General Stem Cell Biology Brochure

  • December 2019
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General Stem Cell Biology

n Stem Cell Resources n Stem Cell Pathways n Stem Cell Markers

Infinite Expansion....Infinite Potential

n Stem Bio Overview n Protocols

Stem Cell Biology General Stem Cell Biology

Infinite Expansion....Infinite Potential Stem cells hold the key to a number of cellular processes from development, tissue regeneration, and aging. They also hold the promise of cures for many diseases and injuries as well as offer an opportunity to bridge the ‘animal to human gap’ in ADMET and drug efficacy studies, a necessary step in providing therapeutic products for human use. The potential of stem cells is certain, however bringing this potential to fruitition is contingent upon several key factors, not the least of them being the highly motivated and inteligent global research

Isolation

Characterization

Expansion

community. These scientists have and continue to contribute key incremental discoveries on nearly a daily basis and these discoveries move the entire science closer and closer to becoming the numerous miracle therapies that we have always been predicted. The stem cell biology platform, as presented in this brochure, highlights the most common general use media, supplements, reagents, and tools for stem cell discovery culture and analysis. Additional products can be found throughout the Sigma-Aldrich® catalog and website.

Differentiation

In vivo Tracking

Functional Profiling

Stem Cell Fate Germ Cells

Bone Marrow Blood

Mesoderm Bone Inner Cell Mass

Muscle Lung

ESC’s

Liver Intestine Endoderm Pancreas Stomach Esophagus Skin Ectoderm Neural Tissue

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Origins and Differentiation of Tissues Nervous tissue/NSCs Ectoderm Skin/skin SCs

Muscle and bone/tissue SC Lung, liver, and pancreas/organ specific SCs

Gastrulation

1

Endoderm

Esophagus, stomach, and intestine/intestinal SCs

Primordial germ cells

General Stem Cell Biology

Bone marrow and blood/HSCs and MSCs Mesoderm

Nervous tissue Ectoderm Skin Bone marrow and blood Mesoderm Muscle and bone Lung, liver, and pancreas

Gastrulation

2

Endoderm

Esophagus, stomach, and intestine

Primordial germ cells NSCs/skin SCs HSCs/MSCs/tissue SCs

Multipotent stem cells

Organ SCs/intestinal SCs

Putative developmental ontogeny of stem cells In lineage tree 1, the development of stem cells occurs after the formation of germ layers. These stem cells are thus restricted by germ layer commitment to their respective lineage (e.g., mesoderm is formed, giving rise to hematopoietic progenitors that become hematopoietic stem cells). Lineage tree 2 illustrates

the area that stem cells might develop similarly to PGCs, in that they avoid the lineage commitments during gastrulation and subsequently migrate to specific tissue and organ niches. (adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)

Our Innovation, Your Research



Shaping the Future of Life Science

3

Genomic reprogramming in the oocyte, early embryos, and the germ line

General Stem Cell Biology

Specification of germ cells

Founder PGC (E7.5)

STELLA OCT-4 ESG-1 NANOG SOX2

ES cells

EG cells

Blastocyst (E7.5) Maternal inheritance & regulation of pluripotency EZH2 EED HP1

Migrating PGCs

DNA demethylation X re-activation erasure of imprints

STELLA OCT-4 ESG-1 Oocyte Initiation of imprinting

Epigenetic reprogramming Genital ridge E11.5

(adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)

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Over 500 immunohistochemical images for every antibody



Publicly available data on the web

Look even closer. Go to sigma.com/prestige for more information. Our innovation, Your Research – Shaping the Future of Life Science Prestige Antibodies is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

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Product List

Workflow

Product Class

Isolation

Cryopreservation

C6164

Cell Freezing Medium-DMSO 1×

Isolation

Cryopreservation

C6295

Cell Freezing Medium-DMSO Serum free 1x

Isolation

Cryopreservation

C6039

Cell Freezing Medium-Glycerol 1×

Isolation

Cryopreservation

C2639

Cell Freezing Medium-Serum-free 1×

Isolation

Cryopreservation

D2650

Dimethyl sulfoxide

Isolation

Dissociation

E8008

EDTA solution

Isolation

Dissociation

E8140

Elastase from human leukocytes

Isolation

Enzymes

C2014

Collagenase, rat islet isolation

Isolation

Enzymes

C6079

Collagenase + Protease Inhibitors, suitable for islet isolation

Isolation

Enzymes

C9572

Collagenase, g-irradiated

Isolation

Enzymes

C9722

Collagenase, sterile, cell culture

Isolation

Enzymes

D4818

Dispase I

Isolation

Enzymes

D4693

Dispase II

Isolation

Isolation

a0561

ACCUSPIN™ System-Histopaque®-1077 Box of 100 tubes. Each 50 ml tube contains 15 ml Histopaque 1077-1 and will separate between 15 to 30 ml of anticoagulated blood.

Isolation

Isolation

A6929

ACCUSPIN System-Histopaque-1077 Box of 40 tubes. Each 12 ml tube contains 3 ml of Histopaque 1077-1 and will separate between 3 to 6 ml of anticoagulated blood.

Isolation

Isolation

A7054

ACCUSPIN System-Histopaque-1077 Box of 12 tubes. Each 50 ml tube contains 15 ml of Histopaque 1077-1 and will separate between 15 to 30 ml of anticoagulated blood.

Isolation

Isolation

F1418

Ficoll® 400 SigmaUltra, Type 400-DL, lyophilized powder

Isolation

Isolation

F2878

Ficoll PM 70 Type 70

Isolation

Isolation

F4375

Ficoll PM 400 Type 400, lyophilized powder

Isolation

Isolation

F5415

Ficoll solution Type 400, 20% in H2O

Isolation

Isolation

F8016

Ficoll 400 Type 400-DL, lyophilized powder, cell culture tested

Isolation

Isolation

F8636

Ficoll 400 lyophilized powder, γ-irradiated, cell culture tested

Isolation

Isolation

F9378

Ficoll 400 Type 400-DL, lyophilized powder

Isolation

Isolation

H8889

Histopaque-1077 Hybri-Max™ liquid, sterile-filtered, hybridoma tested

Isolation

Reagents

C6164

Cell Freezing Medium-DMSO

Characterization

Antibodies

A2726

Anti-ADAM-10, Cytoplasmic Domain antibody produced in rabbit

Characterization

Antibodies

A2851

Anti-ADAM-10, Prohormone Convertase Activation Site antibody produced in rabbit

Characterization

Antibodies

A2976

Anti-ADAM-12, N-terminal antibody produced in rabbit

Characterization

Antibodies

A3101

Anti-ADAM-10, Propeptide Domain antibody produced in rabbit

Characterization

Antibodies

A3226

Anti-ADAM-10, N-Terminal antibody produced in rabbit

Characterization

Antibodies

A3976

Anti-ADAM-17, Activation Site antibody produced in rabbit

Characterization

Antibodies

A4226

Anti-ADAM-17, Propeptide Domain antibody produced in rabbit

Characterization

Antibodies

A4351

Anti-ADAM-17, Cytoplasmic Domain antibody produced in rabbit

Characterization

Antibodies

A5102

Anti-Aurora B antibody produced in rabbit

Isolation

Product Number

Characterization

Product Name

Expansion

Our Innovation, Your Research

Differentiation



Functional Profiling

Shaping the Future of Life Science

General Stem Cell Biology

In some instances scientific milestones appear as serendipitous, nearly accidental discoveries. But for most of us discovery is a process and series of small steps that are the result of a long and wide pathway of techniques, methods, and in-depth analysis. In order to serve you better we have listed our the relevant products in the following pages by ‘work flow’ which is essentially addressing all of the steps in your discovery process. For every area of study, including regenerative medicine, there is a step-wise and systematic approach in gathering data. The proceeding products have been listed to appear in order of process: Isolation – products for enzymatic or gradient cell separation as well as storage/cryopreservation Characterization – product for cell identification Expansion – product to increase cell populations Differentiation – products to move cells forward along a given lineage and confirm lineage markers Functional profiling – products to assist in converting or diverting the intercellular processes, mechanisms, and pathways In vitro/in vivo analysis – products for tagging/marking cells in vitro and in vivo

In vivo Tracking

5

General Stem Cell Biology

Product List, cont. Workflow

Product Class

Characterization

Antibodies

A5228

Characterization

Antibodies

A5691

Anti-Smooth muscle alpha-actin - alkaline phosphatase, produced in mouse

Characterization

Antibodies

B3428

Anti-Bax antibody produced in rabbit

Characterization

Antibodies

B8429

Monoclonal Anti-Bax antibody produced in mouse, 2 mg/mL

Characterization

Antibodies

B9553

Monoclonal Anti-Bone Morphogenetic Protein 2

Characterization

Antibodies

C1366

Anti-CXCR3 antibody produced in rabbit

Characterization

Antibodies

C2542

Monoclonal Anti-N-Cadherin antibody produced in mouse

Characterization

Antibodies

C2667

Monoclonal Anti-N-Cadherin antibody produced in mouse

Characterization

Antibodies

C3116

Anti-CXCR4 antibody produced in rabbit

Characterization

Antibodies

C3366

Anti-CXCR5 antibody produced in rabbit

Characterization

Antibodies

C3865

Monoclonal Anti-N-Cadherin antibody produced in mouse

Characterization

Antibodies

C3960

Anti-Ciliary Neurotrophic Factor antibody, human, produced in goat

Characterization

Antibodies

C4085

Anti-Ciliary Neurotrophic Factor antibody, rat, produced in goat

Characterization

Antibodies

C4585

Monoclonal Anti-β-Tubulin−Cy3 antibody produced in mouse

Characterization

Antibodies

C5588

Anti-Cyclin D1 antibody produced in rabbit

Characterization

Antibodies

C6198

Anti-Smooth muscle alpha-actin - Cy3

Characterization

Antibodies

C7930

Anti-CD3, T Cell antibody produced in rabbit whole antiserum

Characterization

Antibodies

C8227

Anti-CXCR4 (Fusin), N-Terminal antibody produced in rabbit

Characterization

Antibodies

C8352

Anti-CXCR4 (Fusin) (182-196) antibody produced in rabbit

Characterization

Antibodies

C8354

Anti-CX3CR-1, N-Terminal antibody produced in rabbit

Characterization

Antibodies

C8479

Anti-CX3CR-1 (175-189) antibody produced in rabbit

Characterization

Antibodies

C9080

Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse

Characterization

Antibodies

D2442

Anti-Dopamine Transporter antibody produced in rabbit

Characterization

Antibodies

D4567

Anti-DNMT1, human, rat, mouse, antibody produced in rabbit

Characterization

Antibodies

D4692

Anti-DNMT1, human, mouse, antibody produced in rabbit

Characterization

Antibodies

D5567

Anti-dimethyl-Histone H3 (diMe-Lys9), hu, mo, rat, bovine, chicken, frog, Drosophila, C. elegans, A. thaliana produced in rabbit

Characterization

Antibodies

D5692

Anti-dimethyl-Histone H3 (diMe-Lys4) hu, mo, rat, bovine, chicken, frog, Drosophila, C. elegans, A. thaliana, produced in rabbit

Characterization

Antibodies

D9442

Anti-Dopamine Transporter, Extracellular Loop 2 antibody produced in rabbit

Characterization

Antibodies

D9567

Anti-Dopamine Transporter, C-Terminus antibody produced in rabbit

Characterization

Antibodies

E160

Anti-Eotaxin antibody produced in goat

Characterization

Antibodies

E1906

Anti-EAPII (TTRAP) antibody produced in rabbit

Characterization

Antibodies

F0300

Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit

Characterization

Antibodies

F0425

Anti-Fibroblast Growth Factor Receptor-3, Cytoplasmic antibody produced in rabbit

Characterization

Antibodies

F0550

Anti-Flt-3 antibody produced in goat

Characterization

Antibodies

F2043

Monoclonal Anti-β-Tubulin−FITC antibody produced in mouse

Characterization

Antibodies

F3304

Anti-Frizzled-2 antibody produced in rabbit

Characterization

Antibodies

F3393

Anti-Fibroblast Growth Factor-Basic (1-24) antibody produced in rabbit

Characterization

Antibodies

F3520

Anti-vonWillebrand Factor produced in rabbit

Characterization

Antibodies

F3648

Anti-Fibronectin antibody produced in rabbit

Characterization

Antibodies

F3777

Anti-Smooth muscle alpha-actin - FITC, produced in mouse

Characterization

Antibodies

F3804

Anti-Frizzled-1 antibody produced in rabbit

Characterization

Antibodies

F3922

Anti-Fibroblast Growth Factor Receptor-3, Extracellular antibody produced in rabbit

