General Stem Cell Biology
n Stem Cell Resources n Stem Cell Pathways n Stem Cell Markers
Infinite Expansion....Infinite Potential
n Stem Bio Overview n Protocols
Stem Cell Biology General Stem Cell Biology
Infinite Expansion....Infinite Potential Stem cells hold the key to a number of cellular processes from development, tissue regeneration, and aging. They also hold the promise of cures for many diseases and injuries as well as offer an opportunity to bridge the ‘animal to human gap’ in ADMET and drug efficacy studies, a necessary step in providing therapeutic products for human use. The potential of stem cells is certain, however bringing this potential to fruitition is contingent upon several key factors, not the least of them being the highly motivated and inteligent global research
Isolation
Characterization
Expansion
community. These scientists have and continue to contribute key incremental discoveries on nearly a daily basis and these discoveries move the entire science closer and closer to becoming the numerous miracle therapies that we have always been predicted. The stem cell biology platform, as presented in this brochure, highlights the most common general use media, supplements, reagents, and tools for stem cell discovery culture and analysis. Additional products can be found throughout the Sigma-Aldrich® catalog and website.
Differentiation
In vivo Tracking
Functional Profiling
Stem Cell Fate Germ Cells
Bone Marrow Blood
Mesoderm Bone Inner Cell Mass
Muscle Lung
ESC’s
Liver Intestine Endoderm Pancreas Stomach Esophagus Skin Ectoderm Neural Tissue
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Origins and Differentiation of Tissues Nervous tissue/NSCs Ectoderm Skin/skin SCs
Muscle and bone/tissue SC Lung, liver, and pancreas/organ specific SCs
Gastrulation
1
Endoderm
Esophagus, stomach, and intestine/intestinal SCs
Primordial germ cells
General Stem Cell Biology
Bone marrow and blood/HSCs and MSCs Mesoderm
Nervous tissue Ectoderm Skin Bone marrow and blood Mesoderm Muscle and bone Lung, liver, and pancreas
Gastrulation
2
Endoderm
Esophagus, stomach, and intestine
Primordial germ cells NSCs/skin SCs HSCs/MSCs/tissue SCs
Multipotent stem cells
Organ SCs/intestinal SCs
Putative developmental ontogeny of stem cells In lineage tree 1, the development of stem cells occurs after the formation of germ layers. These stem cells are thus restricted by germ layer commitment to their respective lineage (e.g., mesoderm is formed, giving rise to hematopoietic progenitors that become hematopoietic stem cells). Lineage tree 2 illustrates
the area that stem cells might develop similarly to PGCs, in that they avoid the lineage commitments during gastrulation and subsequently migrate to specific tissue and organ niches. (adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)
Our Innovation, Your Research
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Shaping the Future of Life Science
3
Genomic reprogramming in the oocyte, early embryos, and the germ line
General Stem Cell Biology
Specification of germ cells
Founder PGC (E7.5)
STELLA OCT-4 ESG-1 NANOG SOX2
ES cells
EG cells
Blastocyst (E7.5) Maternal inheritance & regulation of pluripotency EZH2 EED HP1
Migrating PGCs
DNA demethylation X re-activation erasure of imprints
STELLA OCT-4 ESG-1 Oocyte Initiation of imprinting
Epigenetic reprogramming Genital ridge E11.5
(adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)
Presenting! Prestige Antibodies™ Antibodies that work, right out of the box! The most highly characterized antibodies in the industry – 1,800 NEW Prestige Antibodies, powered by Atlas Antibodies ■
