Outline_ion Channels And Receptors

Ion channels and receptors: structure, mechanisms and regulation Contact: Min Li, Ph.D. Department of Neuroscience & High Throughput Biology Center BRB 319 Johns Hopkins School of Medicine 733 North Broadway Baltimore, MD 21205 [email protected] Outline: 1. Background • General biology of membrane proteins • Receptors, channels and transporters • Ionic gradients in animal cells 2. Molecular • • • •

biology of ion channels Architecture and functional domains of ion channels Classification of ion channels Regulation and gating of ion channels Methods for studying ion channels

3. Cell biology of ion channels • Subunit assembly of ion channels • Membrane trafficking and regulation • Molecular coupling of ion channels and signaling proteins 4. Ion channels, human diseases and therapeutics • CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) • KCNQ potassium channels (epilepsy) • hERG (cardiac safety pharmacology) Discussion paper: Mitcheson, J. S., Chen, J., Lin, M., Culberson, C., and Sanguinetti, M. C. (2000). A structural basis for drug-induced long QT syndrome. Proc Natl Acad Sci U S A 97, 12329-12333.