Results Of Dmsa Treatment Study

Results of DMSA Treatment Study James Adams, Liz Geis, Matthew Baral, Jessica Mitchell, Julie Ingram, Andrea Hensley, Sanford Newmark, Eva Gehn, Bob Rubin, Warren Tripp, Ken Mitchell, Jeff Bradstreet, Jane El-Dahr Southwest College of Naturopathic Medicine Funded by Wallace Foundation and Autism Research Institute

Current study Goal: Determine if DMSA/glutathione therapy helps children with autism. Phase 1: 9 doses of DMSA over 3 days, 10 mg/kgdose; collect urine at baseline, after 1st dose, and after 9th dose; daily doses of “glutathione” - if high toxic metal excretion, continue to phase 2 Phase 2: 3 month, double-blind, 1 round-controlled treatment study; 3 days on DMSA, 11 days off; repeat 6x 82 children enrolled 65 completed phase 1 41 completed phase 2

Phase 1 • • • •

Started with 82 participants 1 did not qualify due to elevated liver enzymes 4 stopped after physical exam 11 stopped after initial blood draw (2 could not collect baseline urine) • 1 collected urine after DMSA but did not send to lab (busy family) • 65 collected urine after DMSA and sent to lab for testing

Toxic Metal Excretion after DMSA – 1st & 9th dose % changes in median values (N=63)

Loss of essential minerals Potassium: 1st dose – lost 27% of RDA; 9th dose: lost 12% of RDA; So 1st day probably lost about 75% of RDA, 2nd day about 55% of RDA, and 3rd day about 40% of RDA; p<.0000000001 Equivalent to loss of about 7 bananas worth of potassium over 3 days However, blood levels normal when tested 3-4 weeks later, so no long-term effect Chromium: 1st dose – lose 45% of RDA; 9th dose – lose 15% of RDA; p=0.0005; so, several days of DMSA results in several days loss of chromium

Loss of essential minerals (cont.) Molybdenum – decreased excretion by 6% on 1st day, 30% on 9th dose. Probably not a concern. Sodium: 1st dose – increased excretion of 30%, normal by 9th dose; not a concern Copper and Zinc: Increased excretion, but less than 1% of RDA Vanadium – small loss (about 1/3 of daily intake) Little change: calcium, phosphorus, magnesium, sulfur, selenium Conclusion: DMSA causes some loss of potassium and chromium, small loss of vanadium; excretion of other minerals is unimportant Recommendation: when using DMSA, eat extra fruits/vegetables for potassium (cannot supplement it), and supplement chromium and possibly vanadium.

Change in Glutathione

Change in Glutathione

500 250 0 -250 0

300

600

900

-500 -750 Initial Glutathione

Initial Glut: 31-1033 - many lower/higher than adult RR of 427-714 Final Glutathione: 355-695

- almost all within reference range

For low RBC glutathione, DMSA greatly increased values to normal For high RBC glutathione, DMSA reduced values towards normal

Why are some RBC Glutathione unusually low and high? Initial glutathione correlates with Pb-9 (.25), Sbb (0.26), Cd-9 (0.30), Al-9 (0.29), and inversely correlates with Hg-9 (-.27). Hypothesis: body responds to Pb, Sb, Cd, Al by making more glutathione. But, mercury blocks production of glutathione, and decreases it.

Why does 1 round of DMSA normalize glutathione? • Change in glutathione correlates with Hg-9 (0.31), and inversely correlates with W-9 (-0.45) and Cd-9 (-0.47). • So, removing some mercury increases low glutathione, and removing tungsten and cadmium reduces high glutathione.

Changes in Blood Chemistry after 1 round of DMSA (measured 3-6 weeks later, n=41) Major Tests – no major problems, possible improvement of kidney function White Blood Cell -3% Platelet Count -6%, p=0.02 (12/42 high, 1 low; then 6/42 high, 0 low) Lymphocytes -4% Potassium +1% ALT (liver enzyme) +4% AST (liver enzyme) 0% BUN/creatinine -6%, n.s. (17/42 high, 0 low, then 14/42 high, 1/42 low) Creatinine -2% (0 high, 8/42 low, then 0 high, 9/42 low) Other changes (none were statistically significant) Basophils +24% Bilirubin -15%, n.s.

