D ATA S H E E T
Method Development Column Kits for HPLC and HPLC/MS
Varian’s HPLC method development column kits are specifically designed to allow timely development of rugged and reproducible HPLC and HPLC/MS methods. Each kit incorporates multiple unique selectivities from Varian’s Pursuit™, Pursuit™ XRs and Polaris™ column lines.
Hydrophobic Pursuit XRs C18
Pursuit C18
Polaris C18
Pursuit XRs C8 Pursuit C8 Polaris C8 Pursuit XRs Diphenyl Pursuit Diphenyl
Hydrogen Bonding/ Dipole-Dipole
Pursuit PFP pi-pi
Key Benefits Save time. Quickly find the column that works best for your application.
Get consistent results. Get outstanding reproducibility, column to column and batch to batch.
Choose the right column. Varian’s unique chemistries deliver the selectivity you need for any application.
Replace columns less often. Be confident that your Varian HPLC column will deliver reliable performance for a long time to come.
Find the best method. When used in conjunction with Varian’s Fusion AE™ automated HPLC method development software, users can find the best possible method for separating their selected analytes in the shortest time.
Scale up conveniently. Easily switch from analytical to prep without worry.
Method Development Column Kits for HPLC and HPLC/MS Fusion AE™ Automated Method Development and Validation Software Fusion AE saves you the time and frustration associated with manually modifying one parameter at a time to develop your methods. Used in combination with the HPLC method development column kits, it allows users to quickly determine the optimum column and parameters for any separation. It reduces method development time from weeks to a few days and delivers robust methods that can also be automatically validated.
Fusion AE delivers: • Reduced operator bench-time and method development costs. • Increased sample throughput using the best possible methods. • The confidence of knowing your methods can be automatically validated. • Automated data analysis and reporting to pharmacopoeia and regulatory requirements. To learn more about Fusion AE visit www.varianinc.com.
Varian HPLC Method Development Column Kits Each kit contains a selection of 5 Pursuit™, Pursuit™ XRs, or Polaris™ columns based on the type of analysis you need. Choose from: Polar
Non-Polar
Mass Spec
All-Purpose
Contains four polar-modified stationary phases with unique selectivities for polar compounds. C18 included as a benchmark.
Contains five stationary phases that offer both high throughput and high resolution for hydrophobic compounds.
Specially designed for LC/MS applications with unique selectivities
Contains a variety of selectivities for early stage development
Ordering information General Purpose Method Development Kit #1 Phase Particle Size (microns) Pursuit XRs C18 3 Pursuit XRs Diphenyl 3 Pursuit PFP 3 Polaris C18-A 3 Polaris C18 Ether 3
Length (mm) 150 150 150 150 150
ID (mm) 4.6 4.6 4.6 4.6 4.6
Kit Part Number
A2801150M046
General Purpose Method Development Kit #2 Phase
Particle Size (microns)
Length (mm)
ID (mm)
Kit Part Number
Pursuit XRs C18 Pursuit XRs Diphenyl Pursuit PFP Polaris C18-A Polaris C18 Ether
5 5 5 5 5
150 150 150 150 150
4.6 4.6 4.6 4.6 4.6
A2800150M046
Non-Polar Method Development Kit #3 Phase Particle Size (microns) Pursuit XRs C18 3 Pursuit XRs Diphenyl 3 Pursuit XRs C8 3 Pursuit C18 3 Pursuit Diphenyl 3
Length (mm) 150 150 150 150 150
ID (mm) 4.6 4.6 4.6 4.6 4.6
Kit Part Number
Non-Polar Method Development Kit #4 Phase Particle Size (microns) Pursuit XRs C18 5 Pursuit XRs Diphenyl 5 Pursuit XRs C8 5 Pursuit C18 5 Pursuit Diphenyl 5
Length (mm) 150 150 150 150 150
ID (mm) 4.6 4.6 4.6 4.6 4.6
Kit Part Number
A2811150M046
A2810150M046
Method Development Column Kits for HPLC and HPLC/MS Polar Method Development Kit #5 Phase Particle Size (microns) Pursuit XRs C18 3 Pursuit PFP 3µm 3 Polaris C18-A 3µm 3 Polaris C18 Ether 3µm 3 Polaris Amide-C18 3µm 3
Length (mm) 150 150 150 150 150
ID (mm) 4.6 4.6 4.6 4.6 4.6
Kit Part Number
Polar Method Development Kit #6 Phase Particle Size (microns) Pursuit XRs C18 5µm 5 Pursuit PFP 5 Polaris C18-A 5 Polaris C18 Ether 5 Polaris Amide-C18 5
Length (mm) 150 150 150 150 150
ID (mm) 4.6 4.6 4.6 4.6 4.6
Kit Part Number
LC/MS Method Development Kit#7 Phase Particle Size (microns) Pursuit XRs C18 3 Pursuit XRs Diphenyl 3 Pursuit XRs C8 3 Polaris C18-A 3 Polaris C18 Ether 3
Length (mm) 50 50 50 50 50
ID (mm) 2 2 2 2 2
Kit Part Number
A2821150M046
A2820150M046
A28301050M020
Varian, Inc. www.varianinc.com North America: 800.926.3000, 925.939.2400 Europe The Netherlands: 31.118.67.1000 Asia Pacific Australia: 613.9560.7133 Latin America Brazil: 55.11.3845.0444 Other sales offices and dealers throughout the world— check our Web site GC • LC • MS • GPC/SEC • AA • ICP • ICP-MS • UV-Vis-NIR • FT-IR • Fluorescence • Dissolution • NMR • MRI • FTMS • Consumables • Data Systems Pursuit, Pursuit XRs, Polaris, Varian and the Varian logo are trademarks or registered trademarks of Varian, Inc. in the U.S. and other countries. © 2007 Varian, Inc.
SI-1089 11/07 Printed in U.S.