Working Slides For Ampalaya

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EFFECT OF M. CHARANTIA (BITTER GOURD) CRUDE FRUIT EXTRACT AGAINST ISONIAZID, RIFAMPIN AND PYRAZINAMIDE TOXICITY IN MALE ALBINO RATS

January 03, 2008 Rm. 204 1

SUBSECT A3 Atanga, Pascal Atazan, Judy Carissa Atienza, Bryan Jason Atutubo, Cosette Esmeralda Austria, Angeli Alexie Avanceña, Fatima Marie Balan, Ralph Adriel Balaoing, Gracia Domini Bambico, Danilyne Joy Banzon, Crisel Margaret Barcarse, Fraian Barcenas, Reigna Mae Barrera, Diana Jane Barsaga, Neil Christian Bascos, Bryant Basilio, Francis Angelo Batac, Jireh Ann Bautista, Alyssa Bautista, Rochelle Ann

2

Introduction 3

Introduction TB

Anti-TB Ampalaya

4

Significance of Study 5

Significance of Study • Anti TB drugs are hepatotoxic. • Ampalaya juice may offer some form of liver protection in PTB patients under this form of therapy • To some extent, by an unknown mechanism, ampalaya may also induce liver damage. This study might be able to explore one form of drug-herb interaction which may result to an additive or synergistic effect to cause hepatotoxicity.

Objective of Study 7

Main objectives • To determine the effect of Ampalaya in Anti-TB drug induced hepatotoxicity in Male Albino rats

Specific Objective • To observed hepatotoxicity induced my Anti-TB drugs • To observe hepatoprotective effect of ampalaya • To determine possible drug-herb interraction

METHODOLOGY

Methodology

Acclimatized for 1 week

For 2 weeks Rats sacrificed via Cervical Dislocation Liver dissected and undergone histopath

Extraction of Ampalaya

Formulation of Anti-TB Drug

Drug Intervention

Results

Normal Rat’s hepatocytes(LPO) slide A2

Normal rat’s hepatocytes(HPO) slide A9

Rat’s hepatocytes showing mild congestion with hemorrhage (LPO) - slide B2

Rat’s hepatocytes showing moderate congestion with hemorrhage (LPO)slide B1

Rat’s hepatocytes showing moderate congestion with hemorrhage (HPO)slide B1

Rat’s hepatocytes showing individual cell necrosis(HPO) - slide C3

Rat’s hepatocytes showing submassive necrosis(LPO) slide C6

Rat’s hepatocytes showing submassive necrosis(HPO) slide C6

hepatocytes showing intracytoplasmic vacuoles (HPO) - slide A8

Rat’s hepatocytes showing intracytoplasmic vacuoles (HPO) slide

Statistical Analysis 29

Statistical Analysis

Statistical Analysis

Statistical Analysis

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