Suggests no major safety concerns for 1 round; Platelet levels improve (less inflammation); BUN/creatinine often high, since creatinine low, BUN high

Transition to Phase 2 • 8 children not allowed to continue due to low excretion of toxic metals • 1 child not allowed to continue due to extremely high lead • 1 family left on extended travel • 1 family wanted to try other treatments • 1 child discontinued due to extended use of antibiotics due to urinary tract infections • 1 family too busy/overwhelmed • 1 child had extreme anxiety over blood draws • 1 wanted to start private treatment (avoid risk of placebo) • 1 had mild adverse reactions (lethargy, increased appetite) 49 continued to Phase 2

Phase 2 : Randomized, double-blind, placebo-controlled • 2 dropped due to family reasons (parents busy, parent died) • 2 dropped due to lack of improvement • 4 dropped due to behavior problems – – – –

Mild – slept very little, but not tired (on DMSA) Moderate – behavior worsened, more stimming – (on placebo) Moderate – behavior worsening, regressing – (on placebo) ???? – parents reported gain and lose skills, similar pattern for 2 years prior to study, but ADOS scores improved (on DMSA)

• 41 finished full study, including 4 in treatment group who finished early due to low excretion after 3rd round

Long-term Effect of DMSA on Urinary Excretion of Toxic Metals – Changes in Median Values Treatment Group Only (n=26)

Summary of long-term metal excretion • For 5 children, DMSA treatment resulted in substantial decrease in excretion of lead, so they were only treated for 3-4 rounds • For most of the children receiving DMSA (19), lead excretion continued at a high level. • Moderate excretion of mercury, tin, thallium. • Al excretion initially decreased, then increased.

Changes in Blood Chemistry after 7 rounds of DMSA n=21 No major problems; platelets improved; kidney function remains abnormal Platelets

-18%, p=0.05 (initially 10 high, 1 low to 4 high, 0 low – improved!)

White Blood Cell low) Lymphocytes Potassium ALT (liver enzyme) AST (liver enzyme) BUN/creatinine (kidney)

-7% n.s. (initially 1 high, 0 low; then 1 high, 1 0% +0% +3% - initially 1 high, then all ok -6% - all normal +8% - initially 10 high, then 9 high, 1 low

Statistically Significant changes: Triglycerides: +47%, p=0.06 (trend) - initially zero high, then 2 high Other changes (not significant): Eosinophils: -19%, p=0.08 3 high, then 2 high Basophils +50%, n.s. - all normal

Changes in blood chemistry after 1 round DMSA, 7 rounds placebo Platelets: -13%, p=0.01 initially 1 high, then all normal; measured 3-4 months after treatment, so effects are longlasting Similar to 7 dose case (-18%) Nothing else significant (no long-term effects; safe)

Regression analysis of Platelets Change in platelet level could be partially explained (adjusted R2=0.41, p=0.02) with major factors being excretion of Thallium (p=0.002), Arsenic (p=0.01), Cadmium (p=0.03), and change in glutathione (p=0.04)

Evaluations of Autism Symptoms Parents • Severity of Autism Scale • ATEC – parents • PDD-BI • Global Impressions Research-certified professionals did ADOS

Severity of Autism Scale 0-10 point scale • 7 round group: -18% (improvement) • 1-round group: -18% (improvement) Significant improvement in both groups.

ATEC Results Scale

7 rounds

1 round

I. Speech/Language Comprehension

-21%

-13%

II.Sociability

-27%

-25%

III. Sensory/ Cognitive Awareness

-27%

-26%

IV. Health/ Physical/ Behavior

-28%

-15%

ATEC Total

-26%

-19%

Both groups significantly improved 7 round group improved more in Language, Physical Health, and Total ATEC, but not statistically significant

Pervasive Developmental Disorders Behavior Inventory (PDD-BI)

Significant improvement in both groups

Overall Impressions - Results Based on parent evaluations on final day of study 7 point scale 3=much better 2=better 1=slightly better 0=same -1=slightly worse -2=worse -3=much worse

Parent Global Impressions

Parent Impressions- Overall

ADOS exam • Autism Diagnostic Observation Schedule • Based on 1-hour standardized interaction with child by ADOS-certified professional • One of the “gold standards” for evaluating severity of autism

ADOS results 7 rounds

1 round

Communication

-9%

-11%

Social Interaction

-10%

-2%

Play/Creativity

-5%

0%

SBRI (stereotyped behavior and restricted interests) -9% -2% ADOS less sensitive than other instruments (developed to diagnose autism, not monitor changes). Overall, 7-round group improved slightly more, but not significant difference;

Summary of Autism Evaluations Significant improvements on all scales for both groups 7 dose group had slightly better improvements on ATEC and ADOS, but not statistically significant Similar improvements in PDD-BI, SAS, Global Impressions Since most kids still excreting high amounts of lead after 7 rounds, suggests longer treatment needed to reduce lead excretion. Question: would longer treatments result in more behavioral improvements?

