Biotechnology Fresh Graduate Resume Sandeep
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SANDEEP MALLAREDDY 505, Sunnyside Homes, Opp. Apollo Hospitals, Waltair Main Road, Visakhapatnam-530002. Tel: (91) 9908166652. Email: [email protected]
------------------------------------------------------------------------------------------------------------------------------OBJECTIVE To apply my knowledge and experience in solving biological problems and developing sustainable and novel technologies. EDUCATION Master of Science In Biology (GPA 4.0/4.0) University of Kentucky, Lexington, Kentucky USA.
[Aug 2006 to Dec 2008]
Bachelor of Technology in Biotechnology (80.81%) [Aug 2002 to May 2006] Godavari Institute of Engineering and Technology, Rajahmundry, A.P. Intermediate Education[ M.P.C.] (91.2%) Narayana Junior College, Aseelmetta , Visakhaptanam, A.P.
[Aug 2000 to May 2002]
High School (ICSE) (83.5%) [May 2000] St.Josephs Secondary School, Malkapuram, Visakhaptanam A.P. EMPLOYMENT BIO 153 Teaching Assistant [Aug 2006 to Dec 2006] Introductory laboratory for undergraduates dealing with the various aspects of biological systems at the organismal, population and community levels Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A. Duties included general maintenance of the lab, lectures and conducting experiments and analysis of data, conducting examinations and grading. BIO151 Teaching Assistant [Jan 2007 to May 2007] Introductory laboratory for undergraduates in which biological systems are investigated at the cellular and molecular levels. Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A. Duties included general maintenance of the lab, lectures and conducting experiments and analysis of data, conducting examinations and grading.
BIO 510 Recombinant DNA Technology Lab Teaching Assistant
[Aug 2007 to Dec 2007] [Aug 2008 to Dec 2008] Advanced laboratory course for undergraduates and graduate students in construction, isolation, and analysis of recombinant DNA clones, with emphasis on practical experience in basic techniques. Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A. Duties included general maintenance of the lab, ensuring proper conduct of experiments with minimal supervision. Ordering supplies and making creative choices for the smooth operation of the course.
BIO 209 Introductory Microbiology Lab [Jan 2008 to May 2008] Introductory laboratory for undergraduates introducing fundamental microbiological principles and techniques. Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A. Duties included general maintenance of the lab, lectures and conducting experiments and analysis of data, conducting examinations and grading. RESEARCH ASSISTANT
[May 2007 to Aug 2007] [May 2008 to Aug 2008] Employer: Dr. Peter .M. Mirabito, Department Of Biological Sciences, University Of Kentucky, KY Duties included maintenance of the lab, Designing and implementing experiments, administration and inventory management, and training undergraduate students[KBRIN program] in the lab.
RESEARCH EXPERIENCE The Role of TLH genes in telomere maintenance
[Jan 2007 to Dec 2008]
The ends of linear chromosomes have evolved into specialized, multi-functional chromatin domains. These domains include the telomere proper, which typically consists of simple repetitive DNA sequences and the proteins that bind to them, and the subtelomeric region, a less well-defined domain which often includes more complex repetitive DNA elements. Telomeric repeats and their binding proteins are well conserved and their function is, in principle, understood. In contrast, subtelomeric elements vary among species and even among varieties of a single species, and their functions are unknown. We are using Aspergillus nidulans to study the function of telomere-linked helicase (TLH) genes, which are conserved, fungal-specific subtelomeric sequences. As part of our investigation to study tlhA function,multiple tlhA::Af pyrG mutants were created, each of which exhibited the same two phenotypes: slow colony growth and defective ascosporogenesis. Based on our observations we speculate that telomeric heterochromatin represses TLH and other subtelomeric genes during vegetative growth and further speculate that changes in telomere structure during ascosporogenesis underlie the enhanced TPE phenotype we observe during sexual reproduction. This work was done under the guidance of Dr. Peter M. Mirabito, Department of Biological Sciences, University of Kentucky.
