Power Supply Selection Guide

Power Supplies Bio-Rad offers a complete line of power supplies for the most demanding of electrophoretic techniques. These power supplies are lightweight and easy to program, and are certified to IEC 1010-1 — the most rigorous international safety standard — to ensure the highest personal and environmental protection. Use the selection guide to choose the power supply that best suits your electrophoresis needs.

Power Supply Selection Guide According to Electrophoretic Technique Technique and Recommended Apparatus

Typical Conditions* (Initial) W V mA

Typical Conditions* (Final) W V mA

Typical Run Time

PowerPac™ Power Supply

Laemmli (SDS), O’Farrell Second Dimension (SDS) 160 x 160 x 1.5 mm, 2 gels PROTEAN® II xi cell 183 x 200 x 1.5 mm, 2 gels PROTEAN II XL cell 133 x 87 x 1.0 mm, 2 gels Criterion™ cell ® 83 x 73 x 1.0 mm, 4 gels Mini-PROTEAN Tetra cell

— — — —

100 100 200(C) 200(C)

35(C) 35(C) 200 120

— — — —

350 350 200(C) 200(C)

35(C) 35(C) 80 60

5 hr 5 hr 55 min 45 min

HV or Universal HV or Universal Basic or HC Basic or HC

High-Throughput Electrophoresis 200 x 205 x 1.0 mm, 12 gels PROTEAN® Plus Dodeca™ cell

—

200(C)

1,000

—

200(C)

350

6 hr

HC or Universal

—

500

480(C)

5 hr

Universal

Gel or Tube Size, Qty (W x L x Thickness)

PROTEAN II xi/XL multi-cell

160 x 200 x 1.5 mm, 6 gels

—

150

480(C)

Criterion Dodeca cell

133 x 87 x 1.0 mm, 12 gels

—

200(C)

1,000–1,400

—

200(C)

400–500

55 min

HC or Universal

Mini-PROTEAN 3 Dodeca cell

83 x 73 x 1.0 mm, 12 gels

—

200(C)

600

—

200(C)

360

45 min

HC or Universal

—

800(C)

3.5

—

800(C)

<1

16 hr

HV or Universal

—

750(C)

1

—

750(C)

<1

3–4 hr

HV or Universal

Preparative Electrofocusing 60 ml focusing chamber Rotofor® cell 18 ml focusing chamber Mini Rotofor cell

15(C) 12(C)

500 500

24 24

15(C) 12(C)

1,200 1,200

10 10

4 hr 4 hr

HV HV

Preparative PAGE Model 491 prep cell Mini prep cell

— —

10(C) 1(C)

300 200

40 5

10(C) 1(C)

400 300

30 3

3–8 hr 3–8 hr

HV or Universal HV or Universal

Protein Electroelution Model 422 electro-eluter

6 samples

—

200

60(C)

—

150

60(C)

3–4 hr

Basic, HV, or Universal

Whole gel eluter

160 x 140 mm or larger

15

200(C)

—

15

200(C)

—

20 min

Basic

Mini whole gel eluter

55 x 65 mm or larger

10

100(C)

—

10

100(C)

—

20 min

Basic

— — —

100(C) 200(C) 450(C)

6 6 4

— — —

100(C) 200(C) 450(C)

4 4 1

15 min 15 min 1 hr

HV or Universal

DNA Restriction Analysis (Horizontal Mode) 150 x 200 x 5 mm Sub-Cell® GT cell 70 x 100 x 5 mm Mini-Sub® Cell GT Wide Mini-Sub Cell GT 150 x 100 x 5 mm

— — —

80(C) 50(C) 50(C)

55 25 35

— — —

80(C) 50(C) 50(C)

60 30 40

4 hr 2 hr 2 hr

Basic or HC Basic Basic

DNA Sequencing Sequi-Gen® GT system

Isoelectric Focusing (IEF), O’Farrell First Dimension 150 x 1.5 mm tubes, PROTEAN II xi cells 4 (minimum) or Model 175 tube cell Mini-PROTEAN II tube cell

75 x 1.0 mm tubes, 8 (minimum)

Polyacrylamide Analytical Electrofocusing 125 x 65 x 0.4 mm Model 111 mini IEF cell

80(C)

1,850

30

80(C)

1,850

30

2–4 hr

HV

Single-Stranded Conformation Polymorphism (SSCP) 210 x 400 x 0.4 mm Sequi-Gen GT system

40(C)

1,800

20

40(C)

1,800

20

2–3 hr

HV

Microsatellite Mapping Sequi-Gen GT system

210 x 400 x 0.4 mm

50(C)

2,100

25

50(C)

2,100

25

2–3 hr

HV

Mutation Detection DCode™ system

100 x 75 x 1.0 mm, 2 gels

—

130(C)

—

—

130(C)

—

2.5 hr

Basic or HV

83 x 73 x 0.75 mm, 2 gels

—

100(C)

250

—

100(C)

450

1 hr

HC

133 x 87 x 1.0 mm, 2 gels 133 x 87 x 1.0 mm, 2 gels

— —

100(C) 100(C)

250 650

— —

100(C) 100(C)

450 1,600

1 hr 30 min

HC HC

200 x 160 x 1.5 mm, 1 gel 200 x 160 x 1.5 mm, 1 gel 200 x 160 x 1.5 mm, 1 gel 265 x 280 x 1.5 mm, 3 gels

— — — —

60(C) 100–150(C) 50–100(C) 100(C)

210–250 1,000–1,600 1,600 3,000

— — — —

60(C) 100–150(C) 50–100(C) 100(C)

210–250 1,000–1,600 1,600 3,000

5 hr 1–5 hr 30 min 30 min–1 hr

HC HC HC HC

250 x 180 x 1.5 mm 150 x 150 x 6 mm

— —

15(C) 15

500 650(C)

— —

15(C) 25

200 650(C)

15–30 min 10–30 min

HC HC

Western Blotting Mini Trans-Blot® cell Criterion blotter Wire electrodes Plate electrodes Trans-Blot® cell Wire electrodes Plate electrodes High-intensity transfer Trans-Blot® Plus cell Semi-Dry Blotting Trans-Blot SD cell Protein DNA/RNA

380 x 500 x 0.4 mm

* (C) = constant; typical conditions are listed as guidelines only and can vary based on sample, buffers, etc.