Designed & validated by the Human Proteome Resource (HPR)
■
Standardized in universal protocols
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Over 500 immunohistochemical images for every antibody
■
Publicly available data on the web
Look even closer. Go to sigma.com/prestige for more information. Our innovation, Your Research – Shaping the Future of Life Science Prestige Antibodies is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
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Product List
Workflow
Product Class
Isolation
Cryopreservation
C6164
Cell Freezing Medium-DMSO 1×
Isolation
Cryopreservation
C6295
Cell Freezing Medium-DMSO Serum free 1x
Isolation
Cryopreservation
C6039
Cell Freezing Medium-Glycerol 1×
Isolation
Cryopreservation
C2639
Cell Freezing Medium-Serum-free 1×
Isolation
Cryopreservation
D2650
Dimethyl sulfoxide
Isolation
Dissociation
E8008
EDTA solution
Isolation
Dissociation
E8140
Elastase from human leukocytes
Isolation
Enzymes
C2014
Collagenase, rat islet isolation
Isolation
Enzymes
C6079
Collagenase + Protease Inhibitors, suitable for islet isolation
Isolation
Enzymes
C9572
Collagenase, g-irradiated
Isolation
Enzymes
C9722
Collagenase, sterile, cell culture
Isolation
Enzymes
D4818
Dispase I
Isolation
Enzymes
D4693
Dispase II
Isolation
Isolation
a0561
ACCUSPIN™ System-Histopaque®-1077 Box of 100 tubes. Each 50 ml tube contains 15 ml Histopaque 1077-1 and will separate between 15 to 30 ml of anticoagulated blood.
Isolation
Isolation
A6929
ACCUSPIN System-Histopaque-1077 Box of 40 tubes. Each 12 ml tube contains 3 ml of Histopaque 1077-1 and will separate between 3 to 6 ml of anticoagulated blood.
Isolation
Isolation
A7054
ACCUSPIN System-Histopaque-1077 Box of 12 tubes. Each 50 ml tube contains 15 ml of Histopaque 1077-1 and will separate between 15 to 30 ml of anticoagulated blood.
Isolation
Isolation
F1418
Ficoll® 400 SigmaUltra, Type 400-DL, lyophilized powder
Isolation
Isolation
F2878
Ficoll PM 70 Type 70
Isolation
Isolation
F4375
Ficoll PM 400 Type 400, lyophilized powder
Isolation
Isolation
F5415
Ficoll solution Type 400, 20% in H2O
Isolation
Isolation
F8016
Ficoll 400 Type 400-DL, lyophilized powder, cell culture tested
Isolation
Isolation
F8636
Ficoll 400 lyophilized powder, γ-irradiated, cell culture tested
Isolation
Isolation
F9378
Ficoll 400 Type 400-DL, lyophilized powder
Isolation
Isolation
H8889
Histopaque-1077 Hybri-Max™ liquid, sterile-filtered, hybridoma tested
Isolation
Reagents
C6164
Cell Freezing Medium-DMSO
Characterization
Antibodies
A2726
Anti-ADAM-10, Cytoplasmic Domain antibody produced in rabbit
Characterization
Antibodies
A2851
Anti-ADAM-10, Prohormone Convertase Activation Site antibody produced in rabbit
Characterization
Antibodies
A2976
Anti-ADAM-12, N-terminal antibody produced in rabbit
Characterization
Antibodies
A3101
Anti-ADAM-10, Propeptide Domain antibody produced in rabbit
Characterization
Antibodies
A3226
Anti-ADAM-10, N-Terminal antibody produced in rabbit
Characterization
Antibodies
A3976
Anti-ADAM-17, Activation Site antibody produced in rabbit
Characterization
Antibodies
A4226
Anti-ADAM-17, Propeptide Domain antibody produced in rabbit
Characterization
Antibodies
A4351
Anti-ADAM-17, Cytoplasmic Domain antibody produced in rabbit
Characterization
Antibodies
A5102
Anti-Aurora B antibody produced in rabbit
Isolation
Product Number
Characterization
Product Name
Expansion
Our Innovation, Your Research
Differentiation
—
Functional Profiling
Shaping the Future of Life Science
