Slides For Histo Practical Exam

List of Slides for The Practical Exam 1). Cardiac Muscle (H & E)

Slide #5: Heart Wall; Cross Section Visible at the top of the photo are the Perkingy Fibers (large, “lumpy-looking” fibers), responsible for causing the contractions of the heart. Note: The nuclei of heart muscle cells is at their periphery as well H & E Staining (Low Magnification)

Slide #5: Heart Wall; Cross Section Visible at the top of the photo are the Perkingy Fibers (large, “lumpy-looking” fibers), responsible for causing the contractions of the heart.  Beneath the Perkingy Fibers are visible the Cardiac Muscle Cells, and at the periphery of these cells, stained a very definitive purple are the nuclei. H & E Staining (Medium Magnification)

2). Blood Smear (May-Grunwald-Giemsa Staining)

Blood Smear May-Grünwald-Giemsa Staining Neutrophil, a.k.a Polymorphonuclear Leukocytes (with 4 nuclei; depicting its “old” status). Pink Cytoplasm. Surrounding the Neutrophil, many Red Blood Cells (anucleated, of course) (High Magnification)

Blood Smear May-Grünwald-Giemsa Staining Neutrophil, a.k.a Polymorphonuclear Leukocytes (with 2 nuclei; depicting its “young” status, as compared to the previous picture). Pink Cytoplasm. (High Magnification)

TV Screen: Blood Smear

Eosinophil. The DEEPLY/INTENSELY RED STAINED granulocytes cover most of the cytoplasm and make this cell easily recognizable. The Nucleus also has only 2 lobes.

May-Grünwald-Giemsa Staining (High Magnification)

TV Screen: Blood Smear

Eosinophil. The DEEPLY/INTENSELY RED STAINED granulocytes cover most of the cytoplasm and make this cell easily recognizable. The Nucleus also has only 2 lobes May-Grünwald-Giemsa Staining (High Magnification)

TV Screen: Blood Smear

Basophil. Slight “pinky” border with dark blue granules COVERING THE ENTIRE CYTOPLASM. The cell is so internally granular that the nucleus is invisible. It is easy to differentiate between Basophils and other white blood cells due to its granularity. May-Grünwald-Giemsa Staining (High Magnification)

TV Screen: Blood Smear

Monocyte. Monocytes are MUCH LARGER THAN Neutrophils, and their kidney-bean shaped nuclei make them easily distinguishable May-Grünwald-Giemsa Staining (High Magnification)

TV Screen: Blood Smear Lymphocyte. Very small sized. Dark coloured cytoplasm with a small rim of pink-coloured cytoplasm. These cells are the B cell and the T cells. May-Grünwald-Giemsa Staining (High Magnification)

TV Screen: Blood Smear Platelets. Seen in this photograph in the left part of the TV screen. Responsible for blood-clotting. May-Grünwald-Giemsa Staining

(High Magnification)

TV Screen: Blood Smear Eosinophil (bottom & towards the left) and Neutrophil (atop the Eosinophil). Easily distinguishable due to the Eosinophils intense colour in the cytoplasm, and due to the Neutrophils multiple nuclei and pink cytoplasm. May-Grünwald-Giemsa Staining (High Magnification)

3). Lymph Node (H & E)

Slide #1: Lymph Node Visible in this photograph are the lymphatic outer sinuses (spaces within lymph nodes), as well as within the medulla, the medullary sinuses are visible The dense purple tissue is the cortex The majority of what is visible in this photo is the dark purple cortex  The resident cells (T Cells) are purple within the lymph nodules  The pink stained areas are merely connective tissue H & E Staining (Low Magnification)

Slide #1: Lymph Node In this photograph, prominently visible, are the cortex and lymphatic zones. H & E Staining (High Magnification)

4). Palatine Tonsil (H & E)

Slide #3: Palatine Tonsil Very visible in this photograph are the dark purple stained lymphocytes, and the “round-shaped” structures are the arteries with their very thick walls. At the lower left 1/3 of the photograph are visible the veins, which appear as elongated structures. H & E Staining (High Magnification)

