RESULTS Specimen used: Siling labuyo Solvent System used: DCM:Hexane
Rf value =
Column Chromatography Column Chromatography yielded four eluates from the extraction of the color components of Siling Labuyo. Color of Component
The Rf values were computed using this formula:
Volume of eluates in drops
1 2 Yellow 80 drops 3 Orange 69 drops 4 Light orange 70 drops Table 1. Shows the results from the column chromatography. (Three) eluates produced in the experiment and its volume which were in the form of drops. The first eluate which is yellow produced a total of 80 drops. The second eluate which is orange produced 69 drops. While the third eluate is a light orange and produced 70 drops. Thin Layer Chromatography Color of Distance component from the origin (x) 1 5.8 cm 2 Yellow 4.3 cm 3 Orange 3.5 cm 4 Light 1.1 cm Orange Table 2. Shows the distance in the precoated TLC plates from the origin (x) after performing the thin layer chromatography.
distance of solute____ distance of solvent front
Color of Component
Rf value
1 2 Yellow 0.74137931 3 Orange 0.60344828 4 Light Orange 0.18965517 Table 3. Rf values of color of component extracted from Siling Labuyo.