Characterization

Antibodies

F5421

Anti-Fibroblast Growth Factor Receptor-1 antibody produced in rabbit

Characterization

Antibodies

F5521

Anti-Fibroblast Growth Factor-Acidic antibody produced in rabbit

Isolation

6

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Characterization

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Product Number

Expansion

Product Name Anti-Smooth muscle actin, produced in mouse

Differentiation

TECHNICAL SERVICE: 800-325-5832

Functional Profiling

In vivo Tracking

Product Class

Characterization

Antibodies

F5537

Anti-Fibroblast Growth Factor-Basic antibody produced in rabbit

Characterization

Antibodies

F5900

Monoclonal Anti-Interleukin-2−FITC antibody produced in mouse, clone 5334.2

Characterization

Antibodies

F6796

Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit

Characterization

Antibodies

F8925

Anti-Flt-3/Flk-2 ligand (FL) antibody, rec mo

Characterization

Antibodies

F9049

Anti-Fibroblast Growth Factor-10 antibody produced in goat

Characterization

Antibodies

F9050

Anti-Flt-3/Flk-2 ligand (FL) antibody produced in goat

Characterization

Antibodies

F9304

Anti-FBI-1/Pokemon antibody produced in rabbit

Characterization

Antibodies

F9429

Anti-FBI-1/Pokemon (C-terminal) antibody produced in rabbit

Characterization

Antibodies

G0157

Anti-GABA Transporter GAT-1 antibody produced in rabbit

Characterization

Antibodies

G0290

Anti-GATA1 antibody produced in rabbit

Characterization

Antibodies

G0907

Anti-Granulocyte-Macrophage Colony Stimulating Factor

Characterization

Antibodies

G1544

Anti-GFP, N-terminal antibody produced in rabbit

Characterization

Antibodies

G4154

Anti-Granulocyte-Macrophage Colony Stimulating Factor Receptor α

Characterization

Antibodies

G4165

Anti-gp130 antibody produced in rabbit

Characterization

Antibodies

G4420

Anti-GRP94 (C-terminal) antibody produced in rabbit

Characterization

Antibodies

G4545

Anti-GRP94 antibody produced in rabbit

Characterization

Antibodies

G7657

Anti-GABA Transporter GAT-2 antibody produced in rabbit

Characterization

Antibodies

G8043

Anti-phospho-Growth Associated Protein-43 (pSer41) antibody produced in rabbit

Characterization

Antibodies

G8044

Anti-GATA-4 (pSer105) antibody produced in rabbit

Characterization

Antibodies

G8407

Anti-GABA Transporter GAT-3 antibody produced in rabbit

Characterization

Antibodies

G8918

Anti-GRP78/BiP (GL-19) antibody produced in rabbit

Characterization

Antibodies

G9043

Anti-GRP78/BiP (ET-21) antibody produced in rabbit

Characterization

Antibodies

G9152

Anti-Galactocerebroside antibody produced in rabbit whole antiserum

Characterization

Antibodies

G9269

Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit

Characterization

Antibodies

H0163

Monoclonal Anti-Histone Deacetylase 4 (HDAC4) antibody produced in mouse

Characterization

Antibodies

H0164

Anti-Histone H3 antibody produced in rabbit

Characterization

Antibodies

H0412

Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit

Characterization

Antibodies

H0788

Monoclonal Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in mouse

Characterization

Antibodies

H0913

Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in mouse

Characterization

Antibodies

H2039

Anti-HP1β antibody produced in rabbit

Characterization

Antibodies

H2164

Anti-HP1α antibody produced in rabbit

Characterization

Antibodies

H2287

Anti-Histone Deacetylase 6 (HDAC6) antibody produced in rabbit

Characterization

Antibodies

H2537

Anti-Histone Deacetylase 7 (HDAC7) (YS-19) antibody produced in rabbit

Characterization

Antibodies

H2662

Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit

Characterization

Antibodies

H2663

Monoclonal Anti-Histone Deacetylase 2 (HDAC2) antibody produced in mouse

Characterization

Antibodies

H2913

Monoclonal Anti-Histone Deacetylase 11 (HDAC11) antibody produced in mouse

Characterization

Antibodies

H3034

Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit

Characterization

Antibodies

H3159

Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit

Characterization

Antibodies

H4538

Monoclonal Anti-Histone Deacetylase 5 (HDAC5) antibody produced in mouse

Characterization

Antibodies

H5912

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit

Characterization

Antibodies

H6287

Monoclonal Anti-Histone Deacetylase 1 (HDAC1) antibody produced in mouse

Characterization

Antibodies

H6409

Monoclonal Anti-phospho-Histone H3 (pSer10) antibody produced in mouse

Characterization

Antibodies

H6412

Monoclonal Anti-Histone Deacetylase 8 (HDAC8) antibody produced in mouse

Characterization

Antibodies

H6537

Monoclonal Anti-Histone Deacetylase 3 (HDAC3) antibody produced in mouse

Characterization

Antibodies

H6663

Monoclonal Anti-Histone Deacetylase 7 (HDAC7) antibody produced in mouse

Isolation

Product Number

Characterization

Product Name

Expansion

Our Innovation, Your Research

Differentiation



Functional Profiling

Shaping the Future of Life Science

General Stem Cell Biology

Workflow

In vivo Tracking

7

General Stem Cell Biology

Product List, cont. Workflow

Product Class

Characterization

Antibodies

H7162

Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit

Characterization

Antibodies

H7664

Anti-phospho-Histone H1.4 (pSer27) antibody produced in rabbit

Characterization

Antibodies

H7789

Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit

Characterization

Antibodies

H8038

Anti-Histone Deacetylase 8 (HDAC8) antibody produced in rabbit

Characterization

Antibodies

H8163

Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit

Characterization

Antibodies

H9286

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit

Characterization

Antibodies

H9411

Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in rabbit

Characterization

Antibodies

H9536

Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in rabbit

Characterization

Antibodies

H9663

Anti-Histone Deacetylase 5 (HDAC5) (PI-16) antibody produced in rabbit

Characterization

Antibodies

H9908

Monoclonal Anti-phospho-Histone H3 (pSer28) antibody produced in rat

Characterization

Antibodies

I0505

Anti-IκBα antibody produced in rabbit

Characterization

Antibodies

I0649

Monoclonal Anti-Interleukin-6 Receptor antibody produced in mouse

Characterization

Antibodies

I1142

Monoclonal Anti-Interferon-γ antibody produced in rat

Characterization

Antibodies

I1404

Anti-Interleukin-1 Receptor Accessory Protein, C-Terminal antibody produced in rabbit

Characterization

Antibodies

I1644

Anti-Interleukin-5 antibody produced in goat

Characterization

Antibodies

I2143

Anti-Interleukin-6 antibody produced in rabbit

Characterization

Antibodies

I2407

Anti-Interleukin-3 Receptor α antibody produced in goat

Characterization

Antibodies

I2519

Monoclonal Anti-Interleukin-8 antibody produced in mouse

Characterization

Antibodies

I2532

Monoclonal Anti-Interleukin-7 antibody produced in mouse

Characterization

Antibodies

I2657

Anti-Interleukin-5 Receptor α antibody produced in goat

Characterization

Antibodies

I2782

Anti-Interleukin-7 antibody produced in goat

Characterization

Antibodies

I2907

Monoclonal Anti-Interleukin-7 Receptor α antibody produced in mouse

Characterization

Antibodies

I2908

Monoclonal Anti-Interferon-γ antibody produced in hamster

Characterization

Antibodies

I3392

Anti-Interleukin-1α antibody produced in goat

Characterization

Antibodies

I3393

Anti-Interleukin-6 antibody produced in rabbit

Characterization

Antibodies

I3642

Monoclonal Anti-Interleukin-1β antibody produced in mouse

Characterization

Antibodies

I3653

Anti-Interferon β antibody produced in goat

Characterization

Antibodies

I3767

Anti-Interleukin-1β antibody produced in goat

Characterization

Antibodies

I3778

Monoclonal Anti-Interleukin-1α antibody produced in mouse

Characterization

Antibodies

I4153

Anti-Interleukin-12 Receptor β1 antibody produced in goat

Characterization

Antibodies

I4902

Anti-Interferon-α/β Receptor 1 antibody produced in goat

Characterization

Antibodies

I5018

Anti-Interleukin-1α antibody produced in rabbit

Characterization

Antibodies

I5027

Anti-Interferon-γ (IFN-γ) antibody produced in goat

Characterization

Antibodies

I5152

Anti-Interferon-γ Receptor I (IFN-γ RI) produced in goat

Characterization

Antibodies

I5270

Anti-Interleukin-11 antibody produced in goat

Characterization

Antibodies

I5395

Anti-Interleukin-12 antibody produced in goat

Characterization

Antibodies

I5527

Monoclonal Anti-Interleukin-1 Soluble Receptor Type I antibody produced in mouse

Characterization

Antibodies

I5652

Monoclonal Anti-Interleukin-2 Soluble Receptor α antibody produced in mouse

Characterization

Antibodies

I5777

Monoclonal Anti-Interleukin-2 Soluble Receptor β antibody produced in mouse

Characterization

Antibodies

I5902

Monoclonal Anti-Interleukin-2 Soluble Receptor γ antibody produced in mouse

Characterization

Antibodies

I6152

Anti-Interleukin-2 Soluble Receptor α antibody produced in goat

Characterization

Antibodies

I6277

Anti-Interleukin-2 Soluble Receptor β antibody produced in goat

Characterization

Antibodies

I6402

Anti-Interleukin-2 Soluble Receptor γ antibody produced in goat

Characterization

Antibodies

I7401

Anti-Interleukin-2 antibody produced in goat

Characterization

Antibodies

I7642

Anti-Interleukin-12 antibody produced in goat

Isolation

8

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Characterization

ORDER: 800-325-3010

Product Number

Expansion

Product Name

Differentiation

TECHNICAL SERVICE: 800-325-5832

Functional Profiling

In vivo Tracking

Product Class

Characterization

Antibodies

I7901

Monoclonal Anti-Interleukin-6 antibody produced in mouse, clone 6708.11

Characterization

Antibodies

I8026

Anti-Interleukin-8 antibody produced in goat

Characterization

Antibodies

I8153

Anti-Interleukin-1 Receptor Accessory Protein antibody produced in rabbit

Characterization

Antibodies

I8273

Anti-Interleukin-2 antibody produced in goat

Characterization

Antibodies

I8398

Anti-Interleukin-2 antibody produced in goat

Characterization

Antibodies

I8638

Monoclonal Anti-Integrin β1 antibody produced in mouse

Characterization

Antibodies

I9016

Anti-Interferon-γ antibody produced in goat

Characterization

Antibodies

I9141

Anti-Interferon-γ (IFN-γ) antibody produced in goat

Characterization

Antibodies

I9898

Anti-Interleukin-5 antibody produced in goat

Characterization

Antibodies

IMMH2

Characterization

Antibodies

J3877

Anti-JAK3 antibody produced in rabbit

Characterization

Antibodies

J4127

Anti-Jagged 1 antibody produced in goat

Characterization

Antibodies

K4763

Anti-Ku70 antibody produced in rabbit

Characterization

Antibodies

K4888

Anti-Ku80 (XRCC5) (557-570) produced in rabbit

Characterization

Antibodies

L4418

Anti-LSD1 (AOF2) (C-terminal) antibody produced in rabbit

Characterization

Antibodies

L4793

Anti-LSD1 (AOF2) (N-terminal) antibody produced in rabbit

Characterization

Antibodies

L9152

Anti-Leukemia Inhibitory Factor antibody produced in goat

Characterization

Antibodies

L9277

Anti-Leukemia Inhibitory Factor antibody produced in goat

Characterization

Antibodies

M2420

Monoclonal Anti-Monocyte Chemotactic Protein-1 produced in mouse

Characterization

Antibodies

M254

Anti-Monocyte Chemotactic Protein 2 antibody produced in goat

Characterization

Antibodies

M255

Anti-Monocyte Chemotactic Protein 3 produced in goat

Characterization

Antibodies

M257

Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu

Characterization

Antibodies

M257

Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu.