Interaction of CG14011 product with HOAP protein
[Aug 2006 to Dec 2006]
Drosophila Heterochromatin protein (HP1) complex contains subunits of the Origin Recognition Complex, known to bind and recruit DNA replication factors to specific sequences in the initiation of DNA replication. The association of this heterochromatin protein with DNA replication proteins suggests a mechanism for linking the process of heterochromatin assembly to that of DNA replication. This complex contains a second previously uncharacterized DNA-binding activity that we have designated as HP1/ORC-Associated Protein (HOAP). We have found this protein to function in the assembly of a specific heterochromatic structure at chromosome ends that prevents them from undergoing end-toend fusions. A Two Hybrid Screen of cDNA library with HOAP gave a list of possible candidates of which CG14011 is a testes specific gene. To confirm the interaction of the HOAP protein with CG14011 gene product, an co precipitation assay was done using radioactively in vitro translated CG14011 gene and HOAP protein. The result of interaction between the two proteins have been conclusively demonstrated. This work was done under the guidance of Dr. Rebecca Kellum, Department of Biological Sciences, University of Kentucky. Reconstitution of Dihaploids from Leaf Tissue of Anther Derived Haploids and Molecular Characterization [January 2006-June 2006] Haploid plants have many uses in genetics and plant breeding research. There are numerous ways of producing individuals which include twin selection, wide crossing, and in vitro techniques such as anther culture, pollen culture and ovule culture, which rely on our ability to divert tissues with gametic (haploid, n) chromosome number to develop as sporophytes while retaining the haploid chromosome complement. However, haploid plants are shorter than diploids, weaker, and sterile because of univalent chromosomes. However, it happens with some haploid plants that the meiotic division takes place in macrosporogenesis and microsporogenesis and these results in spontaneous development of a fertile diploid plant. It was indicated that ‘aged’ plant portions might be useful in developing diploids by way of tissue culture techniques. Thus the problem was to locate cells, in selected haploid plants, that were capable of dividing and organizing into normal diploid plants. Several plant components were tested to determine their usefulness in production of diploids from haploids that had grown to the flowering stage as part of their selection procedure. It was found that leaf mid veins from aged leaves (those that remained on the plants 3 to 4 weeks after attaining full expansion) when cultured in chemically defined media gave rise to diploids which were normal in appearance; self fertile and produced diploid progeny. In a period of 3 months, anther derived haploids were raised and were subjected to leaf mid vein culture by optimizing media favoring development of dihaploids. A large number of haploids have been cultured to produce dihaploids. This work was done under the guidance of Dr.Sarala, Biotechnology Division, Central Tobacco Research Institute, Rajahmundry. RELATED COURSEWORK Advanced Cell Biology, Bioinformatics, Molecular genetics , Advanced genetic analysis, Biostatistics, Experimental Genetics, Quantitative Analysis, Advanced Biochemistry, Probability and Statistics, Molecular modeling and Drug Design, Microbiology, Thermodynamics, Mass Transfer Operations, Chemical reaction engineering, Bioorganic Chemistry, Biopharmaceutical Technology, Food Technology, Industrial Microbial technology, Protein Engineering, Enzyme Technology.
RELEVANT SKILLS Chemistry Basic operation and maintenance of spectrophotometers (i.e. Spec 21 and UV-vis) to monitor growth of microbial cultures and to assay enzymatic activities. Skillful in preparation of various stock solutions and reagents including anaerobic stocks.
Microbiology Preparation of various media including anaerobic to support growth of microorganisms and operation of autoclaves. Sterile technique in isolation and transfer of microorganisms. Maintenance and storage of aerobic and anaerobic microbial cultures. Basic operation and maintenance of bright field, fluorescent and phase contrast microscopes. Basic staining techniques and performing various microbiological tests like IMVAC. Preparation of various fungal media and handling of pathogenic strains of fungi.
