Basic Study Of Heavy Metals In Tilapia (2008)
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Project 5.2.1
Basic study of Heavy metals in Tilapia
Assessment of heavy metals in fresh water fish and selected marine fish
B.K.K.K.Jinadasa & R. Edirisinghe, Research Officer, Post Harvest Technology Division, NARA
Objectives:
Sampling: • Sample taken from Tilapia sp.
Sampling location:
Anuradapura District:
Padaviya
Anuradapura
Polonnaruwa
Ampara
Hambantota
Wilachchiya Koobichchiyankulama Kalawewa Rajanganaya Thuruwila
Polonnaruwa District: Koobichchiyankulama
Wilachchiya
Padaviya Minneriya Girithale Parakrama Samudraya Thuruwila Kaudulla Maduruoya Rajanganaya
Kalawewa
Ampara District: Parakrama Samudraya Nawagiriyawa Minneriya Girithale
Senanayake samudraya
Ambalanoya
Kaudulla
Maduru oya
Hambantota District: Ambalanoya
Udawalawa
Senanayake Samudraya Udukiriwila
Nawagiriyawa
Mahagalwewa
Lunugamwehera
Methodology:
Udawalawa
Udukiriwila
Lunugamwehera
Mahagalwewa
Calculation:
Database: As‐reading 1.1604 0.9753 0.9636 0.8207 0.6621 2.7033 0.6033 0.6221 0.6206 0.5633 0.5099 0.3519 0.4590 0.4569 0.3988 0.6824 0.2490 0.5294 0.6206 0.0614 0.3366 0.3345 0.3898 0.2769 0.8320 0.4912
Cd‐conc (ppb) Pb conc (ppb) Hg conc (ppb) As conc (ppb) 85.1845 49.7467 162.5000 209.9132 3.1897 295.8041 147.3324 185.1718 230.3807 4408.1691 172.6421 184.3505 129.9245 135.7625 112.3312 162.9666 15.9207 303.8084 78.9164 129.9764 100.2142 3163.7266 71.4369 526.3435 72.3699 75.8960 143.1599 116.2428 8.2789 372.7578 54.9692 115.9985 127.8673 3554.9573 108.8341 117.6493 0.0000 0.0000 58.8593 100.7152 1.3657 455.0045 45.5705 91.6262 0.1391 489.7607 216.6945 97.9132 3.2332 0.0000 47.9840 87.2955 4.4337 258.4863 42.8472 79.1306 0.3046 0.0000 165.0676 75.9185 46.2653 0.0000 83.9651 126.7930 183.5912 545.0048 46.3992 47.5645 1.4246 1938.0491 71.6858 104.7487 46.3830 0.0000 81.9923 120.0387 64.6263 235.9148 74.9408 10.3821 355.4598 5072.4138 433.3333 483.6207 2.2192 0.0000 92.1550 65.1158 3.9417 780.1528 13.8739 67.6854 8.1073 695.7635 132.9778 84.3950 130.3101 0.0000 50.2907 161.2403 0.0000 544.2388 2.1366 93.7047
Cd‐conc (mgPb conc (mgHg conc (mgAs conc (mg 0.0852 0.0497 0.1625 0.2099 0.0032 0.2958 0.1473 0.1852 0.2304 4.4082 0.1726 0.1844 0.1299 0.1358 0.1123 0.1630 0.0159 0.3038 0.0789 0.1300 0.1002 3.1637 0.0714 0.5263 0.0724 0.0759 0.1432 0.1162 0.0083 0.3728 0.0550 0.1160 0.1279 3.5550 0.1088 0.1176 0.0000 0.0000 0.0589 0.1007 0.0014 0.4550 0.0456 0.0916 0.0001 0.4898 0.2167 0.0979 0.0032 0.0000 0.0480 0.0873 0.0044 0.2585 0.0428 0.0791 0.0003 0.0000 0.1651 0.0759 0.0463 0.0000 0.0840 0.1268 0.1836 0.5450 0.0464 0.0476 0.0014 1.9380 0.0717 0.1047 0.0464 0.0000 0.0820 0.1200 0.0646 0.2359 0.0749 0.0104 0.3555 5.0724 0.4333 0.4836 0.0022 0.0000 0.0922 0.0651 0.0039 0.7802 0.0139 0.0677 0.0081 0.6958 0.1330 0.0844 0.1303 0.0000 0.0503 0.1612 0.0000 0.5442 0.0021 0.0937
Length‐Weight Data
Results & Discussion:
Length Classes Mean length (cm) Mean weight (g)
District
Cd
Pb
Hg
Gills
As
Cd
Pb
Class A (<17.9)
16.7
102.11
75
Class B (18‐19.9)
18.7
141.20
86
Class C (20‐21.9)
20.8
193.17
83
Class D (22‐23.9)
22.8
242.20
61
Class E (>24)
27.5
440.66
69
Total Number of samples
374
Mean concentration of Total Hg, As, Cd and Pb (mg/Kg dry wt.) in the muscle, gill and liver tissues of Tilapia sp. in four districts.