Characterization

Antibodies

M4819

Anti-methyl-Histone H3 (Me-Lys4) antibody produced in rabbit

Characterization

Antibodies

M5917

Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. mo.

Characterization

Antibodies

M6042

Anti-Macrophage Inflammatory Protein-1α antibody produced in goat, rec. hu.

Characterization

Antibodies

M6792

Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu.

Characterization

Antibodies

M6917

Anti-Macrophage Inflammatory Protein-1α antibody produced in goat, rec.mo.

Characterization

Antibodies

M7273 

Anti-Microtubule-Associated Proteins (MAPs) antibody produced in rabbit

Characterization

Antibodies

M7786

Anti-Smooth muscle myosin, heavy chain, produced in mouse

Characterization

Antibodies

N0649

Anti-Neuronal Specific Enolase antibody produced in rabbit

Characterization

Antibodies

N3038

Monoclonal Anti-Nanog produced in mouse

Characterization

Antibodies

N3279

Monoclonal Anti-Nerve Growth Factor-β antibody produced in mouse

Characterization

Antibodies

N3908

Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in rabbit

Characterization

Antibodies

N4142

Anti-Neurofilament 200 antibody produced in rabbit

Characterization

Antibodies

N6655

Anti-Nerve Growth Factor 2.5S antibody produced in rabbit

Characterization

Antibodies

N6786

Monoclonal Anti-Notch1 antibody produced in mouse

Characterization

Antibodies

N8523

Monoclonal Anti-NF-κB antibody produced in mouse

Characterization

Antibodies

N8773

Anti-Nerve Growth Factor-β antibody produced in goat

Characterization

Antibodies

N9773

Anti-Neurotrophin-3 antibody produced in goat

Characterization

Antibodies

P0873

Anti-Oct4 produced in rabbit

Characterization

Antibodies

P2110

Anti-Platelet-Derived Growth Factor Receptor α antibody produced in goat

Characterization

Antibodies

P2229

Anti-Platelet-Derived Growth Factor Soluble Receptor β antibody produced in goat

Characterization

Antibodies

P5498

Anti-Pro-NGF antibody produced in rabbit

Characterization

Antibodies

P5976

Anti-Platelet-Derived Growth Factor-BB antibody produced in goat

Isolation

Product Number

Characterization

Product Name

General Stem Cell Biology

Workflow

Anti-Smooth Muscle Actin Immunohistology Kit

Expansion

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Functional Profiling

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General Stem Cell Biology

Product List, cont. Workflow

Product Class

Characterization

Antibodies

P6101

Anti-Platelet-Derived Growth Factor antibody produced in goat

Characterization

Antibodies

P6847

Monoclonal Anti-Interleukin-2−R-Phycoerythrin antibody produced in mouse

Characterization

Antibodies

R125

Anti-RANTES antibody produced in goat

Characterization

Antibodies

R2903

Anti-RALT/Mig-6 (SG-16) antibody

Characterization

Antibodies

R3028

Anti-RALT/MIG-6 (PE-16) antibody

Characterization

Antibodies

R5027

Anti-RIP140 antibody produced in rabbit

Characterization

Antibodies

R9528

Anti-RUNX1 antibody

Characterization

Antibodies

S192

Anti-SDF-1/PBSF antibody produced in goat

Characterization

Antibodies

S193

Anti-Synapsin I antibody produced in rabbit

Characterization

Antibodies

S2562

Anti-Stem Cell Factor Receptor (SCF R) antibody

Characterization

Antibodies

S2644

Anti-S-100 antibody produced in rabbit

Characterization

Antibodies

S2687

Anti-SDF-1β/PBSF antibody, recombinant human SDF-1β/PBSF expressed in E. coli, produced in goat, affinity isolated antibody

Characterization

Antibodies

S2822

Anti-Synapsin II antibody produced in rabbit

Characterization

Antibodies

S2947

Anti-Synapsin II (C-terminal) antibody produced in rabbit

Characterization

Antibodies

S4072

Anti-Sirt3 antibody produced in rabbit

Characterization

Antibodies

S4947

Anti-Sirt5 antibody produced in rabbit

Characterization

Antibodies

S5313

Anti-Sir2 (AS-16) antibody produced in rabbit

Characterization

Antibodies

S5947

Anti-Sirt7 antibody produced in rabbit

Characterization

Antibodies

S7693 

Anti-Sox2 antibody produced in rabbit 

Characterization

Antibodies

S7696

Anti-Sec23 antibody produced in rabbit

Characterization

Antibodies

S8316

Monoclonal Anti-SUV39H1 Histone Methyltransferase antibody produced in mouse ~2 mg/mL, clone 44.1, purified immunoglobulin, buffered aqueous solution

Characterization

Antibodies

T0198

Monoclonal Anti-β-Tubulin antibody produced in mouse, ~1 mg/mL, clone D66, purified immunoglobulin, buffered aqueous solution

Characterization

Antibodies

T0563

Anti-Transforming Growth Factor-α antibody, produced in goat

Characterization

Antibodies

T0688

Anti-Transforming Growth Factor-β1 antibody produced in chicken

Characterization

Antibodies

T0951

Anti-Tet Repressor (TETR) antibody produced in rabbit

Characterization

Antibodies

T1940

Anti-Transforming Growth Factor-β Soluble Receptor III antibody produced in goat

Characterization

Antibodies

T1941

Anti-TrkB antibody produced in goat

Characterization

Antibodies

T2200

Anti-β-Tubulin III antibody produced in rabbit

Characterization

Antibodies

T2565

Anti-Transforming Growth Factor-β Soluble Receptor II antibody produced in goat

Characterization

Antibodies

T2817

Anti-Tie 1 antibody produced in goat affinity isolated antibody

Characterization

Antibodies

T3192 

Anti-Tie 2 antibody produced in goat 

Characterization

Antibodies

T4026

Monoclonal Anti-β-Tubulin antibody produced in mouse, clone TUB 2.1, ascites fluid

Characterization

Antibodies

T4442

Anti-Transforming Growth Factor-β2 antibody produced in goat

Characterization

Antibodies

T4692

Anti-Transforming Growth Factor-β3 antibody produced in goat

Characterization

Antibodies

T4817

Anti-Transforming Growth Factor-β5 antibody produced in goat

Characterization

Antibodies

T4930

Anti-Thrombopoietin antibody produced in goat

Characterization

Antibodies

T5076

Monoclonal Anti-β-Tubulin Isotype III antibody produced in mouse, ~2 mg/mL, clone SDL.3D10, purified immunoglobulin, buffered aqueous solution

Characterization

Antibodies

T5201

Monoclonal Anti-β-Tubulin antibody produced in mouse, ~2 mg/mL, clone Tub2.1, purified immunoglobulin, buffered aqueous solution

Characterization

Antibodies

T5293

Monoclonal Anti-β-Tubulin antibody produced in mouse, clone 2-28-33, ascites fluid

Characterization

Antibodies

T5442

Anti-ADAM-17 (TACE), C-Terminal antibody produced in rabbit

Characterization

Antibodies

T7576

Anti-TGN46 antibody produced in rabbit

Characterization

Antibodies

T8660

Monoclonal Anti-β-Tubulin III antibody produced in mouse, clone SDL.3D10, ascites fluid

Characterization

Antibodies

T9429

Anti-Transforming Growth Factor-β, pan antibody produced in rabbit

Isolation

10

sigma-aldrich.com

Characterization

ORDER: 800-325-3010

Product Number

Expansion

Product Name

Differentiation

TECHNICAL SERVICE: 800-325-5832

Functional Profiling

In vivo Tracking

Product Class

Characterization

Antibodies

T9826

Anti-TGN38 antibody produced in rabbit

Characterization

Antibodies

U3254

Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat

Characterization

Antibodies

V1014

anti-Vascular Endothelial Growth Factor Receptor-2 from goat

Characterization

Antibodies

V1014

Anti-Flk1, Vascular Endothelial Growth Factor Receptor 2 antibody

Characterization

Antibodies

V1514

Anti-VE-Cadherin antibody produced in rabbit

Characterization

Antibodies

V2258

Monoclonal Anti-Vimentin antibody produced in mouse, clone LN-6, ascites fluid

Characterization

Antibodies

V4630

Anti-Vimentin antibody produced in goat, whole antiserum

Characterization

Antibodies

V5255

Monoclonal Anti-Vimentin antibody produced in mouse , clone VIM-13.2, ascites fluid

Characterization

Antibodies

V5764

Anti-Vesicular GABA Transporter antibody produced in rabbit

Characterization

Antibodies

V6389

Monoclonal Anti-Vimentin antibody produced in mouse, ~1 mg/mL, clone V9, purified immunoglobulin, buffered aqueous solution

