Instructions For Elution Of Plasmids From Whatman Paper (2008)

  • June 2020
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Instructions for Elution of Plasmids from Whatman Paper a.

The plasmid DNA has been spotted on the filter paper you received, in the RED region defined by the dotted lines, as the diagram has shown (below). 3 cm

3 cm

3 cm 1cm

Spot Plasmid Here Side view of folded paper b.

Cut out the dotted area of the filter paper.

c.

Put 50ul of TE or Elution Buffer together with filter paper into a 1.5ml tube.

d.

Squash paper in tube with pipette tip and vortex to ensure that plasmid is eluted out of filter paper.

e.

Spin down and transfer eluted plasmid present in supernatant into another tube.

f.

Plasmid is now ready for transformation and bacterial propagation using Ampicillin for selection. We routinely use TOP10 cells (Invitrogen) for electroporation.