Purification Table
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Population LT-HSC NK Cells B-Cells naïve CD4+ T-Cells activated CD4+ T-Cells naïve CD8+ T-Cells activated CD8+ T-Cells Granulocytes Monocytes Nucleated Erythrocytes
Selection Criteria SP+ c-Kit+ Sca-1+ Lin Nk1.1+ CD3CD19+ 33D1CD4+ CD25- CD69Isolated 8-11 Hours After Stimulation CD4+ CD25+ CD69+ CD8+ CD25- CD69Isolated 8-11 Hours After Stimulation CD4+ CD25+ CD69+ Gr-1+ 7/4 clone+ Lin- (CD2,CD5,B220,F4/80,ICAM-1,Ter-119) Mac-1+ FSC and SSC properties Ter119+ Lin- (CD3, CD4, CD8, Mac1, Gr1, B220)
Tissue WBM PB Spleen Spleen Spleenocytes treated with ConA Spleen Spleenocytes treated with ConA WBM PB WBM
Purity 0.99 0.99 0.99 0.98 0.99 0.99 0.99 0.99 0.96 0.98
Selection Criteria SP+ c-Kit+ Sca-1+ Lin Nk1.1+ CD3CD19+ 33D1CD4+ CD25- CD69Isolated 8-11 Hours After Stimulation CD4+ CD25+ CD69+ CD8+ CD25- CD69Isolated 8-11 Hours After Stimulation CD4+ CD25+ CD69+ Gr-1+ 7/4 clone+ Lin- (CD2,CD5,B220,F4/80,ICAM-1,Ter-119) Mac-1+ FSC and SSC properties Ter119+ Lin- (CD3, CD4, CD8, Mac1, Gr1, B220)
Tissue WBM PB Spleen Spleen Spleenocytes treated with ConA Spleen Spleenocytes treated with ConA WBM PB WBM
Purity 0.99 0.99 0.99 0.98 0.99 0.99 0.99 0.99 0.96 0.98
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