5). Lingual Tonsil (H & E)

Slide #6:

Lingual Tonsil Visible in this photograph are: Mucous glands (left of image), and directly below them is visible a very large vein (orangy-red colour). As well, dark-purple stained region is the germinal center, within its periphery which are visible several arteries (to its left). Furthermore, the elongated-appearing structures which have the reddish staining are the venuoles. H & E Staining (Low Magnification)

6). Spleen (H & E)

Slide #2: Spleen The Capsule, trabeculae, trabecular veins and arteries, white pulp (PALS, lymphatic nodules), red pulp (splenic cords and sinuses), and pulp veins are visible. --The pulp veins are visible here as the most easily visible, reddish circular objects within the white pulp --The capsule is visible encircling the periphery --The sinusoids are MUCH smaller than the pulp veins, yet also have the same colour as the veins. In this picture, the sinusoids are dispersed throughout the image, with several concentrated near the capsule H & E Staining (Medium Magnification)

Slide #2: Spleen At the top of the image is visible the capsule, which appears as a band at the top of the photo. As well, prominently visible in this photo is the “pulp vein” in the center of the image, with its deep red colour. As well, several dark purple lymphatic nodules are visible.  NOTE: Lymphatic nodules ONLY appear during an immune response! H & E Staining (Medium Magnification)

Slide #2: Spleen Prominently visible in this photo is the “pulp vein” in the center of the image, with its deep red colour. As well, several dark purple lymphatic nodules are visible.  NOTE: Lymphatic nodules ONLY appear during an immune response! H & E Staining (Medium Magnification)

Slide #2: Spleen The most prominent feature of this image is the Trabeculae, which can be seen here branching and stemming from the right of the image. Within its center can be seen the trabecular artery. H & E Staining (Medium Magnification)

7). Thymus-Juvenile (H & E)

Slide #4: Thymus-Juvenile The light pink stained regions are the Medulla (Mature Thymocytes present); –ve Selection here The dark purple stained regions are the Cortex (Immature Thymocytes present); +ve Selection here  The lymphoid nodules are easily identifiable in the Cortex as several dark purple regions H & E Staining (Low Magnification)

TV Screen: Thymus-Juvenile

In this middle of this photo are visible a couple of easily identifiable Hassal’s Corpuscles (“Hassal Bodies”) ---Hassal Bodies, ONLY present in the Medulla  Surrounding the Hassal’s Corpuscles, the Cortex H & E Staining (Low Magnification)

Slide #4: Thymus-Juvenile In this picture is an easily identifiable Hassal’s Corpuscles (Hassal Body) ---Hassal Bodies, ONLY present in the Medulla ---Reticular cells can also be seen in the vicinity of the interior of the Hassal Body H & E Staining (High Magnification)

8). Thymus in Involution (H & E)

Slide #5: Thymus in involution Involution: A progressive decline or degeneration of normal physiological functioning occurring as a result of the aging process The white globular cells are adipose tissue The dark concentric regions within the cortex are hyalized cells  Note: The cortex is the dark purple stained regions (right of photo) containing the immature thymocytes  Note: The medullary region contains the mature thymocytes (center of photo), with the hyalized cells within it H & E Staining (Low Magnification)

Slide #5: Thymus in involution H & E Staining (High Magnification)

9). Thyroid Gland with Parathyroids (H & E)

Slide #4: Thyroid and Parathyroid Gland  At the centre of this image is a typical colloid cell, and surrounding it in a disk are the Thyroid Follicular Cells. Around the Thyroid Follicular Cells lies the Basal Lamina.