Molecular Biology Gel Electrophoresis of DNA and RNA SDS PAGE electrophoresis techniques PCR including Inverse PCR, Real Time PCR, Reverse Transcriptase PCR, Nested PCR. RAPD and RFLP. Site Directed and Random Mutagenesis using Chemical and PCR methods. Pulse Field (CHEF) gel Electrophoresis Northern , Western and Southern Blots using radioactive and non radioactive Probes. DNA and RNA extraction from eukaryotic and prokaryotic cells including whole chromosome extraction. Achilles Heel RecA mediated cleavage of whole chromosomes. Restriction Digestion, Ligation, Cloning and Transformation techniques. Two Hybrid Colony screening. Proficient in Use of recombineering technologies like Cre-Lox, GATEWAY, TOPO cloning. Co precipitation of proteins. Expression and isolation of Tagged Proteins from Bacterial and Eukaryotic cells. Sequencing using automated sequencers and interpretation and assembly of contigs. Handling of phage and eukaryotic vectors. Proficient in FISH techniques and various other assays. Plant tissue culture. Extensive knowledge in various Bioinformatic tools and services like NCBI, Swiss PDB, EMBL, RasMol, BLAST, CLUSTALW, VectorNti.
Computer Proficiency in operation of personal computers and software packages like Microsoft Office. Use of MS Excel to create spreadsheets, graphs and statistically analyze data (i.e. averages, standard deviation, student t-test, linear regression analysis). Use of MS Access to create databases and maintain them. Literature searches through programs such as PubMed. Use of bibliography management tools like EndNote. Supervisory Skills Managed group lab sessions and evaluated a total of 180 undergraduate students over 5 semesters. Monitored activity of students and ensured safety provisions were used. Ordered lab supplies and maintained general business of the lab. ACADEMIC AWARDS “Flora G. Ribble Scholarship” for the year 2007 and 2008, Department of Biology, University of Kentucky, Lexington, Kentucky. Branch Topper in Biotechnology in Third Year Second Semester at GIET, Rajahmundry. State Rank (19) in Biotechnology from JNTU, Hyderabad. First Prize in Working Model presentation for Project Titled ‘ETHANOL PRODUCTION USING AGRICULTURAL WASTE’ at SRUJANA 2003, Hyderabad GRE Exam (1410/1600), TOEFL (290/300)
CONFERENCES AND WORKSHOPS Work shop on “Bio Informatics and tools and applications” at the Indian Institute of Technology (IIT), New Delhi. [February23-27, 2005] 47th Annual American Society of Cell Biology Meeting, Washington D.C. [December 1-5,2007] Cellular & Molecular Fungal Biology, Gordon Research Conferences, Holderness, NH. [June 29-July 3,2008] Kentucky Academy of Sciences Annual Meeting 2008, Lexington, KY. [Nov 1-Nov 3, 2008]
POSTERS Telomeres, Telomere Crisis, and Telomere-linked RecQ Helicases in Aspergillus nidulans S. Mallareddy, D.W. Perry, G. Li, M. Farman, P. M. Mirabito; 47th Annual American Society of Cell Biology Meeting, Washington D.C., December 1-5,2007. A Telomere Position Effect During Vegetative Growth and Ascosporogenesis in Aspergillus nidulans Sandeep Mallareddy, Mark Farman and Pete Mirabito; Cellular & Molecular Fungal Biology, Gordon Research Conferences, Holderness, NH. June 29-July 3,2008. Genetic Analysis of RecQ Helicase Function in Aspergillus nidulans Christopher Allen, Jordan Robinson, Kayla Kinker, Sandeep Mallareddy, and Pete Mirabito. KBRIN/INBRE Summer Undergraduate Research Program, Department of Biology, University of Kentucky, Lexington, Kentucky 40506-0225 PERSONAL SKILLS I am a highly motivated individual with good technical and management skills complimented by excellent verbal and written skills. I am a fast learner with positive attitude and self confidence and can easily adapt to new situations.
REFERENCES Dr. Peter M Mirabito, Associate Professor, Dept of Biology , UKY([email protected]) Dr. Brian Rymond, , Professor ,Director of Graduate Studies , Dept of Biology , UKY ([email protected]) Dr. Rebecca Kellum, Assistant Professor, Dept of Biology , UKY ([email protected]) Mr. John Seabolt , Course Administrator , Dept of Biology , UKY ([email protected]) Dr.P.Suryanarayana Raju, H.O.D, Biotechnology, Godavari Institute of Engineering and technology, Rajahmundry
------------------------------------------------------------------------------------------------------------------------------OBJECTIVE To apply my knowledge and experience in solving biological problems and developing sustainable and novel technologies. EDUCATION Master of Science In Biology (GPA 4.0/4.0) University of Kentucky, Lexington, Kentucky USA.
