18 18h ib 17 14h 18 14h 17 18h 18 18h pr 22 14h 25 14h 22 18h 25 14h tc 22 14h 25 14h 22 18h 25 14h ib 22 14h 25 14h 22 18h 25 14h

0.36 0.49 0.55 0.75 0.85 0.95

av-bl

0.37 0.49 0.59 0.67 0.86 0.95

0.37 0.48 0.64 0.85 0.81 1.01

0.54 0.55 0.63 0.66

0.54 0.55 0.63 0.66

2 2 2 2

0.54 0.55 0.63 0.66

0.17 0.19 0.27 0.29

std dev %cv 0.000 0.000 0.000 0.000

0.000 0.000 0.000 0.000

0.84 0.9 0.76 0.87

0.84 0.9 0.76 0.87

2 2 2 2

0.84 0.9 0.76 0.87

0.47 0.53 0.4 0.51

0.000 0.000 0.000 0.000

0.000 0.000 0.000 0.000

0.390 0.439 0.326 0.416

0.780 0.877 0.652 0.833

0.86 0.74 0.67 0.88

0.82 0.72 0.65 0.72

1 1 1 1

0.84 0.73 0.66 0.8

0.48 0.36 0.3 0.43

0.029 0.018 0.013 0.116

3.449 2.427 2.025 14.550

0.393 0.301 0.247 0.357

0.393 0.301 0.247 0.357

0.88 0.92 1.03 0.9

0.88 0.92 1.03 0.9

2 2 2 2

0.88 0.92 1.03 0.9

0.52 0.56 0.67 0.54

0.000 0.000 0.000 0.000

0.000 0.000 0.000 0.000

0.427 0.460 0.550 0.441

0.854 0.920 1.100 0.882

0.73 0.74 0.76 0.73

0.73 0.74 0.76 0.73

2 2 2 2

0.73 0.74 0.76 0.73

0.36 0.38 0.4 0.36

0.000 0.000 0.000 0.000

0.000 0.000 0.000 0.000

0.299 0.314 0.328 0.299

0.597 0.627 0.655 0.597

0.54 0.6 0.66 0.71

0.54 0.6 0.66 0.71

2 2 2 2

0.54 0.6 0.66 0.71

0.18 0.24 0.29 0.35

0.000 0.000 0.000 0.000

0.000 0.000 0.000 0.000

0.149 0.194 0.242 0.286

0.297 0.388 0.484 0.573

df

0.36 0.48 0.59 0.76 0.84 0.97

av

0 0.12 0.23 0.39 0.47 0.6

av-bl

protein conc[prot]*df 0.143 0.286 0.157 0.314 0.222 0.444 0.243 0.485

actual [prot] 0.143 0.157 0.222 0.243 0.390 0.439 0.326 0.416 0.197 0.150 0.124 0.179 0.427 0.460 0.550 0.441 0.299 0.314 0.328 0.299 0.149 0.194 0.242 0.286

pr 17 14h 18 14h 17 18h 18 18h tc 17 14h 18 14h 17 18h 18 18h ib 17 14h 18 14h 17 18h 18 18h pr 22 14h 25 14h 22 18h 25 14h tc 22 14h 25 14h 22 18h 25 14h ib 22 14h 25 14h 22 18h 25 14h

actual [prot] 0.14 0.16 0.22 0.24 0.39 0.44 0.33 0.42 0.2 0.15 0.12 0.18 0.43 0.46 0.55 0.44 0.3 0.31 0.33 0.3 0.15 0.19 0.24 0.29

bradford sample 25oC

warning!! All sample name are change to 23 and 24

std av 0.1 0.2 0.3 0.4 0.5 bl

pr 4h 17 4h 18 6h 17 6h 18 8h 17 8h 18 10h 17 10h 18 14h 17 14h 18 18h 17 18h 18 ib 4h 17 4h 18 6h 17 6h 18 8h 17 8h 18 10h 17 10h 18 14h 17 14h 18 18h 17 18h 18 tc 4h 17 4h 18 6h 17 6h 18 8h 17 8h 18 10h 17 10h 18

0.57 0.79 0.9 1.04 1.11 0.37

0.56 0.76 0.89 1.03 1.18 0.36

0.57 0.76 0.86 1.01 1.13 0.37

0.93 0.96 0.96 0.92 0.75 0.73 0.59 0.6 0.95 0.93 0.94 0.95

0.97 0.99 0.95 0.93 0.75 0.74 0.61 0.6 0.94 0.96 0.95 0.94

df

0.55 0.56 0.92 0.95 0.95

av 2 2 2 2 2 2 2 2 2 2 2 2

df 0.95 0.7 0.71 0.7 0.63 0.68 0.73 0.72 0.72

0.67 0.72 0.71 0.79 0.76 0.76 0.65 0.63 0.72 0.71 0.75 0.73

0.71 0.73 0.71 0.75 0.78 0.78 0.67 0.66 0.7 0.79 0.77 0.72

0.78 0.77 0.78 0.81 0.99 0.71 0.71

0.79 0.78 0.86

0.63 0.85

0.99 0.77 0.66 0.87

0.95 0.98 0.95 0.92 0.75 0.73 0.58 0.58 0.93 0.95 0.94 0.94 av

2 2 2 2 2 2 2 2 2 2 2 2 df

0.75 0.72 0.79 0.84

av-bl 0.56 0.77 0.87 1.03 1.14 0.37

av-blank std dev %cv 0.584 0.031 0.609 0.018 0.588 0.008 0.556 0.009 0.383 0.003 0.369 0.008 0.218 0.032 0.217 0.022 0.569 0.012 0.582 0.017 0.576 0.004 0.578 0.003

3.275 1.886 0.816 0.998 0.378 1.059 5.413 3.765 1.290 1.820 0.450 0.280

protein conc 0.348 0.367 0.350 0.325 0.190 0.178 0.060 0.060 0.336 0.346 0.341 0.343

av-blank std dev %cv protein conc 0.78 0.410 0.152 19.622 0.211 0.72 0.351 0.015 2.024 0.164 0.71 0.348 0.001 0.099 0.162 0.75 0.382 0.040 5.285 0.189 0.75 0.381 0.042 5.646 0.188 0.77 0.404 0.020 2.571 0.206 0.65 0.282 0.023 3.495 0.110 0.65 0.280 0.016 2.520 0.108 0.7 0.330 0.021 2.944 0.148 0.74 0.377 0.040 5.422 0.185 0.75 0.380 0.028 3.758 0.187 0.72 0.357 0.010 1.365 0.169

av 4 4 4 4 4 4 4 2

0.4 0.51 0.66 0.78 0

0.77 0.75 0.81 0.82 0.99 0.74 0.67 0.86

av-blank std dev %cv 0.405 0.020 0.388 0.030 0.445 0.043 0.457 0.017 0.625 0.003 0.374 0.040 0.301 0.039 0.491 0.016

2.633 4.009 5.283 2.062 0.285 5.351 5.827 1.899

protein conc 0.207 0.194 0.238 0.248 0.380 0.183 0.126 0.274

14h 17 14h 18 18h 17 18h 18

0.75 0.72 0.68 0.76

0.79 0.93 0.9 0.9

0.87 0.91 0.8

4 4 4 4

0.77 0.84 0.83 0.82

0.401 0.472 0.467 0.456

0.030 0.107 0.131 0.072

3.967 12.709 15.682 8.780

0.203 0.260 0.255 0.247

[prot]*df actual [prot] 0.695 0.348 0.734 0.367 0.701 0.350 0.650 0.325 0.379 0.190 0.357 0.178 0.121 0.060 0.119 0.060 0.671 0.336 0.692 0.346 0.683 0.341 0.686 0.343 [prot]*df actual [prot] 0.422 0.211 0.329 0.164 0.324 0.162 0.378 0.189 0.377 0.188 0.412 0.206 0.221 0.110 0.217 0.108 0.297 0.148 0.370 0.185 0.374 0.187 0.338 0.169 [prot]*df actual [prot] 0.829 0.415 0.776 0.388 0.953 0.476 0.991 0.496 1.519 0.760 0.731 0.365 0.502 0.251 0.548 0.274

sample

pr

tc

ib

17

8 10 14 18

0.190 0.060 0.336 0.341

0.760 0.251 0.407 0.511

0.188 0.110 0.148 0.187

18

8 10 14 18

0.178 0.060 0.346 0.343

0.365 0.274 0.519 0.494

0.206 0.108 0.185 0.169

0.814 1.038 1.022 0.987

0.407 0.519 0.511 0.494

Protein

20

sample

Pr TC Ibs [protein] [protein] [protein] OD 600 8 0.029 0.060 0.043 3.835 10 0.041 0.091 0.016 2.185 14 0.018 0.109 0.105 4.637 18 0.045 0.142 0.081 5.530

Ind hour 1

2

8 10 14 18

9

8 10 14 18

0.031 0.007 0.109 0.055

3.245 2.474 3.601 4.263

10

8 10 14 18

0.112 0.144 0.134

0.008 0.052 0.042

3.397 2.235 3.483 4.418

0.027 0.009 0.045

8 10 14 18

0.090 0.197 0.143 0.226

0.149 0.141 0.181 0.168

0.123 0.099 0.042 0.148

14.254 13.339 13.323 12.688

8 10 14 18

0.144 0.174 0.224 0.294

0.278 0.171 0.221 0.213

0.190 0.087 0.038 0.140

15.622 13.339 14.487 12.676

13

8 10 14 18

0.070 0.162 0.105 0.329

0.138 0.252 0.215 0.225

0.128 0.052 0.089 0.092

15.230 13.767 11.692 12.446

14

8 10 14 18

0.079 0.197 0.163 0.286

0.140 0.130 0.189 0.116

0.179 0.106 0.127 0.082

14.482 13.767 11.631 13.296

5

6

0.015 0.028 0.017 0.042

0.054 0.055 0.111 0.106

Pr [protein] 2 0.018 4 0.004 6 0.030 10 0.137 14 0.224 18 0.111

Ind hour 3

4

2 4 6 10 14 18

0.018 0.011 0.032 0.179 0.263 0.162

11

2 4 6 10 14 18

0.014 0.013 0.013 0.066 0.119 0.153

12

2 4 6 10 14 18

0.016 0.006 0.018 0.069 0.079 0.231

7

2 4 6 10 14 18

0.147 0.087 0.081 0.257 0.225 0.259

8

2 4 6 10 14 18

0.119 0.084 0.094 0.265 0.267 0.150

15

2 4 6 10

0.103 0.060 0.100 0.202

16

14 18

0.155 0.145

2 4 6 10 14 18

0.140 0.078 0.096 0.201 0.209 0.121

30 TC Ibs [protein] [protein] OD 600 0.034 0.000 3.64 0.051 0.000 3.66 0.065 0.000 7.23 0.214 0.095 11.72 0.289 0.000 13.48 0.324 0.047 11.84 0.062 0.082 0.117 0.106 0.279 0.304