Although H & E DOESN’T stain for it and they are not readily visible in this image, Parafollicular cells (“C” cells; calcitonin producing) lie around the Thyroid Follicular Cells. H & E Staining (High Magnification)

Slide #4: Thyroid and Parathyroid Gland  Visible in this photo are the paraffin-embedded Adipose Tissue H & E Staining (High Magnification)

10). Adrenal Gland (H & E)

Slide #2: Adrenal Gland In this image are visible large PURPLE stained nuclei (i.e., similar staining pattern to Lymphocyte nuclei because the nucleus to cell size ratio is high in both these types of cells). H & E Staining (High Magnification)

Slide #2: Adrenal Gland Visible from the bottom left of the image to the top right of the image are the 3 regions of the gland: 1). Zona Glomerulosa 2). Zona Fasciculata

3). Zona Reticularis Note: There is also adrenal medulla as well. H & E Staining (Low Magnification)

Slide #2: Adrenal Gland From Right to Left in this image are: The Capsule and 1). Zona Glomerulosa and 2). Zona Fasciculata H & E Staining (Low Magnification)

11). Supraoptic and paraventricular nuclei of the hypothalamus (Gomori Staining)  NOTE: Supraoptic tested ONLY!

Slide #P: Supraoptic and paraventricular nuclei of the hypothalamus Visible in this photo: Supraoptic Nuclei Gomori Staining (High Magnification)

Slide #P: Supraoptic and paraventricular nuclei of the hypothalamus Visible in this photo: Paraventricular Nuclei (look “above and below” to the left of the raphe/slit in the middle of the image. The cluster of nuclei there are the paraventricular nuclei) Gomori Staining (High Magnification)

12). Hypophysis (Mallory Staining)

Slide #1: --Microscopic View Hypophysis Visible in this photo: 1). Acidophiles (Reddish-orange stained), 2). Basophiles (Blue stained), and 3). Connective tissue (stained blue) Visible as well: 4). “Chromophobes”: appear as empty “white” spaces (Chrom: colour phobes: “haters”) These cells secrete: Vasopressin and Oxytocin Mallory Staining (Low Magnification)

13). Nonhairy skin-longitudinal section (H & E)

Slide #2: Nonhairy Skin-longitudinal section Visible in this photo are several layers of the skin (from top left to bottom right): The 1). stratum corneum (the top layer), 2). stratum lucidum (red band), 3). stratum granulosum (dark purple band beneath the stratum lucidum), 4). stratum spinosum, and 5). stratum basale. The 6). connective tissue is beneath the stratum basale. The visible “grooves” between the stratum basale and the connective tissue provide for a more secure lock-and-key mechanism between the two layers. H & E Staining (Medium Magnification)

14). Hairy Skin-longitudinal section (H & E)

Slide #1: Hairy Skin-longitudinal section Visible in this photo are (from follicle out): The cortex, melanocytes (black specks at bulb of hair) with dermal papilla just inferior to them, the internal root sheath and the external root sheath, and the outside layer, the glossy membrane. H & E Staining (Medium Magnification)

Slide #1: TV Screen: Hairy Skin-longitudinal section  Prominently visible in the central part of this photo, a sebaceous gland. H & E Staining (High Magnification)

Slide #1: Hairy Skin-longitudinal section Visible in this photo are (from follicle out): The cortex, melanocytes (black specks at bulb of hair) with dermal papilla just inferior to them, the internal root sheath and the external root sheath, and the outside layer, the glossy membrane. H & E Staining (High Magnification)

Slide #1: Hairy Skin-longitudinal section  Visible in the centre of this photo as a coiled structure with black-“specks”, is the sweat gland H & E Staining

(High Magnification)

15). Hairy Skin-cross section (H & E)

Slide #2: Hairy Skin-cross section Seen in the centre of the photo are (from medulla outwards): The medulla, cortex, cuticle, internal root sheath, and external root sheath. H & E Staining (Low Magnification)

16). Lip (H & E)

Slide #1:

Lip Visible in this photo are: The Vermillion Zone (far right, subsurface layer pinkcoloured), sebaceous gland, muscle tissue, adipose tissue, and an artery. H & E Staining (Low Magnification)

17). Tongue-filiform and fungiform papillae (H & E)  Note: Fungiform NOT tested

Taste Buds

Foliate Papillae Note: The Tall structure of the Papillae

Fungiform Papillae Note: Mushroom Appearance

18). Tongue-filiform and foliate papillae and taste buds (H & E)

