Hts- Protocol Tips From Papers

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High magnification- high resolution magnification-low resolution image.

image

VS

low

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ADMET-Administration, and Toxicity

Distribution,

Metabolism,

Excretion

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Micro nuclei compound

detection

for

assaying

genotoxicity

of

a

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Validate the suitability of biomarkers of toxicity and probes to quantify the change to achieve a mechanistic understanding of the biological perturbations caused due to nanomaterial interaction with cells.

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Systems toxicology describe the toxicogenomics evaluation of biological systems, involving perturbation by toxicants and stressors, monitoring molecular expression and conventional toxicological parameters, and iteratively integrating response data to model the toxicological

How to identify the morphology change using automated imaging techniques (Cell morphology- drug discovery (PLOS Biology)) 

Cellular and organelle morphology changes can be measured from segmented images of cells stained with DNA and microtubule markers using algorithms that identify cell and nuclear boundaries.

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Combining segmentation and intensity distribution algorithms allows acquisition of multiple shape-, texture-, and intensity-related features for each image collected.

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For each object identified by the segmentation algorithms, collected attributes include object location, area, perimeter, and axis ratio, as well as pixel-intensity sum, mean, variance, and kurtosis (the degree of peakedness of a distribution).

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