Characterization

Antibodies

V6630

Monoclonal Anti-Vimentin antibody produced in mouse, clone V9, ascites fluid

Characterization

Antibodies

V7881

Monoclonal Anti-Vitronectin antibody produced in mouse

Characterization

Antibodies

W2141

Anti-Wnt-1 antibody produced in goat

Characterization

Antibodies

W2266

Anti-Wnt-4 antibody produced in goat

Characterization

Antibodies

W2516

Monoclonal Anti-Wnt-3a antibody produced in mouse

Characterization/Differentiation

Marker/Receptors

F8674

Fas/Fc Chimera human, recombinant, expressed in mouse NSO cells

Characterization/Differentiation

Marker/Receptors

F8799

Fas/Fc Chimera from mouse, recombinant, expressed in Sf21 cells

Characterization/Differentiation

Proteins/Peptides

A1229

Angiopoietin-3 from mouse recombinant, expressed in mouse NSO cells

Characterization/Differentiation

Proteins/Peptides

A1479

Angiopoietin-4 human recombinant, expressed in mouse NSO cells,

Characterization/Differentiation

Proteins/Peptides

A3156

Bovine Serum Albumin, globulin-free, g-irradiated

Characterization/Differentiation

Proteins/Peptides

A3782

Human Serum Albumin, fatty acid-free, 99%

Characterization/Differentiation

Proteins/Peptides

A4941

Activin A, recombinant, human

Characterization/Differentiation

Proteins/Peptides

A5226

Angiopoietin-1 human, recombinant, expressed in mouse NSO cells

Characterization/Differentiation

Proteins/Peptides

A5843

Human Serum Albumin, low endotoxin

Characterization/Differentiation

Proteins/Peptides

A6955

Angiogenin human, recombinant, expressed in Escherichia coli

Characterization/Differentiation

Proteins/Peptides

A7223

Human Serum Albumin, recombinant, from pichia pastoris

Characterization/Differentiation

Proteins/Peptides

A7979

Bovine Serum Albumin, 35% solution in DPBS

Characterization/Differentiation

Proteins/Peptides

A8806

Bovine Serum Albumin, fatty acid-free

Characterization/Differentiation

Proteins/Peptides

A9847

Angiopoietin-2 human, recombinant, expressed in mouse NSO cells

Characterization/Differentiation

Proteins/Peptides

A9975

ADAM-10 from mouse, recombinant, expressed in Sf21 cells

Characterization/Differentiation

Proteins/Peptides

B1182

Bcl-2 human recombinant, expressed in Escherichia coli

Characterization/Differentiation

Proteins/Peptides

B1436

BAX Inhibiting Peptide V5

Characterization/Differentiation

Proteins/Peptides

E2153

E-Cadherin/Fc Chimera from mouse

Characterization/Differentiation

Proteins/Peptides

H0786

Heregulin-β, EGF Domain human, recombinant, expressed in Escherichia coli

Characterization/Differentiation

Proteins/Peptides

H5529

Heregulin-α, EGF Domain human, recombinant, expressed in Escherichia coli

Characterization/Differentiation

Proteins/Peptides

H7660

Heregulin-β1 (EGF Domain) human, recombinant, expressed in Escherichia coli

Characterization/Differentiation

Proteins/Peptides

I3265

Interferon-γ human

Characterization/Differentiation

Proteins/Peptides

I3275

Interferon-γ from rat

Characterization/Differentiation

Proteins/Peptides

I4151

Interferon β 1a human

Characterization/Differentiation

Proteins/Peptides

I4276

Interferon-αA human

Characterization/Differentiation

Proteins/Peptides

I4401

Interferon-αA/D human

Characterization/Differentiation

Proteins/Peptides

I4777

Interferon-γ from mouse

Characterization/Differentiation

Proteins/Peptides

I4784

Interferon from human leukocytes

Characterization/Differentiation

Proteins/Peptides

I8654

Interferon-γ porcine

Characterization/Differentiation

Proteins/Peptides

I8657

Interferon α from rat

Isolation

Product Number

Characterization

Product Name

Expansion

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Differentiation



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General Stem Cell Biology

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General Stem Cell Biology

Product List, cont. Workflow

Product Class

Product Number

Product Name

Characterization/Differentiation

Proteins/Peptides

I8782

Interferon αA from mouse

Characterization/Differentiation

Proteins/Peptides

I8907

Interferon β from rat

Characterization/Differentiation

Proteins/Peptides

I9032

Interferon β from mouse

Characterization/Differentiation

Proteins/Peptides

n6784

Noggin, mouse recombinant

Characterization/Differentiation

Proteins/Peptides

N8911

Notch-3/Fc Chimera from mouse

Characterization/Differentiation

Proteins/Peptides

N9036

Notch-3/Fc Chimera human

Characterization/Differentiation

Proteins/Peptides

N9161

Notch-2/Fc Chimera from rat

Characterization/Differentiation

Proteins/Peptides

N9268

Notch-1/Fc Chimera from rat

Characterization/Differentiation

Proteins/Peptides

O8137

Osteoprotegerin/Fc Chimera from mouse

Characterization/Differentiation

Proteins/Peptides

O9631

Osteoprotegerin/Fc Chimera human

Expansion/Differentiation

Adhesion/Attachment/Matrices

C0543

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Expansion/Differentiation

Adhesion/Attachment/Matrices

C1809

Collagen from kangaroo tail

Expansion/Differentiation

Adhesion/Attachment/Matrices

C3867

Collagen, Type I solution from rat tail Approx. 100mg/vial

Expansion/Differentiation

Adhesion/Attachment/Matrices

C5533

Collagen from human placenta

Expansion/Differentiation

Adhesion/Attachment/Matrices

C7661

Collagen I from rat tail

Expansion/Differentiation

Adhesion/Attachment/Matrices

C8919

Collagen from calf skin solution, sterile-filtered, 1 mg/mL

Expansion/Differentiation

Adhesion/Attachment/Matrices

C9301

Collagen from chicken sternal cartilage powder

Expansion/Differentiation

Adhesion/Attachment/Matrices

C9791

Collagen from calf skin powder

Expansion/Differentiation

Adhesion/Attachment/Matrices

E1270

ECM gel from Engelbreth-Holm-Swarm murine sarcoma

Expansion/Differentiation

Adhesion/Attachment/Matrices

E2153

E-Cadherin/Fc Chimera from mouse

Expansion/Differentiation

Adhesion/Attachment/Matrices

E2278

E-Cadherin/Fc Chimera human

Expansion/Differentiation

Adhesion/Attachment/Matrices

F0635

Fibronectin from rat plasma powder

Expansion/Differentiation

Adhesion/Attachment/Matrices

F0895

Fibronectin from human plasma 0.1% (Solution),

Expansion/Differentiation

Adhesion/Attachment/Matrices

F1141

Fibronectin from bovine plasma solution, 1 mg/mL

Expansion/Differentiation

Adhesion/Attachment/Matrices

F2006

Fibronectin from human plasma

Expansion/Differentiation

Adhesion/Attachment/Matrices

F2518

Fibronectin from human foreskin fibroblasts Cellular

Expansion/Differentiation

Adhesion/Attachment/Matrices

F4759

Fibronectin from bovine plasma lyophilized powder

Expansion/Differentiation

Adhesion/Attachment/Matrices

H4777

Heparan sulfate proteoglycan

Expansion/Differentiation

Adhesion/Attachment/Matrices

L2020

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Expansion/Differentiation

Adhesion/Attachment/Matrices

L6274

Laminin from human placenta

Expansion/Differentiation

Adhesion/Attachment/Matrices

O2260

Osteopontin from mouse, recombinant, expressed in mouse NSO cells

Expansion/Differentiation

Adhesion/Attachment/Matrices

O3514

Osteopontin from bovine milk

Expansion/Differentiation

Adhesion/Attachment/Matrices

O4264

Osteopontin human recombinant, expressed in mouse NSO cells

Expansion/Differentiation

Adhesion/Attachment/Matrices

P5864

Proteoglycan from bovine nasal septum

Expansion/Differentiation

Adhesion/Attachment/Matrices

P5989

Proteoglycan from chicken sternal cartilage

Expansion/Differentiation

Adhesion/Attachment/Matrices

S7439

L-Selectin/Fc Chimera from mouse

Expansion/Differentiation

Adhesion/Attachment/Matrices

V0132

Vitronectin, from rat plasma

Expansion/Differentiation

Adhesion/Attachment/Matrices

V8379

Vitronectin from human plasma

Expansion/Differentiation

Adhesion/Attachment/Matrices

V9881

Vitronectin from bovine plasma

Expansion/Differentiation

Antibiotics

P4333

streptomycin/penicillin G, 100 ×, stabilized

Expansion/Differentiation

Antibiotics

G1272

Gentamicin solution, 10 mg/mL

Expansion/Differentiation

Antibiotics

P0781

Penicillin-Streptomycin, 100 ×

Expansion/Differentiation

Classical Media

D5796

DMEM

Expansion/Differentiation

Classical Media

D8062

DMEM

Expansion/Differentiation

Classical Media

D8537

DPBS without Ca and Mg

Isolation

12

sigma-aldrich.com

Characterization

ORDER: 800-325-3010

Expansion

Differentiation

TECHNICAL SERVICE: 800-325-5832

Functional Profiling

In vivo Tracking

Product Class

Expansion/Differentiation

Classical Media

I3390

IMDM

Expansion/Differentiation

Classical Media

M0644

MEM

Expansion/Differentiation

Classical Media

M4530

Med 199

Expansion/Differentiation

Classical Media

M4655

MEM

Expansion/Differentiation

Classical Media

M8042

MEM

Expansion/Differentiation

Classical Media

R8758

RPMI

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

B1434

Bone Morphogenetic Protein 7 human, recombinant, expressed in CHO cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

B2680

BMP-4

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

B3555

Bone Morphogenetic Protein 2 (BMP-2) human, recombinant, expressed in Escherichia coli, cell culture tested

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

B3795

Brain-derived neurotrophic factor human recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

C3710

Ciliary Neurotrophic Factor human, ≥97% (SDS-PAGE), recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

C3835

Ciliary Neurotrophic Factor from rat, ≥98% (SDS-PAGE and HPLC), recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

C8615

CXCL16 human recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

E1257

Epidermal Growth Factor from murine submaxillary gland, cell culture tested

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

E1388

β-Endothelial Cell Growth Factor human recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

E4127

Epidermal Growth Factor from murine submaxillary gland

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

E4269

LONG™ EGF human, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

E4643

Heparin-Binding EGF-Like Growth Factor human recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

E8399

Eotaxin-3 human, recombinant, expressed in Escherichia coli,

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

e8403

Eotaxin−Biotin from mouse

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

E9152

Eotaxin-2 from mouse, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

E9644

Epidermal Growth Factor human recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F0291

Fibroblast Growth Factor-Basic human (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F1168

Fibroblast Growth Factor-9 human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F1802

Fibroblast Growth Factor-8c from mouse

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F1927

Fibroblast Growth Factor Receptor 2β (IIIc)/Fc Chimera from mouse

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F2052

Fibroblast Growth Factor Receptor 2β (IIIb)/Fc Chimera from mouse

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

f3133

Fibroblast Growth Factor from bovine pituitary

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F3422

Flt-3/Flk-2 ligand human, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F4537

Fibroblast Growth Factor-5 human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F4662

Fibroblast Growth Factor-6 human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F5392

Fibroblast Growth Factor-Basic from bovine pituitary (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F5542

Fibroblast Growth Factor-Acidic human

Isolation

Product Number

Characterization

Product Name

Expansion

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Differentiation



Functional Profiling

Shaping the Future of Life Science

General Stem Cell Biology

Workflow

In vivo Tracking

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General Stem Cell Biology

Product List, cont. Workflow

Product Class

Product Number

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F5895

Fibroblast Growth Factor Basic Fragment 1-24 bovine (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F6926

Fibroblast Growth Factor-8b from mouse

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F7176

Fibroblast Growth Factor-17 human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F7301

Fibroblast Growth Factor-18 human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F8424

Fibroblast Growth Factor-4 human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F8675

Fibroblast Growth Factor Receptor-4/Fc Chimera human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F8924

Fibroblast Growth Factor 10 human, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F9174

Fibroblast Growth Factor Receptor-1α (IIIc)/Fc Chimera human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F9175

Flt-3/Flk-2 Ligand from mouse (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F9299

Fibroblast Growth Factor Receptor-1β (IIIc)/Fc Chimera human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F9424

Fibroblast Growth Factor Receptor-2α (IIIb)/Fc Chimera human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F9549

Fibroblast Growth Factor Receptor-2α (IIIc)/Fc Chimera human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F9647

Fibroblast Growth Factor Receptor 2β (IIIc)/Fc Chimera from mouse

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F9786

Fibroblast Growth Factor-Basic Heparin Stabilized human (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F9799

Fibroblast Growth Factor Receptor-2β (IIIc)/Fc Chimera human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

F9924

Fibroblast Growth Factor Receptor-3α (IIIc)/Fc Chimera from mouse

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

G0282

Granulocyte-Macrophage Colony-Stimulating Factor from mouse, recombinant, expressed in Escherichia coli, lyophilized powder, ≥97% (SDS-PAGE), cell culture tested (Sigma)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

G0407

Granulocyte Colony-Stimulating Factor

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

G0792

Granulocyte-Macrophage Colony-Stimulating Factor from rat

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

G5035

Granulocyte-Macrophage Colony-Stimulating Factor from human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

G7545

gp130 Soluble Fragment human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

G8160

Granulocyte Colony-Stimulating Factor

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

H1404

HGF, recombinant, human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

H9661

HGF, recombinant, human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I0406

Interleukin-6 from rat recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I0523

Interleukin-2 from mouse, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I0779

Interleukin-2 Soluble Receptor α human, recombinant, expressed in mouse NSO cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I0904

Interleukin-2 Soluble Receptor β human, recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I1029

Interleukin-2 Soluble Receptor γ human, recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I1145

Interleukin-5 from mouse, recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I1271

LONG™ R3 IGF-1, Recombinant Analog human (1)

Isolation

14

sigma-aldrich.com

Characterization

ORDER: 800-325-3010

Expansion

Product Name

Differentiation

TECHNICAL SERVICE: 800-325-5832

Functional Profiling

In vivo Tracking

Product Class

Product Number

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I1395

Interleukin-6 human recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I1645

Interleukin-8 human, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I1646

Interleukin-3 human recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I2276

Interleukin-12 human, recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I2393

Interleukin-1β from rat, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I2406

Interleukin-11 human, recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I2526

Insulin-like Growth Factor-II human (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I2644

Interleukin-2 human, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

i2656

Insulin-like Growth Factor-I (E3R) human (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I2778

Interleukin-1α human, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I3263

Insulin-like Growth Factor-II fragment 33-40 (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I3268

Interleukin-6 human from human (osteosarcoma cells),

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I3657

Insulin-Like Growth Factor Binding Protein-2 from mouse (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I3769

IGF-1, recombinant, human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I3901

Interleukin-1α from rat, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I4144

Interleukin-3 from mouse recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I4638

Interleukin-1β Fragment 163-171 human, synthetic

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I4892

Interleukin-7 from mouse, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I5271

Interleukin-1β from mouse, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I5273

Interleukin-5 human, recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I5277

Interferon-γ Receptor 1 human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I5396

Interleukin-1α from mouse, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I5403

Insulin-Like Growth Factor Binding Protein-2 human (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I5771

Interleukin-6 Receptor Soluble Fragment human recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I5896

Interleukin-7 human, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I7389

Interleukin-3 human recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I7908

Interleukin-2 human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I8148

Interleukin-1 Soluble Receptor, Type II human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I8523

Interleukin-12 from mouse, recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I8779

Insulin-like Growth Factor-I from mouse (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I8904

Insulin-like Growth Factor-II from mouse (1)