0.000 0.000 0.000 0.107 0.052 0.000

3.58 5.61 7.62 11.92 14.01 12.8

0.067 0.085 0.000 0.219 0.228 0.211

0.000 0.000 0.000 0.000 0.000 0.000

3.34 3.05 5.4 8.04 9.31 9.79

0.068 0.087 0.072 0.226 0.220 0.204

0.000 0.000 0.000 0.000 0.000 0.000

3.64 2.89 5.95 7.88 8.43 10.44

0.149 0.147 0.162 0.371 0.316 0.200

0.000 0.000 0.000 0.000 0.049 0.000

12.64 13.75 12.15 13.96 16.05 12.66

0.182 0.135 0.177 0.317 0.328 0.162

0.000 0.000 0.000 0.050 0.049 0.000

12.8 12.51 14.67 13.98 15.47 13.25

0.190 0.107 0.111 0.350

0.000 0.000 0.000 0.000

11.91 13.01 13.53 13.22

0.292 0.194

0.000 0.000

13.04 13.13

0.157 0.148 0.142 0.355 0.315 0.199

0.000 0.000 0.000 0.047 0.000 0.000

12.52 11.29 13.64 12.49 13.09 12.8

9

X dcw

Y Av (OD) 0.75 1.61 1.6 3.29 2.65 4.78 3.4 6.46 4.7 8.03

f(x) = 1.64x + 0.54 R² = 0.99

8 7 6 5 4 3 2 1 0 0.5

30oC sample

1

1.5

t (induce) OD 600 DCW 3 2 3.64 1.89 4 3.66 1.9 6 7.23 4.08 10 11.72 6.82 14 13.48 7.9 18 11.84 6.9 4

2 4 6 10 14 18

3.58 5.61 7.62 11.92 14.01 12.8

1.85 3.09 4.32 6.95 8.22 7.49

2 4 6 10 14 18

3.34 3.05 5.4 8.04 9.31 9.79

1.7 1.53 2.97 4.58 5.35 5.65

2 4 6 10 14 18

3.64 2.89 5.95 7.88 8.43 10.44

1.89 1.43 3.3 4.48 4.82 6.05

7

2 4 6 10 14 18

12.64 13.75 12.15 13.96 16.05 12.66

7.39 8.07 7.09 8.2 9.47 7.4

8

2 4 6 10

12.8 12.51 14.67 13.98

7.49 7.31 8.63 8.2

11

12

2

20oC samplet 1

sample t 9

sample t 10

sample t 5

2.5

3

3.5

induce 1 2 3 4 10 12 14 18 22 26

av

induce

induce

-0.2 -0.02 0.06 0.61 1.33 2.01 1.18 2.5 3.05 3.28 dcw

0.12 0.51 0.63 1.58 2.21 3.24 2.47 3.6 4.26 4.71 av

-0.26 -0.02 0.05 0.63 1.02 1.65 1.18 1.87 2.27 2.55 dcw

0.12 0.47 0.58 1.48 2.21 3.4 3.34 3.48 4.42 4.29

0 6 8 10 14 18 22 induce

1 2 4

dcw

av

0 6 8 10 14 18 22

1 2 3 4 10 12 14 18 22 26

4.5

0.21 0.51 0.65 1.55 2.72 3.84 2.47 4.64 5.53 5.91

0 6 8 10 14 18 22

1 2 3 4 10 12 14 18 22 26

4

av OD

-0.26 -0.04 0.02 0.57 1.02 1.74 1.7 1.79 2.37 2.29 dcw

0.27 0.62 1.86

-0.17 0.05 0.8

5

14 18

15.47 13.25

9.12 7.76

15

2 4 6 10 14 18

11.91 13.01 13.53 13.22 13.04 13.13

6.94 7.61 7.93 7.75 7.63 7.69

16

2 4 6 10 14 18

12.52 11.29 13.64 12.49 13.09 12.8

7.31 6.56 8 7.3 7.67 7.49

17

0 14 18

4.49 8.69 9.31

2.41 4.98 5.35

18

0 14 18

4.3 11.79 11.03

2.29 6.87 6.41

6 7 9 13 15 17 21 25 29 sample t 6

sample t 13

sample t 14

0 2 6 8 10 14 18 22 induce

1 2 4 6 7 9 13 15 17 21 25 29

av

induce

dcw

av

dcw

av

0 2 6 8 10 14 18 22

-0.17 0.05 1.02 2.92 4.4 6.03 6.92 9.21 8.52 7.82 7.41 7.09

0.26 0.61 2.02 5.39 6.84 7.73 9.48 15.23 9.56 11.69 12.45 13.71

0 2 6 8 10 14 18 22 induce

2.69 3.53 4.77 5.59 8.37 6.34 7.81 7.42 7.09

0.27 0.62 2.22 5.33 7.74 10.42 11.87 15.62 14.49 13.34 12.68 12.15

0 2 6 8 10 14 18 22

1 2 4 6 7 9 13 15 17 21 25 29

1 2 4 6 7 9 13 15 17 21 25 29

4.95 6.33 8.36 9.7 14.25 10.92 13.32 12.69 12.15

-0.18 0.04 0.9 2.96 3.85 4.39 5.46 8.97 5.51 6.81 7.27 8.04 dcw

0.26 0.61 2.69 5.57 8.26 9.23 11.07 14.48 13.77 11.63 13.3 13.19

-0.18 0.04 1.31 3.07 4.72 5.31 6.43 8.51 8.08 6.77 7.79 7.73

4

25oC sample

t 23

4.5

5

24

induce 0 1 3 4 5 7 10 11 13 15 19 23 27 29 0 1 3 4 5 7 10 11 13 15 19 23 27 29

sample

av OD

dcw

0 2 5 6 8 10 14 18 22 24

0.2 0.36 2.43 3.18 3.99 5.97 8.28 10.85 11.19 12.34 13.47 12.73 11.38 10.82

-0.21 -0.11 1.15 1.61 2.1 3.31 4.73 6.29 6.5 7.21 7.9 7.44 6.62 6.28

0 2 5 6 8 10 14 18 22 24

0.2 0.37 1.73 3.38 4.11 6.13 8.55 11.32 11.34 13.26 15.41 11.75 11.33 10.91

-0.21 -0.11 0.72 1.73 2.18 3.41 4.89 6.58 6.59 7.77 9.08 6.84 6.59 6.33

OD 600 DCW 13.142 7.695 11.798 6.874

20

14 18

21

14 18

13.840 11.200

8.122 6.509

22

14 18

14.610 11.822

8.592 6.889

RSM (inoculum problem) 22 t od 0 8 10 14

repeat 4.82 8.83 17.87 9.63

DCW 2.610 5.063 10.586 5.550

22 t

od 14 18

25,26,27,28t

dcw 13.34 15.49

od

7.816 9.130 dcw

18

7.77

4.415

25

0 8 10 12 14 16 18

4.28 9.57 13.64 16.82 9.47 14.32 7.83

2.279 5.515 8.000 9.942 5.450 8.415 4.450

26

0 8 10 12 14 16 18

4.42 9.43 17.18 17.54 11.05 9.65 7.34

2.365 5.425 10.163 10.384 6.420 5.565 4.154

27

0 8 10 12 14 16 18

4.41 9.01 17.28 12.19 12.43 9.01 7.62

2.359 5.170 10.221 7.111 7.262 5.172 4.321

28

0 8 10 12 14 16 18

4.61 13.93 14.64 10.01 12.95 8.84 9.11

2.484 8.175 8.608 5.783 7.581 5.065 5.234

14 18

14.36 15.42

8.437 9.088

30oC

mg/mL ng/mL Pr TC Ibs Pr TC Ibs sample OD 600 DCW Ind hour[protein] [protein] [protein] [IFN] [IFN] [IFN] 3 3.64 1.89 2 0.02 0.03 0 2.31 3.63 3.66 1.9 4 0 0.05 0 0.68 5.53 7.23 4.08 6 0.03 0.07 0 5.37 7.59 11.72 6.82 10 0.14 0.21 0.09 12.73 9.88 13.48 7.9 14 0.22 0.29 0 39.54 12.22 11.84 6.9 18 0.11 0.32 0.05 13.85 11.16 4

