Methods In Molecular Biology - Rial Library - Methods And Protocol

methods in molecular biology combinatorial library methods and protocols pdf, 3.9 mb, 367 pages contents 1 using a noncovalent protection strategy to enhance solid-phase synthesis 1. introduction 1.1. noncovalent protection in solid-phase peptide synthesis 1.2. noncovalent protection in solid-phase rhodamine-labeled peptide nucleic acid synthesis 2. materials 2.1. preparation of 18-crown-6 ether complexes of peptides and amino acids 2.2. preparation of fluorescein-labeled pnas on a solid support 3. methods 3.1. preparation of amino acid and peptide complexes with 18-crown-6 (see note 4) 3.2. solid-phase synthesis of nh2-phe-gly-gly-pro-asp-leu-tyr-oh heptapeptide by the fragment condensation approach using noncovalent protection of dipeptide glycylglycine 3.3. solid-phase synthesis of rhodamine labeled peptide nucleic acids using noncovalent protection 4. notes 2 quality control of solid-phase synthesis by mass spectrometry 1. introduction 2. materials 2.1. solid-phase peptide synthesis 2.2. mass spectrometry instrumentation 3. methods 3.1 sample conditioning 3.2. acquisition of a s-sims spectrum 3.3. acquisition of a s-sims image 3 preparation of encoded combinatorial libraries for drug discovery 1. introduction 2. materials 2.1. library design 2.2. library synthesis 2.3. library screening 2.4. compound decoding 2.5. sar data analysis 3. methods 3.1. library design 3.2. library synthesis and encoding 4 simple tools for manual parallel solid phase synthesis 5 cleavage of compounds from solid phase by gaseous reagents 6 synthesis of dna-binding polyamides 7 the preparation of phenyl-stilbene derivatives using the safety catch linker 8 host�guest chemistry 9 automated structure verification of small molecules libraries using 1d and 2d nmr techniques 10 rapid liquid-phase combinatorial synthesis of heterocyclic libraries 11 soluble polymer-supported methods for combinatorial and organic synthesis 12 analytical methods for optimization and quality control of combinatorial synthesis

13 resolving racemic mixtures using parallel combinatorial libraries 14 ligand libraries for the extraction of metal ions dynamic combinatorial and high-throughput screening methods 15 automated liquid�liquid extraction and ion-exchange solid-phase extraction for initial purification 16 on-bead and solution screening approaches for genomically derived targets 17 design of virtual combinatorial libraries 18 approaches to library design for combinatorial chemistry 19 reagent-based and product-based computational strategies in library design 20 designing combinatorial libraries for efficient screening 21 application of neural networks to large dataset qsar, virtual screening, and library design download : http://rapidshare.de/files/35566274/bellavance-comblib.pdf