B1
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Graded-Dose Response Curve SECTION A Paracetamol Preparation: Doses:
Foot Volume
500 mg tablet 0.5 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0.01
0.01 0.01 0.01 0 0.01 0.01 0 0.01
0 0.01 0.01 0 0 0 0.01 0
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0.01 0 0 0 0 0 0
0.01 0.01 0.01 0 0.01 0 0.01 0.01
0 0 0.01 0.01 0.01 0.01 0 0.01
5 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0.01 0 0 0.01 0
0 0 0.01 0.01 0 0 0.01 0
0 0 0.01 0 0 0 0.01 0
16 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0.01 0 0.01 0 0.01 0.01 0.01
0.01 0 0.01 0 0.01 0.01 0.01 0
1.6 mg / 200 gm rat
ON A 3 hrs 0 0.01 0.01 0.01 0.01 0.01 0.01 0
0.01 0 0.01 0 0.01 0.01 0 0.01
0 0.01 0.01 0.01 0.01 0 0 0.01
0.01 0.01 0.01 0.01 0.01 0 0 0.01
Graded-Dose Response Curve SECTION B Aspirin Preparation: Doses:
Foot Volume
200 mg tablet 0.5 mg / 200 gm rat Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
Before
Carageenan
1 hr
2 hrs
3 hrs
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0.01 0
0 0.01 0 0 0 0.01 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
1.6 mg / 200 gm rat Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0 0.01 0.01 0 0.01
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
5 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0.01 0.01 0
0 0 0 0 0 0 0.01 0.01
0 0 0 0 0.01 0 0.01 0
0 0 0 0 0.01 0 0 0
0 0 0 0 0 0 0 0
16 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0.01 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0.01 0 0.01
Graded-Dose Response Curve SECTION C Celecoxib
Foot Volume
Preparation:200 mg tablet Doses: 0.3 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0.01 0 0.01 0 0 0.01
0.01 0.01 0 0 0 0 0.01
0 0 0 0.01 0 0 0
0 0.01 0 0.01 0 0 0
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0.01 0 0 0.01 0 0
0.01 0 0 0.01 0.01 0.01 0.01 0.01
0 0 0 0.01 0.01 0 0 0
0 0 0.01 0 0 0.01 0 0
0.01 0 0 0.01 0 0.01 0 0
3 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0.01 0 0.01 0 0 0.01 0.01
0.01 0.01 0.01 0.01 0.01 0.01 0.01 0
0 0.01 0.01 0 0 0 0.01 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
10 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0.01 0.01 0.01 0.01 0.01 0.01
0.01 0.01 0.01 0.01 0 0.01 0.01 0.01
0.01 0.01 0.01 0.01 0.01 0 0.01 0
0.01 0 0 0 0 0 0 0
0 0 0 0.01 0 0 0 0.01
1 mg / 200 gm rat
Graded-Dose Response Curve SECTION D Prednisone
Foot Volume
Preparation: 20 mg tablet Doses: 0.02 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0.01 0 0.01 0 0 0 0.01
0.01 0.01 0.01 0.01 0.01 0.01 0.01 0
0 0 0 0 0 0 0 0
0.01 0 0.01 0 0.01 0 0 0
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0.01 0 0.01 0.01 0.01 0 0
0.01 0.01 0 0 0.01 0 0.01 0.01
0 0 0 0 0 0.01 0.01 0.01
0.01 0 0.01 0 0.01 0 0 0
0.2 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0 0.01 0 0.01 0
0 0 0.01 0.01 0 0.01 0.01 0.01
0.01 0.01 0 0 0 0 0 0
0.01 0 0 0 0 0.01 0 0
0.6 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0.01 0 0 0 0.01 0.01 0 0
0 0.01 0.01 0 0.01 0.01 0.01 0.01
0.01 0.01 0 0.01 0.01 0.01 0 0
0.01 0 0 0.01 0 0.01 0 0
0.06 mg / 200 gm rat
Graded-Dose Response Curve SECTION A Paracetamol
Foot Volume
Preparation:500 mg tablet Doses: 0.5 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0.01 0 0 80
0.01 0.01 0.01 0 0.01 0.01 0 0.01 0.01 0 173.3
