Squalene..pptx

RICH ALTERNATE SOURCES OF SQUALENE FROM SHEDDED LEAVES OF ARTOCARPUS LAKOOCHA, ROXB.- AN ENVIRONMENTAL CREDENTIAL, COST AND SAFETY. UNDER THE SUPERVISION OF

Dr. Suparna Mandal Biswas (ASSISTANT PROFESSOR) (Indina Statistical Institute,Kolkata) By MOUMITA MITRA M.Sc, 3rd semester

ROLL NO: - 31385216007 REG. NO: - 163131810004 OF 2016-2017

SQUALEN E ) is a structurally unique

Squalene (C30H50 triterpene compound that is one of the main components of skin surface lipids.[2]

It is an excellent oxygen carrier, which attributes deep-sea sharks to survive under a hostile environment.[2] Squalene also renders anti-cancer trait to sharks.[4][5] It is found in small quantities in some vegetable oils.[4] The commercial source of squalene is from the liver of deepsea sharks.[7]

Artocarpus lakoocha, Roxb. Artocarpus lakoocha is a valuable tree species native to India and used for fruit, furniture, timber and feed. The tree is 20 to 30 ft (6-9 m) tall with deciduous, large, leathery leaves, downy on the underside. The tree sheds huge amount of leaves towards the end of December till beginning of February.

Fig: Artocarpus lakoocha tree

A. lakoocha produces secondary metabolites and accumulates it in their shedded leaves, which has a negative allelopathy effect on the target organisms. [6]

OBJECTIVES OF THE STUDY  Explore the other plant sources of squalene.  Isolation, purification and molecular characterization of squalene from shedded leaves of Artocarpus lakoocha, Roxb..  Determining the antimicrobial activity of the purified squalene.

Collection of Artocarpus leaves

Fig: Shedded leaves of Artocarpus lakoocha

Isolation of allelochemicals from leaf dust of

Artocarpus lakoocha.

Fig: Sintered disc funnel with vacuum pump

Fig: Mechanical Stirrer

Fig: Rotary Vacuum Evaporator

Flow Diagram

Concentrated Methanolic Extract of Artocarpus lakoocha Extracted with Hexane

Residue

Fraction-1of ArtclHF

Extracted with Ethyl Acetate

Residue

Fraction-2 or ArtclEF

Extracted with Acetone

Residue

Fraction-3 or ArtclAF

Extracted with Methanol

Residue

Fraction-4 or ArtclMF

Purification Solvent Front Purified compound Solvent system= Pentane (100%)

 Rf= 0.83

Origin

Fig: Column chromatography

Fig: TLC plate showing single bright yellow spot in iodine vapour

Molecular Characterization of the Single Pure Compound Molecule + Sodium

Molecular Weight of Purified Compound= 410.38 g/ml.

Fig: Mass Spectra of ArtclHF

Molecular Characterization of the Single Pure Compound

C=C

C-H CH2

Fig: FTIR Spectra of ArtclHF

CH3 C-H

Molecular Characterization of the Single Pure Compound

C=C-H

8 CH3

10 CH2

Fig: 1 HNMR Spectra of ArtclHF

Molecular Characterization of the Single Pure Compound

15 Carbon

Fig:13 CNMR Spectra of ArtclHF

Molecular Characterization of the Single Pure Compound

6 CH

Fig: Dept-90 spectra of ArtclHF

Molecular Characterization of the Single Pure Compound

10 CH2

Fig: Dept-135 spectra of ArtclHF

8 CH3

Molecular structure of ArtclHF

Fig: Squalene (C30H50); Mol. wt.410.38

Microscopic View of Bacterial species

Fig: Sarcina lutea

Fig: Streptococcus aureus

Fig: Escherichia coli

Antibacterial activity of Squalene

Sarcina lutea

Fig: Effects of ArtclHF at different concentration on Sarcina lutea,

Escherichia coli, and Streptococcus aureus .

Microscopic view of Fungal species

Fig: Aspergillus tamarii

Fig: Aspergillus niger

Fig: Aspergillus fumigatus

Antifungal activity of Squalene

Fig: Effects of ArtclHF at different concentration on Aspergillus fumigatus, Aspergillus tamarii and Aspergillus niger.

FINDINGS Shedded Artocarpus leaves would be the good plant sources for squalene. About 10-12% squalene have been isolated from shedded Artocarpus leaves. Potential uses of these squalene in cosmetic dermatology and drug additives. These plant sources would be environment friendly without hampering the ecological balance.

REFERENCES 1. 2.

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Psomiadou E, Tsimidou M. On the role of squalene in olive oil stability. J Agric Food Chem 1999;47:4025-32. Tilvis R, Kovanen PT, Miettinen TA. Metabolism of squalene in human fat cells. Demonstration of a two-pool system. J Biol Chem 1982;257:10300-5. Desai KN, Wei H, Lamartiniere CA. The preventive and therapeutic potential of the squalene-containing compound, Roidex, on tumor promotion and regression. Cancer Lett 1996;101:93-6. Murakoshi M, Nishino H, Tokuda H, Iwashima A, Okuzumi J, Kitano H, et al. Inhibition by squalene of the tumor-promoting activity of 12-Otetradecanoylphorbol-13-acetate in mouse-skin carcinogenesis. Int J Cancer 1992;52:950-2.4. Rao CV, Newmark HL, Reddy BS. Chemopreventive effect of squalene on colon cancer. Carcinogenesis 1998;19:287-90. Suparna Mandal Biswas, Nabanita Chakraborty, Shedded Artocarpus leaves – Good plant sources of natural squalene with potent antioxidant and antimicrobial activity –Alternative to marine animals,ResearchGate,2013 Cathpole OJ, Kamp JC, Grey JB. Extraction of squalene from shark liver oil in a packed column using supercritical carbon dioxide. Ind Eng Chem Res 1997;36:4318-24.