Capillary Electrophoresis By Sunil Budke
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CAPILLARY ELECTROPHORESIS BASIC CONCEPTS & HARDWARE
by Sunil
Budke
CE by SUNIL BUDKE 10/25/09 23:36
Capillary tube
Destination Vial Source vial
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Electropherogram from CE
CAPILLARY
Made of fused silica Polyamide coating for protection Pyrex or Teflon capillaries are available Effective length 10 - 100 cm Internal diameter (ID) 20 -100 micro meter C8 and C18 coated capillaries are available
SAMPLE INTRODUCTION Electrokinetic Injection 5kv for 5 – 10 sec
Gravimetric (Syphonic) Sample vial held at higher level
Differential pressure Pressure applied on sample vial or vacuum applied on Destination vial
DETECTORS
Conductivity Detectors
UV Detectors
Fluorescence Detector
Electrochemical Detector
Mass Spectrometer
CE by SUNIL BUDKE 10/25/09 23:36
DISCUSSION
References
Capillary Electrophoresis -by Dale R. Baker
Capillary Electrophoresis for Food Analysis – Method Development -by Richard A. Frazier; Jennifer M. Ames and Harry E. Nursten
Principles and Practice of Bioanalysis -by Richard F. Venn
McGraw Hill - Year book of Science and Technology – 2002 -Capillary electrophoresis
Thank You
CE by SUNIL BUDKE 10/25/09 23:36
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by Sunil
Budke
CE by SUNIL BUDKE 10/25/09 23:36
Capillary tube
Destination Vial Source vial
+
_
CE by SUNIL BUDKE 10/25/09 23:36
+
_
CE by SUNIL BUDKE 10/25/09 23:36
+
_
CE by SUNIL BUDKE 10/25/09 23:36
CE by SUNIL BUDKE 10/25/09 23:36
CE by SUNIL BUDKE 10/25/09 23:36
CE by SUNIL BUDKE 10/25/09 23:36
CE by SUNIL BUDKE 10/25/09 23:36
CE by SUNIL BUDKE 10/25/09 23:36
CE by SUNIL BUDKE 10/25/09 23:36
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CE by SUNIL BUDKE 10/25/09 23:36
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CE by SUNIL BUDKE 10/25/09 23:36
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CE by SUNIL BUDKE 10/25/09 23:36
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CE by SUNIL BUDKE 10/25/09 23:36
+ + + _N _ N
CE by SUNIL BUDKE 10/25/09 23:36
+ + + _N _ N
CE by SUNIL BUDKE 10/25/09 23:36
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N
_
N
++ +
CE by SUNIL BUDKE 10/25/09 23:36
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CE by SUNIL BUDKE 10/25/09 23:36
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CE by SUNIL BUDKE 10/25/09 23:36
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N
++
CE by SUNIL BUDKE 10/25/09 23:36
_
N
_
N
++
CE by SUNIL BUDKE 10/25/09 23:36
+
_
Electropherogram from CE
CAPILLARY
Made of fused silica Polyamide coating for protection Pyrex or Teflon capillaries are available Effective length 10 - 100 cm Internal diameter (ID) 20 -100 micro meter C8 and C18 coated capillaries are available
SAMPLE INTRODUCTION Electrokinetic Injection 5kv for 5 – 10 sec
Gravimetric (Syphonic) Sample vial held at higher level
Differential pressure Pressure applied on sample vial or vacuum applied on Destination vial
DETECTORS
Conductivity Detectors
UV Detectors
Fluorescence Detector
Electrochemical Detector
Mass Spectrometer
CE by SUNIL BUDKE 10/25/09 23:36
DISCUSSION
References
Capillary Electrophoresis -by Dale R. Baker
Capillary Electrophoresis for Food Analysis – Method Development -by Richard A. Frazier; Jennifer M. Ames and Harry E. Nursten
Principles and Practice of Bioanalysis -by Richard F. Venn
McGraw Hill - Year book of Science and Technology – 2002 -Capillary electrophoresis
Thank You
CE by SUNIL BUDKE 10/25/09 23:36
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