11

12

7

8

15

1.44 0.46 4.56 7.15 6.01

3.58 5.61 7.62 11.92 14.01 12.8

1.85 3.09 4.32 6.95 8.22 7.49

2 4 6 10 14 18

0.02 0.01 0.03 0.18 0.26 0.16

0.06 0.08 0.12 0.11 0.28 0.3

0 0 0 0.11 0.05 0

2.93 2.21 5.26 22.21 60.64 28.53

3.21 5.16 6.03 10.71 18.85 14.88

1.33 1.28 4.11

3.34 3.05 5.4 8.04 9.31 9.79

1.7 1.53 2.97 4.58 5.35 5.65

2 4 6 10 14 18

0.01 0.01 0.01 0.07 0.12 0.15

0.07 0.08 0 0.22 0.23 0.21

0 0 0 0 0 0

2.83 2.56 2.68 11.82 13.67 12.43

3.76 11.21 0.22 16.16 20.55 18.64

0.88 3.15 10.77

3.64 2.89 5.95 7.88 8.43 10.44

1.89 1.43 3.3 4.48 4.82 6.05

2 4 6 10 14 18

0.02 0.01 0.02 0.07 0.08 0.23

0.07 0.09 0.07 0.23 0.22 0.2

0 0 0 0 0 0

4.2 4.52 3.41 14.93 14.54 21.07

5.24 11.64 11.63 18.67 22.38 21.57

2.18 7.36 6.69

12.64 13.75 12.15 13.96 16.05 12.66

7.39 8.07 7.09 8.2 9.47 7.4

2 4 6 10 14 18

0.15 0.09 0.08 0.26 0.22 0.26

0.15 0.15 0.16 0.37 0.32 0.2

0 0 0 0 0.05 0

12.14 18.43 15.33 43.83 29.65 27.34

6.03 10.91 9.44 15.14 17.63 15.35

18.02 5.93 4.66

12.8 12.51 14.67 13.98 15.47 13.25

7.49 7.31 8.63 8.2 9.12 7.76

2 4 6 10 14 18

0.12 0.08 0.09 0.27 0.27 0.15

0.18 0.14 0.18 0.32 0.33 0.16

0 0 0 0.05 0.05 0

17.44 17.03 24.44 34.28 29.56 12.85

6.84 12.71 9.15 15.18 16.72 14.51

17.15 6.49 5.89

11.91 13.01 13.53 13.22 13.04 13.13

6.94 7.61 7.93 7.75 7.63 7.69

2 4 6 10 14 18

0.1 0.06 0.1 0.2 0.16 0.14

0.19 0.11 0.11 0.35 0.29 0.19

0 0 0 0 0 0

12.71 20.42 17.29 23.95 27.85 11.65

7.4 13.92 12.07 20.9 18.76 20.39

9.93 5.38 7.39

12.72 8.87

16.43 8.26

9.5 12.07

15.8 8.55

11.21 7.02

15.82 7.65

16

12.52 11.29 13.64 12.49 13.09 12.8

7.31 6.56 8 7.3 7.67 7.49

2 4 6 10 14 18

0.14 0.08 0.1 0.2 0.21 0.12

0.16 0.15 0.14 0.35 0.32 0.2

0 0 0 0.05 0 0

12.73 17.4 16.7 25.39 21.25 12.7

mg/mL ng/mL Pr TC Ibs Pr TC sample OD 600 DCW Ind hour[protein] [protein] [protein] [IFN] [IFN] 1 3.84 2.01 8 0.03 0.06 0.04 8.93 2.47 1.18 10 0.04 0.09 0.02 17.7 4.64 2.5 14 0.02 0.11 0.1 9.34 5.53 3.05 18 0.05 0.14 0.08 7.99

11.47 14.87 13.73 24.03 33.89 23.6

6.72 6.25 7.76 18.67 9.63

20oC

Ibs [IFN] 2.31 9.13 3.49 5.43

0 3.59 4.82 0

9

3.24 2.47 3.6 4.26

1.65 1.18 1.87 2.27

8 10 14 18

0.02 0.03 0.02 0.04

0.05 0.05 0.11 0.11

0.03 0.01 0.11 0.06

7.59 30.56 9.5 9.6

6.6 15.48 21.81 28.02

5.73 0.46 2.52 2.32

10

3.4 3.34 3.48 4.42

1.74 1.7 1.79 2.37

8 10 14 18

0.03 0.01 0.05

0.11 0.14 0.13

0.01 0.05 0.04

27.34 12.68 16.64

11.77 34.42 32.05

4.3 5.85 0.65

5

14.25 10.92 13.32 12.69

8.37 6.34 7.81 7.42

8 10 14 18

0.09 0.2 0.14 0.23

0.15 0.14 0.18 0.17

0.12 0.1 0.04 0.15

13.33 20.07 59.15 148.29

36.36 15.43 18.45 55.81

11.84 4.82 3.83 17

6

15.62 14.49 13.34 12.68

9.21 8.52 7.82 7.41

8 10 14 18

0.14 0.17 0.22 0.29

0.28 0.17 0.22 0.21

0.19 0.09 0.04 0.14

40.43 31.88 74.05 119.12

25.86 8.08 21.94 44.18

14.62 6.61 14.66 11.64

13

15.23 9.56 11.69 12.45

8.97 5.51 6.81 7.27

8 10 14 18

0.07 0.16 0.1 0.33

0.14 0.25 0.21 0.22

0.13 0.05 0.09 0.09

18.66 33.36 56.67 194.04

23.7 32.13 81.42

19.82 6.53 11.09 21.68

14.48 13.77 11.63 13.3

8.51 8.08 6.77 7.79

8 10 14 18

0.08 0.2 0.16 0.29

0.14 0.13 0.19 0.12

0.18 0.11 0.13 0.08

31.31 108.6 97.02 244.89

19.22 36.06 58.08

26.52 6.45 6.96 16.68

14

25oC

mg/mL ng/mL Pr TC Ibs Pr TC Ibs sample OD 600 DCW Ind hour[protein] [protein] [protein] [IFN] [IFN] [IFN] 23 11.19 6.5 8 0.19 0.76 0.19 240.08 235.74 190.61 12.34 7.21 10 0.06 0.25 0.11 83.42 97.74 53.58 13.47 7.9 14 0.34 0.41 0.15 361.21 353.76 371.8 12.73 7.44 18 0.34 0.51 0.19 343.02 331.58 371.08 24

11.34 13.26 15.41 11.75

6.59 7.77 9.08 6.84

8 10 14 18

0.18 0.06 0.35 0.34

0.37 0.27 0.52 0.49

0.21 0.11 0.18 0.17

218.05 68.1 340.94 340.62

273.37 91.31 299.32 322.65

164.7 50.25 349.86 389.9

RSM duplicate 25oC

mg/mL ng/mL Pr TC Ibs Pr TC Ibs sample OD 600 DCW Ind hour[protein] [protein] [protein] [IFN] [IFN] [IFN] 20 13.14 7.9 14 0.34 0.41 0.15 361.21 353.76 371.8 11.8 7.44 18 0.34 0.51 0.19 343.02 331.58 371.08 21

13.84 11.2

7.9 7.44

14 18

0.34 0.34

0.41 0.51

0.15 0.19

361.21 343.02

353.76 331.58

371.8 371.08

(2.66 iptg)22 14.61 11.82

7.9 7.44

14 18

0.34 0.34

0.41 0.51

0.15 0.19

361.21 343.02

353.76 331.58

371.8 371.08

22

13.34 15.49

7.82 9.13

14 18

0.43 0.55

0.3 0.33

0.15 0.24

191.37 191.81

159.35 130.98

-0.58 5.61

25

14.36 15.42

8.44 9.09

14 18

0.46 0.44

0.31 0.3

0.19 0.29

180.4 193.42

129.07 131.46

0.62 6.17

26

14.36 15.42

8.44 9.09

14 18

0.46 0.44

0.31 0.3

0.19 0.29

172.78 202.22

120.68 175.93

4.23 4.98

17

8.69 9.31

4.98 5.35

14 18

0.14 0.22

0.39 0.33

0.2 0.12

18

11.79 11.03

6.87 6.41

14 18

0.16 0.24

0.44 0.42

0.15 0.18

14h 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

0.02 0.02 0.22 0.26 0.14 0.22 0.22 0.27 0.02 0.01 0.12 0.08 0.1 0.16 0.16 0.21 0.14 0.16 0 0.34 0.34 0.43 0.34 0.35 0.46 0.46 0.46 0.46

0.11 0.11 0.29 0.28 0.18 0.22 0.32 0.33 0.11 0.14 0.23 0.22 0.21 0.19 0.29 0.32 0.39 0.44 0 0.41 0.41 0.3 0.41 0.52 0.31 0.31 0.31 0.31

0.1 0.1 0 0.05 0.04 0.04 0.05 0.05 0.11 0.05 0 0 0.09 0.13 0 0 0.2 0.15 0 0.15 0.15 0.15 0.15 0.18 0.19 0.19 0.19 0.19

18h pr 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

tc 0.05 0.05 0.11 0.16 0.23 0.29 0.26 0.15 0.04 0.05 0.15 0.23 0.33 0.29 0.14 0.12 0.22 0.24 0 0.34 0.34 0.55 0.34 0.34 0.44 0.44 0.44 0.44

ib 0.14 0.14 0.32 0.3 0.17 0.21 0.2 0.16 0.11 0.13 0.21 0.2 0.22 0.12 0.19 0.2 0.33 0.42 0 0.51 0.51 0.33 0.51 0.49 0.3 0.3 0.3 0.3

0.08 0.08 0.05 0 0.15 0.14 0 0 0.06 0.04 0 0 0.09 0.08 0 0 0.12 0.18 0 0.19 0.19 0.24 0.19 0.17 0.29 0.29 0.29 0.29

rpt (200209)actual [ifn] 26 14h pr 172.78 25 14h pr 180.4 22 14h pr 191.37 26 18h pr 202.22 25 18h pr 193.42 22 18h pr 191.81 26 14h tc 120.68 25 14h tc 129.07 22 14h tc 159.35 26 18h tc 175.93 25 18h tc 131.46 22 18h tc 130.98 /10 actual [ifn] 22 14h ib 25 14h ib 26 14h ib 22 18h ib 25 18h ib 26 18h ib

interferon

2 hour

DCW (g/L) Pr 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

TC

IB

1.89 1.85

2.31 2.93

3.63 3.21

7.39 7.49

12.14 17.44

6.03 6.84

1.7 1.89

2.83 4.2

3.76 5.24

6.94 7.31

12.71 12.73

7.4 11.47

Pr + TC 0.000 0.000 1.44 5.942 1.33 6.146 0.000 0.000 18.02 18.175 17.15 24.278 0.000 0.000 0.88 6.593 2.18 9.440 0.000 0.000 9.93 20.110 6.72 24.204 0.000 0.000

% transfer soluble Pr/(Pr+TC)*100 0.000 #DIV/0! 0.000 #DIV/0! 7.377 38.883 7.476 47.730 0.000 #DIV/0! 0.000 #DIV/0! 36.194 66.815 41.424 71.823 0.000 #DIV/0! 0.000 #DIV/0! 7.472 42.944 11.615 44.481 0.000 #DIV/0! 0.000 #DIV/0! 30.035 63.207 30.929 52.594 0.000 #DIV/0! 0.000 #DIV/0!