0 0.01 0.01 0 0 0 0.01 0 0 0 126.7
0 0.01 0.01 0.01 0.01 0.01 0.01 0 0.01 0 180.0
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0.01 0 0 0 0 0 0 0 0 100
0.01 0.01 0.01 0 0.01 0 0.01 0.01 0.01 0 192.9
0 0 0.01 0.01 0.01 0.01 0 0.01 0 0 142.9
0.01 0 0.01 0 0.01 0.01 0 0.01 0.01 0 192.9
5 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0.01 0 0 0.01 0 0 0 188.9
0 0 0.01 0.01 0 0 0.01 0 0 0 255.6
0 0 0.01 0 0 0 0.01 0 0 0 255.6
0 0.01 0.01 0.01 0.01 0 0 0.01 0 0 322.2
16 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 110.0
0 0.01 0 0.01 0 0.01 0.01 0.01 0 0 280.0
0.01 0 0.01 0 0.01 0.01 0.01 0 0.01 0 320.0
0.01 0.01 0.01 0.01 0.01 0 0 0.01 0 0 290.0
1.6 mg / 200 gm rat
Graded-Dose Response Curve SECTION B Aspirin
Foot Volume
Preparation:200 mg tablet Doses: 0.5 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0.01 0 0 0 38.5
0 0.01 0 0 0 0.01 0 0 0 0 84.6
0 0 0 0 0 0 0 0 0 0 -38.5
0 0 0 0 0 0 0 0 0 0 23.1
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0.01 0.01 0 0.01 0 0 29.2
0 0 0 0 0 0 0 0 0 0 -20.8
0 0 0 0 0 0 0 0 0 0 -20.8
0 0 0 0 0 0 0 0 0 0 -33.3
5 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0.01 0.01 0 0
0 0 0 0 0 0 0.01 0.01 0 0 7.4
0 0 0 0 0.01 0 0.01 0 0 0 -14.8
0 0 0 0 0.01 0 0 0 0 0 -22.2
0 0 0 0 0 0 0 0 0 0 -48.1
16 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 -33.3
0 0.01 0 0 0 0 0 0 0 0 13.3
0 0 0 0 0 0 0 0 0 0 6.7
0 0 0 0 0 0.01 0 0.01 0 0 33.3
1.6 mg / 200 gm rat
Graded-Dose Response Curve SECTION C Celecoxib
Foot Volume
Preparation:200 mg tablet Doses: 0.3 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0
0.01
0
0
0.01 0 0.01 0 0 0.01 0 0 115.4
0.01 0 0 0 0 0.01 0 0 130.8
0 0 0.01 0 0 0 0 0 76.9
0.01 0 0.01 0 0 0 0 0 84.6
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0.01 0 0 0.01 0 0 0
0.01 0 0 0.01 0.01 0.01 0.01 0.01 0.01 0 55.2
0 0 0 0.01 0.01 0 0 0 0 0 3.4
0 0 0.01 0 0 0.01 0 0 0 0 -34.5
0.01 0 0 0.01 0 0.01 0 0 0 0 3.4
3 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0.01 0 0.01 0 0 0.01 0.01 0
0.01 0.01 0.01 0.01 0.01 0.01 0.01 0 0.01 0 51.6
0 0.01 0.01 0 0 0 0.01 0 0 0 16.1
0 0 0 0 0 0 0 0 0 0 -12.9
0 0 0 0 0 0 0 0 0 0 -41.9
10 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference
0 0 0.01 0.01 0.01 0.01 0.01 0.01 0.01
0.01 0.01 0.01 0.01 0 0.01 0.01 0.01 0.01 0
0.01 0.01 0.01 0.01 0.01 0 0.01 0 0 0
0.01 0 0 0 0 0 0 0 0 0
0 0 0 0.01 0 0 0 0.01 0 0
1 mg / 200 gm rat
% Difference
4.9
-7.3
-39.0
-31.7
Graded-Dose Response Curve SECTION D Prednisone
Foot Volume
Preparation:20 mg tablet Doses: 0.02 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0.01 0 0.01 0 0 0 0.01 0 0 38.1
0.01 0.01 0.01 0.01 0.01 0.01 0.01 0 0 0 90.5
0 0 0 0 0 0 0 0 0 0 -4.8
0.01 0 0.01 0 0.01 0 0 0 0 0 23.8
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0.01 0 0.01 0.01 0.01 0 0 0 0 82.4
0.01 0.01 0 0 0.01 0 0.01 0.01 0 0 105.9
0 0 0 0 0 0.01 0.01 0.01 0 0 70.6
0.01 0 0.01 0 0.01 0 0 0 0 0 76.5
0.2 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0.01 0 0.01 0 0 0 93.8
0 0 0.01 0.01 0 0.01 0.01 0.01 0 0 93.8
0.01 0.01 0 0 0 0 0 0 0 0 112.5
0.01 0 0 0 0 0.01 0 0 0 0 62.5
0.6 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean
0 0 0 0 0 0 0 0 0
0.01 0 0 0 0.01 0.01 0 0 0
0 0.01 0.01 0 0.01 0.01 0.01 0.01 0
0.01 0.01 0 0.01 0.01 0.01 0 0 0
0.01 0 0 0.01 0 0.01 0 0 0
0.06 mg / 200 gm rat
Difference % Difference
