Bio 1 • Recombinant DNA Technology and The Human Genome Project SDJacinto Institute of Biology UP Diliman
Bibliographic Entry
Result (w/surrounding text)
Standardized Result
Mitchel, Campbell Reece. Biology Concept and Connections. California, 1997.
"At actual size, a human cell's DNA totals about 3 meters in length."
3.0 m
McGraw Hill Encyclopedia of Science and Technology. New York: McGraw Hill, 1997.
"If stretched out, would form very thin thread, about 6 feet (2 meters) long."
2.0 m
Matthews, Harry R. DNA Structure Prerequisite Information. 1997.
"The length is (length of 1 bp)(number of bp per cell) which is (0.34 nm)(6 × 109)"
2.0 m
Leltninger, Albert L. Biochemistry. New York: Worth, 1975.
"Chromosome 13 contains a DNA molecule about 3.2 cm long."
1.5 m
"Cell." The World Book Encyclopedia. Chicago: Field Enterprises, 1996.
"On the average, a single human chromosome consists of DNA molecule that is about 2 inches long."
2.3 m
http://hypertextbook.com/facts/1998/StevenChen.shtml
The DNA unraveled Nucleosome
Bases
Double helix
Chromosome http://www.molecularlab.it/images/varie/dna_structure.jpeg
C
C
www.cse.ucsc.edu/.../ biology_starter/DNA.html
http://cellbio.utmb.edu/cellbio/DNA-RNA.jpg
http://www.geneticengineering.org/chemis
http://www.bioteach.ubc.ca/MolecularBiology/AMonksFlourishingGarden/translatio
Recombinant DNA technology • DNA from one source is cut and combined with DNA from another source. • Donated DNA representing a gene is cut with restriction endonucleases (RE) • RE cuts at very specific sequence of bases. • Host DNA is also cut. • Another enzyme (ligase) glues the two cut pieces together. • This engineered DNA is then inserted into an cell such as bacteria or yeast.
http://ghs.gresham.k12.or.us/science/ps/sci/ibbio/biotech/pics/recombinant.gif
Significance • Has applications in improvement of livestock and agricultural produce and therapy of certain diseases, – Some plants made resistant to pests, diseases and drought due to Recombinant DNA technology – Human insulin is now expressed from recombinant genes expressed by bacterial vector.
Recombinant DNA tech applied- examples • Genetically modified organisms (GMOs)-applications of recombinant DNA technology – Transgenic organisms-officially defined as “one whose genome has been modified by externally applied new DNA; a term applied to metazoans”
soybeans
BT corn
http://images.google.com.ph/imgres?imgurl=http://www.scq.ubc.ca/quarterly023/GMcrop.gif&imgrefurl=http://www.scq.ubc.ca/quarterly023/0203hall.html&usg=__zZbigA2ho16mIJg1KK53P-w6hE=&h=281&w=360&sz=24&hl=en&start=1&tbnid=iiU_UUuqayuU9M:&tbnh=94&tbnw=121&prev=/images%3Fq%3DB T%2Bcorn%26gbv%3D2%26hl%3Den%26sa%3DG
Other interesting transgenic organisms – The case of Herman -the transgenic bull containing foreign gene lactoferrin- iron containing protein. • Herman has sired many calves all with lactoferrin gene • Successful breeding of Herman and progeny, a new source of nutritious milk may become available; has far-reaching effects for children in developing nations.