Heavy Metal Data (District‐vice)‐mg/Kg dry wt Flesh
Liver
Hg
As
Cd
Pb
Hg
As
A’puraya 0.0463 0.0245 0.1436 0.2655 0.0406 0.9753 0.0495 0.2770 0.2511 2.2207 0.1662 0.3880
Po’ruwa
0.0205 0.0218 0.2393 0.2899 0.0133 0.1323 0.3969 0.2816 0.2821 0.9007 0.6164 0.4564
Ampara
0.0198 0.0768 0.4592 0.2852 0.0086 0.0173 0.6233 0.2925 0.2466 1.5092 1.2639 0.2931
H’thota
0.0404 0.1248 0.3377 0.2041 0.0173 0.3132 0.5853 0.1665 0.3892 3.3022 0.5182 0.2438
Permissible levels:
Conclusion:
(Levels in mg/Kg, wet wt basis)
Cd
Pb
As
Hg
EU-
0.05
0.2
_
0.5
FSANZ
_
0.5
_
1.0
WHO/FAO committee on food additives (JECFA)
_
3.6
0.12
_
GOV of SLNo.1528/7 Export regulation
0.05
0.2
_
0.5
EC No 78/2005
n
Follow-up action:
THANK YOU!!!!!
Basic study of Heavy metals in Tilapia
Assessment of heavy metals in fresh water fish and selected marine fish
B.K.K.K.Jinadasa & R. Edirisinghe, Research Officer, Post Harvest Technology Division, NARA
Objectives:
Sampling: • Sample taken from Tilapia sp.
Sampling location:
Anuradapura District:
Padaviya
Anuradapura
Polonnaruwa
Ampara
Hambantota
Wilachchiya Koobichchiyankulama Kalawewa Rajanganaya Thuruwila
Polonnaruwa District: Koobichchiyankulama
Wilachchiya
Padaviya Minneriya Girithale Parakrama Samudraya Thuruwila Kaudulla Maduruoya Rajanganaya
Kalawewa
Ampara District: Parakrama Samudraya Nawagiriyawa Minneriya Girithale
Senanayake samudraya
Ambalanoya
Kaudulla
Maduru oya
Hambantota District: Ambalanoya
Udawalawa
Senanayake Samudraya Udukiriwila
Nawagiriyawa
Mahagalwewa
Lunugamwehera
Methodology:
Udawalawa
Udukiriwila
Lunugamwehera
Mahagalwewa
Calculation:
Database: As‐reading 1.1604 0.9753 0.9636 0.8207 0.6621 2.7033 0.6033 0.6221 0.6206 0.5633 0.5099 0.3519 0.4590 0.4569 0.3988 0.6824 0.2490 0.5294 0.6206 0.0614 0.3366 0.3345 0.3898 0.2769 0.8320 0.4912
Cd‐conc (ppb) Pb conc (ppb) Hg conc (ppb) As conc (ppb) 85.1845 49.7467 162.5000 209.9132 3.1897 295.8041 147.3324 185.1718 230.3807 4408.1691 172.6421 184.3505 129.9245 135.7625 112.3312 162.9666 15.9207 303.8084 78.9164 129.9764 100.2142 3163.7266 71.4369 526.3435 72.3699 75.8960 143.1599 116.2428 8.2789 372.7578 54.9692 115.9985 127.8673 3554.9573 108.8341 117.6493 0.0000 0.0000 58.8593 100.7152 1.3657 455.0045 45.5705 91.6262 0.1391 489.7607 216.6945 97.9132 3.2332 0.0000 47.9840 87.2955 4.4337 258.4863 42.8472 79.1306 0.3046 0.0000 165.0676 