0 78.9
0 94.7
0 94.7
0 78.9
0
time Column H
Column G
Column F
Column E
Column D
foot volume
PARACETAMOL
0.01
0
0
0
0 Row 1 Row 2 Row 3 Row 5
foot volume 0 0 0
time
Column H
Column G
Column F
Column E
Column D
ASPIRIN
0
0 0
0 0 0
Row 6 Row 8 Row 9 Row 1
0
time
Column H
Column G
Column F
Column E
Column D
foot volume
CELECOXIB
0.01
0.01
0
0
0
0 Row 1 Row 1 Row 1 Row 1
PREDNISONE
time
Column H
Column G
Column F
Row 179 Row 192 Row 204 Row 216 Column E
Column D
foot volume
0.01 0 0 0 0 0 0 0 0 0 0
Column H
Row 14 Row 27 Row 39 Row 51
Column H
Row 69 Row 82 Row 94 Row 106
Column H
Row 124 Row 137 Row 149 Row 161
Column H
Row 179 Row 192 Row 204 Row 216
A
B
C
D
Dosage (mg) log (mg) After Carrageenan 0.5 -0.3 80.0 1.6 0.2 100.0 5 0.7 188.9 16 1.2 110.0 0.5 -0.3 38.5 1.6 0.2 29.2 5 0.7 7.4 16 1.2 -33.3 0.3 -0.52 115.4 1 0 55.2 3 0.48 51.6 10.0 1 4.9 0.02 -1.7 38.1 0.06 -1.22 82.4 0.2 -0.7 93.8 0.6 -0.22 78.9
% Response 1st hour
2nd hour 173.3 192.9 255.6 280.0 84.6 -20.8 -14.8 13.3 130.8 3.4 16.1 -7.3 90.5 105.9 93.8 94.7
126.7 142.9 255.6 320.0 -38.5 -20.8 -22.2 6.7 76.9 -34.5 -12.9 -39.0 -4.8 70.6 112.5 94.7
Paracetamol 325.0 300.0
250.0 225.0
Column D
200.0
Column E Column F
175.0
Column G
150.0 125.0 100.0 75.0 0
2
4
6
8
10
12
14
16
Dosage (mg)
Aspirin
% Response
C
% Response
275.0
90.0 80.0 70.0 60.0 50.0 40.0 30.0 20.0 10.0 0.0 -10.0
Column D Column E Column F Column G
% Response
60.0 50.0 40.0
Column D
30.0 20.0 10.0 0.0 -10.0 -20.0 -30.0 -40.0 -50.0
Column E Column F Column G
0
2
4
6
8
10
12
14
16
Dosage (mg)
Celecoxib 140.0 120.0
% Response
100.0 80.0 60.0
Column D
40.0
Column E Column F
20.0
Column G
0.0 -20.0 -40.0 -60.0 0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Dosage (mg)
Prednisone 120.0 110.0
% Response
100.0 90.0 80.0 70.0
Column D
60.0
Column E
50.0
Column F
40.0
Column G
30.0 20.0 10.0 0.0 -10.0 0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
0.5
0.55
0.6
% Re
50.0
Column F
40.0
Column G
30.0 20.0 10.0 0.0 -10.0 0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
0.5
0.55
0.6
Dosage (mg)
Paracetamol 325.0
% Response
300.0 275.0 250.0 225.0
Column D
200.0
Column E
175.0
Column F
150.0
Column G
125.0 100.0 75.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
log of dosage
% Response
Aspirin 90.0 80.0 70.0 60.0 50.0 40.0 30.0 20.0 10.0 0.0 -10.0 -20.0 -30.0 -40.0 -50.0 0.4 0.3 0.2 0.1
Column D Column E Column F Column G
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
log of dosage
1
1.1 1.2 1.3
-40.0 -50.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
log of dosage
Celecoxib 140.0 120.0
% Response
100.0 80.0 60.0
Column D
40.0
Column E Column F
20.0
Column G
0.0 -20.0 -40.0 -60.0 -0.75
-0.5
-0.25
0
0.25
0.5
0.75
1
log of dosage
Prednisone 120.0 110.0
% Response
100.0 90.0 80.0 70.0
Column D
60.0
Column E
50.0
Column F
40.0
Column G
30.0 20.0 10.0 0.0 -10.0 -1.8
-1.6
-1.4
-1.2
-1
-0.8
log of dosage
-0.6
-0.4
-0.2
3rd hour 180.0 192.9 322.2 290.0 23.1 -33.3 -48.1 33.3 84.6 3.4 -41.9 -31.7 23.8 76.5 62.5 78.9 B
After Carageenan injection 190.0 180.0
Column D Column E Column F Column G D
% Response
170.0 160.0 150.0 140.0 130.0 120.0 110.0 100.0 90.0 80.0 70.0 0.4 0.3 0.2 0.1
16
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Dosage (mg)
After 1 hour 280.0 270.0
Column D Column E Column F Column G
% Response