[Aug 2006 to Dec 2008]
Bachelor of Technology in Biotechnology (80.81%) [Aug 2002 to May 2006] Godavari Institute of Engineering and Technology, Rajahmundry, A.P. Intermediate Education[ M.P.C.] (91.2%) Narayana Junior College, Aseelmetta , Visakhaptanam, A.P.
[Aug 2000 to May 2002]
High School (ICSE) (83.5%) [May 2000] St.Josephs Secondary School, Malkapuram, Visakhaptanam A.P. EMPLOYMENT BIO 153 Teaching Assistant [Aug 2006 to Dec 2006] Introductory laboratory for undergraduates dealing with the various aspects of biological systems at the organismal, population and community levels Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A. Duties included general maintenance of the lab, lectures and conducting experiments and analysis of data, conducting examinations and grading. BIO151 Teaching Assistant [Jan 2007 to May 2007] Introductory laboratory for undergraduates in which biological systems are investigated at the cellular and molecular levels. Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A. Duties included general maintenance of the lab, lectures and conducting experiments and analysis of data, conducting examinations and grading.
BIO 510 Recombinant DNA Technology Lab Teaching Assistant
[Aug 2007 to Dec 2007] [Aug 2008 to Dec 2008] Advanced laboratory course for undergraduates and graduate students in construction, isolation, and analysis of recombinant DNA clones, with emphasis on practical experience in basic techniques. Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A. Duties included general maintenance of the lab, ensuring proper conduct of experiments with minimal supervision. Ordering supplies and making creative choices for the smooth operation of the course.
BIO 209 Introductory Microbiology Lab [Jan 2008 to May 2008] Introductory laboratory for undergraduates introducing fundamental microbiological principles and techniques. Employer: Department Of Biological Sciences, University Of Kentucky, KY, U.S.A. Duties included general maintenance of the lab, lectures and conducting experiments and analysis of data, conducting examinations and grading. RESEARCH ASSISTANT
[May 2007 to Aug 2007] [May 2008 to Aug 2008] Employer: Dr. Peter .M. Mirabito, Department Of Biological Sciences, University Of Kentucky, KY Duties included maintenance of the lab, Designing and implementing experiments, administration and inventory management, and training undergraduate students[KBRIN program] in the lab.
RESEARCH EXPERIENCE The Role of TLH genes in telomere maintenance
[Jan 2007 to Dec 2008]
The ends of linear chromosomes have evolved into specialized, multi-functional chromatin domains. These domains include the telomere proper, which typically consists of simple repetitive DNA sequences and the proteins that bind to them, and the subtelomeric region, a less well-defined domain which often includes more complex repetitive DNA elements. Telomeric repeats and their binding proteins are well conserved and their function is, in principle, understood. In contrast, subtelomeric elements vary among species and even among varieties of a single species, and their functions are unknown. We are using Aspergillus nidulans to study the function of telomere-linked helicase (TLH) genes, which are conserved, fungal-specific subtelomeric sequences. As part of our investigation to study tlhA function,multiple tlhA::Af pyrG mutants were created, each of which exhibited the same two phenotypes: slow colony growth and defective ascosporogenesis. Based on our observations we speculate that telomeric heterochromatin represses TLH and other subtelomeric genes during vegetative growth and further speculate that changes in telomere structure during ascosporogenesis underlie the enhanced TPE phenotype we observe during sexual reproduction. This work was done under the guidance of Dr. Peter M. Mirabito, Department of Biological Sciences, University of Kentucky.
