R05222303-molecular-biology

Set No. 1

Code No: R05222303

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Represent a melting curve of a double helix DNA and explain it in detail?

[16]

2. Write a note on the famous Messelson & Stahl experiment on proving semi conservative mode of DNA replication. [16] 3. What is the mechanism & importance of “proof reading” function of DNA polymerase in DNA replication? [16] 4. With respect to tRNA structure, explain: (a) Stems& loops (b) Anticodon arm.

[2 × 8 = 16]

5. Which are the different types of rRNA associated with eukaryotic ribosome? How are these synthesized? [16] 6. How are Wobble hypothesis and the genetic code correlated with each other? [16] 7. How is the process of eukaryotic process of translation different from prokaryotic process. [16] 8. With context to mutations, what is meant by: (a) Base substitutions (b) Insertions and deletions. ⋆⋆⋆⋆⋆

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[2 × 8 = 16]

Set No. 2

Code No: R05222303

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Which form of DNA exists as the natural form present in living cells? Explain in detail. [16] 2. What is meant by “rolling circle” form of DNA replication? Give an example. [16] 3. What is meant by ‘end replication problem’ in DNA synthesis? Discuss the role of telomase in it. [16] 4. How are eukaryotic and prokaryotic RNA polymerases different from each other? [16] 5. What is the relevance of intron and exon sequences with respect to the final mRNA formation in eukaryotic posttranscriptional processing? [16] 6. Explain how the genetic information present in the mRNA is decoded into amino acid sequence during the process of translation? [16] 7. What are post transnational modifications? Give some examples.

[16]

8. Briefly explain the different DNA repair mechanisms existing in a cell.

[16]

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Set No. 3

Code No: R05222303

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. what is the primary structure of DNA? Explain in detail.

[16]

2. Write a note on the famous Messelson & Stahl experiment on proving semi conservative mode of DNA replication. [16] 3. Discuss about the various enzymes involved in carrying out the process of the DNA replication. [16] 4. Discuss about the different types of RNA and the RNA Polymerases, which are involved in synthesizing them. [16] 5. Write a note on how tRNA is produced by processing larger pre-tRNA transcript. [16] 6. How are Wobble hypothesis and the genetic code correlated with each other? [16] 7. Explain how conjugation is a process through which genetic recombination is possible in bacteria? [16] 8. With reference to acting as mutagens, explain: (a) Uv irradiation (b) Ionizing radiation. ⋆⋆⋆⋆⋆

1 of 1

[2 × 8 = 16]

Set No. 4

Code No: R05222303

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 MOLECULAR BIOLOGY (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. What are the main functions of DNA in a living cell?

[16]

2. How does DNA replication occur in Mitochondria DNA?

[16]

3. What are Telomers in DNA? How do they replicate.

[16]

4. Write notes on: (a) Prokaryotic RNA polymarase (b) Eukaryotic RNA polymarase.

[2 × 8 = 16]

5. Explain the sequence by which introns are removed from a eukaryotic pre-mRNA. [16] 6. Elaborate the statement “Genetic code is a triplet, redundant, nonoverlapping & comma free code”. [16] 7. Write short notes on:

[2 × 8 = 16]

(a) tRNA as an adaptor molecule (b) Difference between prokaryotic and eukaryotic ribosomes. 8. With reference to acting as mutagens, explain: (a) Uv irradiation (b) Ionizing radiation. ⋆⋆⋆⋆⋆

1 of 1

[2 × 8 = 16]