260.0 250.0 240.0 230.0 220.0 210.0 200.0
1
1.1 1.2 1.3
.55
Column F Column G
% Respons
Column E
240.0 230.0 220.0 210.0 200.0 190.0 180.0 170.0 0.4 0.3 0.2 0.1
16
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
1
1.1 1.2 1.3
1
1.1 1.2 1.3
Dosage (mg)
After 2 hours 320.0
Column D Column E Column F Column G
% Response
300.0 280.0 260.0 240.0 220.0 200.0 180.0 160.0 140.0 120.0 0.4 0.3 0.2 0.1
10.0
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Dosage (mg)
After 3 hours
Column D Column E Column F Column G
0.6
% Response
0
Column D
250.0
330.0 320.0 310.0 300.0 290.0 280.0 270.0 260.0 250.0 240.0 230.0 220.0 210.0 200.0 190.0 180.0 170.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Column G
.55
0.6
% Re
Column F
240.0 230.0 220.0 210.0 200.0 190.0 180.0 170.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Dosage (mg)
Column D Column E Column F Column G
1.2 1.3
Column D Column E Column F Column G
1.2 1.3
1
1.1 1.2 1.3
1.2 1.3
Column D Column E Column F Column G
1
Column D Column E Column F Column G
-0.2
jection
Column D
0.9
1
1.1 1.2 1.3
Column E
Column E
0.9
1
1.1 1.2 1.3
Column F
0.9
1
1.1 1.2 1.3
Column G
0.9
1
1.1 1.2 1.3
Column G
0.9
1
1.1 1.2 1.3
A
B
C
% Inhibition of Inflammation 1st hour 2nd hour -173.3 -126.7 -192.9 -142.9 -255.6 -255.6 -280.0 -320.0 -84.6 38.5 20.8 20.8 14.8 22.2 -13.3 -6.7 -130.8 -76.9 -3.4 34.5 -16.1 12.9 7.3 39.0 -90.5 4.8 -105.9 -70.6 -93.8 -112.5 -94.7 -94.7
After Carageenan injection -80.0 -90.0 -100.0 -110.0 -120.0 -130.0 -140.0
Column D
-150.0 -160.0 -170.0 -180.0 -190.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
log of dosage
ibition of inflammation
C
% inhibition of inflammation
D
Dosage (mg) log (mg) After Carrageenan 0.5 -0.3 -80.0 1.6 0.2 -100.0 5 0.7 -188.9 16 1.2 -110.0 0.5 -0.3 -38.5 1.6 0.2 -29.2 5 0.7 -7.4 16 1.2 33.3 0.3 -0.52 -115.4 1 0 -55.2 3 0.48 -51.6 10.0 1 -4.9 0.02 -1.7 -38.1 0.06 -1.22 -82.4 0.2 -0.7 -93.8 0.6 -0.22 -78.9
After 1 hour -170.0 -180.0 -190.0 -200.0 -210.0 -220.0 -230.0 -240.0 -250.0 -260.0
Column E
% inhibition of inf
-210.0 -220.0 -230.0
Column E
-240.0 -250.0 -260.0 -270.0 -280.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
% inhibition of inflammation
log of dosage
After 2 hours -120.0 -140.0 -160.0 -180.0 -200.0 -220.0
Column F
-240.0 -260.0 -280.0 -300.0 -320.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
% inhibition of inflammation
log of dosage
After 3 hours -170.0 -180.0 -190.0 -200.0 -210.0 -220.0 -230.0 -240.0 -250.0 -260.0 -270.0 -280.0 -290.0 -300.0 -310.0 -320.0 -330.0 0.4 0.3 0.2 0.1
Column G
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
log of dosage
1
1.1 1.2 1.3
% inhibi
-300.0 -310.0 -320.0 -330.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
log of dosage
1
1.1 1.2 1.3
on 3rd hour -180.0 -192.9 -322.2 -290.0 -23.1 33.3 48.1 -33.3 -84.6 -3.4 41.9 31.7 -23.8 -76.5 -62.5 -78.9
80.0 100.0 188.9 110.0 38.5 29.2 7.4 -33.3 115.4 55.2 51.6 4.9 38.1 82.4 93.8 78.9 B
n
Column D
D
1 1.2 1.3
Column E
173.3 192.9 255.6 280.0 84.6 -20.8 -14.8 13.3 130.8 3.4 16.1 -7.3 90.5 105.9 93.8 94.7
126.7 142.9 255.6 320.0 -38.5 -20.8 -22.2 6.7 76.9 -34.5 -12.9 -39.0 -4.8 70.6 112.5 94.7
180.0 192.9 322.2 290.0 23.1 -33.3 -48.1 33.3 84.6 3.4 -41.9 -31.7 23.8 76.5 62.5 78.9
Column E
.1 1.2 1.3
Column F
.1 1.2 1.3
1 1.2 1.3
Column G
1 1.2 1.3
Foot Volume
