New Enzyme Table

Enzyme

Substrate

Product

Use

L.O.D. (µM)*

Reference

Acetylcholinesterase

Acetylcholine

Choline, Acetic Acid

Measure Cholinewith Choline oxidase

0.18 ± 0.02

Burmeister, JJ et al., 2008

0.04 ± 0.01

N/A

Adenosine Ascorbate oxidase

Ascorbate, O2

Dehydroascorbate

Remove Ascorbate

N/A

Catalase

H2O2

H2O, O2

Remove H2O2

N/A

Choline Oxidase

Choline, O2

H2O2, Betaine

Measure Choline

0.33 ± 0.03

Parikh, V et al., 2004

Glucose oxidase

Glucose, O2

H2O2, Gluconic Acid

Measure Glucose

640.0 ± 140.0

Burmeister, JJ et al., 2001

L-glutamate oxidase

L-glutamate, O2

H2O2, αketoglutarate

Measure L-glutamate

0.52 ± 0.03

Burmeister, JJ et al., 2002

NADPH, H+, succinate, Lglutamate

Measure GABA with L-glutamate oxidase

4.5 ± 0.20

N/A

GABase

GABA, H2O, NADP+ α-ketoglutarate

Lactate oxidase

Lactate, O2

H2O2, Pyruvate

Measure Lactate

78.0 ± 13.0

Burmeister, JJ et al., 2005

Exclusion Layer

Substrate

Product

Use

L.O.D. (µM)*

Reference

Nafion® & 1,3-Phenylenediamine

Dopamine

Dopamine o-quinone

Measure Dopamine

N/A

Serotonin??? O2, H2O

H2O2, OH-

*Values Reported as Mean ± Standard Error of the Mean

Measure Oxygen

1.7 ± 0.11

N/A