Why little difference between 7 rounds and 1 round? Hypothesis 1: DMSA ineffective on autism symptoms (placebo effect). Hypothesis 2: 1st round of DMSA normalized glutathione and improved platelet levels, so that further treatment had little additional effect. Correlations of behavioral improvement with biochemical changes suggest hypothesis 2 is correct.

Overall change in autism severity, including both 7-round and 1-round groups % Improved

% No Change

% Worsened

ATEC

96%

2%

2%

SAS

61%

33%

6%

ADOS (Comm. + 68% Social)

15%

17%

PDD-BI 75% (modified Autism Composite)

2%

22%

Parent Global Impression

86%

8%

6%

Average of all 5 assessments

77%

12%

11%

Regression Analysis – Severity of Autism Compare severity of autism with glutathione and metal tests adjusted R2 Most significant metals • ATEC: 0.22 p=0.003 Pb-9, Sb-b • SAS: 0.36 p=0.002 Pb-b • PDD-BI: 0.25 p= 0.004 Sb-9, W-b, Sn-9 • ADOS: 0.49 p=0.0003 Hg-b, Al-b, Hg-9 All four scales of autism severity can be partially explained in terms of heavy metal excretion, with a very high statistical significance. Suggests 22-49% of autism severity appears to be due to toxic metals, especially lead, antimony, and mercury.

Regression Analysis – Improvement Compare improvements in severity of autism with glutathione and metal tests adjusted R2 Most significant metals • ATEC: 0.44 p=0.006 Hg-9, As-9 • SAS: 0.57 p=0.002 Tl-9, Pb-9, glut change • PDD-BI: 0.75 p=0.0006 Tl-9, As-9, glut change, Pb-9, Sb-9 • ADOS: 0.28 p=0.02 As-9, Al-9 Changes in all four scales can be partially explained (28-75%) in terms of urinary excretion of metals Arsenic, thallium, lead, and change in glutathione are most important When glutathione increases, symptoms generally decrease. When metal excretion increases, symptoms generally decrease

Effect of Age on Improvement Slight negative correlation suggests that older children possibly tended to improve slightly more than younger children (not significant) Test ATEC SAS PDD-BI ADOS

Correlation with age -.20 -.19 -.12 -.06

Conclusions - benefits • DMSA greatly increases excretion of lead, and some increase in excretion of tin, mercury, thallium. • 1 round of DMSA dramatically normalized glutathione levels for at least 1-2 months, and helped normalize platelet levels (marker of inflammation) for at least 4 months

Conclusions - safety • DMSA increases excretion of potassium and chromium; suggests need for more vegetables/fruit and modest chromium supplementation during chelation • DMSA had little effect on other essential minerals • DMSA had no adverse effect on liver enzymes, kidney function, or complete blood count (CBC) • DMSA possibly raised triglycerides – concern to watch for • Check cysteine levels, since 90% of DMSA is excreted bound to 1-2 cysteine.

Conclusions – Effect on Symptoms • Both groups had significant improvements on autism scores • 7-round group had slightly more improvements on ATEC and ADOS, but not significant • More improvement in those with low glutathione and high initial metal excretion – strong correlations and regression analysis strongly suggest that improvement is real (not just placebo effect)

Bottom Line Toxic metals (especially lead, antimony, and mercury) account for 22-49% of autism severity. DMSA is a safe way to remove toxic metals, normalize glutathione, normalize platelets/inflammation Correlation of improvement in autistic symptoms with glutathione and metal excretion suggests DMSA did result in reduction of some symptoms of autism. Longer treatment needed by most children to decrease lead levels – unknown if longer treatment might provide additional behavior benefit. Double-blind, placebo-controlled study of DMSA needed Other chelators may be needed for mercury and other metals (arsenic, antimony). More research should be done!

Questions • Will longer treatment with DMSA result in more benefit? – Probably, but need to study

• How to test for and remove antimony? – DMPS, somewhat Ca-EDTA

• How to test for and remove mercury? – DMPS challenge, urinary porphyrins, other

Acknowledgements • Many children and parents who participated in the study • ARI and Wallace Foundation for funding • Doctor’s Data for urine testing • Immunosciences for RBC glutathione and immune testing