EFFECT OF M. CHARANTIA (BITTER GOURD) CRUDE FRUIT EXTRACT AGAINST ISONIAZID, RIFAMPIN AND PYRAZINAMIDE TOXICITY IN MALE ALBINO RATS
January 03, 2008 Rm. 204 1
SUBSECT A3 Atanga, Pascal Atazan, Judy Carissa Atienza, Bryan Jason Atutubo, Cosette Esmeralda Austria, Angeli Alexie Avanceña, Fatima Marie Balan, Ralph Adriel Balaoing, Gracia Domini Bambico, Danilyne Joy Banzon, Crisel Margaret Barcarse, Fraian Barcenas, Reigna Mae Barrera, Diana Jane Barsaga, Neil Christian Bascos, Bryant Basilio, Francis Angelo Batac, Jireh Ann Bautista, Alyssa Bautista, Rochelle Ann
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Introduction 3
Introduction TB
Anti-TB Ampalaya
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Significance of Study 5
Significance of Study • Anti TB drugs are hepatotoxic. • Ampalaya juice may offer some form of liver protection in PTB patients under this form of therapy • To some extent, by an unknown mechanism, ampalaya may also induce liver damage. This study might be able to explore one form of drug-herb interaction which may result to an additive or synergistic effect to cause hepatotoxicity.
Objective of Study 7
Main objectives • To determine the effect of Ampalaya in Anti-TB drug induced hepatotoxicity in Male Albino rats
Specific Objective • To observed hepatotoxicity induced my Anti-TB drugs • To observe hepatoprotective effect of ampalaya • To determine possible drug-herb interraction
METHODOLOGY
Methodology
Acclimatized for 1 week
For 2 weeks Rats sacrificed via Cervical Dislocation Liver dissected and undergone histopath
Extraction of Ampalaya
Formulation of Anti-TB Drug
Drug Intervention
Results
Normal Rat’s hepatocytes(LPO) slide A2
Normal rat’s hepatocytes(HPO) slide A9
Rat’s hepatocytes showing mild congestion with hemorrhage (LPO) - slide B2
Rat’s hepatocytes showing moderate congestion with hemorrhage (LPO)slide B1
Rat’s hepatocytes showing moderate congestion with hemorrhage (HPO)slide B1
Rat’s hepatocytes showing individual cell necrosis(HPO) - slide C3
Rat’s hepatocytes showing submassive necrosis(LPO) slide C6
Rat’s hepatocytes showing submassive necrosis(HPO) slide C6
hepatocytes showing intracytoplasmic vacuoles (HPO) - slide A8
Rat’s hepatocytes showing intracytoplasmic vacuoles (HPO) slide
Statistical Analysis 29
Statistical Analysis
Statistical Analysis
Statistical Analysis