75.9185 46.2653 0.0000 83.9651 126.7930 183.5912 545.0048 46.3992 47.5645 1.4246 1938.0491 71.6858 104.7487 46.3830 0.0000 81.9923 120.0387 64.6263 235.9148 74.9408 10.3821 355.4598 5072.4138 433.3333 483.6207 2.2192 0.0000 92.1550 65.1158 3.9417 780.1528 13.8739 67.6854 8.1073 695.7635 132.9778 84.3950 130.3101 0.0000 50.2907 161.2403 0.0000 544.2388 2.1366 93.7047
Cd‐conc (mgPb conc (mgHg conc (mgAs conc (mg 0.0852 0.0497 0.1625 0.2099 0.0032 0.2958 0.1473 0.1852 0.2304 4.4082 0.1726 0.1844 0.1299 0.1358 0.1123 0.1630 0.0159 0.3038 0.0789 0.1300 0.1002 3.1637 0.0714 0.5263 0.0724 0.0759 0.1432 0.1162 0.0083 0.3728 0.0550 0.1160 0.1279 3.5550 0.1088 0.1176 0.0000 0.0000 0.0589 0.1007 0.0014 0.4550 0.0456 0.0916 0.0001 0.4898 0.2167 0.0979 0.0032 0.0000 0.0480 0.0873 0.0044 0.2585 0.0428 0.0791 0.0003 0.0000 0.1651 0.0759 0.0463 0.0000 0.0840 0.1268 0.1836 0.5450 0.0464 0.0476 0.0014 1.9380 0.0717 0.1047 0.0464 0.0000 0.0820 0.1200 0.0646 0.2359 0.0749 0.0104 0.3555 5.0724 0.4333 0.4836 0.0022 0.0000 0.0922 0.0651 0.0039 0.7802 0.0139 0.0677 0.0081 0.6958 0.1330 0.0844 0.1303 0.0000 0.0503 0.1612 0.0000 0.5442 0.0021 0.0937
Length‐Weight Data
Results & Discussion:
Length Classes Mean length (cm) Mean weight (g)
District
Cd
Pb
Hg
Gills
As
Cd
Pb
Class A (<17.9)
16.7
102.11
75
Class B (18‐19.9)
18.7
141.20
86
Class C (20‐21.9)
20.8
193.17
83
Class D (22‐23.9)
22.8
242.20
61
Class E (>24)
27.5
440.66
69
Total Number of samples
374
Mean concentration of Total Hg, As, Cd and Pb (mg/Kg dry wt.) in the muscle, gill and liver tissues of Tilapia sp. in four districts.
Heavy Metal Data (District‐vice)‐mg/Kg dry wt Flesh
Liver
Hg
As
Cd
Pb
Hg
As
A’puraya 0.0463 0.0245 0.1436 0.2655 0.0406 0.9753 0.0495 0.2770 0.2511 2.2207 0.1662 0.3880
Po’ruwa
0.0205 0.0218 0.2393 0.2899 0.0133 0.1323 0.3969 0.2816 0.2821 0.9007 0.6164 0.4564
Ampara
0.0198 0.0768 0.4592 0.2852 0.0086 0.0173 0.6233 0.2925 0.2466 1.5092 1.2639 0.2931
H’thota
0.0404 0.1248 0.3377 0.2041 0.0173 0.3132 0.5853 0.1665 0.3892 3.3022 0.5182 0.2438
Permissible levels:
Conclusion:
(Levels in mg/Kg, wet wt basis)
Cd
Pb
As
Hg
EU-
0.05
0.2
_
0.5
FSANZ
_
0.5
_
1.0
WHO/FAO committee on food additives (JECFA)
_
3.6
0.12
_
GOV of SLNo.1528/7 Export regulation
0.05
0.2
_
0.5
EC No 78/2005
n
Follow-up action:
THANK YOU!!!!!
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