Pdt-intracellular Pathogen Targeting

Targeting internalized bacteria using PDT

Host cell bearing intracellular bacteria

Binding of photosensitizer to bacteria/sub-cellular components

Photosensitization using metheylene blue (photosensitizer)

Irradiation using appropriate wavelength

Internalization of Photosensitizer

Bacteria eliminated from host cell Cytotoxicity?

Effect of sensitization time A

The association of MB with THP1 cells increased with increasing concentrations (A) and sensitization time (B). B After 20 minutes of sensitization, ~ 4.4135X1012 molecules of MB are associated with each THP1 cells

Effect of sensitization time: Damage to THP1 cells A

Mitochondrial and cell membrane damage on THP1 cells induced by PDT B

1

2

DNA damage in THP1 cells induced by PDT 3

4

1.

Control

2.

H2O2

3.

Irradiation

4.

Sensitization

5.

PDT

5

E

5

Effect of sensitization time:

bacterial killing vs membrane integrity of THP1 4

erfacti b m u logn

Membrane damage in THP1 cells vs bacterial 3kill when PDT was conduced on THP1 with intracellular bacteria.

2

1

T