Instructions For Elution Of Plasmids From Whatman Paper (2008)
This document was uploaded by user and they confirmed that they have the permission to share it. If you are author or own the copyright of this book, please report to us by using this DMCA report form. Report DMCA
Overview
Download & View Instructions For Elution Of Plasmids From Whatman Paper (2008) as PDF for free.
More details
- Words: 132
- Pages: 2
Instructions for Elution of Plasmids from Whatman Paper a.
The plasmid DNA has been spotted on the filter paper you received, in the RED region defined by the dotted lines, as the diagram has shown (below). 3 cm
3 cm
3 cm 1cm
Spot Plasmid Here Side view of folded paper b.
Cut out the dotted area of the filter paper.
c.
Put 50ul of TE or Elution Buffer together with filter paper into a 1.5ml tube.
d.
Squash paper in tube with pipette tip and vortex to ensure that plasmid is eluted out of filter paper.
e.
Spin down and transfer eluted plasmid present in supernatant into another tube.
f.
Plasmid is now ready for transformation and bacterial propagation using Ampicillin for selection. We routinely use TOP10 cells (Invitrogen) for electroporation.
The plasmid DNA has been spotted on the filter paper you received, in the RED region defined by the dotted lines, as the diagram has shown (below). 3 cm
3 cm
3 cm 1cm
Spot Plasmid Here Side view of folded paper b.
Cut out the dotted area of the filter paper.
c.
Put 50ul of TE or Elution Buffer together with filter paper into a 1.5ml tube.
d.
Squash paper in tube with pipette tip and vortex to ensure that plasmid is eluted out of filter paper.
e.
Spin down and transfer eluted plasmid present in supernatant into another tube.
f.
Plasmid is now ready for transformation and bacterial propagation using Ampicillin for selection. We routinely use TOP10 cells (Invitrogen) for electroporation.
Related Documents
Plasmids
May 2020 3
Instructions For Paper Presentation- M
October 2019 12
Instructions From The Cook
May 2020 4
Aletha Paper 1 Instructions
October 2019 10