http://www.ifgene.org/herman.jpg
GENETIC MODIFICATION HAS NO PLACE IN THE WORLD OF FOOD www.kids.organics.org/ Organic/5.htm
http://images.google.com.ph/imgres?imgurl=http://www.nextnature.net/research/wpcontent/uploads/2007/07/029_crop.jpg&imgrefurl=http://www.nextnature.net/%3Fp%3D1283&usg=__ZCgRB7c_BJax2QHCGsC6oT1QqMY=& h=356&w=530&sz=98&hl=en&start=8&tbnid=JsjfF34ZKHquvM:&tbnh=89&tbnw=132&prev=/images%3Fq%3DGreen%2BPeace%2Band%2B GMOs%26gbv%3D2%26hl%3Den%26sa%3DG
http://blogs.venturacountystar.com/greenberg/archives/qqxsgGMOArk.jpg
Recombinant DNA technology applied
peer.tamu.edu/.../ module_4/whyitmatters.htm
What’s it all about? • Doctors assess DNA samples to detect presence of heritable diseases • With gene therapy they can replace defective or absent genes to direct normal function • The case of defective p53 genes and carcinogenesis
www.york.ac.uk/depts/ biol/units/cru/gendeliv.htm
http://labspace.open.ac.uk/file.php/2622/SK195_5_002i.jpg
http://ghr.nlm.nih.gov/handbook/illustrations/therapyvector.jpg
The Human genome project • started in 1990 finished in April 2003 • objective is to know the DNA sequence of the whole human genome ( the totality of DNA in any human cell) • was supported by the US Department of Energy and National Institutes of Health. During the early years of the HGP, the Wellcome Trust (U.K.) became a major partner; additional contributions came from Japan, France, Germany, China, and others.. • Celera-private organization based in Maryland also conducted similar project; in effect a competitor http://www.ornl.gov/sci/techresources/Human_Genome/home.shtml
Project goals • identify all the approximately 20,000-25,000 genes in human DNA, • determine the sequences of the 3 billion chemical base pairs that make up human DNA, • store this information in databases, • improve tools for data analysis, • transfer related technologies to the private sector, and • address the ethical, legal, and social issues (ELSI) that may arise from the project. http://www.ornl.gov/sci/techresources/Human_Genome/home.shtml
Basic Facts revealed from human genome project • The human genome contains 3.2 billion chemical nucleotide bases (A,G,C,T) • Average gene contains 3000 bases but varies greatly; largest known human gene is dystrophin with 2.4 million bases
We know what some of those genes do! • Tentative assignments for some of the gene functions have been made for half of the genes of the identified human DNA sequence • Functions of the other half still unknown; the exact functions of the “known” genes not even clear
We have the most genes, but… • In functional categories, humans have more genes than all other organisms whose genes have been sequenced. • Plants have more metabolic genes! than any other animal whose genes have been sequenced.
Significance of Human genome Project • Can be of help in treatment of diseases; (for instance one common goal of many laboratories is to utilize the information from the human genome project to unravel the molecular mechanism of Down’s syndrome; so far no success )
www.goma.demon.co.uk/ genetics/geneprinter.html
What’s left to be done? • Just about everything! The sequence of the human genome has been made available for everybody’s use and interpretation.
http://nhs.needham.k12.ma.us/cur/baker_00/2001_p2/baker_dc_ad_p.2/polit.%20cart2..gif
Pharmacogenomics • Study of how variations in the human genome affect the response to medications • Environment, diet, age, lifestyle, and state of health all can influence a person's response to medicines, but understanding an individual's genetic makeup is thought to be the key to creating personalized drugs with greater efficacy and safety. • May permit drugs to be tailor-made for individuals and adapted to each person's own genetic makeup.
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The cases of BRCA1 and 2 and PTPN1 • BRCA1 and 2- tumor suppressors that if mutated can cause breast cancer
Genes involved in stroke • H63D genetic defect in the HFE gene • one of the most common inheritable genetic defects, especially in Europe, – where it's estimated one out of four people carry the defective gene in northern Europe. – In southern Europe, even more people may carry this genetic defect. – leads to iron overload in the body, eventually causing organ dysfunction, diabetes, and liver cirrhosis, and now stroke.
Nutrigenomics • The study of how different foods may interact with specific genes to increase the risk of common chronic diseases such as type 2 diabetes, obesity, heart disease, stroke and certain cancers. • The premise underlying nutrigenomics is that the influence of diet on health depends on an individual's genetic makeup.
www.globecartoon.com/ neweconomy/13.html
Thank you for your attention..
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