Gfp Antibody

Novus Biologicals, Inc PO Box 802 Littleton, CO 80160 Phone:1-888-506-6887 Fax: 303-730-1966 Email: [email protected] Go to: www.NovusBio.com

Rabbit Polyclonal anti-GFP Catalog Number:

NB600-303

Background:

A highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin. In immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells. It is routinely used in immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP-immunohistochemistry (1:200) on whole-mount mouse embryos.

Alternate Names:

anti-Green Fluorescent Protein antibody, anti-EGFP antibody, anti-YFP antibody

Host:

Rabbit

Research Areas:

Epitope Tags and Markers,GFP Antibodies,IHC Grade

Immunogen:

Highly purified recombinant full length protein made in E. coli. The antibody is directed against the entire GFP molecule.

Isotype:

IgG

Species Reactivity:

Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.

Uses:

This antibody is useful for microscopy, immunocytochemistry, immunohistochemistry (paraffin-embedded sections), and immunoprecipitation**. *IHC-P: Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol (13min in 10mM citrate buffer pH6 followed by rapid cooling on ice). **IP: Use 1 ul crude serum per 10cm tissue culture dish of transfected cells. Use 10ul protein A agarose CL4B (Amersham-Pharmacia) to precipitate immune complex. WB: Use in 1x BLOTTO or 3% BSA in PBS. Optimal dilutions/concentrations should be determined by the end user. * Other applications have not been tested.

Dilutions:

Suggested working dilutions * immunoprecipitation **, Western Blot 1:2,500 - 1:10,000, Immunocytochemistry (Use in 4% normal serum) 1:200 - 1/1000, Immunohistochemistry-Paraffin 1:200 *, Immunogold Electron Microscopy 1:100 * Investigator should determine optimal working dilutions.

Positive Controls:

Detects 5ng of recombinant GFP (using ECL or ECL Plus) in under one minute of exposure to film.

Packaging:

0.05 ml Whole Rabbit antisera.

Concentration:

5.9 mg/ml

Buffer:

0.02M KPO4, 0.15M NaCl [pH 7.2]

(c) 2006 Novus Biologicals - GFP anti- - Page 1

Novus Biologicals, Inc PO Box 802 Littleton, CO 80160 Phone:1-888-506-6887 Fax: 303-730-1966 Email: [email protected] Go to: www.NovusBio.com

Preservative:

0.01% sodium azide

Storage:

Store at 4 degrees C for up to 2 weeks. Aliquot and store at -20 degrees C or -80 degrees C for long-term storage. Avoid repeated freeze/thawing.

Notes:

NB 600-308 is the purified version of this antibody.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

Product Specific References: 1. Zhang, Z., Shao, S., and Meistrich, M. L. Irradiated Mouse Testes Efficiently Support Spermatogenesis Derived From Donor Germ Cells of Mice and Rats. Biol. of Reprod. 27: 365-375, 2006. Novus Specific References:

1. Day, Y, Huang, et al. Renal protection from ischemia mediated by A2A adenosine receptors on bone marrow-derived cells. J. Clin. Invest. 112(6): 883-891, 2003. 2. Loewen, N., et al. Long-Term, Targeted Genetic Modification of the Aqueous Humor Outflow Tract Coupled with Noninvasive Imaging of Gene Expression In Vivo. Investigative Opthalmology & Visual Science. 45: 3091-3098, 2004. 3. Day, Y-J., et al. A2a adenosine receptors on bone marrow-derived cells protect liver from ishemia-reperfusion injury. J. Immunology. 174:5040-5046, 2005. [Immunohistochemistry-paraffin, Mouse] 4. Jodele, S., et al. The Contribution of Bone Marrow-Derived Cells to the Tumor Vasculature in Neuroblastoma is Matrix Metalloproteinase-9 Dependent. Cancer Res. 65(8): 3200-3208, 2005. 5. Zhang, Z., et al. Irradiated mouse testes efficiently support sprematogenesis derived from donor germ cells of mice and rats. J. Androl. 27: 265-275, 2006.

Image(s)

(c) 2006 Novus Biologicals - GFP anti- - Page 2

Novus Biologicals, Inc PO Box 802 Littleton, CO 80160 Phone:1-888-506-6887 Fax: 303-730-1966 Email: [email protected] Go to: www.NovusBio.com

Recombinant GFP detected using NB 600-303. 10 second exposure.

(c) 2006 Novus Biologicals - GFP anti- - Page 3