Isolation

Characterization

Product Name

Expansion

Our Innovation, Your Research

Differentiation



Functional Profiling

Shaping the Future of Life Science

General Stem Cell Biology

Workflow

In vivo Tracking

15

General Stem Cell Biology

Product List, cont. Workflow

Product Class

Product Number

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I9279

Interleukin-11 from mouse, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I9401

Interleukin-1β human, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I9646

Interleukin-6 from mouse recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I9780

Interleukin-2/Fc Chimera (non-lytic) human, recombinant, expressed in NS.1 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

I9905

Interleukin-2/Fc Chimera (non-lytic) from mouse, recombinant, expressed in CHO cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

L3408

Transforming Growth Factor-β1 Latency Associated Peptide human, recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

L5158

LIF (Leukemia Inhibitory Factor), recombinant, mouse

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

L5283

LIF (Leukemia Inhibitory Factor), recombinant, human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

M1439

MIG from mouse

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

M252

MIG human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

M261

Platelet-Derived Growth Factor-BB from rat

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

M6167

Macrophage Inflammatory Protein-1α from mouse, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

M6292

Macrophage Inflammatory Protein-1α human, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

M6417

Macrophage Inflammatory Protein-1β human, recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

M6542

Macrophage Inflammatory Protein-1β from mouse, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

N0513

Nerve Growth Factor-7S from murine submaxillary gland

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

N1408

Nerve Growth Factor-β human recombinant

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

N1780

Neurotrophin-4 human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

N1905

Neurotrophin-3 human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

N2513

Nerve Growth Factor-β from rat recombinant

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

N6009

Nerve Growth Factor-2.5S from murine submaxillary gland

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

N8133

Nerve Growth Factor from Vipera lebetina venom

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

N8898

Nerve Growth Factor Receptor/Fc Chimera human recombinant

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

N9411

Nerve growth factor receptor/Fc chimera from mouse recombinant

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

P1985

Platelet-Derived Growth Factor Soluble Receptor α human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

P3076

Platelet-Derived Growth Factor-AA human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

P3201

Platelet-Derived Growth Factor-BB human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

P3326

Platelet-Derived Growth Factor-AB human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

P3618

Platelet-Derived Growth Factor Receptor β/Fc Chimera human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

P4056

Platelet-Derived Growth Factor-BB from rat

Isolation

16

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Characterization

ORDER: 800-325-3010

Expansion

Product Name

Differentiation

TECHNICAL SERVICE: 800-325-5832

Functional Profiling

In vivo Tracking

Product Class

Product Number

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

P4306

Platelet-Derived Growth Factor-BB human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

P8147

Platelet-Derived Growth Factor from human platelets

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

P8953

Platelet-Derived Growth Factor from porcine

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

R2274

RANTES from mouse

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

R6267

RANTES human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

S190

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor human, recombinant, expressed in Escherichia coli,

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

S2437

SCF Soluble Receptor human

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

S5816

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

S7901

Stem Cell Factor human recombinant

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

S8406

Stromal Cell-Derived Factor 1β/pre-B Cell Growth Stimulating Factor human, recombinant, expressed in Escherichia coli,

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

S9915

Stem Cell Factor from mouse recombinant

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T0892

Interleukin-2 from rat spleenocytes

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T1566

Tie-1/Fc Chimera human, recombinant, expressed in mouse NSO cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T1654

Transforming Growth Factor-β1 from human platelets

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T2815

Transforming Growth Factor-β2 human, recombinant, expressed in NSO cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T2926

Transforming Growth Factor-β5 amphibian, recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T3448

Tie-2/Fc Chimera from mouse, recombinant, expressed in mouse NSO cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T3698

Transforming Growth Factor-β Receptor II/Fc Chimera from mouse, recombinant, expressed in mouse NSO cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T4567

Transforming Growth Factor-β Soluble Receptor III human, recombinant, expressed in mouse NSO cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T5050

Transforming Growth Factor-β1 from porcine platelets

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T5278

Transforming Growth Factor-α from rat, g-irradiated

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T5300

Transforming Growth Factor-β2 from porcine platelets

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T5425

Transforming Growth Factor-β3 human, recombinant, expressed in Sf21 cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T7039

Transforming Growth Factor-β1 human, recombinant, expressed in insect cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T7039

Transforming Growth Factor-β1 human, recombinant, expressed in insect cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T7924

Transforming Growth Factor-α human, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T8569

TrkC/Fc Chimera human recombinant, expressed in mouse NSO cells

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T8694

TrkB/Fc Chimera human recombinant, expressed in mouse NSO cells,

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

T9533

Transforming Growth Factor-α from rat

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

V1260

VEGF Receptor-3 (Flt-4)/Fc Chimera human

Isolation

Characterization

Product Name

Expansion

Our Innovation, Your Research

Differentiation



Functional Profiling

Shaping the Future of Life Science

General Stem Cell Biology

Workflow

In vivo Tracking

17

General Stem Cell Biology

Product List, cont. Workflow

Product Class

Product Number

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

V3388

Vascular Endothelial Growth Factor 121 human (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

V3513

Vascular Endothelial Growth Factor 120 from mouse (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

V3638

Vascular Endothelial Growth Factor 164 from rat (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

V4512

Vascular Endothelial Growth Factor from mouse (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

V6012

Vascular Endothelial Growth Factor D human (1)

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

V6633

VEGF Receptor-3 (Flt-4)/Fc Chimera from mouse

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

V6758

VEGF Receptor-2(Flk-1, KDR)/Fc ChimeraVEGF Receptor-2

Expansion/Differentiation

Cyokines/Growth Factors/ Chemokines

V7259

Vascular Endothelial Growth Factor human (1)

Expansion/Differentiation

Enzymes

F2803

Flt-3/Fc Chimera, rec mo

Expansion/Differentiation

Enzymes

F7426

Flt-3/Fc Chimera human, recombinant, expressed in mouse NSO cells,

Expansion/Differentiation

Enzymes

L0164

LYN B human, recombinant, expressed in insect cells

Expansion/Differentiation

Enzymes

L9913

LYN A human, recombinant, expressed in insect cells

Expansion/Differentiation

Specialty Media

51800C

Expansion/Differentiation

Stemline Media

S0189

Stemline HSC

Expansion/Differentiation

Stemline Media

S0192

Stemline HSC

Expansion/Differentiation

Stemline Media

S0196

Stemline Keratinocyte

Expansion/Differentiation

Stemline Media

S1569

Stemline MSC

Expansion/Differentiation

Stemline Media

S1694

Stemline T cell

Expansion/Differentiation

Stemline Media

S3194

Stemline Neural Stem Cell Expansion medium

Expansion/Differentiation

Stemline Media

S3444

Stemline DC

Expansion/Differentiation

Stemline Media

S9945

Stemline Keratinocyte Medium Supplement with BPE

Expansion/Differentiation

Vitamins, Hormones, Misc.

A4034

sodium ascorbate (Takahashi, 2003)

Expansion/Differentiation

Vitamins, Hormones, Misc.

A6791

Atrial Natriuretic Peptide fragment 3-28 rat

Expansion/Differentiation

Vitamins, Hormones, Misc.

D4902

Dexamethasone, cell culture tested

Expansion/Differentiation

Vitamins, Hormones, Misc.

D8893

Dexamethasone, γ-irradiated, cell culture tested

Expansion/Differentiation

Vitamins, Hormones, Misc.

R0635

Retinol Acetate - soluble

Expansion/Differentiation

Vitamins, Hormones, Misc.

R2625

retinoic acid

Expansion/Differentiation

Vitamins, Hormones, Misc.

R7882

Retinol Acetate

Expansion/Differentiation

Vitamins, Hormones, Misc.

T1568

Thrombopoietin human, recombinant, expressed in Escherichia coli

Expansion/Differentiation

Vitamins, Hormones, Misc.

T4184

Thrombopoietin from mouse, recombinant, expressed in NSO cells

Functional Profiling

Inhibitors

P4484

Purvalanol A

Functional Profiling

Ion Channels

17783

Calcein-AM

Functional Profiling

Kit

BLOT2

Northern Blots, poly(A)+ RNA from BALB/c mouse, multiple tissue

Functional Profiling

Kit

BLOT3

Northern Blots, poly(A)+ RNA from BALB/c murine embryo

Functional Profiling

Kit

BPQ24

BioPORTER® QuikEase™ Protein Delivery Kit

Functional Profiling

Kit

BPQ96

BioPORTER QuikEase Protein Delivery Kit

Functional Profiling

Kit

CSAA1

Panorama® Antibody Microarray - Cell Signaling Kit

Functional Profiling

Kit

D7440

JumpStart™ Taq ReadyMix™ for Quantitative PCR

Functional Profiling

Kit

DMN10

GenElute™ Direct mRNA Miniprep Kit sufficient for 10 purifications

Functional Profiling

Kit

DNAQF

DNA Quantitation Kit, Fluorescence Assay

Functional Profiling

Kit

G1N10

GenElute Mammalian Genomic DNA Miniprep Kit sufficient for 10 purifications

Functional Profiling

Kit

GRAA2

Panorama Antibody Microarray - Gene Regulation I Kit

Isolation

18

sigma-aldrich.com

Characterization

ORDER: 800-325-3010

Expansion

Product Name

Claycomb medium

Differentiation

TECHNICAL SERVICE: 800-325-5832

Functional Profiling

In vivo Tracking

Workflow

Product Class

Functional Profiling

Kit

HPFM4

Functional Profiling

Kit

ID0010

In vitro Director™ PCR System 3′ FLAG®

Functional Profiling

Kit

ID0020

In vitro Director PCR System 5′ c-Myc

Functional Profiling

Kit

ID0030

In vitro Director PCR System 5′ FLAG

Functional Profiling

Kit

ID0040

In vitro Director PCR System 5′ Anchor™

Functional Profiling

Kit

MRN10

GenElute mRNA Miniprep Kit sufficient for 10 purifications

Functional Profiling

Kit

N5659

Northern Bound™ Human Alzheimer’s Total RNA Blot II from human

Functional Profiling

Kit

N5784

Northern Bound Human mRNA Blot

Functional Profiling

Kit

N5909

Northern Bound Human mRNA Blot II

Functional Profiling

Kit

N7409

Northern Bound Human Normal Brain Total RNA Blot

Functional Profiling

Kit

N7534

Northern Bound Human Normal Brain Total RNA Blot II

Functional Profiling

Kit

NA2000

Functional Profiling

Kit

PPAA4

Panorama Antibody Array -p53 pathways

Functional Profiling

Kit

R2653

RapidTransit™ Transformation Kit

Functional Profiling

Kit

RTN10

GenElute Mammalian Total RNA Miniprep Kit sufficient for 10 purifications

Functional Profiling

Kit

SH0211

MISSION® shRNA Human G-Protein Coupled Receptors, Glycerol Set-1

Functional Profiling

Kit

SH0231

MISSION shRNA Human G-Protein Coupled Receptors, Lentiviral Transduction Particle Set 1

Functional Profiling

Kit

SH0511

MISSION shRNA Human Tumor Suppressors: Glycerol Set-1

Functional Profiling

Kit

SH0531

MISSION shRNA Human Tumor Suppressors: Lentiviral Transduction Particle Set

Functional Profiling

Kit

SH0831

MISSION shRNA Human Cytokines and Chemokines, Lentiviral Transduction Particle Set 1

Functional Profiling

Kit

SH1011

MISSION shRNA Human Ion Channels: Glycerol Set-1

Functional Profiling

Kit

SH1031

MISSION shRNA Human Ion Channels, Lentiviral Transduction Particle Set 1

Functional Profiling

Kit

SH1311

MISSION shRNA Human Cytokine and Chemokine Receptors, Glycerol Set-1

Functional Profiling

Kit

WGA1

GenomePlex® Whole Genome Amplification (WGA) Kit

Functional Profiling

Kit

WGA4

GenomePlex Single Cell Whole Genome Amplification Kit

Functional Profiling

Kit

WTA1

TransPlex® Whole Transcriptome Amplification Kit

Functional Profiling

Kit

XNAB2

Extract-N-Amp™ Blood PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications

Functional Profiling

Kit

XNABRE

REDExtract-N-Amp™ Blood PCR Kit sufficient for 10 extractions, sufficient for 10 amplifications