total (Pr+TC+IB)

interferon

4 hour

DCW (g/L) Pr 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

TC

IB

1.9 3.09

0.68 2.21

5.53 5.16

8.07 7.31

18.43 17.03

10.91 12.71

1.53 1.43

2.56 4.52

11.21 11.64

7.61 6.56

20.42 17.4

13.92 14.87

Pr + TC 0.000 0.000 0.46 6.211 1.28 7.365 0.000 0.000 5.93 29.339 6.49 29.739 0.000 0.000 3.15 13.775 7.36 16.157 0.000 0.000 5.38 34.344 6.25 32.270 0.000 0.000

% transfer soluble Pr/(Pr+TC)*100 0.000 #DIV/0! 0.000 #DIV/0! 6.668 10.983 8.641 29.979 0.000 #DIV/0! 0.000 #DIV/0! 35.270 62.826 36.226 57.258 0.000 #DIV/0! 0.000 #DIV/0! 16.929 18.595 23.516 27.946 0.000 #DIV/0! 0.000 #DIV/0! 39.720 59.467 38.518 53.921 0.000 #DIV/0! 0.000 #DIV/0!

total (Pr+TC+IB)

interferon

6 hour

DCW (g/L) Pr 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

TC

IB

4.08 4.32

5.37 5.26

7.59 6.03

7.09 8.63

15.33 24.44

9.44 9.15

2.97 3.3

2.68 3.41

0.22 11.63

7.93 8

17.29 16.7

12.07 13.73

Pr + TC 0.000 0.000 4.56 12.956 4.11 11.291 0.000 0.000 4.66 24.771 5.89 33.591 0.000 0.000 10.77 2.892 6.69 15.040 0.000 0.000 7.39 29.358 7.76 30.437 0.000 0.000

% transfer soluble Pr/(Pr+TC)*100 0.000 #DIV/0! 0.000 #DIV/0! 17.512 41.455 15.397 46.581 0.000 #DIV/0! 0.000 #DIV/0! 29.433 61.905 39.482 72.766 0.000 #DIV/0! 0.000 #DIV/0! 13.664 92.495 21.725 22.660 0.000 #DIV/0! 0.000 #DIV/0! 36.744 58.889 38.193 54.875 0.000 #DIV/0! 0.000 #DIV/0!

total (Pr+TC+IB)

interferon

8 hour

DCW (g/L) Pr

TC

IB

Pr + TC

total (Pr+TC+IB)

% transfer soluble Pr/(Pr+TC)*100

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

interferon % transfer

10 hour 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

DCW (g/L) Pr TC 1.18 17.700 1.5 21.458 6.82 12.73 6.95 22.21 6.34 20.07 8.52 31.876 8.2 43.83 8.2 34.28 1.18 30.560 1.71 27.343 4.58 11.82 4.48 14.93 5.51 33.355 8.08 108.599 7.75 23.95 7.3 25.39 7.21 83.42 7.77 68.1

IB 9.130 11.798 9.88 10.71 15.43 8.078 15.14 15.18 15.484 11.772 16.16 18.67 23.698 19.223 20.9 24.03 97.74 91.31

total soluble Pr + TC (Pr+TC+IB) Pr/(Pr+TC)*100 3.591 26.830 30.421 65.971 6.234 33.256 39.490 64.524 0.000 22.613 22.613 56.295 0.000 32.921 32.921 67.464 4.820 35.498 40.318 56.538 6.606 39.954 46.560 79.782 0.000 58.970 58.970 74.327 0.000 49.453 49.453 69.311 0.456 46.044 46.500 66.371 4.305 39.115 43.420 69.904 0.000 27.981 27.981 42.237 0.000 33.600 33.600 44.421 6.526 57.053 63.579 58.463 6.447 127.822 134.269 84.961 0.000 44.848 44.848 53.402 0.000 49.425 49.425 51.374 53.58 181.158 234.737 46.046 50.25 159.416 209.662 42.720

interferon % transfer total soluble 14 hour DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB) Pr/(Pr+TC)*100 1 2.5 9.341 3.492 4.820 12.833 17.653 72.789 2 2.3 7.453 1.564 3.760 9.017 12.777 82.655 3 7.9 39.54 12.22 7.15 51.760 58.908 76.394 4 8.22 60.64 18.85 12.72 79.493 92.208 76.285 5 7.81 59.15 18.45 3.83 77.604 81.433 76.224 6 7.82 74.047 21.940 14.659 95.987 110.646 77.143 7 9.47 29.65 17.63 15.8 47.285 63.082 62.710 8 9.12 29.56 16.72 11.21 46.280 57.488 63.870 9 1.87 9.502 21.810 2.519 31.312 33.831 30.346 10 1.8 12.653 34.425 5.852 47.078 52.930 26.877 11 5.35 13.67 20.55 16.43 34.223 50.655 39.942 12 4.82 14.54 22.38 9.5 36.921 46.423 39.392 13 6.81 56.673 32.127 11.089 88.800 99.889 63.821 14 6.77 97.017 36.062 6.963 133.079 140.042 72.902 15 7.63 27.85 18.76 15.82 46.606 62.429 59.751

16 17 18 19 20 21 22 23 24 25 26 27 28

7.67 7.9 9.08

21.25 361.21 340.94

33.89 353.76 299.32

18.67 371.8 349.86

55.137 714.966 640.261

73.803 1086.763 990.118

38.543 50.521 53.250

interferon % transfer total soluble 18 hour DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB) Pr/(Pr+TC)*100 1 3.05 7.985 5.427 0.000 13.412 13.412 59.536 2 3.25 9.985 7.427 1.078 17.412 18.490 57.346 3 6.9 13.85 11.16 6.01 25.004 31.015 55.376 4 7.49 28.53 14.88 8.87 43.406 52.273 65.721 5 7.42 148.287 55.813 17.000 204.100 221.100 72.654 6 7.41 119.121 44.184 11.644 163.305 174.949 72.944 7 7.4 27.34 15.35 8.55 42.689 51.239 64.035 8 7.76 12.85 14.51 7.02 27.358 34.373 46.973 9 2.27 9.604 28.024 2.321 37.628 39.949 25.524 10 2.37 16.637 32.053 0.655 48.690 49.345 34.169 11 5.65 12.43 18.64 8.26 31.070 39.328 40.014 12 6.05 21.07 21.57 12.07 42.643 54.710 49.422 13 7.27 194.044 81.415 21.682 275.459 297.141 70.444 14 7.79 244.892 58.083 16.683 302.975 319.658 80.829 15 7.69 11.65 20.39 7.65 32.037 39.688 36.366 16 7.49 12.7 23.6 9.63 36.298 45.932 34.994 17 7.44 343.02 331.58 371.08 674.599 1045.681 50.848 18 6.84 340.62 322.65 389.9 663.268 1053.171 51.355

blank 0.1 0.2 0.3 0.4

0.37 0.49 0.59 0.67 0.86

0.37 0.48 0.64 0.85 0.81

0.36 0.49 0.55 0.75 0.85

0.5

0.95

1.01

0.95

% transfer all protein (Pr/total)*100 tempt #DIV/0! #DIV/0! 31.319 39.243 #DIV/0! #DIV/0! 33.552 42.095 #DIV/0! #DIV/0! 37.889 36.149 #DIV/0! #DIV/0! 42.321 41.159 #DIV/0! #DIV/0!

20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 25 25

% transfer all protein (Pr/total)*100 tempt #DIV/0! #DIV/0! 10.232 25.551 #DIV/0! #DIV/0! 52.261 47.005 #DIV/0! #DIV/0! 15.131 19.200 #DIV/0! #DIV/0! 51.419 45.173 #DIV/0! #DIV/0!

20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 25 25

IPTG 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2 1.03 1.03

OD induce 1 1 7 7 1 1 7 7 1 1 7 7 1 1 7 7 4 4

0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2 1.03 1.03

OD induce 1 1 1 1 7 7 7 7 1 1 1 1 7 7 7 7 4 4

IPTG

tempt 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

IPTG 20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 25 25

0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2 1.03 1.03

OD induce 1 1 7 7 1 1 7 7 1 1 7 7 1 1 7 7 4 4

% transfer all protein (Pr/total)*100 tempt #DIV/0! #DIV/0! 30.670 34.158 #DIV/0! #DIV/0! 52.100 61.908 #DIV/0! #DIV/0! 19.579 15.687 #DIV/0! #DIV/0! 47.051 43.731 #DIV/0! #DIV/0!

IPTG 20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 25 25

% transfer all protein (Pr/total)*100 tempt

IPTG 20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 25 25

% transfer

0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2 1.03 1.03

OD induce 1 1 1 1 7 7 7 7 1 1 1 1 7 7 7 7 4 4

OD induce 0.05 1 0.05 1 0.05 1 0.05 1 0.05 7 0.05 7 0.05 7 0.05 7 2 1 2 1 2 1 2 1 2 7 2 7 2 7 2 7 1.03 4 1.03 4

all protein (Pr/total)*100 tempt 58.183 54.338 56.295 67.464 49.779 68.462 74.327 69.311 65.720 62.973 42.237 44.421 52.462 80.882 53.402 51.374 35.536 32.482

20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 25 25

% transfer all protein (Pr/total)*100 tempt 52.915 58.331 67.125 65.766 72.640 66.922 47.006 51.418 28.087 23.905 26.986 31.329 56.736 69.277 44.607

20 20 30 30 20 20 30 30 20 20 30 30 20 20 30

IPTG 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2 1.03 1.03

OD induce 1 1 1 1 7 7 7 7 1 1 1 1 7 7 7 7 4 4

0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2

OD induce 1 1 1 1 7 7 7 7 1 1 1 1 7 7 7

IPTG

28.795 33.237 34.434

% transfer all protein (Pr/total)*100 tempt 59.536 54.002 44.644 54.573 67.068 68.089 53.350 37.386 24.041 33.716 31.611 38.521 65.304 76.611 29.356 27.654 32.804 32.342

30 25 25

2 1.03 1.03

7 4 4

0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2 1.03 1.03

OD induce 1 1 1 1 7 7 7 7 1 1 1 1 7 7 7 7 4 4

IPTG 20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 25 25

interferon

10 hour 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

OD 2.47 3 11.72 11.92 10.92 14.49 13.96 13.98 2.47 3.34 8.04 7.88 9.56 13.77 13.22 12.49 12.34 13.26

DCW (g/L) Pr 1.179 1.500 6.824 6.947 6.337 8.517 8.196 8.204 1.179 1.705 4.579 4.480 5.510 8.077 7.745 7.297 7.205 7.766

