EXAMPLE GRAPH DATA 200 Data labels = % survival ± SEM
[parameter]/wet weight sample (ng/mg)
180
Treatment #1
160
Treatment #2
89.7% ± 1.1%
140
86.2% ± 2.0%
120
90.1% ± 0.7%
87.4% ± 1.5%
100 86.9% ± 1.7%
80
87.0% ± 1.3%
60 EXAMPLE PROTOCOL 1) Tissue was cut into approx. 1.0 cm x 1.0 cm pieces, and wet weight was measured for each sample 2) A single donor was used for all treatments performed in duplicate 3) Cell viability was measured with the LIVE/DEAD assay immediately after 8 hour digestion
40
20
86.8% ± 2.3%
0 0
500
1000
1500 [Enzyme] (Units)
2000
2500
3000