500 mg tablet 0.5 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0.01
0.01 0.01 0.01 0 0.01 0.01 0 0.01
0 0.01 0.01 0 0 0 0.01 0
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0.01 0 0 0 0 0 0
0.01 0.01 0.01 0 0.01 0 0.01 0.01
0 0 0.01 0.01 0.01 0.01 0 0.01
5 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0.01 0 0 0.01 0
0 0 0.01 0.01 0 0 0.01 0
0 0 0.01 0 0 0 0.01 0
16 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0.01 0 0.01 0 0.01 0.01 0.01
0.01 0 0.01 0 0.01 0.01 0.01 0
1.6 mg / 200 gm rat
ON A 3 hrs 0 0.01 0.01 0.01 0.01 0.01 0.01 0
0.01 0 0.01 0 0.01 0.01 0 0.01
0 0.01 0.01 0.01 0.01 0 0 0.01
0.01 0.01 0.01 0.01 0.01 0 0 0.01
Graded-Dose Response Curve SECTION B Aspirin Preparation: Doses:
Foot Volume
200 mg tablet 0.5 mg / 200 gm rat Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
Before
Carageenan
1 hr
2 hrs
3 hrs
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0.01 0
0 0.01 0 0 0 0.01 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
1.6 mg / 200 gm rat Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0 0.01 0.01 0 0.01
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
5 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0.01 0.01 0
0 0 0 0 0 0 0.01 0.01
0 0 0 0 0.01 0 0.01 0
0 0 0 0 0.01 0 0 0
0 0 0 0 0 0 0 0
16 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0.01 0 0 0 0 0 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0.01 0 0.01
Graded-Dose Response Curve SECTION C Celecoxib
Foot Volume
Preparation:200 mg tablet Doses: 0.3 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0.01 0 0.01 0 0 0.01
0.01 0.01 0 0 0 0 0.01
0 0 0 0.01 0 0 0
0 0.01 0 0.01 0 0 0
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0.01 0 0 0.01 0 0
0.01 0 0 0.01 0.01 0.01 0.01 0.01
0 0 0 0.01 0.01 0 0 0
0 0 0.01 0 0 0.01 0 0
0.01 0 0 0.01 0 0.01 0 0
3 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0.01 0 0.01 0 0 0.01 0.01
0.01 0.01 0.01 0.01 0.01 0.01 0.01 0
0 0.01 0.01 0 0 0 0.01 0
0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0
10 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0.01 0.01 0.01 0.01 0.01 0.01
0.01 0.01 0.01 0.01 0 0.01 0.01 0.01
0.01 0.01 0.01 0.01 0.01 0 0.01 0
0.01 0 0 0 0 0 0 0
0 0 0 0.01 0 0 0 0.01
1 mg / 200 gm rat
Graded-Dose Response Curve SECTION D Prednisone
Foot Volume
Preparation: 20 mg tablet Doses: 0.02 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0.01 0 0.01 0 0 0 0.01
0.01 0.01 0.01 0.01 0.01 0.01 0.01 0
0 0 0 0 0 0 0 0
0.01 0 0.01 0 0.01 0 0 0
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0.01 0 0.01 0.01 0.01 0 0
0.01 0.01 0 0 0.01 0 0.01 0.01
0 0 0 0 0 0.01 0.01 0.01
0.01 0 0.01 0 0.01 0 0 0
0.2 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0 0 0 0 0.01 0 0.01 0
0 0 0.01 0.01 0 0.01 0.01 0.01
0.01 0.01 0 0 0 0 0 0
0.01 0 0 0 0 0.01 0 0
0.6 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8
0 0 0 0 0 0 0 0
0.01 0 0 0 0.01 0.01 0 0
0 0.01 0.01 0 0.01 0.01 0.01 0.01
0.01 0.01 0 0.01 0.01 0.01 0 0
0.01 0 0 0.01 0 0.01 0 0
0.06 mg / 200 gm rat
Graded-Dose Response Curve SECTION A Paracetamol
Foot Volume
Preparation:500 mg tablet Doses: 0.5 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0.01 0 0 80
0.01 0.01 0.01 0 0.01 0.01 0 0.01 0.01 0 173.3
0 0.01 0.01 0 0 0 0.01 0 0 0 126.7
0 0.01 0.01 0.01 0.01 0.01 0.01 0 0.01 0 180.0
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0.01 0 0 0 0 0 0 0 0 100