Functional Profiling

Reagent

D5758

Diethyl pyrocarbonate ≥97% (NMR)

Functional Profiling

Transfection/Transduction

N2913

N-TER Nanoparticle siRNA Transfection System

Functional Profiling

Transfection/Transduction

NPT01

NeuroPorter™ Transfection Kit

Functional Profiling

Transfection/Transduction

SHC002

Functional Profiling

Transfection/Transduction

SHC002V

Functional Profiling

Transfection/Transduction

SHC003

Functional Profiling

Transfection/Transduction

SHC003V

In vitro & In vivo Tracking

Stains/Syes

32857

Wright Stain solution

In vitro & In vivo Tracking

Stains/Syes

D8417

DAPI

In vitro & In vivo Tracking

Stains/Syes

G9641

Giemsa stain

In vitro & In vivo Tracking

Stains/Syes

HT90T

Gram stain for tissue

In vitro & In vivo Tracking

Stains/Syes

M5655

MTT

In vitro & In vivo Tracking

Stains/Syes

P1951

Phalloidin–Tetramethylrhodamine B isothiocyanate Phalloidin from Amanita phalloides

In vitro & In vivo Tracking

Stains/Syes

P5282

Phalloidin, Fluorescein Isothiocyanate Labeled Phalloidin from Amanita phalloides

In vitro & In vivo Tracking

Stains/Syes

T8154

Trypan Blue solution 0.4%, liquid, sterile-filtered, cell culture tested

In vitro & In vivo Tracking

Stains/Syes

X4626

XTT

Characterization

Product Name Panorama® Human Protein Function Array -Signal Transduction

General Stem Cell Biology

Isolation

Product Number

GenElute Blood Genomic DNA Kit sufficient for 10 purifications

MISSION Non-Target shRNA Control Transduction Particles MISSION Non-Target shRNA Control Vector MISSION TurboGFP™ Control Vector MISSION TurboGFP Control Transduction Particles

Expansion

Our Innovation, Your Research

Differentiation



Functional Profiling

Shaping the Future of Life Science

In vivo Tracking

19

General Stem Cell Biology

Stem Cell Marker Table

20

Category

Marker

ASC

Adipose-derived mesenchymal stem cell

ASC, PSC

CXCR4

ASC, PSC

ATP Binding Cassette G2

ASC, PSC

Gene

PN

chemokine cell surface receptor

CXCR4

C8227

Anti-CXCR4 (Fusin), N-Terminal

transporter protein

ABCG2

B7165

Anti-Breast Cancer Resistance Protein

ATP Binding Cassette G2

transporter protein

ABCG2

B7059

Monoclonal Anti-Breast Cancer Resistance Protein

ASC, PSC

ATP Binding Cassette G2

transporter protein

ABCG2

B7684

Monoclonal Anti-Breast Cancer Resistance Protein

AGPC

Glial Fibrillary Acidic Protein

Intermediate filament protein

GFAP

G9269

Anti-Glial Fibrillary Acidic Protein

AGPC

Glial Fibrillary Acidic Protein

Intermediate filament protein

GFAP

G3893

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP)

BSC

Osteoblast mesenchymal stem cell, periosteum progenitor

CM

GATA-4

GATA4

G8044

Anti-phospho-GATA4 (pSer105) antibody produced in rabbit

CM

Cardiac troponin I

EB

Embryoid body

EB, HSC, CM

Flk1

Cell surface receptor

KDR

V1014

Anti-Vascular Endothelial Growth Factor Receptor 2

EB, HSC, CM

Flk1

Cell surface receptor

KDR

V9134

Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2

EB, HSC, CM

Flk1

Cell surface receptor

KDR

V3003

Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)

EGC

Embryonic germ cell

Epithelial SC

Cytokeratin 19

KRT19

C6930

Monoclonal Anti-Cytokeratin Peptide 19

Epithelial SC

p63

Trp63

P3362

Monoclonal Anti-p63

Epithelial SC

p63

Trp63

P3737

Monoclonal Anti-p63

ERC

Endometrial Regenerative Cell

ERC, BSC, MSC, ASC, SMPC

Endoglin (CD105)

TGFbeta coreceptor

ESC

SSEA-3 (stage-specific embryonic antigen)

glycolipid antigen

ESC

SSEA-4 (state-specific embryonic antigen)

glycolipid antigen

ESC

GCT343

karatan sulfate antigen

ESC

GCTM2

karatan sulfate antigen

ESC

Embryonic stem cell

ESC, ASC, ERC, BSC, GSC

CD9

Cell adhesion, motility

CD9

ESC, HSC, MSC, ERC, GSC

Thy-1 (CD90)

GPI-anchored cell surface protein, adhesion

THY1

M7898

Monoclonal Anti-Thy 1.1

ESC, MSC, BSC

tissue nonspecific alkaline phosphatase

ESC, PSC

Rex-1

zinc finger protein

Zfp42

GSC

Integrin alpha 6

Cell adhesion

ITGA6

GSC

Stra8

GSC

Germline stem cell

HSC

CD38

Cell surface receptor, enzyme

C1586

Monoclonal Anti-CD38

HSC

CD45

Cell surface receptor, protein phosphatase

PTPRC

C7556

Monoclonal Anti-CD45

HSC

CD34

Cell surface/cell adhesion

CD34

HSC

c-Kit (CD117 antigen)

Stem Cell Factor receptor

KIT

K0131

Monoclonal Anti-c-kit/SCF Receptor antibody

HSC

c-Kit (CD117 antigen)

Stem Cell Factor receptor

KIT

C6740

Anti-phospho-c-Kit (SCFR) (pTyr703) antibody produced in rabbit

HSC

c-Kit (CD117 antigen)

Stem Cell Factor receptor

KIT

C6615

Anti-phospho-c-Kit (SCFR) (pTyr730) antibody produced in rabbit (1)

HSC

c-Kit (CD117 antigen)

Stem Cell Factor receptor

KIT

C6490

Anti-phospho-c-Kit (SCFR) (pTyr823) antibody produced in rabbit (1)

HSC

c-Kit (CD117 antigen)

Stem Cell Factor receptor

KIT

C6365

Anti-phospho-c-Kit (SCFR) (pTyr936) antibody

HSC

Sca-1

HSC

Hematopoietic stem cell

LSC

CD96

sigma-aldrich.com

ORDER: 800-325-3010

Function/Pathway

Cardiomyocyte differentiation through BMP

SA Antibody

TNNI3

Epithelial intermediate filament protein

ENG

alpl

STRA8

CD96

TECHNICAL SERVICE: 800-325-5832

Marker

Function/Pathway

Gene

PN

LSC

Cytokeratin 18

Epithelial intermediate filament protein

Krt18

C1399

Monoclonal Anti-Cytokeratin Peptide 18

LSC

Cytokeratin 18

Epithelial intermediate filament protein

Krt18

C8541

Monoclonal Anti-Cytokeratin Peptide 18

LSC

Hepatic/biliary stem cells

MPC

Desmin

cytoskeletal protein

SA Antibody

DES

D8281

Anti-Desmin

H1650

Monoclonal Anti-HLA Class I Antigen

C0551

Monoclonal Anti-CD11b

MSC

HLA class I

Antigen processing/presentation

HLA-A, HLA-B, HLA-C

MSC

CD11b (Integrin alpha M)

Cell adhesion

ITGAM

MSC

CD59

Cell adhesion

CD59

MSC

Mesenchymal (stromal) stem cell

MSC, ERC

CD44

Cell adhesion (hyaluronic acid receptor)

CD44

C7923

Monoclonal Anti-CD44

NPC

beta-III Tubulin

microtubule protein

TUBB3

T2200

Anti-β-Tubulin III

NPC

beta-III Tubulin

microtubule protein

TUBB3

T8660

Monoclonal Anti-β-Tubulin III

NPC

beta-III Tubulin

microtubule protein

TUBB3

T5076

Monoclonal Anti-β-Tubulin Isotype III

NPC

Microtubule Associated Protein 2

microtubule protein

MAP2

M2320

Monoclonal Anti-MAP2 (2a+2b)

NPC

Microtubule Associated Protein 2

microtubule protein

MAP2

M9942

Monoclonal Anti-MAP2

NPC

Musashi 1

RNA binding

MSU1

NSC

vimentin

Intermediate filament protein

VIM

V4630

Anti-Vimentin

NSC

vimentin

Intermediate filament protein

VIM

V6389

Monoclonal Anti-Vimentin

NSC

vimentin

Intermediate filament protein

VIM

V5255

Monoclonal Anti-Vimentin

NSC

Nestin

NSC

Neural stem cell

NSC

A2B5

Cell surface ganglioside marker

A8229

Monoclonal Anti-A2B5

NSC, GSC, MSC

Integrin beta1 (CD29)

Cell surface adhesion factor

I7533

Anti-phospho-Integrin β1 (pSer785)

NSC, ESC, PSC, HSC, CSC

CD133/Prominin 1

OLPC

Oligodendrocyte Marker O4

cell surface protein

O7139

Monoclonal Anti-Oligodendrocyte Marker O4

OLPC

Galactosylceramide

mylelin membrane lipid

GALC

G9152

Anti-Galactocerebroside

PESC

Prostate endothelial stem cell

PESC, ECS, EGC, GC

Oct4

Maintains pluripotency

POU5F1

P0873

Anti-POU5F1 (Oct4)

PSC

Nanog

Maintains pluripotency

NANOG

N3038

Monoclonal Anti-Nanog

PSC

SOX2

PSC, EGC, EC

Tra-1-60

karatan sulfate antigen

PSC, EGC, EC

Tra-1-81

karatan sulfate antigen

RPEC

Retinal pigment epithelium

RPC, OPC

MITF

SC

Stem cell

SMPC SMPC SMPC

General Stem Cell Biology

Category

NES

ITGB1 PROM1

SOX2

Transcription factor

MITF

M6065

Monoclonal Anti-Microphthalmia antibody

Smooth muscle myosin

contractile protein

MYH11

M7786

Monoclonal Anti-Myosin (Smooth)

Calponin, basic

cytoskeletal protein

CNN1

C6047

Monoclonal Anti-Calponin

Actin, smooth muscle alpha

cytoskeletal/contractile protein

ACTA2

A5228

Monoclonal Anti-Actin, α-Smooth Muscle

Key:

Astroglia Progenitor Cell

ESC

Embryonic Stem Cells

OPC

Osteoclast Progenitor Cell

ASC

Adipose Stromal Cell

GC

Germ Cells

PESC

Pluripotent Embryonic Stem Cell

BDPC

Bile Duct Progenitor Cell

HSC

Hematopoietic Stem Cell

PESC

Prostate Endothelial Stem Cells

BSC

Blood Stem Cell

LSC

Leukemic Stem Cell

PSC

Pluripotent Stem Cell

CM

Cardiomyocyte

MPC

Mesoderm Progenitor Cell

RPC

Retinal Progenitor Cell

EB

Embryoid Body

MSC

Mesenchymal Stem Cell

RPEC

Retinal pigment Epithelilial cell

EC

Tetracarcinoma Stem Cells

NPC

Neural Progenitor Cell

SMPC

Smooth Muscle Progenitor Cell

EGC

Embryonic Germ Cells

NSC

Neural Stem Cell

ERC

Endometrial Regenerative Cell

OLPC

Oligodendrocyte Progenitor Cells

AGPC

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Antigen

Oligosaccharide structure

Globoseries glycolipids SSEA3

Galβ1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer

SSEA3, SSEA4

NeuNAcα

Forssman

3Galβ1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer GalNAcα1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer

Latoctoseries glycolipids SSEA1

Galβ1 → 4GlcNAcβ1 → 4Galβ1 → 4Glcβ1 → Cer 3 →

General Stem Cell Biology

Glycolipid Antigens of Mouse and Human EC and ES Cells

Fucαl Ganglioseries glycolipids 9-O-acetyl GD3 (ME 311)

(9-O-acetyl) NeuNAcα2 → 8NeuNAcα2 → 3Galβ1 → 4Glcβ1 → Cer

GT3 (A2B5)

NeuNAcα2 → 8NeuNAcα2 → 8NeuNAcα2 → 3Galβ1 → 4Glcβ1 → Cer (adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)

All of these glycosphingolipid antigens are synthesized by extension from a common precursor, lactosylceramide. The likely parts of these structures comprising the epitopes recognized by the different antibodies are highlighted. The enzymes responsible for the addition of the third sugar residues (galactosyl-, glucosaminyl-, and sialyltransferase) appear to regulate the rate-limiting step that controls which synthetic pathway predominates. Human EC and ES cells are characterized by expression of the glycolipid antigens SSEA3 and SSEA4, and not the lactoseries antigen SSEAl (Lex). By contrast, mouse EC and ES cells are typically characterized by their expression of SSEAl. Mouse cells also express the globoseries Forssman antigen, which is not synthesized by human cells. Thus, despite the apparent distinction between mouse and human EC and ES cells in terms of their terminal sugars that comprise the different epitopes, the undifferentiated cells of both species do seem to share in common the expression of globoseries core structures.