TC 17.700 21.458 12.730 22.210 20.070 31.876 43.830 34.276 30.560 27.343 11.818 14.925 33.355 108.599 23.950 25.392 83.416 68.102

IB 9.130 11.798 9.883 10.711 15.428 8.078 15.139 15.177 15.484 11.772 16.163 18.675 23.698 19.223 20.898 24.033 97.742 91.314

% transfer % transfer total soluble all protein Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt 3.591 26.830 30.421 65.971 58.183 6.234 33.256 39.490 64.524 54.338 0.000 22.613 22.613 56.295 56.295 0.000 32.921 32.921 67.464 67.464 4.820 35.498 40.318 56.538 49.779 6.606 39.954 46.560 79.782 68.462 0.000 58.970 58.970 74.327 74.327 0.000 49.453 49.453 69.311 69.311 0.456 46.044 46.500 66.371 65.720 4.305 39.115 43.420 69.904 62.973 0.000 27.981 27.981 42.237 42.237 0.000 33.600 33.600 44.421 44.421 6.526 57.053 63.579 58.463 52.462 6.447 127.822 134.269 84.961 80.882 0.000 44.848 44.848 53.402 53.402 0.000 49.425 49.425 51.374 51.374 53.579 181.158 234.737 46.046 35.536 50.246 159.416 209.662 42.720 32.482

IPTG 20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 25 25

0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2 1.03 1.03

interferon

14 hour 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 22 23 24 25 26 27 28

OD 4.64 4.31 13.49 14.01 13.32 13.34 16.05 15.47 3.6 3.48 9.31 8.43 11.69 11.63 13.04 13.09

0 13.142 13.840 14.610 13.34 13.47 15.41 14.36 14.36 12.43 12.95

DCW (g/L) Pr 2.500 2.300 7.905 8.224 7.806 7.816 9.470 9.116 1.867 1.795 5.354 4.819 6.810 6.772 7.634 7.666

0.000 7.695 8.122 8.592 7.82 7.896 9.080 8.44 8.44 7.26 7.58

TC

IB

9.341 7.453 39.542 60.641 59.153 74.047 29.652 29.559 9.502 12.653 13.669 14.544 56.673 97.017 27.848 21.252

3.492 1.564 12.218 18.851 18.451 21.940 17.633 16.721 21.810 34.425 20.554 22.377 32.127 36.062 18.758 33.885

0.000 384.980 0 187.84 191.37 361.209 340.937 180.4 172.78 39.2 44.78

0.000 134.860 20.01 183.27 159.35 353.757 299.324 129.07 120.68 36.58 42.2

% transfer % transfer total soluble all protein Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt 4.820 12.833 17.653 72.789 52.915 3.760 9.017 12.777 82.655 58.331 7.148 51.760 58.908 76.394 67.125 12.715 79.493 92.208 76.285 65.766 3.829 77.604 81.433 76.224 72.640 14.659 95.987 110.646 77.143 66.922 15.797 47.285 63.082 62.710 47.006 11.208 46.280 57.488 63.870 51.418 2.519 31.312 33.831 30.346 28.087 5.852 47.078 52.930 26.877 23.905 16.431 34.223 50.655 39.942 26.986 9.502 36.921 46.423 39.392 31.329 11.089 88.800 99.889 63.821 56.736 6.963 133.079 140.042 72.902 69.277 15.823 46.606 62.429 59.751 44.607 18.666 55.137 73.803 38.543 28.795

0.000 90.790 45.84 220.96 27.55 371.797 349.857 39.57 35.61 28.74 36.04

0.000 0.000 519.840 610.630 20.010 65.850 371.110 592.070 350.718 378.271 714.966 1086.763 640.261 990.118 309.468 349.042 293.461 329.068 75.781 104.524 86.986 123.030

0.000 74.057 0.000 50.616 54.564 50.521 53.250 58.293 58.876 51.731 51.484

0.000 63.046 0.000 31.726 50.589 33.237 34.434 51.683 52.505 37.505 36.401

IPTG 20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30

0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2

25 25 25 25 25 25 25 25 25 25 25

1.03 1.03 0 2.66 2.98 1.03 1.03 1.03 1.03 1.03 1.03

interferon

18 hour 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 22 23 24 25 26 27 28

OD

DCW (g/L) Pr 5.53 3.046 5.86 3.246 11.84 6.900 12.81 7.489 12.69 7.418 12.68 7.411 12.66 7.401 13.25 7.758 4.26 2.272 4.42 2.366 9.79 5.649 10.45 6.048 12.45 7.270 13.3 7.789 13.13 7.686 12.8 7.487

0 11.798 11.200 11.822 15.49 12.73 11.75 15.42 15.42 7.62 9.11

0.000 6.874 6.509 6.889 9.13 7.444 6.844 9.088 9.088 4.32 5.23

TC

IB

7.985 9.985 13.846 28.527 148.287 119.121 27.336 12.851 9.604 16.637 12.432 21.075 194.044 244.892 11.651 12.702

5.427 7.427 11.158 14.879 55.813 44.184 15.353 14.507 28.024 32.053 18.637 21.568 81.415 58.083 20.386 23.596

0.000 362.330 0 140.7 191.81 343.021 340.621 193.416 202.222 55.47 40.97

0.000 112.290 16.26 116.64 130.98 331.578 322.647 131.457 175.931 47.59 42.93

% transfer % transfer total soluble all protein Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt 0.000 13.412 13.412 59.536 59.536 1.078 17.412 18.490 57.346 54.002 6.011 25.004 31.015 55.376 44.644 8.867 43.406 52.273 65.721 54.573 17.000 204.100 221.100 72.654 67.068 11.644 163.305 174.949 72.944 68.089 8.550 42.689 51.239 64.035 53.350 7.016 27.358 34.373 46.973 37.386 2.321 37.628 39.949 25.524 24.041 0.655 48.690 49.345 34.169 33.716 8.259 31.070 39.328 40.014 31.611 12.067 42.643 54.710 49.422 38.521 21.682 275.459 297.141 70.444 65.304 16.683 302.975 319.658 80.829 76.611 7.651 32.037 39.688 36.366 29.356 9.634 36.298 45.932 34.994 27.654

0.000 67.270 30.21 131.22 54.41 371.082 389.903 76.708 41.082 40.33 42.55

0.000 0.000 474.620 541.890 16.260 46.470 257.340 388.560 322.794 377.206 674.599 1045.681 663.268 1053.171 324.874 401.582 378.153 419.235 103.062 143.390 83.902 126.452

0.000 76.341 0.000 54.675 59.422 50.848 51.355 59.536 53.476 53.825 48.836

0.000 66.864 0.000 36.211 50.851 32.804 32.342 48.164 48.236 38.687 32.403

IPTG 20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30

0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2

25 25 25 25 25 25 25 25 25 25 25

1.03 1.03 0 2.66 2.98 1.03 1.03 1.03 1.03 1.03 1.03

OD induce 1 1 1 1 7 7 7 7 1 1 1 1 7 7 7 7 4 4

OD induce 1 1 1 1 7 7 7 7 1 1 1 1 7 7 7 7 4 4 0 8 4 4 4 4 4 4 4 4 4

14 14 14

13.34 15.49

7.82 9.13

14 18

OD induce 1 1 1 1 7 7 7 7 1 1 1 1 7 7 7 7 4 4 0 8 4 4 4 4 4 4 4 4 4

18 18 18

14.36 15.42

8.44 9.09

14 18

14.36 15.42

8.44 9.09

14 18

interferon % transfer % transfer total soluble all protein 10 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt 1 2.47 1.179 17.700 9.130 3.591 26.830 30.421 65.971 58.183 2 3 1.500 21.458 11.798 6.234 33.256 39.490 64.524 54.338 3 11.72 0.582 1.087 0.844 0.000 1.930 1.930 56.295 56.295 4 11.92 0.583 22.210 10.711 0.000 32.921 32.921 67.464 67.464 5 10.92 6.337 20.070 15.428 4.820 35.498 40.318 56.538 49.779 6 14.49 8.517 31.876 8.078 6.606 39.954 46.560 79.782 68.462 7 13.96 8.196 43.830 15.139 0.000 58.970 58.970 74.327 74.327 8 13.98 8.204 34.276 15.177 0.000 49.453 49.453 69.311 69.311 9 2.47 1.179 30.560 15.484 0.456 46.044 46.500 66.371 65.720 10 3.34 1.705 27.343 11.772 4.305 39.115 43.420 69.904 62.973 11 8.04 4.579 11.818 16.163 0.000 27.981 27.981 42.237 42.237 12 7.88 4.480 14.925 18.675 0.000 33.600 33.600 44.421 44.421 13 9.56 5.510 33.355 23.698 6.526 57.053 63.579 58.463 52.462 14 13.77 8.077 108.599 19.223 6.447 127.822 134.269 84.961 80.882 15 13.22 7.745 23.950 20.898 0.000 44.848 44.848 53.402 53.402 16 12.49 7.297 25.392 24.033 0.000 49.425 49.425 51.374 51.374 17 12.34 7.205 83.416 97.742 53.579 181.158 234.737 46.046 35.536 18 13.26 7.766 68.102 91.314 50.246 159.416 209.662 42.720 32.482 19 20 21 22 23 24 25 26 27 28

10 hour

OD

DCW (g/L) Pr/OD

TC/OD

IB/OD

Pr + TC

(Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100

20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 25 25

IPTG OD induce 0.05 1 0.05 1 0.05 7 0.05 7 0.05 1 0.05 1 0.05 7 0.05 7 2 1 2 1 2 7 2 7 2 1 2 1 2 7 2 7 1.03 4 1.03 4

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

2.47 3 11.72 11.92 10.92 14.49 13.96 13.98 2.47 3.34 8.04 7.88 9.56 13.77 13.22 12.49 12.34 13.26

1.179 1.500 0.582 0.583 6.337 8.517 8.196 8.204 1.179 1.705 4.579 4.480 5.510 8.077 7.745 7.297 7.205 7.766

