QUIZ REVIEW!
Freddy, Avery, Hershey & Chase (WANTED to figure out what?) (HOW did they figure it out?) (WHAT did they discover?)
Remember this?
Fredrick Griffith: Wanted to: Figure out a cure for pneumonia
The How: Fredrick Griffith’s experiment HOW: •
Two strains of bacteria; virulent and avirulent
•
Mixed dead virulent strain with live avirulent strains
•
Injected this mix into a mouse- the mouse should live since virulent strain is dead
Fredrick Griffith WHAT DID HE DISCOVER?? 1. there was transforming factor that remained AFTER the bacteria were dead 2. he wasn’t sure what transformed it yet…
The WHY: of Oswald “Ozzy” Avery • •
He wanted to know MORE about Griffith’s experiment “What was the transforming element?” Proteins? Lipids? Carbohydrates? Nucelic acid?
HOW Ozzy Avery figured it out 1. Took heat killed bacteria like Griffith 2. Wanted to isolate the transforming element 3. Destroyed protein, lipids, carbohydrates, nucleic acid.
OZZY found out that: • • • •
Protein didn’t transform Carbohydrates didn’t transform Lipids didn’t transform DNA did!
Hershey and Chase: WANTED to find out: What part of a bacteriophage made more bacteriophages?
The how: Hershey and Chase 1. Phages made of two things: protein and DNA 2. Radioactive marked: Sulfur in protein and Phosphorous in DNA 3. Let the phages go and attack bacteria!
Hershey & Chase found: • • •
•
Protein was not injected into bacteria DNA was injected into bacteria DNA was replicated, and the next generation of phages had the radioactive element! DNA WAS the TRANSFORMING FACTOR!
Meiosis!?
STRUCTURE!?