General Stem Cell Biology
In some instances scientific milestones appear as serendipitous, nearly accidental discoveries. But for most of us discovery is a process and series of small steps that are the result of a long and wide pathway of techniques, methods, and in-depth analysis. In order to serve you better we have listed our the relevant products in the following pages by ‘work flow’ which is essentially addressing all of the steps in your discovery process. For every area of study, including regenerative medicine, there is a step-wise and systematic approach in gathering data. The proceeding products have been listed to appear in order of process: Isolation – products for enzymatic or gradient cell separation as well as storage/cryopreservation Characterization – product for cell identification Expansion – product to increase cell populations Differentiation – products to move cells forward along a given lineage and confirm lineage markers Functional profiling – products to assist in converting or diverting the intercellular processes, mechanisms, and pathways In vitro/in vivo analysis – products for tagging/marking cells in vitro and in vivo
In vivo Tracking
5
General Stem Cell Biology
Product List, cont. Workflow
Product Class
Characterization
Antibodies
A5228
Characterization
Antibodies
A5691
Anti-Smooth muscle alpha-actin - alkaline phosphatase, produced in mouse
Characterization
Antibodies
B3428
Anti-Bax antibody produced in rabbit
Characterization
Antibodies
B8429
Monoclonal Anti-Bax antibody produced in mouse, 2 mg/mL
Characterization
Antibodies
B9553
Monoclonal Anti-Bone Morphogenetic Protein 2
Characterization
Antibodies
C1366
Anti-CXCR3 antibody produced in rabbit
Characterization
Antibodies
C2542
Monoclonal Anti-N-Cadherin antibody produced in mouse
Characterization
Antibodies
C2667
Monoclonal Anti-N-Cadherin antibody produced in mouse
Characterization
Antibodies
C3116
Anti-CXCR4 antibody produced in rabbit
Characterization
Antibodies
C3366
Anti-CXCR5 antibody produced in rabbit
Characterization
Antibodies
C3865
Monoclonal Anti-N-Cadherin antibody produced in mouse
Characterization
Antibodies
C3960
Anti-Ciliary Neurotrophic Factor antibody, human, produced in goat
Characterization
Antibodies
C4085
Anti-Ciliary Neurotrophic Factor antibody, rat, produced in goat
Characterization
Antibodies
C4585
Monoclonal Anti-β-Tubulin−Cy3 antibody produced in mouse
Characterization
Antibodies
C5588
Anti-Cyclin D1 antibody produced in rabbit
Characterization
Antibodies
C6198
Anti-Smooth muscle alpha-actin - Cy3
Characterization
Antibodies
C7930
Anti-CD3, T Cell antibody produced in rabbit whole antiserum
Characterization
Antibodies
C8227
Anti-CXCR4 (Fusin), N-Terminal antibody produced in rabbit
Characterization
Antibodies
C8352
Anti-CXCR4 (Fusin) (182-196) antibody produced in rabbit
Characterization
Antibodies
C8354
Anti-CX3CR-1, N-Terminal antibody produced in rabbit
Characterization
Antibodies
C8479
Anti-CX3CR-1 (175-189) antibody produced in rabbit
Characterization
Antibodies
C9080
Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse
Characterization
Antibodies
D2442
Anti-Dopamine Transporter antibody produced in rabbit
Characterization
Antibodies
D4567
Anti-DNMT1, human, rat, mouse, antibody produced in rabbit
Characterization
Antibodies
D4692
Anti-DNMT1, human, mouse, antibody produced in rabbit
Characterization
Antibodies
D5567
Anti-dimethyl-Histone H3 (diMe-Lys9), hu, mo, rat, bovine, chicken, frog, Drosophila, C. elegans, A. thaliana produced in rabbit
Characterization
Antibodies
D5692
