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12265 SW 4TH TERR • MIAMI, FL 33184 P H O N E 3 0 5 - 3 2 2 - 3 1 9 3 • E - M A I L F E R R E RC @ B U C M A I L . B A R RY. E D U CHRISTINA
FERRER
EDUCATION 08/2003 – 05/2007 B.S., University of Central Florida Orlando, Fl Major: Liberal Studies, Health and Physical Sciences; Minor: Biology 08/2007 – 05/2009 M.S., Barry University Shores, Fl Major: Biomedical Sciences 08/2007 – 05/2008 MT, Miami Dade College Major: Medical Laboratory Technology
Miami
Miami, Fl
RELATED WORK EXPERIENCE 03/2008 – Present Laboratory Research Intern Florida International University, School of Medicine, Molecular Biology Laboratory Miami, Fl • PI: Dr. Rene J. Herrera, PhD; phone # (305) 348-1258 • Doctoral Fellow: Jason A. Somarelli o Using antibodies, generated through in vitro phage display technology, for the recognition of an FKBP45 peptide. o Extraction of antibodies from E.coli in order to express, isolate and purify the anti-FKBP45 peptide antibodies from phage display clones o ELISA detection to test the affinity of anti-FKBP45 peptide antibodies o Western blot detection to test the specificity of anti-FKBP45 peptide antibodies o Nickel column purification of anti-FKBP45 peptide antibodies using anti-His resin o Immunoprecipitation depleting FKBP45 peptide from Bm5 nuclear extract with generated anti-FKBP45 peptide antibodies o Epitope mapping of antigenicity in the U1 snRNP using 3D modeling o Predict a framework in which peptides will be selected using a generated 3D molecular model of the U1 snRNP o Use of the programs BioEdit, I-TASSER, Pymol, ExPASy to select a set of epitopes from the fourteen U1 snRNP proteins with potential antigenicity due to placement within the complex and other physiochemical characteristics
ELISA testing of autoimmune activity of 8 synthesized peptides using SLE patient sera Development of a Quality Control protocol for testing serum Optimizing dilution curves for the most and least reactive SLE sera and healthy sera In-silico assembly of spliceosomal polypeptides present in the silk moth Bombyx mori o manuscript in preparation for submission o BLAST search of protein and RNA sequences in seven evolutionarily related insects to Bombyx mori o BioEdit sequence alignment in order to compare assembled sequences and perform data analysis: Identifying conserved domains present in each polypeptide family Calculation of % Identity of Bombyx mori protein similarity to other insect proteins and similarity between insects Analysis of Variance on % Identity values – 08/2007 Volunteer Mercy Hospital, Histopathology Laboratory Miami, Fl • Dr. R Spencer Howell, MD, Pathologist; phone # 305-6630069 • Volunteer Resources, 305-285-2773 Pathology specimen delivery, accessioning, and labeling Inventory stocking Assisting the technologists and the pathologists o
o
06/2007
o o o
SPECIAL COMPETENCIES AND TECHNIQUES Molecular Biology: PCR, 2D Electrophoresis, agarose gel electrophoresis, SDS-Polyacrylamide gel electrophoresis, Bradford protein concentration assay, protein dialysis, nickel affinity column chromatography, assisting with in-vitro transcription and in-vitro splicing experiments, authorized user of radioactive materials. Cell Biology: Maintenance of three stable cell lines (Bm5, SF9 and HeLa), protein nuclear material extraction from Bm5 cell line. CBC using hemocytometer, MD-16 Coulter blood sample reader, recognizing normal and abnormal blood cells in bone marrow and peripheral blood smears. o Wright’s staining, HE staining, Leukocyte Alkaline Phosphatase staining Immunology: Western transfer and blotting, ELISA, immunoprecipitation, performed various enzyme immunoassays for the detection of Syphilis, Rubella, RF, Mononucleosis, hcG, CRP. Microbiology:
Preparation of broth and agar plates for bacterial culture, antibody extraction from E.coli cells, Tease Mount, Scotch Tape, Microslide culture preparation for mycological laboratory diagnosis, Sedimentation and Flotation preparations for parasitic analysis. o Gram Stain, Giemsa stain, Acid fast staining, India ink staning Bioinformatics: BLAST, PyMOL 3D modeling, I-TASSER, BioEdit, ExPASy, Pepitope and Bepipred antigenic epitope prediction programs. Other: Phlebotomy experience, urine, CSF, fecal and semen analysis. Clinical Laboratory Trainee License No. TR41157, State of Florida Department of Health ACTIVITIES Secretary, Student Government Association, Miami Dade College Medical Campus, Miami, Fl August 2007- May 2008 o Serve the student body of Miami Dade College by fighting for student issues, legislative issues and putting on events for the students together with Student Life. o
Received Student Service Grant for volunteering over 300 hours to SGA.