7.154 7.152 0.093 1.864 1.838 2.200 3.139 2.453 12.352 8.199 1.470 1.894 3.488 7.888 1.811 2.033 6.760 5.137

3.690 3.932 0.072 0.899 1.413 0.558 1.084 1.086 6.259 3.530 2.010 2.370 2.478 1.396 1.580 1.924 7.921 6.887

1.451 2.078 0.000 0.000 0.441 0.456 0.000 0.000 0.184 1.291 0.000 0.000 0.682 0.468 0.000 0.000 4.342 3.790

10.845 11.085 0.165 2.763 3.251 2.758 4.224 3.539 18.611 11.729 3.480 4.265 5.965 9.285 3.391 3.957 14.681 12.024

12.296 13.162 0.165 2.763 3.693 3.214 4.224 3.539 18.795 13.019 3.480 4.265 6.648 9.753 3.391 3.957 19.022 15.814

65.971 64.524 56.295 67.464 56.538 79.782 74.327 69.311 66.371 69.904 42.237 44.421 58.463 84.961 53.402 51.374 46.046 42.720

58.183 54.338 56.295 67.464 49.779 68.462 74.327 69.311 65.720 62.973 42.237 44.421 52.462 80.882 53.402 51.374 35.536 32.482

3.492

% transfer % transfer total soluble all protein Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt IPTG OD induce 4.820 12.833 17.653 72.789 52.915 20 0.05 1

interferon

14 hour

OD 1

DCW (g/L) Pr 4.64 2.500

TC 9.341

IB

2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

14 hour

4.31 13.49 14.01 13.32 13.34 16.05 15.47 3.6 3.48 9.31 8.43 11.69 11.63 13.04 13.09 8.69 11.79 0 13.142 13.840 13.340 13.47 15.41 14.36 14.25 12.43 12.95

OD 1 2 3 4 5

2.300 7.905 8.224 7.806 7.816 9.470 9.116 1.867 1.795 5.354 4.819 6.810 6.772 7.634 7.665 4.976 6.870 -0.333 7.695 8.122 7.816 7.896 9.080 8.437 8.372 7.262 7.581

7.453 59.542 60.641 69.153 74.047 29.652 29.559 9.502 12.653 13.669 14.544 86.673 97.017 27.848 21.252 25.96 10.14 0.000 184.980 0 191.37 361.209 394.937 380.4 307.421 316.202 334.784

1.564 12.218 18.851 18.451 21.940 17.633 16.721 21.810 24.425 20.554 22.377 32.127 36.062 28.758 33.885 56.7 5.75 0.000 134.860 20.01 159.35 353.757 365.324 339.07 314.802 322.579 312.202

3.760 17.148 12.715 3.829 14.659 15.797 11.208 2.519 5.852 16.431 9.502 11.089 6.963 15.823 18.666 52.94 5.06 0.000 90.790 45.84 189.36 371.797 389.857 345.574 289.607 316.743 348.043

9.017 12.777 71.760 88.908 79.493 92.208 87.604 91.433 95.987 110.646 47.285 63.082 46.280 57.488 31.312 33.831 37.078 42.930 34.223 50.655 36.921 46.423 118.800 129.889 133.079 140.042 56.606 72.429 55.137 73.803 82.660 135.604 15.891 20.954 0.000 0.000 319.840 410.630 20.010 65.850 350.718 540.078 714.966 1086.763 760.261 1150.118 719.468 1065.042 622.223 911.830 638.781 955.524 646.986 995.030

82.655 82.974 76.285 78.938 77.143 62.710 63.870 30.346 34.125 39.942 39.392 72.957 72.902 49.196 38.543 31.404 63.800 0.000 57.835 0.000 54.564 50.521 51.948 52.872 49.407 49.501 51.745

58.331 66.970 65.766 75.632 66.922 47.006 51.418 28.087 29.474 26.986 31.329 66.729 69.277 38.448 28.795 19.143 48.384 0.000 45.048 0.000 35.433 33.237 34.339 35.717 33.715 33.092 33.646

total % soluble DCW (g/L) Pr/OD TC/OD IB/OD Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 4.637 2.500 2.014 0.753 1.039 2.768 3.807 72.789 52.915 4.310 2.300 1.729 0.363 0.872 2.092 2.965 82.655 58.331 13.485 7.905 4.415 0.906 1.272 5.321 6.593 82.974 66.970 14.007 8.224 4.329 1.346 0.908 5.675 6.583 76.285 65.766 13.323 7.806 5.190 1.385 0.287 6.575 6.863 78.938 75.632

20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 15 35 25 25 25 25 25 25 25 25 25 25

0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2 1.03 1.03 1.03 1.03 0 2.98 1.03 1.03 1.03 1.03 1.03 1.03

1 7 7 1 1 7 7 1 1 7 7 1 1 7 7 4 4 0 8 4 4 4 4 4 4 4 4

6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

13.339 16.047 15.468 3.601 3.483 9.309 8.433 11.692 11.631 13.041 13.093 8.691 11.792 0.000 13.142 13.840 14.610 13.470 15.409 9.466 11.054 12.432 12.954

7.816 9.470 9.116 1.867 1.795 5.354 4.819 6.810 6.772 7.634 7.666 4.976 6.870 0.000 7.695 8.122 8.592 7.896 9.080 5.450 6.420 7.262 7.581

5.551 1.848 1.911 2.639 3.633 1.468 1.725 7.413 8.341 2.135 1.623 2.987 0.860 0.000 14.075 0.000 #REF! 26.816 25.630 #REF! 27.811 25.435 25.844

1.645 1.099 1.081 6.057 7.013 2.208 2.654 2.748 3.101 2.205 2.588 6.524 0.488 0.000 10.262 1.446 #REF! 26.263 23.708 #REF! 28.479 25.947 24.101

1.099 0.984 0.725 0.700 1.680 1.765 1.127 0.948 0.599 1.213 1.426 6.092 0.429 0.000 6.908 3.312 14.195 27.602 25.301 36.507 26.199 25.478 26.868

7.196 2.947 2.992 8.695 10.645 3.676 4.378 10.161 11.442 4.341 4.211 9.511 1.348 0.000 24.337 1.446 #REF! 53.078 49.339 #REF! 56.289 51.382 49.945

8.295 3.931 3.717 9.395 12.326 5.441 5.505 11.109 12.040 5.554 5.637 15.603 1.777 0.000 31.246 4.758 #REF! 80.680 74.639 #REF! 82.489 76.860 76.813

77.143 62.710 63.870 30.346 34.125 39.942 39.392 72.957 72.902 49.196 38.543 31.404 63.800 1.000 57.835 0.000 #REF! 50.521 51.948 #REF! 49.407 49.501 51.745

66.922 47.006 51.418 28.087 29.474 26.986 31.329 66.729 69.277 38.448 28.795 19.143 48.384 2.000 45.048 0.000 #REF! 33.237 34.339 #REF! 33.715 33.092 33.646

interferon % transfer % transfer total soluble all protein 18 hour OD DCW (g/L) Pr TC IB Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt 1 5.53 3.045 7.985 5.427 0.000 13.412 13.412 59.536 59.536 2 5.86 3.246 9.985 7.427 1.078 17.412 18.490 57.346 54.002 3 11.84 6.899 13.846 11.158 6.011 25.004 31.015 55.376 44.644 4 12.81 7.489 28.527 14.879 8.867 43.406 52.273 65.721 54.573 5 12.69 7.418 148.287 55.813 17.000 204.100 221.100 72.654 67.068 6 12.68 7.411 119.121 44.184 11.644 163.305 174.949 72.944 68.089 7 12.66 7.402 27.336 15.353 8.550 42.689 51.239 64.035 53.350

IPTG OD induce 20 0.05 1 20 0.05 1 30 0.05 7 30 0.05 7 20 0.05 1 20 0.05 1 30 0.05 7

8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

18 hour 1 2 3 4 5 6 7 8 9 10 11 12

13.25 4.26 4.42 9.79 10.45 12.45 13.3 13.13 12.8 9.31 11.03 0 11.798 11.200 15.490 12.73 11.75 15.42 15.42 7.62 9.11

OD

7.758 2.271 2.366 5.650 6.048 7.270 7.789 7.686 7.487 5.351 6.406 0.000 6.874 6.509 9.130 7.444 6.844 9.088 9.088 4.321 5.234

12.851 9.604 16.637 12.432 21.075 194.044 244.892 11.651 12.702 136.66 3.69 0.000 362.330 0 191.81 343.021 340.621 193.42 210.32 205.47 245.97

14.507 28.024 32.053 18.637 21.568 81.415 58.083 20.386 23.596 95.94 13.99 0.000 112.290 16.26 130.98 331.578 322.647 131.46 207.7 203.59 235.93

7.016 2.321 0.655 8.259 12.067 21.682 16.683 7.651 9.634 88.41 13.75 0.000 67.270 30.21 131.22 371.082 309.903 260.71 210.08 230.33 252.55

27.358 34.373 37.628 39.949 48.690 49.345 31.070 39.328 42.643 54.710 275.459 297.141 302.975 319.658 32.037 39.688 36.298 45.932 232.605 321.016 17.673 31.420 0.000 0.000 474.620 541.890 16.260 46.470 322.794 454.014 674.599 1045.681 663.268 973.171 324.874 585.582 418.020 628.102 409.062 639.390 481.902 734.452

46.973 25.524 34.169 40.014 49.422 70.444 80.829 36.366 34.994 58.753 20.863 0.000 76.341 0.000 59.422 50.848 51.355 59.536 50.313 50.230 51.042

37.386 24.041 33.716 31.611 38.521 65.304 76.611 29.356 27.654 42.572 11.735 0.000 66.864 0.000 42.248 32.804 35.001 33.030 33.485 32.136 33.491

total % transfer soluble DCW (g/L) Pr/OD TC/OD IB/OD Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 5.530 3.045 1.444 0.981 0.000 2.425 2.425 59.536 59.536 5.858 3.246 1.705 1.268 0.184 2.972 3.156 57.346 54.002 11.839 6.899 1.170 0.942 0.508 2.112 2.620 55.376 44.644 12.805 7.489 2.228 1.162 0.692 3.390 4.082 65.721 54.573 12.688 7.418 11.687 4.399 1.340 16.086 17.426 72.654 67.068 12.676 7.411 9.397 3.486 0.919 12.883 13.802 72.944 68.089 12.661 7.402 2.159 1.213 0.675 3.372 4.047 64.035 53.350 13.245 7.758 0.970 1.095 0.530 2.066 2.595 46.973 37.386 4.263 2.271 2.253 6.574 0.544 8.827 9.371 25.524 24.041 4.418 2.366 3.766 7.255 0.148 11.021 11.169 34.169 33.716 9.793 5.650 1.269 1.903 0.843 3.173 4.016 40.014 31.611 10.445 6.048 2.018 2.065 1.155 4.083 5.238 49.422 38.521

