Rr322306-protein-engineering

Set No. 1

Code No: RR322306

III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 PROTEIN ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Write short notes on: (a) Edman degradation (b) Identifying Amino terminal and Carboxy terminal residues.

[8+8]

2. Justify the statement that “tertiary structure is more organized than secondary structure”. [16] 3. Define post-translational Modification? Explain Post-translational modification by alteration of chain termini? [16] 4. Describe the zinc finger and their function.

[16]

5. Name the membrane protein which is present in Halobacterium salinarium ? Explain about mechanisms of light driven proton pump in Bacteriorhodopsin? [16] 6. Discuss the structure of Insulin and describe its functions.

[16]

7. Write about Pattern databases for storing information about protein families and motifs? [16] 8. (a) What do you mean by protein design? (b) What are the goals of protein design? ⋆⋆⋆⋆⋆

1 of 1

[8+8]

Set No. 2

Code No: RR322306

III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 PROTEIN ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Describe the characterization of protein structure?

[16]

2. Describe the role of hydrophobic forces in tertiary structure.

[16]

3. (a) What are chaperons? (b) Describe types of chaperons and their role in folding of protein. 4. Describe the zinc finger and their function.

[8+8] [16]

5. Describe the properties of three classes of Membrane proteins, how they differ from one other and how they vary among themselves? [16] 6. Describe the sequence of events following the absorption of photon of light by photosynthetic reaction center pigment of PS-II. Describe the comparable events in PS-I. How are two photosystems linked to one another? [16] 7. Write a note on Curated family databases for Protein?

[16]

8. (a) What do you mean by protein design? (b) What are the goals of protein design? ⋆⋆⋆⋆⋆

1 of 1

[8+8]

Set No. 3

Code No: RR322306

III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 PROTEIN ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Explain Secondary structure prediction from protein sequences?

[16]

2. Describe the role of hydrogen bonds in tertiary structure of protein.

[16]

3. Describe Posttranslational modifications by proteolytic cleavage.

[16]

4. Explain in detail about the different Transcription factors that contains Zinc as an essential element of their DNA binding domains? [16] 5. (a) What are proteases. (b) Describe different classes of proteases with examples. (c) Explain the action of serine proteases in details.

[5+6+5]

6. Explain the process of recognition of foreign molecule by Immunoglobulins.

[16]

7. Write a note on engineering complex human proteins by large scale mammalian cell culture? [16] 8. Explain the importance of protein designing in the field of Drug designing. ⋆⋆⋆⋆⋆

1 of 1

[16]

Set No. 4

Code No: RR322306

III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 PROTEIN ENGINEERING (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Discuss about General properties of Proteins Secondary structure?

[16]

2. Write short notes on: (a) Myoglobin (b) Globular proteins

[8+8]

3. Justify statement “Final structure of protein is not determined by Gene structure but by the primary structure of protein”. [16] 4. Write short notes on: (a) Zinc fingers (b) Leucine zippers

[8+8]

5. Name the membrane protein which is present in Halobacterium salinarium ? Explain about mechanisms of light driven proton pump in Bacteriorhodopsin? [16] 6. (a) What are Immunoglobulins. (b) Describe the structure of Immunoglobulin. (c) Describe the various classes of Immunoglobulins and their properties. [5+5+6] 7. Write short notes on: (a) PROSITE (b) BLOCKS

[8+8]

8. (a) What do you mean by protein design? (b) What are the goals of protein design? ⋆⋆⋆⋆⋆

1 of 1

[8+8]