Virtual Cell Culture

in silico

Virtual tissue culture Grow, manipulate, and analyze cells

in vivo

E

ndogenics offers services for modeling and simulation of the development, dynamic organization, and function of living tissue. Cells interact with and adapt to their microenvironment through regulatory processes, metabolism, and controlled gene expression. Virtual tissues are suitable for studying growth, signaling, differentiation, tumorigenesis, and other processes.

in vitro

Advancing research by integrating computer modeling and wet-bench approaches simple or complex dynamic, multi-cellular systems analysis of data from individual cells or populations hypothesis testing and in vivo prediction Rapid and convenient pilot experiments build, test, & refine cell regulatory networks gene alterations (knockouts, knockdowns, ...) network perturbations (RNAi, dose-response, ...) micromanipulations (injections, cell ablations, ...)

Complementing wet-bench biology

alter culture conditions (media, substrates, ...)

cell count

30

lineage marker .30

compactness

.80

A dynamic cell mass experimentally manipulated to study homeostasis and shape stability (L-R, metabolic and gene regulatory network; graph of cell mass developing from a single cell; growth advantage of a mutated cell (orange) causes the cell mass to reorganize). 60

a Courtesy of C. Leung (HMS)

b

[Matrix contact resource]

c

average apical basal

Cell a

Cell b

Cell c

Analysis of mammary acini (L-R, thin optical section of human mammary acinus in Matrigel™; cross-section of virtual mammary acinus; inset: three polarized cells; resource distribution in individual polarized cells).

Endogenics TM

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