0.01 0.01 0.01 0 0.01 0 0.01 0.01 0.01 0 192.9
0 0 0.01 0.01 0.01 0.01 0 0.01 0 0 142.9
0.01 0 0.01 0 0.01 0.01 0 0.01 0.01 0 192.9
5 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0.01 0 0 0.01 0 0 0 188.9
0 0 0.01 0.01 0 0 0.01 0 0 0 255.6
0 0 0.01 0 0 0 0.01 0 0 0 255.6
0 0.01 0.01 0.01 0.01 0 0 0.01 0 0 322.2
16 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 110.0
0 0.01 0 0.01 0 0.01 0.01 0.01 0 0 280.0
0.01 0 0.01 0 0.01 0.01 0.01 0 0.01 0 320.0
0.01 0.01 0.01 0.01 0.01 0 0 0.01 0 0 290.0
1.6 mg / 200 gm rat
Graded-Dose Response Curve SECTION B Aspirin
Foot Volume
Preparation:200 mg tablet Doses: 0.5 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0.01 0 0 0 38.5
0 0.01 0 0 0 0.01 0 0 0 0 84.6
0 0 0 0 0 0 0 0 0 0 -38.5
0 0 0 0 0 0 0 0 0 0 23.1
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0.01 0.01 0 0.01 0 0 29.2
0 0 0 0 0 0 0 0 0 0 -20.8
0 0 0 0 0 0 0 0 0 0 -20.8
0 0 0 0 0 0 0 0 0 0 -33.3
5 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0.01 0.01 0 0
0 0 0 0 0 0 0.01 0.01 0 0 7.4
0 0 0 0 0.01 0 0.01 0 0 0 -14.8
0 0 0 0 0.01 0 0 0 0 0 -22.2
0 0 0 0 0 0 0 0 0 0 -48.1
16 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0 0 0 0 0 0 -33.3
0 0.01 0 0 0 0 0 0 0 0 13.3
0 0 0 0 0 0 0 0 0 0 6.7
0 0 0 0 0 0.01 0 0.01 0 0 33.3
1.6 mg / 200 gm rat
Graded-Dose Response Curve SECTION C Celecoxib
Foot Volume
Preparation:200 mg tablet Doses: 0.3 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0
0.01
0
0
0.01 0 0.01 0 0 0.01 0 0 115.4
0.01 0 0 0 0 0.01 0 0 130.8
0 0 0.01 0 0 0 0 0 76.9
0.01 0 0.01 0 0 0 0 0 84.6
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0.01 0 0 0.01 0 0 0
0.01 0 0 0.01 0.01 0.01 0.01 0.01 0.01 0 55.2
0 0 0 0.01 0.01 0 0 0 0 0 3.4
0 0 0.01 0 0 0.01 0 0 0 0 -34.5
0.01 0 0 0.01 0 0.01 0 0 0 0 3.4
3 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0.01 0 0.01 0 0 0.01 0.01 0
0.01 0.01 0.01 0.01 0.01 0.01 0.01 0 0.01 0 51.6
0 0.01 0.01 0 0 0 0.01 0 0 0 16.1
0 0 0 0 0 0 0 0 0 0 -12.9
0 0 0 0 0 0 0 0 0 0 -41.9
10 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference
0 0 0.01 0.01 0.01 0.01 0.01 0.01 0.01
0.01 0.01 0.01 0.01 0 0.01 0.01 0.01 0.01 0
0.01 0.01 0.01 0.01 0.01 0 0.01 0 0 0
0.01 0 0 0 0 0 0 0 0 0
0 0 0 0.01 0 0 0 0.01 0 0
1 mg / 200 gm rat
% Difference
4.9
-7.3
-39.0
-31.7
Graded-Dose Response Curve SECTION D Prednisone
Foot Volume
Preparation:20 mg tablet Doses: 0.02 mg / 200 gm rat
Before
Carageenan
1 hr
2 hrs
3 hrs
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0.01 0 0.01 0 0 0 0.01 0 0 38.1
0.01 0.01 0.01 0.01 0.01 0.01 0.01 0 0 0 90.5
0 0 0 0 0 0 0 0 0 0 -4.8
0.01 0 0.01 0 0.01 0 0 0 0 0 23.8
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0.01 0 0.01 0.01 0.01 0 0 0 0 82.4
0.01 0.01 0 0 0.01 0 0.01 0.01 0 0 105.9
0 0 0 0 0 0.01 0.01 0.01 0 0 70.6
0.01 0 0.01 0 0.01 0 0 0 0 0 76.5
0.2 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean Difference % Difference
0 0 0 0 0 0 0 0 0
0 0 0 0 0.01 0 0.01 0 0 0 93.8
0 0 0.01 0.01 0 0.01 0.01 0.01 0 0 93.8
0.01 0.01 0 0 0 0 0 0 0 0 112.5
0.01 0 0 0 0 0.01 0 0 0 0 62.5
0.6 mg / 200 gm rat
Rat 1 Rat 2 Rat 3 Rat 4 Rat 5 Rat 6 Rat 7 Rat 8 Mean
0 0 0 0 0 0 0 0 0
0.01 0 0 0 0.01 0.01 0 0 0
0 0.01 0.01 0 0.01 0.01 0.01 0.01 0
0.01 0.01 0 0.01 0.01 0.01 0 0 0
0.01 0 0 0.01 0 0.01 0 0 0
0.06 mg / 200 gm rat
Difference % Difference