Interaction of CG14011 product with HOAP protein
[Aug 2006 to Dec 2006]
Drosophila Heterochromatin protein (HP1) complex contains subunits of the Origin Recognition Complex, known to bind and recruit DNA replication factors to specific sequences in the initiation of DNA replication. The association of this heterochromatin protein with DNA replication proteins suggests a mechanism for linking the process of heterochromatin assembly to that of DNA replication. This complex contains a second previously uncharacterized DNA-binding activity that we have designated as HP1/ORC-Associated Protein (HOAP). We have found this protein to function in the assembly of a specific heterochromatic structure at chromosome ends that prevents them from undergoing end-toend fusions. A Two Hybrid Screen of cDNA library with HOAP gave a list of possible candidates of which CG14011 is a testes specific gene. To confirm the interaction of the HOAP protein with CG14011 gene product, an co precipitation assay was done using radioactively in vitro translated CG14011 gene and HOAP protein. The result of interaction between the two proteins have been conclusively demonstrated. This work was done under the guidance of Dr. Rebecca Kellum, Department of Biological Sciences, University of Kentucky. Reconstitution of Dihaploids from Leaf Tissue of Anther Derived Haploids and Molecular Characterization [January 2006-June 2006] Haploid plants have many uses in genetics and plant breeding research. There are numerous ways of producing individuals which include twin selection, wide crossing, and in vitro techniques such as anther culture, pollen culture and ovule culture, which rely on our ability to divert tissues with gametic (haploid, n) chromosome number to develop as sporophytes while retaining the haploid chromosome complement. However, haploid plants are shorter than diploids, weaker, and sterile because of univalent chromosomes. However, it happens with some haploid plants that the meiotic division takes place in macrosporogenesis and microsporogenesis and these results in spontaneous development of a fertile diploid plant. It was indicated that ‘aged’ plant portions might be useful in developing diploids by way of tissue culture techniques. Thus the problem was to locate cells, in selected haploid plants, that were capable of dividing and organizing into normal diploid plants. Several plant components were tested to determine their usefulness in production of diploids from haploids that had grown to the flowering stage as part of their selection procedure. It was found that leaf mid veins from aged leaves (those that remained on the plants 3 to 4 weeks after attaining full expansion) when cultured in chemically defined media gave rise to diploids which were normal in appearance; self fertile and produced diploid progeny. In a period of 3 months, anther derived haploids were raised and were subjected to leaf mid vein culture by optimizing media favoring development of dihaploids. A large number of haploids have been cultured to produce dihaploids. This work was done under the guidance of Dr.Sarala, Biotechnology Division, Central Tobacco Research Institute, Rajahmundry. RELATED COURSEWORK Advanced Cell Biology, Bioinformatics, Molecular genetics , Advanced genetic analysis, Biostatistics, Experimental Genetics, Quantitative Analysis, Advanced Biochemistry, Probability and Statistics, Molecular modeling and Drug Design, Microbiology, Thermodynamics, Mass Transfer Operations, Chemical reaction engineering, Bioorganic Chemistry, Biopharmaceutical Technology, Food Technology, Industrial Microbial technology, Protein Engineering, Enzyme Technology.
RELEVANT SKILLS Chemistry Basic operation and maintenance of spectrophotometers (i.e. Spec 21 and UV-vis) to monitor growth of microbial cultures and to assay enzymatic activities. Skillful in preparation of various stock solutions and reagents including anaerobic stocks.
Microbiology Preparation of various media including anaerobic to support growth of microorganisms and operation of autoclaves. Sterile technique in isolation and transfer of microorganisms. Maintenance and storage of aerobic and anaerobic microbial cultures. Basic operation and maintenance of bright field, fluorescent and phase contrast microscopes. Basic staining techniques and performing various microbiological tests like IMVAC. Preparation of various fungal media and handling of pathogenic strains of fungi.
