Lab 6 Transformation Pre Lab

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Pre-Lab: Transformation

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1 . Who accidentally discovered transformation, and what was he attempting? 2. Who is credited with later identifying the transforming factor as DNA? 3. Write a one sentence description of transformation in nature or in the lab. 4. What advanta g e does natural transformation aive a bacterial cell? 5. what are two other means of natural genetic recombination in bacteria? 6. What is the purpose/application of transforming bacteria in the lab? 7. What common drug is produced this way in vast amounts? 8. Assuming the "gene of interest" has been found, how is it obtained? 9. Once obtained, where is it put so that transformation can be attempted? 10. What term applies to the role of the plasmid in this lab procedure? 11. How are bacterial cells such as E.

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made "competent" or receptive?

12. What two genes are we attempting to add to bacteria in our lab? 13. What treatment in our protocol will help the cells to take up the plasmid? 1 4. What favorite snack will help the bacteria recover from the procedure? 15. Why will we take some cells through the procedure without adding plasmid? 1 6. What do "+" and " -" refer to in our protocol and on our tubes? 1 7. Why will we plate out "+" and "-" cells on plain LB agar? 18. Our "-" cells are arnpicillin sensitive. Which plate(s) will provide evidence of that? 19. Why won't our transformed cells grow blue colonies on Lbamp agar? 20. Describe how we will know whether we have been successful in inserting these genes. 21. Your transformation attempt may not be 100% efficient. Explain and predict results that support your answer. Can you imagine how you might estimate efficiency?