Filiform Papillae Note: These taste buds are VERY shallow and easy to recognize

19). Developing tooth (Mallory staining)

Slide #2: Developing Tooth Visible in this photo are (from far right to left): Pulp, Odontoblasts (secrete Dentin), Predentin, Dentin (with the thick, rich red canaliculae), Enamel, and to the far left, Ameloblasts=adamentoblasts (secrete enamel). H & E Staining (Low Magnification)

20). Adult tooth (Mallory Staining)

Slide #Z: Adult Tooth-Decalcified

Decalcification has dissolved the enamel of this tooth Mallory Staining (Low Magnification)

21). Parotid Gland (H & E)

Parotid Gland (Low Magnification)

Parotid Gland Note: Invaginations of the epithelium!!! Low Magnification

Slide #15: Parotid Gland The duct is prominently visible in the centre of this photo H & E Staining (High Magnification)

22). Sublingual gland (H & E)

Sublingual gland Note: Many mucous tubules; easy to distinguish between sublingual and submandibular

23). Submandibular Gland (H & E)

Slide #17: Submandibular Gland Ducts are visible at the centre of this photo Note: Presence of mucous tubules and prevalence of DARK serous elements H & E Staining (Low Magnification)

Submandibular Gland Ducts are visible at the centre of this photo

A great MIXTURE of MUCOUS TUBULES and STRIATED DUCTS NOTE: Appears MUCH DARKER than sublingual gland as well! H & E Staining

24). Esophagus (H & E)

Slide #6: Esophagus Visible at the the far right of this picture is the Stratified Squamous Epithelium H & E Staining (Low Magnification)

Slide #6: Esophagus Visible centrally located in this picture is the smooth muscles H & E Staining (Medium Magnification)

Slide #6: Esophagus Visible in the centre of this picture is an esophageal gland H & E Staining (Medium Magnification)

Slide #6: Esophagus Visible in this image are skeletal muscles H & E Staining (Medium Magnification)

25). Gastric fundus-staining for parietal cells (Kongo Red)

Slide #7: Gastric Fundus This slide has been stained for parietal cells Kongo Red Staining (Low Magnification)

Slide #7: Gastric Fundus  This slide has been stained for “Parietal Cells” o The orange cells located peripheral to the main cells are the “Parietal Cells”  The Parietal Cells are responsible for producing HCL  The cells which the Parietal cells are surrounding are the “Chief Cells”  The Chief Cells are responsible for producing Pepsinogen Kongo Red Staining (Low Magnification)

26). Gastric pylorus (H & E)

Slide #8: Gastric Pylorus Visible in this slide are (from far left to right): Muscularis Mucosae, the mucous secreting cells located adjacent to the Muscularis Mucosae, and cells of the Gastric Pits (dark purple cells which also produce mucous) Note: The nerves in the Gastric Plexus. These are nerves of the Auerbach’s Plexus H & E Staining (Low Magnification)

Slide #8: Gastric Pylorus Visible in this slide is a Nerve passing through the gastric pylorus Note: The nerves in the Gastric Plexus. These are nerves of the Auerbach’s H & E Staining (Medium Magnification)

27). Small intestine-duodenum (H & E)

Plexus

Slide #9: Small Intestine-duodenum Key Point for Duodenum: Has the LONGEST villi of the 3 regions of the small intestine!  Visible in this photo are (left to right): villi, the submucosal region with Brunner’s Glands and the Meissner Plexus, and to the far right, the muscle of the duodenum Although not visible, the duodenum also has Peyer’s Patches (Lymphoid nodules) H & E Staining (Low Magnification)

28). Small intestine-jejunum (H & E)

Slide #10:

Small Intestine-jejunum Key Point for Jejunum: Has MEDIUM SIZED villi of the three regions, but not as long as duodenum!  Visible in this photo are (left to right): the Meissner Plexus and connective tissue, BUT NO Brunner’s Glands present in Jejunum (contrast with Duodenum), and to the far right, the villi. Note: The layering of the duodenum, jejunum, and ileum are all the same, except that in the jejunum, there are NO Brunner’s Glands and NO Peyer’s Patches in jejunum, as opposed to the duodenum which DOES possess them. H & E Staining (Low Magnification)

29). Small intestine-ileum (H & E)

Small Intestine-ileum Key Point for Ilium: Has SHORTEST villi of the three regions!  Visible in this photo are (top to bottom): the SHORT VILLI, and Peyer’s Patches. Note: The layering of the duodenum, jejunum, and ileum are all the same. The differences are that the ileum has the SHORTEST VILLI and DOES HAVE PEYER’S PATCHES. H & E Staining

30). Large intestine-colon (H & E)

Slide #11: Large Intestine-colon H & E Staining (Low Magnification)

31). Large intestine-appendix (H & E)

Slide #12: Large Intestine-Appendix H & E Staining (High Magnification)

Large Intestine-Appendix Note: The circular structure of the specimen, as well as the LARGE collection of lymphoid nodules in the appendix H & E Staining (High Magnification)

32). Human liver (H & E)

Slide #1: Human Liver Centrally located in this photo is visible the Portal Space, with hepatocytes surrounding it

 At the top of the Portal Space is visible an artery (with its thick tunica media), and at the centre of the portal space is visible a massive vein.  At base of Portal Space is visible a Bile Duct (with blue stained central region).  Directly above the bile duct is an artery, and to the right of the artery and below the massive vein is a Lymph Vessel  Liver also has Ito Cells: Control Vasoconstriction. H & E Staining (High Magnification)

33). Swine liver (H & E)

Slide #2: Swine Liver Visible in this photo: Top of picture is a Lymph Vessel Bottom left of picture is the Portal Space The interlying space between the two has many hepatocytes in it. H & E Staining (Medium Magnification)

Slide #2: Swine Liver Visible in this picture is a Liver Sinusoid The purple stained regions are the hepatocytes, and within the liver sinusoids are visible erythrocytes which are red coloured. H & E Staining (Medium Magnification)

Slide #2: Swine Liver Visible in this picture is a Liver Sinusoid

The purple stained regions are the hepatocytes, and within the liver sinusoids are visible erythrocytes which are red coloured. H & E Staining (High Magnification)

34). Human liver (Mallory staining)

Slide #2A: Human Liver Visible in the centre of this photograph is the liver sinusoid Mallory Staining (Medium Magnification)

Slide #2A: Human Liver At the centre of this photo is the Central Vein. Surrounding the central vein are hepatocytes, which are all pointed towards the central vein concentrically! Mallory Staining (Medium Magnification)

Slide #2A: TV presentation Human Liver

 Visible are the Portal space (within which are erythrocytes, the artery at base of it)  To the right of portal space are visible Ito Cells (brown stained region) Mallory Staining (Medium Magnification)

35). Gall bladder (H & E)

Slide #10: Gall Bladder  Visible in this photograph are columnar epithelium (top layer), and beneath the columnar epithelium is a smooth muscle layer. Directly beneath this layer is the serosa. Note for the lab exam: The columnar epithelium folds in ALL DIRECTIONS. Also, there are NO GLANDS in the mucosal layer. H & E Staining (Medium Magnification)

36). Pancreas (H & E)

Slide #3: Pancreas  Centrally visible in this photograph is an “Islet of Langerhans” --Visible surrounding the Islet of Langerhans are the “Serous acinus,” which are exocrine cells H & E Staining (Medium Magnification)

37). Pancreas (Brachet’s staining)

Brachet’s staining stains for RNA, and visible under this staining in this slide are RIBOPROTEINS

38). Trachea-cross section (H & E)

Slide #1: Trachea Visible in this photo are the pseudostratified epithelium Note for the lab exam: LOTS OF HYALINE CARTILLAGE visible under the epithelium H & E and PAS Staining (High Magnification)