30 20 20 30 30 20 20 30 30 15 35 25 25 25 25 25 25 25 25 25 25

0.05 2 2 2 2 2 2 2 2 1.03 1.03 1.03 1.03 0 2.98 1.03 1.03 1.03 1.03 1.03 1.03

7 1 1 7 7 1 1 7 7 4 4 0 8 4 4 4 4 4 4 4 4

13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

12.446 13.296 13.127 12.801 9.305 11.032 0.000 11.798 11.200 11.822 12.731 11.748 7.830 7.344 7.618 9.112

7.270 7.789 7.686 7.487 5.351 6.406 -0.333 6.874 6.509 6.889 7.444 6.844 4.450 4.154 4.321 5.234

15.591 18.418 0.888 0.992 14.687 0.334 0.000 30.711 0.000 12.383 26.944 28.994 12.542 13.638 26.972 26.995

6.541 4.368 1.553 1.843 10.311 1.268 0.000 9.518 1.452 8.456 26.045 27.464 8.524 13.468 26.725 25.892

1.742 1.255 0.583 0.753 9.502 1.246 0.000 5.702 2.697 8.471 29.148 26.379 33.296 28.606 30.235 27.716

22.132 22.787 2.441 2.836 24.998 1.602 0.000 40.229 1.452 20.839 52.989 56.458 21.066 27.105 53.697 52.887

23.874 24.042 3.023 3.588 34.499 2.848 0.000 45.931 4.149 29.310 82.137 82.837 54.362 55.711 83.932 80.603

70.444 80.829 36.366 34.994 58.753 20.863 0.000 76.341 0.000 59.422 50.848 51.355 59.536 50.313 50.230 51.042

65.304 76.611 29.356 27.654 42.572 11.735 0.000 66.864 0.000 42.248 32.804 35.001 23.071 24.479 32.136 33.491

Specific ifn production (ng/mL/OD)

10 hour

OD 1 2 3

2.47 3.00 11.72

DCW (g/L) Pr 1.18 1.50 0.58

TC 7.15 7.15 0.09

IB 3.69 3.93 0.07

% transfer % transfer total soluble all protein Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt IPTG OD induce 1.45 10.84 12.30 65.97 58.18 20 0.05 1 2.08 11.08 13.16 64.52 54.34 20 0.05 1 0.00 0.16 0.16 56.29 56.29 30 0.05 7

4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

11.92 10.92 14.49 13.96 13.98 2.47 3.34 8.04 7.88 9.56 13.77 13.22 12.49 12.34 13.26

0.58 6.34 8.52 8.20 8.20 1.18 1.71 4.58 4.48 5.51 8.08 7.75 7.30 7.21 7.77

1.86 1.84 2.20 3.14 2.45 12.35 8.20 1.47 1.89 3.49 7.89 1.81 2.03 6.76 5.14

0.90 1.41 0.56 1.08 1.09 6.26 3.53 2.01 2.37 2.48 1.40 1.58 1.92 7.92 6.89

0.00 0.44 0.46 0.00 0.00 0.18 1.29 0.00 0.00 0.68 0.47 0.00 0.00 4.34 3.79

2.76 3.25 2.76 4.22 3.54 18.61 11.73 3.48 4.26 5.97 9.28 3.39 3.96 14.68 12.02

2.76 3.69 3.21 4.22 3.54 18.80 13.02 3.48 4.26 6.65 9.75 3.39 3.96 19.02 15.81

67.46 56.54 79.78 74.33 69.31 66.37 69.90 42.24 44.42 58.46 84.96 53.40 51.37 46.05 42.72

67.46 49.78 68.46 74.33 69.31 65.72 62.97 42.24 44.42 52.46 80.88 53.40 51.37 35.54 32.48

0.75 0.36 0.91 1.35 1.38 1.64 1.10 1.08 6.06 9.88 2.21 2.65 2.75 3.10 1.44

% transfer % transfer total soluble all protein Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt 1.04 2.77 3.81 72.79 52.91 0.87 2.09 2.96 82.65 58.33 0.53 3.84 4.37 76.39 67.12 0.91 5.68 6.58 76.29 65.77 0.29 5.82 6.11 76.22 72.64 1.10 7.20 8.29 77.14 66.92 0.98 2.95 3.93 62.71 47.01 0.72 2.99 3.72 63.87 51.42 0.70 8.70 9.39 30.35 28.09 1.68 13.52 15.20 26.88 23.91 1.77 3.68 5.44 39.94 26.99 1.13 4.38 5.50 39.39 31.33 0.95 7.59 8.54 63.82 56.74 0.60 11.44 12.04 72.90 69.28 1.21 3.57 4.79 59.75 44.61

30 20 20 30 30 20 20 30 30 20 20 30 30 25 25

0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2 1.03 1.03

7 1 1 7 7 1 1 7 7 1 1 7 7 4 4

Specific ifn production (ng/mL/OD)

14 hour 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

OD 4.64 4.31 13.49 14.01 13.32 13.34 16.05 15.47 3.60 3.48 9.31 8.43 11.69 11.63 13.04

DCW (g/L) Pr 2.50 2.30 7.90 8.22 7.81 7.82 9.47 9.12 1.87 1.80 5.35 4.82 6.81 6.77 7.63

TC 2.01 1.73 2.93 4.33 4.44 5.55 1.85 1.91 2.64 3.63 1.47 1.72 4.85 8.34 2.14

IB

20 20 30 30 20 20 30 30 20 20 30 30 20 20 30

IPTG OD induce 0.05 1 0.05 1 0.05 7 0.05 7 0.05 1 0.05 1 0.05 7 0.05 7 2 1 2 1 2 7 2 7 2 1 2 1 2 7

16 17 18

13.09 13.47 15.41

7.67 7.90 9.08

1.62 26.82 22.13

2.59 26.26 19.43

1.43 27.60 22.70

4.21 53.08 41.55

5.64 80.68 64.26

38.54 50.52 53.25

28.79 33.24 34.43

0.98 1.27 0.94 1.16 4.40 3.49 1.21 1.10 6.57 7.26 1.90 2.06 6.54 4.37 1.55 1.84 26.04 27.46

% transfer % transfer total soluble all protein Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 tempt 0.00 2.43 2.43 59.54 59.54 0.18 2.97 3.16 57.35 54.00 0.51 2.11 2.62 55.38 44.64 0.69 3.39 4.08 65.72 54.57 1.34 16.09 17.43 72.65 67.07 0.92 12.88 13.80 72.94 68.09 0.68 3.37 4.05 64.03 53.35 0.53 2.07 2.60 46.97 37.39 0.54 8.83 9.37 25.52 24.04 0.15 11.02 11.17 34.17 33.72 0.84 3.17 4.02 40.01 31.61 1.16 4.08 5.24 49.42 38.52 1.74 22.13 23.87 70.44 65.30 1.25 22.79 24.04 80.83 76.61 0.58 2.44 3.02 36.37 29.36 0.75 2.84 3.59 34.99 27.65 29.15 52.99 82.14 50.85 32.80 41.70 56.46 98.16 51.35 29.54

30 25 25

2 1.03 1.03

7 4 4

Specific ifn production (ng/mL/OD)

18 hour 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

OD 5.53 5.86 11.84 12.81 12.69 12.68 12.66 13.25 4.26 4.42 9.79 10.45 12.45 13.30 13.13 12.80 12.73 11.75

DCW (g/L) Pr 3.05 3.25 6.90 7.49 7.42 7.41 7.40 7.76 2.27 2.37 5.65 6.05 7.27 7.79 7.69 7.49 7.44 6.84

TC 1.44 1.70 1.17 2.23 11.69 9.40 2.16 0.97 2.25 3.77 1.27 2.02 15.59 18.42 0.89 0.99 26.94 28.99

IB

20 20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 25 25

IPTG OD induce 0.05 1 0.05 1 0.05 7 0.05 7 0.05 1 0.05 1 0.05 7 0.05 7 2 1 2 1 2 7 2 7 2 1 2 1 2 7 2 7 1.03 4 1.03 4

OD induce

Protein OD induce

14 hour

OD 1

DCW (g/L) Pr 4.64 2.500

TC 0.02

IB 0.11

% transfer % transfer total soluble all protein Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 0.1 0.127 0.232 13.928 7.631

2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

4.31 13.49 14.01 13.32 13.34 16.05 15.47 3.6 3.48 9.31 8.43 11.69 11.63 13.04 13.09 8.69 11.79 0 13.142 13.840 13.34 13.47 15.41 9.47 11.05 12.43 12.95

2.300 7.905 8.224 7.806 7.816 9.470 9.116 1.867 1.795 5.354 4.819 6.810 6.772 7.634 7.666 4.98 6.87 0 7.695 8.122 7.82 7.896 9.080 5.450 6.420 7.262 7.581

0.02 0.22 0.26 0.14 0.22 0.22 0.27 0.02 0.01 0.12 0.08 0.1 0.16 0.16 0.21 0.14 0.16 0 0.34 0.34 0.43 0.34 0.35 0.46 0.46 0.46 0.46

0.11 0.29 0.28 0.18 0.22 0.32 0.33 0.11 0.14 0.23 0.22 0.21 0.19 0.29 0.32 0.39 0.44 0 0.41 0.41 0.3 0.41 0.52 0.31 0.31 0.31 0.31

0.1 0 0.05 0.04 0.04 0.05 0.05 0.11 0.05 0 0 0.09 0.13 0 0 0.2 0.15 0 0.15 0.15 0.15 0.15 0.18 0.19 0.19 0.19 0.19