0 78.9
0 94.7
0 94.7
0 78.9
0
time Column H
Column G
Column F
Column E
Column D
foot volume
PARACETAMOL
0.01
0
0
0
0 Row 1 Row 2 Row 3 Row 5
foot volume 0 0 0
time
Column H
Column G
Column F
Column E
Column D
ASPIRIN
0
0 0
0 0 0
Row 6 Row 8 Row 9 Row 1
0
time
Column H
Column G
Column F
Column E
Column D
foot volume
CELECOXIB
0.01
0.01
0
0
0
0 Row 1 Row 1 Row 1 Row 1
PREDNISONE
time
Column H
Column G
Column F
Row 179 Row 192 Row 204 Row 216 Column E
Column D
foot volume
0.01 0 0 0 0 0 0 0 0 0 0
Column H
Row 14 Row 27 Row 39 Row 51
Column H
Row 69 Row 82 Row 94 Row 106
Column H
Row 124 Row 137 Row 149 Row 161
Column H
Row 179 Row 192 Row 204 Row 216
A
B
C
D
Dosage (mg) log (mg) After Carrageenan 0.5 -0.3 80.0 1.6 0.2 100.0 5 0.7 188.9 16 1.2 110.0 0.5 -0.3 38.5 1.6 0.2 29.2 5 0.7 7.4 16 1.2 -33.3 0.3 -0.52 115.4 1 0 55.2 3 0.48 51.6 10.0 1 4.9 0.02 -1.7 38.1 0.06 -1.22 82.4 0.2 -0.7 93.8 0.6 -0.22 78.9
% Response 1st hour
2nd hour 173.3 192.9 255.6 280.0 84.6 -20.8 -14.8 13.3 130.8 3.4 16.1 -7.3 90.5 105.9 93.8 94.7
126.7 142.9 255.6 320.0 -38.5 -20.8 -22.2 6.7 76.9 -34.5 -12.9 -39.0 -4.8 70.6 112.5 94.7
Paracetamol 325.0 300.0
250.0 225.0
Column D
200.0
Column E Column F
175.0
Column G
150.0 125.0 100.0 75.0 0
2
4
6
8
10
12
14
16
Dosage (mg)
Aspirin
% Response
C
% Response
275.0
90.0 80.0 70.0 60.0 50.0 40.0 30.0 20.0 10.0 0.0 -10.0
Column D Column E Column F Column G
% Response
60.0 50.0 40.0
Column D
30.0 20.0 10.0 0.0 -10.0 -20.0 -30.0 -40.0 -50.0
Column E Column F Column G
0
2
4
6
8
10
12
14
16
Dosage (mg)
Celecoxib 140.0 120.0
% Response
100.0 80.0 60.0
Column D
40.0
Column E Column F
20.0
Column G
0.0 -20.0 -40.0 -60.0 0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Dosage (mg)
Prednisone 120.0 110.0
% Response
100.0 90.0 80.0 70.0
Column D
60.0
Column E
50.0
Column F
40.0
Column G
30.0 20.0 10.0 0.0 -10.0 0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
0.5
0.55
0.6
% Re
50.0
Column F
40.0
Column G
30.0 20.0 10.0 0.0 -10.0 0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
0.5
0.55
0.6
Dosage (mg)
Paracetamol 325.0
% Response
300.0 275.0 250.0 225.0
Column D
200.0
Column E
175.0
Column F
150.0
Column G
125.0 100.0 75.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
log of dosage
% Response
Aspirin 90.0 80.0 70.0 60.0 50.0 40.0 30.0 20.0 10.0 0.0 -10.0 -20.0 -30.0 -40.0 -50.0 0.4 0.3 0.2 0.1
Column D Column E Column F Column G
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
log of dosage
1
1.1 1.2 1.3
-40.0 -50.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
log of dosage
Celecoxib 140.0 120.0
% Response
100.0 80.0 60.0
Column D
40.0
Column E Column F
20.0
Column G
0.0 -20.0 -40.0 -60.0 -0.75
-0.5
-0.25
0
0.25
0.5
0.75
1
log of dosage
Prednisone 120.0 110.0
% Response
100.0 90.0 80.0 70.0
Column D
60.0
Column E
50.0
Column F
40.0
Column G
30.0 20.0 10.0 0.0 -10.0 -1.8
-1.6
-1.4
-1.2
-1
-0.8
log of dosage
-0.6
-0.4
-0.2
3rd hour 180.0 192.9 322.2 290.0 23.1 -33.3 -48.1 33.3 84.6 3.4 -41.9 -31.7 23.8 76.5 62.5 78.9 B
After Carageenan injection 190.0 180.0
Column D Column E Column F Column G D
% Response
170.0 160.0 150.0 140.0 130.0 120.0 110.0 100.0 90.0 80.0 70.0 0.4 0.3 0.2 0.1
16
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Dosage (mg)
After 1 hour 280.0 270.0
Column D Column E Column F Column G
% Response
