Rr222303-enzyme-engineering-and-technology

Set No. 1

Code No: RR222303

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 ENZYME ENGINEERING AND TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. State the importance of coenzymes and cofactors in enzyme catalysis.

[16]

2. Describe the procedures of enzyme isolation from natural sources.

[16]

3. Explain substrate strain and transition state theory.

[16]

4. Derive Michaelis-Menten equation. State the importance of MM constant.

[16]

5. Discuss allosteric enzymes in terms of cooperativity.

[16]

6. Discuss the important criteria and prerequisite for selecting support for enzyme immobilization. [16] 7. Discuss about the effect of mass transfer resistance in immobilized enzymes. [16] 8. Enumerate the application of enzymes in analysis. ⋆⋆⋆⋆⋆

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Set No. 2

Code No: RR222303

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 ENZYME ENGINEERING AND TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Discuss enzyme classification in terms of four-digit classification number?

[16]

2. Describe the procedures of enzyme isolation from natural sources.

[16]

3. Discuss different types of enzyme specificity with suitable examples.

[16]

4. Derive Michaelis-Menten equation. State the importance of MM constant.

[16]

5. How Koshland-Nemethy-Filmer (KNF) model account for allosteric regulation. [16] 6. Discuss about the medical application of immobilized enzymes.

[16]

7. Give an account of different types of immobilized enzyme reactors.

[16]

8. What are the different types of transducer used in biosensors?

[16]

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Set No. 3

Code No: RR222303

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 ENZYME ENGINEERING AND TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. What are enzymes? Justify its role as biocatalysts.

[16]

2. Discuss the importance of spectrophotometry, spectrofluorimetry and luminometry in enzymes assay. [16] 3. Explain the energetics of enzyme-substrate complex formation.

[16]

4. Derive the general rate equation of Alberty for multi-substrate enzyme catalyzed reaction. [16] 5. Derive Monod-Wyman-Changeux model to account for cooperative binding. [16] 6. Discuss in detail about the across linking method for enzyme immobilization. [16] 7. Give an account of different types of immobilized enzyme reactors.

[16]

8. Enumerate the application of enzymes in analysis.

[16]

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Set No. 4

Code No: RR222303

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 ENZYME ENGINEERING AND TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. State the importance of coenzymes and cofactors in enzyme catalysis.

[16]

2. Describe the procedures of enzyme isolation from natural sources.

[16]

3. Discuss different types of enzyme specificity with suitable examples.

[16]

4. Derive the general rate equation of Alberty for multi-substrate enzyme catalyzed reaction. [16] 5. How Koshland-Nemethy-Filmer (KNF) model account for allosteric regulation. [16] 6. Discuss the important criteria and prerequisite for selecting support for enzyme immobilization. [16] 7. Give an account of different types of immobilized enzyme reactors.

[16]

8. Enumerate the application of enzymes in analysis.

[16]

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