Slide #1: Trachea

Visible in this photo are: Veins (with dark “blotches” of endothelial cells surrounding them), connective tissue, as well as the pseudostratified epithelium. H & E and PAS Staining (Medium Magnification)

39). Lung Parenchyma (H & E)

Slide #2: Lung Parenchyma Visible in this photo are: Bronchi, Bronchioles, and Alveoli, which are part of the Conducting System of the Respiratory Tract. The terminal portion only has Single Alveoli, as opposed to the Respiratory Portion which has several Alveoli. As well, BRONCHIOLES contain NO CARTILLAGE, as opposed to BRONCHI which CONTAIN CARTILLAGE. H & E Staining (High Magnification)

Slide #2: Lung Parenchyma Visible in this photo are: Bronchi, Bronchioles, and Alveoli, which are part of the Conducting System of the Respiratory Tract. The terminal portion only has Single Alveoli, as opposed to the Respiratory Portion which has several Alveoli. As well, BRONCHIOLES contain NO CARTILLAGE, as opposed to BRONCHI which CONTAIN CARTILLAGE. H & E Staining (Low Magnification)

Slide #2: Lung Parenchyma Visible in this photo are: Vein and Alveoli.

H & E Staining (Low Magnification)

Slide #2: Lung Parenchyma Visible in this photo are: Bronchioles. H & E Staining (Low Magnification)

Slide #2: Lung Parenchyma

Visible in this photo are: Bronchioles. BRONCHIOLES, as visible here, contain NO CARTILLAGE, as opposed to BRONCHI which CONTAIN CARTILLAGE. H & E Staining (Low Magnification)

Slide #2: Lung Parenchyma Visible in this photo are: Bronchi, and clearly visible CHONDROCYTES (forming CARTILLAGE). As well, BRONCHIOLES contain NO CARTILLAGE, as opposed to BRONCHI which are clearly visible here and which DO CONTAIN CARTILLAGE. H & E Staining (Low Magnification)

40). Human kidney (H & E)

Slide #1: Human Kidney Centrally located in this photo is the Renal Corpuscle and visible is the Bowman’s Capsule;  Within the Bowman’s Capsule is the podocyte  To the left side of the podocyte is the Distal Tubule (w/only a fragment of the Afferent Arteriole visible)  To the right side of the podocyte is the Proximal Convoluted Tubule  Surrounding the podocyte is the Macula Densa H & E Staining (Medium Magnification)

Slide #1: Human Kidney Centrally located in this photo is visible the Bowman’s Capsule;  The pointer is pointing to the Distal Tubule within the Podocyte H & E Staining (Medium Magnification)

41). Kidney (injection specimen to demonstrate the vascular bed)

Slide #2: Human Kidney Injection specimen to demonstrate the vascular bed  This dye is a special type of liquid dye stain which forms into a gel  Two structures can be seen: 1). Very large vein dyed blue 2). A renal corpuscle within which is visible the Bowman’s capsule and Distal Tubule H & E Staining (Low Magnification)

Slide #2: Human Kidney Injection specimen to demonstrate the vascular bed Visible in the centre of this image is the Glomerulosa H & E Staining (Medium Magnification)

42). Ureter (H & E)

Slide #4: Human Ureter-transverse section Visible in this image is transitional epithelium

H & E Staining (Low Magnification)

Slide #4: Human Ureter-transverse section Visible in this image is transitional epithelium H & E Staining (Medium Magnification)

43). Urinary bladder (H & E)

Slide #6:

Human Urinary Bladder-cross section Visible in this image are the smooth muscles in the urinary bladder H & E Staining (Low Magnification)

Slide #6: Human Urinary Bladder-cross section Visible in this image are from (right to left): Transitional epithelium with Lamina Propria beneath it Note: Distinguishing characteristic is the smooth muscle layer & transitional epithelium KEY POINT: VACUOLES AROUND THE NUCLEUS!!! H & E Staining (Low Magnification)

