Rr322304-bioprocess-engineering-ii

Set No. 1

Code No: RR322304

III B.Tech Supplimentary Examinations, Aug/Sep 2008 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Write short notes on:

[16]

(a) The aeration system (b) The agitator (c) Baffles (d) foam control. 2. Explain about Tracer pulse experiment to calculate conversion with respect to tanks-in-series model? [16] 3. List out the cardinal rules to be followed in design of a fermentor and its construction materials for successful operation? [16] 4. Write about the techniques employed in On-line analysis of Chemical factors? [16] 5. (a) Discuss in detail about ON-Line Sensor. (b) Discuss in detail about OFF-Line Analytical Methods

[8+8]

6. Explain in detail about the various stages in gene expression.

[16]

7. Discuss in detail about protein Engineering.

[16]

8. Discuss in detail about a highly structured single-cell model for growth of E.Coli on glucose-ammonium salts medium. [16] ⋆⋆⋆⋆⋆

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Set No. 2

Code No: RR322304

III B.Tech Supplimentary Examinations, Aug/Sep 2008 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Explain the basis for classification of Bioreactors and write in brief about them with an example? [16] 2. Write short notes on:

[16]

(a) Non-Ideal Tubular reactor (b) Non-Ideal CSTR. 3. List out the cardinal rules to be followed in design of a fermentor and its construction materials for successful operation? [16] 4. Write in detail about the methods employed in measuring the following process variables. [8+8] (a) Temperature (b) Pressure. 5. Explain the advantages gained by coupling process instruments to digital computers. [16] 6. Discuss in detail the control and information sequences in DNA guide the transcription and transaation process which result in gene expression. [16] 7. Write Short Notes On:

[16]

(a) Monoclonal antibodies. (b) Immunobiological Regulators. (c) Virus Vaccines. (d) Hormones. 8. Explain unit impulse response model. ⋆⋆⋆⋆⋆

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[16]

Set No. 3

Code No: RR322304

III B.Tech Supplimentary Examinations, Aug/Sep 2008 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. How cells grow in Continuous culture ? Explain about some specific devices employed for continuous culture ? [16] 2. Explain in detail the design of Continuous sterilization process ?

[16]

3. List out the cardinal rules to be followed in design of a fermentor and its construction materials for successful operation? [16] 4. How can you determine

[6+4+6]

(a) Cellular ATP content (b) Single cell characteristics (c) Assay of cells with and without plasmids. 5. (a) Discuss in detail about ON-Line Sensor. (b) Discuss in detail about OFF-Line Analytical Methods 6. Explain in detail about the various stages in gene expression.

[8+8] [16]

7. Discuss in detail about the formation of a hybridoma for production of monoclonal antibodies. [16] 8. Discuss in detail about a Morphologically structured kinetic model for Cephalosporin C production. [16] ⋆⋆⋆⋆⋆

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Set No. 4

Code No: RR322304

III B.Tech Supplimentary Examinations, Aug/Sep 2008 BIOPROCESS ENGINEERING-II (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. What are the different types of the bioreactors employed in Animal Cell Culture ? Explain about packed glass bead reactors and Perfusion cultures ? [16] 2. Write short notes on:

[8+8]

(a) One-parameter model (b) Two-parameter model 3. Write short notes on:

[8+8]

(a) Fed-batch reactors (b) Enzyme catalyzed reactions in CSTR. 4. Write in detail about the methods employed in measuring the following process variables. [8+8] (a) Temperature (b) Pressure. 5. (a) Discuss in detail about ON-Line Sensor. (b) Discuss in detail about OFF-Line Analytical Methods

[8+8]

6. Explain Holistic perspective on heterologous protein production with the baculovirus expression system. [16] 7. Discuss in detail about product formation kinetics.

[16]

8. Discuss in detail about a Morphologically structured kinetic model for Cephalosporin C production. [16] ⋆⋆⋆⋆⋆

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