Secretary, Phi Sigma Pi National Honor Fraternity, UCF, Orlando, Fl Fall 2004- Spring 2007 o Participated in feeding the homeless, Teach for America and the Relay for Life American Cancer Society Team in addition to many other Fraternity service, fundraising and scholarship events. Volunteer UCF, Alternative Spring Break, Rebuilding New Orleans, New Orleans, LA Spring Break 2007 o Assisted New Orleans residents in renewing and rebuilding their homes that were devastated by hurricane Katrina. LANGUAGES Fluent in both Spanish and English
FERRER
EDUCATION 08/2003 – 05/2007 B.S., University of Central Florida Orlando, Fl Major: Liberal Studies, Health and Physical Sciences; Minor: Biology 08/2007 – 05/2009 M.S., Barry University Shores, Fl Major: Biomedical Sciences 08/2007 – 05/2008 MT, Miami Dade College Major: Medical Laboratory Technology
Miami
Miami, Fl
RELATED WORK EXPERIENCE 03/2008 – Present Laboratory Research Intern Florida International University, School of Medicine, Molecular Biology Laboratory Miami, Fl • PI: Dr. Rene J. Herrera, PhD; phone # (305) 348-1258 • Doctoral Fellow: Jason A. Somarelli o Using antibodies, generated through in vitro phage display technology, for the recognition of an FKBP45 peptide. o Extraction of antibodies from E.coli in order to express, isolate and purify the anti-FKBP45 peptide antibodies from phage display clones o ELISA detection to test the affinity of anti-FKBP45 peptide antibodies o Western blot detection to test the specificity of anti-FKBP45 peptide antibodies o Nickel column purification of anti-FKBP45 peptide antibodies using anti-His resin o Immunoprecipitation depleting FKBP45 peptide from Bm5 nuclear extract with generated anti-FKBP45 peptide antibodies o Epitope mapping of antigenicity in the U1 snRNP using 3D modeling o Predict a framework in which peptides will be selected using a generated 3D molecular model of the U1 snRNP o Use of the programs BioEdit, I-TASSER, Pymol, ExPASy to select a set of epitopes from the fourteen U1 snRNP proteins with potential antigenicity due to placement within the complex and other physiochemical characteristics
ELISA testing of autoimmune activity of 8 synthesized peptides using SLE patient sera Development of a Quality Control protocol for testing serum Optimizing dilution curves for the most and least reactive SLE sera and healthy sera In-silico assembly of spliceosomal polypeptides present in the silk moth Bombyx mori o manuscript in preparation for submission o BLAST search of protein and RNA sequences in seven evolutionarily related insects to Bombyx mori o BioEdit sequence alignment in order to compare assembled sequences and perform data analysis: Identifying conserved domains present in each polypeptide family Calculation of % Identity of Bombyx mori protein similarity to other insect proteins and similarity between insects Analysis of Variance on % Identity values – 08/2007 Volunteer Mercy Hospital, Histopathology Laboratory Miami, Fl • Dr. R Spencer Howell, MD, Pathologist; phone # 305-6630069 • Volunteer Resources, 305-285-2773 Pathology specimen delivery, accessioning, and labeling Inventory stocking Assisting the technologists and the pathologists o
o
06/2007
o o o
SPECIAL COMPETENCIES AND TECHNIQUES Molecular Biology: PCR, 2D Electrophoresis, agarose gel electrophoresis, SDS-Polyacrylamide gel electrophoresis, Bradford protein concentration assay, protein dialysis, nickel affinity column chromatography, assisting with in-vitro transcription and in-vitro splicing experiments, authorized user of radioactive materials. Cell Biology: Maintenance of three stable cell lines (Bm5, SF9 and HeLa), protein nuclear material extraction from Bm5 cell line. CBC using hemocytometer, MD-16 Coulter blood sample reader, recognizing normal and abnormal blood cells in bone marrow and peripheral blood smears. o Wright’s staining, HE staining, Leukocyte Alkaline Phosphatase staining Immunology: Western transfer and blotting, ELISA, immunoprecipitation, performed various enzyme immunoassays for the detection of Syphilis, Rubella, RF, Mononucleosis, hcG, CRP. Microbiology:
Preparation of broth and agar plates for bacterial culture, antibody extraction from E.coli cells, Tease Mount, Scotch Tape, Microslide culture preparation for mycological laboratory diagnosis, Sedimentation and Flotation preparations for parasitic analysis. o Gram Stain, Giemsa stain, Acid fast staining, India ink staning Bioinformatics: BLAST, PyMOL 3D modeling, I-TASSER, BioEdit, ExPASy, Pepitope and Bepipred antigenic epitope prediction programs. Other: Phlebotomy experience, urine, CSF, fecal and semen analysis. Clinical Laboratory Trainee License No. TR41157, State of Florida Department of Health ACTIVITIES Secretary, Student Government Association, Miami Dade College Medical Campus, Miami, Fl August 2007- May 2008 o Serve the student body of Miami Dade College by fighting for student issues, legislative issues and putting on events for the students together with Student Life. o
Received Student Service Grant for volunteering over 300 hours to SGA.
Secretary, Phi Sigma Pi National Honor Fraternity, UCF, Orlando, Fl Fall 2004- Spring 2007 o Participated in feeding the homeless, Teach for America and the Relay for Life American Cancer Society Team in addition to many other Fraternity service, fundraising and scholarship events. Volunteer UCF, Alternative Spring Break, Rebuilding New Orleans, New Orleans, LA Spring Break 2007 o Assisted New Orleans residents in renewing and rebuilding their homes that were devastated by hurricane Katrina. LANGUAGES Fluent in both Spanish and English