Slide #6: Human Urinary Bladder-cross section Visible in this image are Nerve Bundles in the urinary bladder H & E Staining (Low Magnification)

44). Testis (H & E)

Slide #1: Human Testis H & E Staining (High Magnification)

Slide #1: Human Testis Visible in this picture are: Border of seminiferous tubules, Fibroblasts within the border of seminiferous tubules, Sertoli Cells (not round), and Gametogenic cells. H & E Staining (Low Magnification)

45). Epididymis and ductuli efferentes (H & E)

Slide #2: Human Epididymis

Visible in this picture is the Pseudostratified Epithelium of the Epididymis  These are the Ductuli Efferentes (No “cilia”), i.e., the head H & E Staining (High Magnification)

46). Spermatic cord with ductus deferens (H & E)

Slide #3: Human Epididymis Visible in this picture is the Ductus Deferens;  Visible prominently are the concentric circular smooth muscle layer (interior) and longitudinal smooth muscle layer (exterior) H & E Staining (Low Magnification)

47). Prostate gland (H & E)

Slide #3: Human Prostate Gland Visible in this picture is the fibromuscular stroma (pink) and the secretory elements (parenkyma) which are purplish colour; H & E Staining (Low Magnification)

48). Ovary (H & E)

Slide #5: Ovary Visible in this slide are (from top left of slide down to bottom right): Primordial follicles next to 1o follicles. This layer constitutes the germinative epithelium. The large follicle seen in the centre of the slide is a 2o Follicle, which is internally surrounded by purple stained by Granulosa Cells. Right beneath it are visible a couple of Atretic ova. H & E Staining (Medium Magnification)

Slide #5: Ovary Visible centrally in this slide is: A 2o follicle H & E Staining (Medium Magnification)

Slide #5: Ovary Visible in this slide is: A Graafian follicle Personal Note: The follicle is so massive that it takes up the entire field of view, and more, of the slide! H & E Staining (Medium Magnification)

Slide #5 (2nd one): Ovary Visible in this slide is: One massive Corpus Luteum is seen during pregnancy in women in their ovaries. H & E Staining (Medium Magnification)

49). Oviduct (H & E)

Slide #6: Oviduct Visible in this slide is (from top right to bottom left): Connective tissue and smooth muscle layer. The finger-like projections are simple ciliated columnar epithelium, and a covering layer of cilia. H & E Staining (Medium Magnification)

Slide #6: Oviduct Visible in this slide is: The finger-like projections are simple ciliated columnar epithelium, and a covering layer of cilia which is easily visible. H & E Staining (High Magnification)

50). Uterus (H & E)

Slide #6: Uterus Visible in this slide is (from top right to bottom left): The exterior perimetrium, the middle layer of myometrium (composed of a muscle layer of circular, spiral, and longitudinal fibers), and the internal endometrium layer (lined with mucosa, lamina propria, and a lining epithelium composed of simple columnar epithelium). H & E Staining (Low Magnification)

Uterus (mouse)

51). Spinal cord (Stained with osmium and eosin or H & E)

Slide #3 Spinal Cord Visible: gray matter, white matter, anterior roots, posterior roots, motor nerve cells  The dark stained regions are the grey matter (to the right of the field pf view), and the dark visible nuclei are the motor neurons in the ANTERIOR ROOTS Stained w/Osmic Acid and Eosin

Slide #3 Spinal Cord Visible in this picture is the Spinal Canal Stained w/Osmic Acid and Eosin

Slide #3 Spinal Cord Note: Posterior region. The neurons are much smaller and much less present Stained w/Osmic Acid and Eosin

52). Cerebellum (Silver impregnation)

Slide #2: Cerebellum Stained with Silver Impregnation

52). b. Cerebellum (H & E)

Slide #2: Cerebellum Visible is the Medullary Core (nerve fibers);  Visible in the darker, centre region is the Granular layer, with the Purkinje cell layer at the periphery of this layer. The lighter pink stained region is the White Matter.  Note: The Purkinje Cells are right at the border of the Granular layer

H & E Staining