Molecular Biology Gel Electrophoresis of DNA and RNA SDS PAGE electrophoresis techniques PCR including Inverse PCR, Real Time PCR, Reverse Transcriptase PCR, Nested PCR. RAPD and RFLP. Site Directed and Random Mutagenesis using Chemical and PCR methods. Pulse Field (CHEF) gel Electrophoresis Northern , Western and Southern Blots using radioactive and non radioactive Probes. DNA and RNA extraction from eukaryotic and prokaryotic cells including whole chromosome extraction. Achilles Heel RecA mediated cleavage of whole chromosomes. Restriction Digestion, Ligation, Cloning and Transformation techniques. Two Hybrid Colony screening. Proficient in Use of recombineering technologies like Cre-Lox, GATEWAY, TOPO cloning. Co precipitation of proteins. Expression and isolation of Tagged Proteins from Bacterial and Eukaryotic cells. Sequencing using automated sequencers and interpretation and assembly of contigs. Handling of phage and eukaryotic vectors. Proficient in FISH techniques and various other assays. Plant tissue culture. Extensive knowledge in various Bioinformatic tools and services like NCBI, Swiss PDB, EMBL, RasMol, BLAST, CLUSTALW, VectorNti.
Computer Proficiency in operation of personal computers and software packages like Microsoft Office. Use of MS Excel to create spreadsheets, graphs and statistically analyze data (i.e. averages, standard deviation, student t-test, linear regression analysis). Use of MS Access to create databases and maintain them. Literature searches through programs such as PubMed. Use of bibliography management tools like EndNote. Supervisory Skills Managed group lab sessions and evaluated a total of 180 undergraduate students over 5 semesters. Monitored activity of students and ensured safety provisions were used. Ordered lab supplies and maintained general business of the lab. ACADEMIC AWARDS “Flora G. Ribble Scholarship” for the year 2007 and 2008, Department of Biology, University of Kentucky, Lexington, Kentucky. Branch Topper in Biotechnology in Third Year Second Semester at GIET, Rajahmundry. State Rank (19) in Biotechnology from JNTU, Hyderabad. First Prize in Working Model presentation for Project Titled ‘ETHANOL PRODUCTION USING AGRICULTURAL WASTE’ at SRUJANA 2003, Hyderabad GRE Exam (1410/1600), TOEFL (290/300)
CONFERENCES AND WORKSHOPS Work shop on “Bio Informatics and tools and applications” at the Indian Institute of Technology (IIT), New Delhi. [February23-27, 2005] 47th Annual American Society of Cell Biology Meeting, Washington D.C. [December 1-5,2007] Cellular & Molecular Fungal Biology, Gordon Research Conferences, Holderness, NH. [June 29-July 3,2008] Kentucky Academy of Sciences Annual Meeting 2008, Lexington, KY. [Nov 1-Nov 3, 2008]
POSTERS Telomeres, Telomere Crisis, and Telomere-linked RecQ Helicases in Aspergillus nidulans S. Mallareddy, D.W. Perry, G. Li, M. Farman, P. M. Mirabito; 47th Annual American Society of Cell Biology Meeting, Washington D.C., December 1-5,2007. A Telomere Position Effect During Vegetative Growth and Ascosporogenesis in Aspergillus nidulans Sandeep Mallareddy, Mark Farman and Pete Mirabito; Cellular & Molecular Fungal Biology, Gordon Research Conferences, Holderness, NH. June 29-July 3,2008. Genetic Analysis of RecQ Helicase Function in Aspergillus nidulans Christopher Allen, Jordan Robinson, Kayla Kinker, Sandeep Mallareddy, and Pete Mirabito. KBRIN/INBRE Summer Undergraduate Research Program, Department of Biology, University of Kentucky, Lexington, Kentucky 40506-0225 PERSONAL SKILLS I am a highly motivated individual with good technical and management skills complimented by excellent verbal and written skills. I am a fast learner with positive attitude and self confidence and can easily adapt to new situations.
REFERENCES Dr. Peter M Mirabito, Associate Professor, Dept of Biology , UKY([email protected]) Dr. Brian Rymond, , Professor ,Director of Graduate Studies , Dept of Biology , UKY ([email protected]) Dr. Rebecca Kellum, Assistant Professor, Dept of Biology , UKY ([email protected]) Mr. John Seabolt , Course Administrator , Dept of Biology , UKY ([email protected]) Dr.P.Suryanarayana Raju, H.O.D, Biotechnology, Godavari Institute of Engineering and technology, Rajahmundry
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