0.127 0.513 0.542 0.324 0.445 0.541 0.595 0.127 0.154 0.347 0.300 0.319 0.353 0.447 0.524 0.533 0.596 0.000 0.742 0.742 0.726 0.742 0.865 0.774 0.774 0.774 0.774

0.232 0.513 0.594 0.366 0.483 0.589 0.645 0.236 0.205 0.347 0.300 0.409 0.480 0.447 0.524 0.730 0.746 0.000 0.891 0.891 0.875 0.891 1.050 0.968 0.968 0.968 0.968

13.928 43.657 48.463 44.129 50.292 41.568 44.868 12.983 6.050 34.334 26.459 32.779 46.316 34.715 39.842 26.816 26.349 0.000 45.195 45.195 58.854 45.195 39.973 59.478 59.478 59.478 59.478

7.631 43.657 44.236 39.104 46.315 38.138 41.444 6.999 4.523 34.334 26.459 25.612 34.061 34.715 39.842 19.593 21.038 0.000 37.669 37.669 48.846 37.669 32.930 47.552 47.552 47.552 47.552

microg/mL 14 hour OD DCW (g/L) Pr/OD TC/OD IB/OD Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 1 4.64 2.500 3.820 23.607 22.632 27.427 50.059 13.928 7.631 2 4.31 2.300 4.110 25.399 24.350 29.510 53.860 13.928 7.631 3 13.49 7.905 16.601 21.425 0.000 38.026 38.026 43.657 43.657 4 14.01 8.224 18.759 19.949 3.699 38.707 42.406 48.463 44.236 5 13.32 7.806 10.745 13.604 3.129 24.349 27.478 44.129 39.104

6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

13.34 16.05 15.47 3.6 3.48 9.31 8.43 11.69 11.63 13.04 13.09 8.69 11.79 0 13.142 13.840 13.34 13.47 15.41 9.47 11.05 12.43 12.95

7.816 9.470 9.116 1.867 1.795 5.354 4.819 6.810 6.772 7.634 7.666 4.98 6.87 0.000 7.695 8.122 7.82 7.896 9.080 5.450 6.420 7.262 7.581

16.785 14.008 17.271 4.589 2.668 12.795 9.403 8.957 14.053 11.894 15.949 16.448 13.311 0.000 25.533 24.246 32.024 24.912 22.440 48.612 41.629 37.014 35.523

16.590 19.692 21.222 30.759 41.439 24.471 26.135 18.369 16.289 22.367 24.082 44.888 37.208 0.000 30.963 29.401 22.389 30.209 33.698 33.119 28.361 25.218 24.201

2.866 3.030 3.181 30.222 14.882 0.000 0.000 7.646 10.916 0.000 0.000 22.610 12.752 0.000 11.287 10.718 11.148 11.012 12.006 20.498 17.553 15.608 14.979

protein OD induce

18 hour

OD 1 2 3 4 5 6 7

DCW (g/L) Pr 5.530 3.046 5.858 3.246 11.839 6.900 12.805 7.489 12.688 7.418 12.676 7.411 12.661 7.401

TC 0.045 0.045 0.111 0.162 0.226 0.294 0.259

IB 0.142 0.142 0.324 0.304 0.168 0.213 0.200

33.376 33.700 38.493 35.348 44.108 37.266 35.537 27.326 30.342 34.261 40.031 61.336 50.519 0.000 56.496 53.647 54.414 55.120 56.137 81.731 69.990 62.232 59.724

36.242 36.730 41.674 65.570 58.990 37.266 35.537 34.972 41.258 34.261 40.031 83.946 63.271 0.000 67.783 64.365 65.562 66.133 68.143 102.229 87.543 77.840 74.703

50.292 41.568 44.868 12.983 6.050 34.334 26.459 32.779 46.316 34.715 39.842 26.816 26.349 0.000 45.195 45.195 58.854 45.195 39.973 59.478 59.478 59.478 59.478

46.315 38.138 41.444 6.999 4.523 34.334 26.459 25.612 34.061 34.715 39.842 19.593 21.038 0.000 37.669 37.669 48.846 37.669 32.930 47.552 47.552 47.552 47.552

% transfer % transfer total soluble all protein Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 0.081 0.187 0.268 24.216 16.879 0.081 0.187 0.268 24.216 16.879 0.047 0.435 0.482 25.588 23.094 0.000 0.466 0.466 34.825 34.825 0.148 0.394 0.542 57.371 41.722 0.140 0.507 0.647 58.049 45.486 0.000 0.459 0.459 56.416 56.416

8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 microg/mL 18 hour OD 1 2 3 4 5 6 7 8 9 10 11 12

13.245 4.263 4.418 9.793 10.445 12.446 13.296 13.127 12.801 9.305 11.032 0.000 11.798 11.200 15.490 12.731 11.748 7.830 7.344 7.618 9.112

7.758 2.272 2.366 5.649 6.048 7.270 7.789 7.686 7.487 5.351 6.406 0.000 6.874 6.509 9.130 7.444 6.844 4.450 4.154 4.321 5.234

0.150 0.042 0.045 0.153 0.231 0.329 0.286 0.145 0.121 0.222 0.243 0.000 0.341 0.341 0.550 0.341 0.343 0.441 0.441 0.441 0.441

0.162 0.106 0.134 0.211 0.204 0.225 0.116 0.194 0.199 0.326 0.416 0.000 0.511 0.511 0.328 0.511 0.494 0.299 0.299 0.299 0.299

0.000 0.055 0.042 0.000 0.000 0.092 0.082 0.000 0.000 0.124 0.179 0.000 0.187 0.187 0.242 0.187 0.169 0.286 0.286 0.286 0.286

0.312 0.149 0.179 0.364 0.435 0.554 0.401 0.339 0.320 0.548 0.659 0.000 0.852 0.852 0.877 0.852 0.836 0.740 0.740 0.740 0.740

0.312 0.204 0.221 0.364 0.435 0.646 0.483 0.339 0.320 0.672 0.838 0.000 1.039 1.039 1.119 1.039 1.005 1.026 1.026 1.026 1.026

47.943 28.523 25.199 42.124 53.036 59.441 71.194 42.694 37.761 40.526 36.813 0.000 40.052 40.052 62.676 40.052 40.992 59.633 59.633 59.633 59.633

47.943 20.779 20.392 42.124 53.036 50.981 59.176 42.694 37.761 33.067 28.965 0.000 32.842 32.842 49.135 32.842 34.102 42.995 42.995 42.995 42.995

DCW (g/L) Pr/OD TC/OD IB/OD Pr + TC (Pr+TC+IB)Pr/(Pr+TC)*100 (Pr/total)*100 5.53 3.046 8.181 25.602 0.015 33.783 33.798 24.216 24.205 5.86 3.246 7.723 24.169 0.014 31.891 31.905 24.216 24.205 11.84 6.900 9.407 27.356 0.004 36.763 36.766 25.588 25.585 12.81 7.489 12.674 23.719 0.000 36.393 36.393 34.825 34.825 12.69 7.418 17.817 13.239 0.012 31.055 31.067 57.371 57.349 12.68 7.411 23.232 16.790 0.011 40.022 40.033 58.049 58.033 12.66 7.401 20.461 15.807 0.000 36.267 36.267 56.416 56.416 13.25 7.758 11.292 12.261 0.000 23.553 23.553 47.943 47.943 4.26 2.272 9.954 24.944 0.013 34.898 34.911 28.523 28.512 4.42 2.366 10.215 30.324 0.010 40.539 40.549 25.199 25.193 9.79 5.649 15.650 21.502 0.000 37.153 37.153 42.124 42.124 10.45 6.048 22.076 19.548 0.000 41.623 41.623 53.036 53.036

13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

OD induce

12.45 13.3 13.13 12.8 9.31 11.03 0 11.798 11.200 15.49 12.73 11.75 7.83 7.34 7.62 9.11

7.270 7.789 7.686 7.487 5.35 6.41 0.000 6.874 6.509 9.13 7.444 6.844 4.45 4.15 4.32 5.23

26.472 21.492 11.023 9.441 23.863 21.995 0.000 28.930 30.475 35.505 26.810 29.187 56.349 60.078 57.917 48.421

18.062 8.696 14.796 15.561 35.020 37.753 0.000 43.301 45.613 21.143 40.128 42.014 38.145 40.669 39.206 32.778

0.007 0.006 0.000 0.000 0.013 0.016 0.000 0.016 0.017 0.016 0.015 0.014 0.037 0.039 0.038 0.031

44.534 30.187 25.819 25.003 58.884 59.748 0.000 72.231 76.088 56.648 66.938 71.202 94.494 100.747 97.123 81.199

44.542 30.193 25.819 25.003 58.897 59.764 0.000 72.247 76.105 56.664 66.953 71.216 94.530 100.786 97.161 81.231

59.441 71.194 42.694 37.761 40.526 36.813 0.000 40.052 40.052 62.676 40.052 40.992 59.633 59.633 59.633 59.633

59.432 71.179 42.694 37.761 40.517 36.803 0.000 40.043 40.043 62.659 40.043 40.984 59.609 59.609 59.609 59.609

OD induce

OD induce

tempt

IPTG 20

OD induce 0.05 1

Pr/total)*100

20 30 30 20 20 30 30 20 20 30 30 20 20 30 30 15 35 25 25 25 25 25 25 25 25 25 25

0.05 0.05 0.05 0.05 0.05 0.05 0.05 2 2 2 2 2 2 2 2 1.03 1.03 1.03 1.03 0 2.98 1.03 1.03 1.03 1.03 1.03 1.03

1 7 7 1 1 7 7 1 1 7 7 1 1 7 7 4 4 0 8 4 4 4 4 4 4 4 4

tempt

IPTG 20 20 30 30 20 20 30

OD induce 0.05 1 0.05 1 0.05 7 0.05 7 0.05 1 0.05 1 0.05 7

Pr/total)*100

30 20 20 30 30 20 20 30 30 15 35 25 25 25 25 25 25 25 25 25 25

0.05 2 2 2 2 2 2 2 2 1.03 1.03 1.03 1.03 0 2.98 1.03 1.03 1.03 1.03 1.03 1.03

7 1 1 7 7 1 1 7 7 4 4 0 8 4 4 4 4 4 4 4 4