Protein Synthesis
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From Genes to Proteins
DNA and RNA differ in 3 ways RNA
DNA
Single-stranded
Double-stranded
Ribose (sugar)
Deoxiribose (sugar)
Uracil (base) bonds to Adenine
Thymine (base) bonds to Adenine
The Flow of Information
The Flow of Information
Transcription This is the first step in Protein Synthesis: 1. The instructions are transferred (“transcribed”) to an RNA molecule.
To sum up Transcription…
Info transferred from DNA to RNA
What is the Enzyme involved in Transcription? Answer RNA Polymerase
Transcription has 3 steps… 1 – RNA Polymerase binds to the gene’s promoter (DNA) (like a starting line in a race).
2 – RNA Polymerase UNWINDS the DNA molecule. The DNA nucleotides are exposed.
3 – RNA Polymerase (yeah, that same enzyme again…) adds complimentary nucleotides to separated DNA strand. ** Remember RNA has Uracil instead of Thymine for a base.
The RNA Polymerase will continue transcription until it reaches the “stop signal” on the DNA molecule (like a finish line). Then the RNA strand is released and goes on to the next step… Translation
Transcripti on
Transcription
3 types of RNA
mRNA (messenger RNA)
rRNA (ribosomal RNA)
tRNA (transfer RNA)
Messenger RNA
Delivers information to the site of Translation.
mRNA instructions are written in “3-nucleotide” sequences. These sequences are called codons. Ex. See pg. 209 UUU, CUG, ACU, etc. There are 64 possible codons.
Translation
Remember what happens in Transcription?
DNA to RNA
In Translation…RNA is coded for Amino Acids.
Translation takes place in the Cytoplasm. tRNA (Transfer RNA) molecules carry single amino acids. They also have an OPPOSITE “3nucleotide” sequence called
rRNA (Ribosomal RNA) molecules are like assembly lines they carry: 1 mRNA 2 tRNA
7 Steps in Translation 1 – mRNA start codon starts the process at the P site. 2 – the next tRNA bonds to the next codon at the A site. 3 – A & P are holding 2 tRNA’s…a peptide bond is formed between 2 amino acids.
4 – tRNA detaches from P-site, leaves behind amino acid, leaves Ribosome. 5 – tRNA at A-site moves to the P-site. Now a new codon is ready at the Asite for another tRNA.
6 – tRNA detaches from P-site, leaves behind amino acid, leaves ribosome. 7 – (Steps 2 – 6 repeat until a stop codon is reached). Ex. UAG, UAA, UGA. A new protein is then released into the cell.
Translatio n
Translation
DNA and RNA differ in 3 ways RNA
DNA
Single-stranded
Double-stranded
Ribose (sugar)
Deoxiribose (sugar)
Uracil (base) bonds to Adenine
Thymine (base) bonds to Adenine
The Flow of Information
The Flow of Information
Transcription This is the first step in Protein Synthesis: 1. The instructions are transferred (“transcribed”) to an RNA molecule.
To sum up Transcription…
Info transferred from DNA to RNA
What is the Enzyme involved in Transcription? Answer RNA Polymerase
Transcription has 3 steps… 1 – RNA Polymerase binds to the gene’s promoter (DNA) (like a starting line in a race).
2 – RNA Polymerase UNWINDS the DNA molecule. The DNA nucleotides are exposed.
3 – RNA Polymerase (yeah, that same enzyme again…) adds complimentary nucleotides to separated DNA strand. ** Remember RNA has Uracil instead of Thymine for a base.
The RNA Polymerase will continue transcription until it reaches the “stop signal” on the DNA molecule (like a finish line). Then the RNA strand is released and goes on to the next step… Translation
Transcripti on
Transcription
3 types of RNA
mRNA (messenger RNA)
rRNA (ribosomal RNA)
tRNA (transfer RNA)
Messenger RNA
Delivers information to the site of Translation.
mRNA instructions are written in “3-nucleotide” sequences. These sequences are called codons. Ex. See pg. 209 UUU, CUG, ACU, etc. There are 64 possible codons.
Translation
Remember what happens in Transcription?
DNA to RNA
In Translation…RNA is coded for Amino Acids.
Translation takes place in the Cytoplasm. tRNA (Transfer RNA) molecules carry single amino acids. They also have an OPPOSITE “3nucleotide” sequence called
rRNA (Ribosomal RNA) molecules are like assembly lines they carry: 1 mRNA 2 tRNA
7 Steps in Translation 1 – mRNA start codon starts the process at the P site. 2 – the next tRNA bonds to the next codon at the A site. 3 – A & P are holding 2 tRNA’s…a peptide bond is formed between 2 amino acids.
4 – tRNA detaches from P-site, leaves behind amino acid, leaves Ribosome. 5 – tRNA at A-site moves to the P-site. Now a new codon is ready at the Asite for another tRNA.
6 – tRNA detaches from P-site, leaves behind amino acid, leaves ribosome. 7 – (Steps 2 – 6 repeat until a stop codon is reached). Ex. UAG, UAA, UGA. A new protein is then released into the cell.
Translatio n
Translation
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