Differential Expression of Characteristic Surface Marker Antigens of Mouse and Human EC and ES Cells Antigen

Mouse

Human

Forssman

Yes

No

Globoseries glycolipid

SSEA 1 (Le )

Yes

No

Lactoseries glycolipid

SSEA3

No

Yes

Globoseries glycolipid

SSEA4

No

Yes

Globoseries glycolipid

TRA-1-60

No

Yes

Keratan Sulfate

TRA-1-81

No

Yes

Keratan Sulfate

GCTM2

No

Yes

Keratan Sulfate

Liver alkaline phosphatase (L-ALP)

Yes

Yes

No antibody is available for murine ALP, but human L-ALP is recognized by antibodies TRA-2-54 and TRA-2-49.

MRC Class 1

No

Yes

H-2 in the mouse; HLA in humans

x

Comments

(adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)

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Common Antibodies Used to Detect Antigens Expressed by Human EC and ES Cellsiso SSEA3

Rat IgM

Undifferentiated hES-EC

MC480

SSEA1 (Le )

Mouse IgM

Differentiated hES-EC; including trophoblast

A2B5

GT3

Mouse IgM

Neural

ME311

9-O-acetylGD3

Mouse IgG

Neural

VINI556

GD3

Mouse IgM

Neural

VIN2PB22

GD2

Mouse IgM

Neural

MC8 1 3-70

SSEA4

Mouse IgG

Undifferentiated hES-EC

TRA-1-60

TRA-1-60

Mouse IgM

Undifferentiated hES-EC

TRA-1-81

TRA-1-81

Mouse IgM

Undifferentiated hES-EC

TRA-2-54

L-ALP

Mouse IgG

Undifferentiated hES-EC

TRA-2-49

L-ALP

Mouse IgG

Undifferentiated hES-EC

TRA-1-85

Ok[a]

Mouse IgG

Panhuman

BBM1

β2-microglobulin

Mouse IgG

HLA

x

W6/32 HIA-A, -B, -C Mouse IgG

General Stem Cell Biology

MC631

HLA

(adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)

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NIH Code

Karyotype

AC133

Alk Phos

CD90

Cripto

Cryopreservation

Feeder-free

H1

WA01

XY

+

+

+

+

+

+

H7

WA07

XX

+

+

+

+

+

+

H9

WA09

XX

+

+

+

+

+

HES-1

ES01

XX

+

+

+

+

HES-2

ES02

XX

+

+

+

+

General Stem Cell Biology

ES Line

GCTM-2

HES-3

ES03

XX

+

+

+

+

HES-4

ES04

XY

+

+

+

+

+

+

+

+

HES-6

ES06

XY

HSF-6

UC06

XX

hESBGN.01

BG01

XY

hESBGN.02

BG02

XY

+

Miz-hES-1

MI01

XY

+

ES Marker

Mouse

Human

Alk Phos

POS

POS

BCRP-1

POS

POS

CX43

POS

POS

CX45

POS

POS

FGF-4

POS

POS

FOXD3

POS

NEG

gp130

POS

POS

LIFR

POS

NEG

Nanog

POS

POS

OCT 3/4

POS

POS

REX-1

POS

POS

SOX 2

POS

POS

SSEA-1

POS

NEG

SSEA-3

NEG

POS

SSEA-4

NEG

POS

STAT3

POS

POS

TERT

POS

POS

TRA-1-60

NEG

POS

TRA-1-81

NEG

POS

UTF-1

POS

POS

+

1981, ES Cell First isolated in mice

1995, First non-human p

200

2

2000, NIH

1998, First hum

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GDF3

Genesis

hTERT

Nanog

OCT4

REX-1

SOX 2

SSEA-3

SSEA-4

+

+

+

+

+

+

+

+

+

+

+

+

TG343

TRA-1-60

TRA-1-81

UTF-1

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+/-

+

+

+

+

+

+

+

+

+

+

+

+

+

General Stem Cell Biology

+

+

primate ES line

00, NIH issue guidelines for Federal funding of ESC research

2001, Federal Government announces restrictions on funding for Stem Cell Research 2003, Ohio establishes the Center for Stem Cell and Regenerative Medicine 2003 - 2007, IL, MD, CT, IN, and other states pass legislation for state funding

2007, ES-like cells derived from skin fibroblasts 2004, CA and NJ appropriate funding at the state level to support SC Research 2003, Ohio establishes the Center for Stem Cell and Regenerative Medicine

H issue guidelines for Federal funding of ESC research

man ES line

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Stem Cell Biology General Stem Cell Biology

Where we’ve been and where we’re heading! By: Dr. Greg Wall, PhD, BioChemsultants, LLC. Unraveling and exploring the mysteries of life have been driving forces behind scientific studies since Aristotle first attempted to understand the origins of life. Through the eyes of the microscope, cellular processes have been observed, investigated, understood, and manipulated. This quest has intensified with the discovery of embryonic and adult stem cells. In recent years the genetic modification of stems cells, and the latest discovery, the transformation of somatic cells into stem cells have emerged as primary areas of therapeutic biomedical research. Potentially, stem cells can be transformed into any of the over 200 specialized cells in the body. Their use in cell-based therapies, drug development, and new disease models is still in its infancy. The future potential in basic and applied clinical research is fueled by over 2,000 articles published yearly. Since the 1950s, adult stem cells have been used in bone marrow transplants and to develop a variety of treatments for diseases. Bone marrow transplants are used to treat malignant diseases such as acute and chronic leukemias, Hodgkin's disease and non-Hodgkin's lymphoma, myelodysplasia, multiple myeloma, myelofibrosis, and renal cell carcinoma. Increasingly, success has been seen in the treatment other diseases including aplastic anemia, sickle cell anemia, immune deficiency disorders, and autoimmune diseases such as scleroderma and multiple sclerosis. It was not until 1963 that the first qualitative description of bone marrow cell’s self-renewing activities by Ernest McCulloch and James Till, Ontario Cancer Institute, established the concept of stem cells.1 The first embryonic stem cells derived directly from the blastocyst in culture was reported by Martin Evans and Matthew Kaufman, University of Cambridge, in 1981.2 During the 1980s and 1990s, techniques for targeting and altering genetic material and in growing human cells in the laboratory flourished and laid the groundwork for human stem cell research. It was observed that telomerase activity is expressed in unspecialized stem cells and progenitor cells, but suppressed in somatic cells, and specific cell surface antigens SSEA-3, SSEA-4, Tra-1-60 and Tra-1-81 can be used to identify stem cells. The first cloning of a mammal by somatic cell nuclear transfer (SCNT) in 1997 by Ian Wilmut, Roslin Institute, demonstrated mammalian differentiated cells can be transformed to an undifferentiated state by trans-acting factors present in the oocyte. This was followed by the search for factors that could mediate similar reprogramming of somatic cells.3 The following year, James Thomson, University of Wisconsin – Madison, used trans-acting factors to reprogram isolated cells from the inner cell mass of early embryos, leading to the development of the first human stem cell lines and John Gearhart, Johns Hopkins University, derived human embryonic germ cells from primordial germ cells.4,5 Pluripotent stem cell lines were then prepared from both. Subsequent research has yielded a better understanding of the characteristics, growth, and differentiating properties of adult and embryonic stem cells. Researchers are now beginning to identify the transcription factors necessary to reprogram human somatic cells to induced pluripotent stem cells.

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The reprogramming and induction of human somatic cells to stem cells that exhibit the characteristics of embryonic stem cells have been pursued by many laboratories and was reported in separate papers by Shinya Yamanaka, Kyoto University, and James Thomson, University of Wisconsin – Madison, in 2007. Their findings were based on the similarity to human embryonic stem cells in morphology, proliferation mechanisms, surface antigens, pluripotent cell-specific gene epigenetic status, and telomerase activity. Yamanaka utilized Oct3/4, Sox2, Kif4, and c-Myc transcription factors to reprogram human dermal fibroblast cells. In this method, c-Myc and Klf4 may be involved in modifying chromatin structures so that Oct3/4 and Sox2 bind to their targets. Thomson transduced IMR90 fibroblast and postnatal fibroblast cells with a combination of Oct4, Sox2, Lin28, and NANOG. Lin28 and NANOG have been shown to improve cloning efficiency and recovery. The induced pluripotent human stem cells by both methods maintained their ability to differentiate into all three germ layers in vitro. One potential limitation of the Yamanaka method using the c-Myc factor would be mutation through viral integration. The altered or forced expression of c-Myc may result in unwanted differentiation of the induced pluripotent stem cells. By using different transcription factors to reprogram and generate cells having the same characteristics as stem cells, the viability of this technology has been validated as a method to induce somatic cells. The induction process and how to harness it is now under further investigation. Paralleling this achievement, research groups have continued to make progress in developing methods to improve the efficiency of human therapeutic cloning via SCNT. Advances have been made in the preparation of recipient cytoplasts, preparation and transfer of donor DNA, and parthenogenic activation of embryonic development. Recently, Andrew French, Stemagen Corporation, has been able to clone blastocysts from somatic cells.8 The ability to prepare nuclear transfer stem cells by this method is promising to regenerative medicine and cell-based drug discovery. The preparation of human induced pluripontent stem cells is a milestone in stem cell biotechnology as is its impact on medical applications. This opens up the possibility of using them as well as embryonic stems cells in treating medical conditions such as cancer and birth defects, along with generating tissue for arthritis, burns, diabetes, heart disease, strokes, spinal cord injury, and disabilities like Alzheimer’s and Parkinson’s disease. Currently hematopoietic stem cells are the only stem cells being used in the treatment. Human induced pluripotent stem cells may have a role in drug development for efficacy or toxicity screening; thus, eliminating the need for animal testing and shortening the time to commercialization. They open the window for personalized medical treatment with cell-based therapies based on stem cells that contain the genetic makeup or genetically engineered DNA of the patient.

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References 1. Becker, A.J., et al., Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells. Nature, 197, 452-454 (1963). 2. Evans, M.J., and Kaufman, M.H., Establishment in culture of pluripotential cells from mouse embryos. Nature, 292, 154-156 (1981).

3. Wilmut, I., et al., Viable offspring derived from fetal and adult mammalian cells. Nature, 385, 810-813 (1997). 4. Thompson, J.A., et al., Embryonic Stem Cell Lines Derived from Human Blastocysts. Science, 282, 1145-1147 (1998). 5. Gearhart, J., et al., Derivation of pluripotent stem cells from cultured human primordial germ cells. P.N.A.S., 95, 13726-13731 (1998). 6. Yamanaka, S., et al., Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 131, 861-872 (2007)

General Stem Cell Biology

The study of embryonic stem cells will continue to further our understanding of how they transform. Both private and government funding of embryonic stem cell research will be critical to reach this goal to provide a platform for the future. Human induced stems cells eliminate the need to use human embryos; thus, minimizing or even eliminating cultural, political, religious, and ethical concerns. The commercial and social impact to be gained by stem cell research coming to fruition is immeasurable. The ability to use stem cells instead of scalpels will affect how we practice medicine and approach personalized healthcare. As our understanding of genomics and proteomics has enriched our appreciation of the complexities of life, our exploration of stem cells will solve even more of its mysteries.

