Tissue Plant Culture By Mr Solomon Dayanithi

We Thanks to Dr Dinesh Goyal, STEP, Patiala

We Thanks to Mr K Chetty, Agri business Incubator, A.P.

D. Solomon Dayanithi NGO, CEO (BIOH) Hosur

Introduction •  What is Plant Tissue Culture ? o Practice used to propagate plants under sterile conditions, often clones of a plant. o Tissue culture is a technology used for mass propagation of elite and high yielding mother plant which gives disease free, uniform, genetically pure and highly potential plants in large scale in shortest period of time

Tissue Culture Products •  Fruit Crops: Banana, G-9, (Musa special) Robusta, Red Banana, Hill Banana, Elakki, Nendren, Malbhog (From Assam), Williams, Fig,Strawberry. •  Ornamental Plants: Gerbera, Anthuriums, Orchids, Syngonium. •  Spice Plants: Ginger, Turmeric, Vanilla, Planifolia •  Medicinal Plants: Aloebarbadensis, Stevia, Alpinia •  Commercial Crops: Sugarcane, Potato, Neem, Golden Bamboo •  Organic Manures

Advantages & Benefits 1.  Profitable oriental Business 2.  Better establishment in the field due to accelerated growth and well development root system for better adsorption. 3.  Best quality plants, uniform healthy plants. 4.  High yield in short term. 5.  Identical and true to the type of mother plant 6.  Availability of plants through out the year. 7.  Enervating and high returns 8.  Pest and disease free plants. 9.  Early maturity of crop.

Bio Technology •  Provides a potential to produce new improved safer and less expensive products and processes. •  Immediate application would be tissue culture for the fast multiplication of superior clones of plants.

Basic facilities & Infrastructure

1.  Area uninterrupted power supply. 2.  Hill environment(if possible) 3.

Fertile land with building 5.

4.

Water (Irrigation) facility

Low temperature climate

Lab Installation (For 1 million TC Plants) 1.  Laminar Air Flow 2.  Glassbead sterilizer with digital temperature controller 3.  Top loading balance with wind shield 4.  Gas auto clave vertical 5.  Tissue culture growth rack 6.  Electrical fitting 7.  Microprocessor based pH meter 8.  Magnetic stirrer 9.  SS Trolley 3 tiers with castor wheels 10.  Medium duty stirrer

Lab & Growth Level

•  Initiation

- Sappavation

•  Multiplication - Man Growth •  Rooting

- Separation of plants

•  Extra care

- Sales can be done in this stage also or can go for NET/POT growing section

•  Hardened plant- Grown Plant

Establishing A Unit/Company •  Scientific Lab : Farmer friendly site •  Laboratory

: S.S.I with minimum 2000 sq.ft area fully monitored

growth room to hold 1 million genuine TC plants. •  Green House spread over 1 acre and above

Investments •  Small size unit

: 10 Lakhs

•  Medium size unit : 20 Lakhs •  Large size unit

: Above 20 Lakhs to

50 Lakhs Subsidy : National Horticulture board 20% of the project cost

Tissue culture green house project report •  Yearly plant purchase cost : Rs 6521250 •  Expenses : Rs 1291306 •  Profit : Rs 3760444

THANK YOU