Rar05322301-analytical-techniques-in-bio-technology-set1

RA

Code No: RAR05322301

III B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 ANALYTICAL TECHNIQUES IN BIO TECHNOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ????? 1. Explain the following terms associated with Fluorescent Microscope. (a) Barrier filter (b) Heat filter (c) Fluorochromes (d) Exciter filter.

[4+4+4+4]

2. What is the difference between a TEM and a SEM?

[16]

3. How is blood sugar measured?

[16]

4. What are the factors affecting electrophoretic mobility of any molecule?

[16]

5. Explain the basic principle behind all forms of Chromatography

[16]

6. How is PCR used to sequence DNA?

[16]

7. Write short notes on (a) Rocket Immunoelectroforesis (b) Radial Immunoassays.

[8+8]

8. Explain (a) Substrate uptake in absence of product formation. (b) Substrate uptake with product formation. ?????

1 of 1

[8+8]