7. Thomson, J.A., et al., Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science, 318, 1917-1920 (2007). 8. French, A.J., et al., Development of Human cloned Blastocysts Following Somatic Cell Nuclear Transfer (SCNT) with Adult Fibroblast. Stem Cells published online Jan 17, 2008, http://stemcells. alphapharmedpress.org/cgi/reprint/2007-0252v1.pdf 9. Dr. Greg Wall, Ph.D., BioChemSultants, LLC

Protocols for Stem Cell Culture

Stem and feeder cell culture and cryopreservation 2x Freezing media preparation 20% fetal bovine serum (F2442) 20% DMSO (D2650) 60% ES cell media Store freezing media at -20 degrees C and thaw to 37 degrees C or room temperature before use. Ready-to-Go Freezing media Serum-free cyropreservation medium (C6295) Contains MEM, 8.7% DMSO, and methylcellulose DMSO-free cryopreservation medium (C6039) Contains MEM, glycerol, calf and fetal bovine serum Standard cryopreservation medium (C6164 Contains MEM, DMSO, calf and fetal bovine serum Complete media for mouse embryonic fibroblast (MEF) feeder cells

The information contained below is a compilation of various publications. For your convenience Sigma product numbers have been added where applicable, however these products have not been tested in stem cell applications.

Culture media formulation for ES (Embryonics Stem) and MEF Mouse Embryonic Fibroblast) feeder cells

For 500 ml of ES complete media: 100 ml fetal bovine serum (F2442) 5 ml 100x non-essential amino acids, NEAA (M7145) 5 ml 100 x Pen-Strep (P4333 or P0781) 5 ml 100 x sodium pyruvate (S8636 or P4562) 5 ml beta-mercaptoethanol Stock solution = 3.5 ul diluted to 5 ml neat beta-maerpactoethanol (M7522) with tissue culture grade water (W3500) - make fresh, do not store diluted solution 380 ml DME, high glucose (D5796) [See M3942 for reduced serum MegaCell formulation] 1 ml 106 Units/ml LIF (L5158) For positive and negative selections, add: 5 ml 100x G418 (G8169) 0.1 ml 5000x Ganciclovir ( use G2536 at 10 mM in PBS, P5368) Filter-sterilize as needed, store media at 4˚ C, pre-warm to 37 degrees C before use.

For 500 ml media: 445 ml DMEM (D5796) 50 ml fetal bovine serum (F2442) 5 ml 100x Pen-Strep (P4333 or P0781) Gelatin-treated culture ware All dishes/plates need to be treated with 0.2% gelatin for 5 minutes at room temperature. 0.2% gelatin is made from diluting 2% gelatin solution (G1393) with PBS (P5368) and autoclave to sterilize. The volume depends on culture vehicle, plates, slides, etc. It is necessary to cover the entire culture surface.

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General Stem Cell Biology

Stem and feeder cell culture and cryopreservation Expansion and irradiation of MEF feeder cells n Plate one vial primary MEF cells in one T75 flask with 20 ml MEF media. n Expand 1x T75 flask of cells into 1x T175 flasks. n Split 1x T175 flask of cells into 3 T175 flasks. n Split 3x T175 to 6xT175 flasks if more feeders are needed. n Trypsinize and pool all cells, spin down and resuspend cells in 50 ml MEF media. n Irradiate cells (gamma irradiation at 3000 rads). n Spin down and resuspend cells in MEF media. n Add an equal volume of 2x Freeze media. n Mix well and freeze for overnight in 0.5 ml aliquots at -80 degrees C. n Store in liquid nitrogen.

Freezing ES cells on 96-well plates Aspirate media and wash once with PBS. Freeze down the 3 x gelatinized only plate at -20 degrees C for DNA prep. For the 2 x plates with feeder cells, add 25 ul trypsin STV and incubate at 37 degrees C for 5 minutes. Disaggregate cells with 100 ul ES media and spin cells at 1K for 5 minutes. Take off supernatant. Be careful not to suck cells from bottom. Resuspend cells in 75 ul ES media.

Each T175 flasks of cells can be aliquoted into 6 vials. Each vial can then be plated onto 1x T25 flask. You may need to adjust these numbers according to plating efficiency. Routine maintenance of ES cells To maintain a healthy and undifferentiated culture, cells need to grow at high density (above 106 to 107/ 8cm2 or one well of a 6-well plate) and they should be passaged frequently. As a general rule, the media should be changed daily and cells should be passed every other day with a 5 fold split. Cells are oftens ‘shocked’ when coming out of freeze and may show a lag time from initial culture set up to the first passage, perhaps 3 to 4 days. Tips: Do a visual check of ES cultures. Observe for nesting (cells are in contact with each other). Undifferentiated ES cells should appear as compact colonies with prominent nucleoli. When cells are colonial or ‘nested’ individual cell borders should not be distinguishable but the colony should have a well defined boundary and may appear a little refractile under a phase microscope. To separate ES cells from MEF’s plate co-cultures on non-coated plates for 30 minutes at 37 degrees C. Feeder cells will attached leaving a relatively pure ES cell population in suspension.

Add 75 ul 2x freezing media. Cover plates with parafilm, place plates in plastic bags and then in Styrofoam box and put in -80 degrees C freezer. Thawing clones n Prepare feeder cells (50 ul /well) on 96-well plates. n Remove plates from -80 degrees C and let plates sit in warm bath. n After clones are thawed spin plates at 1K for 5 minutes. n Remove supernatant from well. n Resuspend clone in 100 ul ES media and add clone to fresh well with feeder cells. n After cells grow to confluence pass cells to 1 well of 24-well plate.

References 1. Stanford Transgenic Reesearch Center – online protocols 2. Nagy Lab, Samuel Lunenfeld, Mount Sinai Hospital – online protocols 3. Darwin Transgenic Mouse Core Facility, Baylor College of Medicine – online protocols 4. Comparative and Functional Genomics Dept., Max Planck Institute – online protocols

To pass ES cells: n Aspirate media off the plate and rinse once with PBS. n Trypsinize cells with 0.05% trypsin (T3924) at 37 degrees C for 3 to 5 minutes. n Add 5 ml of ES media and pipet vigorously to get single cell suspension. n Spin at 1000 rpm for 5 minutes. n Plate cells out onto irradiated feeder cells.

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Mouse ES cell freeze/thaw protocols

Stem and feeder cell culture and cryopreservation Many of the established hES cells have low recovery upon thawing, as low as 0.1 to 1%. This may be because of the method of passaging the cells. Mechanical picking or using collagenase dispersion usually results in large cell aggregates, which presumably do not get cryopreserved as efficiently as smaller clumps. Trypsinized cells in our lab have a recovery rate of about 10 to 20% or higher and do not require more complicated procedures such as vitrification (Figure 38-9). Freezing Medium The best recovery rate was observed in freezing medium consisting of 90% FBS-10% dimethyl sulfoxide (DMSO). However, Oct-4 expression in the thawed cells was lower than in cells frozen in hES cell growth medium with 10% DMSO. Nevertheless, by the next passage, the expression and distribution of Oct-4 and other markers of undifferentiated cells were indistinguishable between these two freezing conditions. We routinely use the 90% FBS-10% DMSO medium. Freezing Protocol Select a high-quality confluent culture with good morphology for freezing. We also recommend taking a picture of a sample field and staining for molecular markers characteristic of undifferentiated hES cells for future reference. Materials needed: n Chilled freezing medium: 90% FBS, 10% DMSO n Cryovials, labeled with the line, passage number, and date n Cryovial rack (rack with ice reservoir by Corning)(Sigma Cat. No. CLS430525) n Styrofoam rack from packaging for 15 ml centrifuge tubes n -80 °C freezer 1. Trypsinize the cells; centrifuge in PMEF medium (see earlier explanation). 2. Resuspend the pellet in the cold freezing medium. We recommend freezing one confluent 35 mm plate per vial in 0.5 ml of freezing medium. Work quickly and keep the cells on ice after the addition of the freezing medium. 3. Aliquot cell suspension into prechilled freezing vials and sandwich the vials between two Styrofoam racks: tape to prevent the two racks from separating and transfer to a –80 °C freezer overnight. Transfer the cryovials to liquid nitrogen for long-term storage.

Thawing hES Cells

Thawing hES cells is a relatively simple procedure. The main rule to follow is to do everything quickly.

30% hES cell- or PMEF-conditioned medium. 3. Change the medium on the PMEF plate to the thawing medium; equilibrate in the CO2 incubator for one hour. For 35 mm plates, use 1.5 ml medium; for four-well plates, use 0.5 ml medium per well. 4. Prepare a 50 ml conical tube with 10 to 15 ml of warm hES cell growth medium. Thawing 1. Thaw the vial in a 37 °C water bath, constantly agitating while ensuring that the neck of the vial is above the water level. Check the content of the vial after about 40 seconds and at 10-second intervals until only a small piece of ice remains. 2. Quickly spray the vial with 70% isopropanol, then using a 1 ml pipetteman*, add warm hES cell medium to the contents of the vial dropwise with gentle agitation. Do it quickly but very gently. Immediately transfer the contents into the prepared 50 ml tube with warm hES cell medium; centrifuge at 160 x g for 5 minutes. 3. Remove the medium completely without touching the pellet. 4. Add 0.5 ml of hES cell thawing medium, gently resuspend the cells using a 1 ml pipetteman (2 to 4 repetitions), and transfer to prepared PMEF plates with equilibrated hES cellthawing medium. Spread the cells evenly throughout the well by moving the plate several times in two directions at 90 degrees to each other; avoid swirling. 5. Check the cells the next day; if there are many dead cells or the medium has changed color, change two-thirds of the medium; otherwise, do not change it for another day. 6. The colonies usually begin to appear in 3 to 4 days and can be ready for splitting in 5 to 10 days (Figure 38-7).

General Stem Cell Biology

Freezing hES Cells

hES Cell Quality Control Although the morphology of hES cells is often used for evaluating the quality of the culture and its readiness for passaging or freezing, this criterion alone cannot be used for an assessment of ES cell pluripotency. Staining for the expression of Oct-4 or alkaline phosphatase even in colonies of "perfect" morphology can result in one or both of these markers appearing in the cells only at the periphery of the colony. It is important, therefore, to regularly assess the cells by analyzing the expression of markers of pluripotent cells. We look at Oct-4, SSEA-3, SSEA-4, TRA 1-60, and TRA 1-81 by immunostaining, or we perform an enzyme assay for alkaline phosphatase. The procedures for such assays and available antibodies are described elsewhere. *adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006

Preparation 1. Prepare mitomycin C-treated PMEFs a day before thawing. 2. Make a thawing medium. We use 70% hES cell growth medium supplemented with 2x hLIF and 8 ng/ml bFGF with

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Neuroscience Catalog The new catalog will present products useful in all areas of neuroscience research, including: n Agonists and Antagonists to Neurotransmitter n PCR/Amplification Reagents Receptors n Cloning Systems n Ion Channel Modulators n Transfection Reagents n Antibodies to Receptors, Ion Channels and n Antibody Arrays Transporters n Cell Culture Media, Supplements and Growth n Neuroproteins and Neuroenzymes Factors n Neurotoxins n Tools for Alzheimer’s Disease Research n Neuropeptides n Centrally Acting Drugs n RNAi Libraries

The 2008 - 2009 Sigma Life Science Catalog n 2000+ New Products – supporting Genomics, Functional Genomics, Cell Biology, Proteomics, and Metabolomics n New! Life Science Web Tool Guide – learn about the optimal online tool for finding the products and technical information you need n Introducing the Sigma BioUltra grade – setting the quality standard for Life Science research – High-purity, high-activity BioUltra proteins with complete documentation – Well-defined, high-purity BioUltra reagents with consistent certification testing n Over 34,000 Laboratory Reagents and Biochemicals to support your research – antibodies, antibiotics, cell culture media & reagents, enzymes, substrates, inhibitors, RNAi tools, Labware, and custom products

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Sigma-Aldrich Stem Cell Biology Platform!

Infinite Expansion, Infinite Potential! Sigma-Aldrich is pleased to announce the latest and most comprehensive selection of products for stem cell research! This platform is comprised of products necessary to the stem cell workflow from isolation and cryopreservation to in-vivo tracking with all of the related profiling tools in between! Please visit sigma-aldrich.com/stembio to view our complete portfolio! In addition to our product profiles you will also find the latest references, application notes, and a new Bulletin Board feature that will enable stem cell scientists like you to consult and exchange ideas and information! Our Innovation, Your Research — Shaping the Future of Life Science

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Our NEW Stem Cell Biology Platform begins with our comprehensive web site! Easily find products and detailed usage information in the following stem cell areas: N

Isolation

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Expansion

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Characterization

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Differentiation

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Functional Profiling

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In-vivo tracking and

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Much More!

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