Hahn Et Al Intext3
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Intext 3
Step Reverse Transcription PCR Initial activation step
Time 30 min
Temperature 50°C
15 min
95°C
3-step cycling Denaturation Annealing Extension
15 s 30 s 30 s
94°C 55°C 72°C
Cycle number
40 cycles
Additional Comments
HotStarTaq® DNA Polymerase is activated by this heating step.
Perform fluorescence data collection
Step Reverse Transcription PCR Initial activation step
Time 30 min
Temperature 50°C
15 min
95°C
3-step cycling Denaturation Annealing Extension
15 s 30 s 30 s
94°C 55°C 72°C
Cycle number
40 cycles
Additional Comments
HotStarTaq® DNA Polymerase is activated by this heating step.
Perform fluorescence data collection
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