260.0 250.0 240.0 230.0 220.0 210.0 200.0
1
1.1 1.2 1.3
.55
Column F Column G
% Respons
Column E
240.0 230.0 220.0 210.0 200.0 190.0 180.0 170.0 0.4 0.3 0.2 0.1
16
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
1
1.1 1.2 1.3
1
1.1 1.2 1.3
Dosage (mg)
After 2 hours 320.0
Column D Column E Column F Column G
% Response
300.0 280.0 260.0 240.0 220.0 200.0 180.0 160.0 140.0 120.0 0.4 0.3 0.2 0.1
10.0
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Dosage (mg)
After 3 hours
Column D Column E Column F Column G
0.6
% Response
0
Column D
250.0
330.0 320.0 310.0 300.0 290.0 280.0 270.0 260.0 250.0 240.0 230.0 220.0 210.0 200.0 190.0 180.0 170.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Column G
.55
0.6
% Re
Column F
240.0 230.0 220.0 210.0 200.0 190.0 180.0 170.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Dosage (mg)
Column D Column E Column F Column G
1.2 1.3
Column D Column E Column F Column G
1.2 1.3
1
1.1 1.2 1.3
1.2 1.3
Column D Column E Column F Column G
1
Column D Column E Column F Column G
-0.2
jection
Column D
0.9
1
1.1 1.2 1.3
Column E
Column E
0.9
1
1.1 1.2 1.3
Column F
0.9
1
1.1 1.2 1.3
Column G
0.9
1
1.1 1.2 1.3
Column G
0.9
1
1.1 1.2 1.3
A
B
C
% Inhibition of Inflammation 1st hour 2nd hour -173.3 -126.7 -192.9 -142.9 -255.6 -255.6 -280.0 -320.0 -84.6 38.5 20.8 20.8 14.8 22.2 -13.3 -6.7 -130.8 -76.9 -3.4 34.5 -16.1 12.9 7.3 39.0 -90.5 4.8 -105.9 -70.6 -93.8 -112.5 -94.7 -94.7
After Carageenan injection -80.0 -90.0 -100.0 -110.0 -120.0 -130.0 -140.0
Column D
-150.0 -160.0 -170.0 -180.0 -190.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
log of dosage
ibition of inflammation
C
% inhibition of inflammation
D
Dosage (mg) log (mg) After Carrageenan 0.5 -0.3 -80.0 1.6 0.2 -100.0 5 0.7 -188.9 16 1.2 -110.0 0.5 -0.3 -38.5 1.6 0.2 -29.2 5 0.7 -7.4 16 1.2 33.3 0.3 -0.52 -115.4 1 0 -55.2 3 0.48 -51.6 10.0 1 -4.9 0.02 -1.7 -38.1 0.06 -1.22 -82.4 0.2 -0.7 -93.8 0.6 -0.22 -78.9
After 1 hour -170.0 -180.0 -190.0 -200.0 -210.0 -220.0 -230.0 -240.0 -250.0 -260.0
Column E
% inhibition of inf
-210.0 -220.0 -230.0
Column E
-240.0 -250.0 -260.0 -270.0 -280.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
% inhibition of inflammation
log of dosage
After 2 hours -120.0 -140.0 -160.0 -180.0 -200.0 -220.0
Column F
-240.0 -260.0 -280.0 -300.0 -320.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
1
1.1 1.2 1.3
% inhibition of inflammation
log of dosage
After 3 hours -170.0 -180.0 -190.0 -200.0 -210.0 -220.0 -230.0 -240.0 -250.0 -260.0 -270.0 -280.0 -290.0 -300.0 -310.0 -320.0 -330.0 0.4 0.3 0.2 0.1
Column G
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
log of dosage
1
1.1 1.2 1.3
% inhibi
-300.0 -310.0 -320.0 -330.0 0.4 0.3 0.2 0.1
0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
log of dosage
1
1.1 1.2 1.3
on 3rd hour -180.0 -192.9 -322.2 -290.0 -23.1 33.3 48.1 -33.3 -84.6 -3.4 41.9 31.7 -23.8 -76.5 -62.5 -78.9
80.0 100.0 188.9 110.0 38.5 29.2 7.4 -33.3 115.4 55.2 51.6 4.9 38.1 82.4 93.8 78.9 B
n
Column D
D
1 1.2 1.3
Column E
173.3 192.9 255.6 280.0 84.6 -20.8 -14.8 13.3 130.8 3.4 16.1 -7.3 90.5 105.9 93.8 94.7
126.7 142.9 255.6 320.0 -38.5 -20.8 -22.2 6.7 76.9 -34.5 -12.9 -39.0 -4.8 70.6 112.5 94.7
180.0 192.9 322.2 290.0 23.1 -33.3 -48.1 33.3 84.6 3.4 -41.9 -31.7 23.8 76.5 62.5 78.9
Column E
.1 1.2 1.3
Column F
.1 1.2 1.3
1 1.2 1.3
Column G
1 1.2 1.3
