Bahan Kuliah 03a-virus Terbawa Benih Sawi

  • May 2020
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Chrysantemum Virus-B infeksi bersifat sistemik bibit berupa anakan dapat terserang

Colour break Sumber: Dr. Ir. Sri Hendrastuti

Mosaik Sumber: Dr. Ir. Sri Hendrastuti

A

D

B

E

C

F

Sumber: Dr.Ir Sri Hendrastuti

Variasi gejala Turnip Mosaic Virus pada beberapa tanaman. A. Mosaik pada caisin, B. Daun mengerut pada caisin C. lesio lokal pada Chenopodium amaranticolor D. Vein banding (penebalan warna tulang daun) pada lobak E. Vein clearing (pemucatan tulang daun) pada caisin F. Proliferasi pada caisin.0

0.45

Nila Absorban (405 nm)

0.4 0.35 0.3 0.25 0.2 0.15 0.1 0.05 0 KP

KN

Bufer

TP

1:30

1:50

1:100

1:1000

Faktor Pengenceran Sampel Ekstrak Biji

Ekstrak Kecambah

Hasil pengujian biji dan kecambah sawi hijau dengan teknik DAS-ELISA; KP: kontrol positif, KN: kontrol negatif, TP: tanpa pengenceran (Sumber Dr.Ir. Sri Hendrastuti)

Things to consider when choosing detection method • Sensitivity • Accuracy & reproducibility • Number of samples that can be processed in a given time • Cost & sophistication of the apparatus & materials • The degree of operator training required • Adaptibility to field condition

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