Microscope

Introduction to the Microscope Care Parts Focusing

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Always carry with 2 hands Only use lens paper for cleaning Do not force knobs Always store covered Keep objects clear of desk and cords

Eyepiece

Revolving lens adjustment Objective Lens Stage Clips

Light

Arm Stage Coarse Focus Fine Focus Base

• Place the Slide on the microscope, specimen side up • Plug into outlet • Use Stage Clips • Click objective lens to the lowest (shortest) setting. • Look into the Eyepiece • Use the Coarse Focus

• Follow steps to focus using low power • Click the lens to the longest objective • Do NOT use the Coarse Focusing Knob – That’s the big one

• Use the Fine Focus Knob until you can see specimen clearly – That’s the small one

Making a “Wet Mount” slide •

Gather a thin slice/piece of your specimen. If your specimen is too thick, then the coverslip will wobble on top of the sample like a see-saw: 1.

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Making a “Wet Mount” slide • 2. Place ONE drop of water directly over the specimen.

Making a “Wet Mount” slide • 3. Place

the coverslip at a 45 degree angle (approximately), with one edge touching the water drop, and let go.

Making a “Wet Mount” slide • Dropping the cover slip directly onto the slide will cause air bubbles to be trapped