The Microscope
The Microscope Developed by Anton von Leuwenhoek Magnification – how many times the image appears larger than it actually is Resolution – how clear the image appears when viewed
The Microscope Compound Light Microscope • Series of glass lenses in a hollow tube • Shines beam of light through specimen • Specimens usually have to be stained with a chemical so they can be viewed • Can magnify from 10x to 1000x
The Microscope Scanning Electron Microscope • Shine beam of electrons onto surface of specimen • Produced 3D view of surface of specimen
The Microscope Transmission Electron Microscope • Shines beam of electrons through the specimen • View inside of specimen at high magnifications
The Microscope Parts and Functions Arm and Base are used to carry the microscope Base is what the scope sits on when placed on the table
The Microscope Parts and Functions Light source produces light that shines through the stage opening Under stage is a diaphragm that can be used to control the amount of light going through the specimen
The Microscope Parts and Functions Objective lenses will magnify specimen either 4 (scanning), 10 (low) or 40 (high) times Eyepiece will magnify the specimen 10 times
The Microscope Parts and Functions The total magnification of the specimen is the power on the eyepiece times the power on the objective EXAMPLE: Total scanning power is 10 (eyepiece) x 4 (scanning objective) = 40x
The Microscope Parts and Functions Coarse adjustment is used to get the specimen in focus on scanning and low powers (NOT HIGH!) Fine adjustment is used to get clear focus on any power
The Microscope Parts and Functions When a specimen is in focus on any power, turn the nosepiece that holds the objectives and fine focus to see the specimen DO NOT RAISE THE LENSES UP OR DOWN WHEN CHANGING POWERS