Sample Data

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EXAMPLE GRAPH DATA 200 Data labels = % survival ± SEM

[parameter]/wet weight sample (ng/mg)

180

Treatment #1

160

Treatment #2

89.7% ± 1.1%

140

86.2% ± 2.0%

120

90.1% ± 0.7%

87.4% ± 1.5%

100 86.9% ± 1.7%

80

87.0% ± 1.3%

60 EXAMPLE PROTOCOL 1) Tissue was cut into approx. 1.0 cm x 1.0 cm pieces, and wet weight was measured for each sample 2) A single donor was used for all treatments performed in duplicate 3) Cell viability was measured with the LIVE/DEAD assay immediately after 8 hour digestion

40

20

86.8% ± 2.3%

0 0

500

1000

1500 [Enzyme] (Units)

2000

2500

3000

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