Anti-dimethyl-Histone H3 (diMe-Lys4) hu, mo, rat, bovine, chicken, frog, Drosophila, C. elegans, A. thaliana, produced in rabbit
Characterization
Antibodies
D9442
Anti-Dopamine Transporter, Extracellular Loop 2 antibody produced in rabbit
Characterization
Antibodies
D9567
Anti-Dopamine Transporter, C-Terminus antibody produced in rabbit
Characterization
Antibodies
E160
Anti-Eotaxin antibody produced in goat
Characterization
Antibodies
E1906
Anti-EAPII (TTRAP) antibody produced in rabbit
Characterization
Antibodies
F0300
Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit
Characterization
Antibodies
F0425
Anti-Fibroblast Growth Factor Receptor-3, Cytoplasmic antibody produced in rabbit
Characterization
Antibodies
F0550
Anti-Flt-3 antibody produced in goat
Characterization
Antibodies
F2043
Monoclonal Anti-β-Tubulin−FITC antibody produced in mouse
Characterization
Antibodies
F3304
Anti-Frizzled-2 antibody produced in rabbit
Characterization
Antibodies
F3393
Anti-Fibroblast Growth Factor-Basic (1-24) antibody produced in rabbit
Characterization
Antibodies
F3520
Anti-vonWillebrand Factor produced in rabbit
Characterization
Antibodies
F3648
Anti-Fibronectin antibody produced in rabbit
Characterization
Antibodies
F3777
Anti-Smooth muscle alpha-actin - FITC, produced in mouse
Characterization
Antibodies
F3804
Anti-Frizzled-1 antibody produced in rabbit
Characterization
Antibodies
F3922
Anti-Fibroblast Growth Factor Receptor-3, Extracellular antibody produced in rabbit
Characterization
Antibodies
F5421
Anti-Fibroblast Growth Factor Receptor-1 antibody produced in rabbit
Characterization
Antibodies
F5521
Anti-Fibroblast Growth Factor-Acidic antibody produced in rabbit
Isolation
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Characterization
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Product Number
Expansion
Product Name Anti-Smooth muscle actin, produced in mouse
Differentiation
TECHNICAL SERVICE: 800-325-5832
Functional Profiling
In vivo Tracking
Product Class
Characterization
Antibodies
F5537
Anti-Fibroblast Growth Factor-Basic antibody produced in rabbit
Characterization
Antibodies
F5900
Monoclonal Anti-Interleukin-2−FITC antibody produced in mouse, clone 5334.2
Characterization
Antibodies
F6796
Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit
Characterization
Antibodies
F8925
Anti-Flt-3/Flk-2 ligand (FL) antibody, rec mo
Characterization
Antibodies
F9049
Anti-Fibroblast Growth Factor-10 antibody produced in goat
Characterization
Antibodies
F9050
Anti-Flt-3/Flk-2 ligand (FL) antibody produced in goat
Characterization
Antibodies
F9304
Anti-FBI-1/Pokemon antibody produced in rabbit
Characterization
Antibodies
F9429
Anti-FBI-1/Pokemon (C-terminal) antibody produced in rabbit
Characterization
Antibodies
G0157
Anti-GABA Transporter GAT-1 antibody produced in rabbit
Characterization
Antibodies
G0290
Anti-GATA1 antibody produced in rabbit
Characterization
Antibodies
G0907
Anti-Granulocyte-Macrophage Colony Stimulating Factor
Characterization
Antibodies
G1544
Anti-GFP, N-terminal antibody produced in rabbit
Characterization
Antibodies
G4154
Anti-Granulocyte-Macrophage Colony Stimulating Factor Receptor α
Characterization
Antibodies
G4165
Anti-gp130 antibody produced in rabbit
Characterization
Antibodies
G4420
Anti-GRP94 (C-terminal) antibody produced in rabbit
Characterization
Antibodies
G4545
Anti-GRP94 antibody produced in rabbit
Characterization
Antibodies
G7657
Anti-GABA Transporter GAT-2 antibody produced in rabbit
Characterization
Antibodies
G8043
Anti-phospho-Growth Associated Protein-43 (pSer41) antibody produced in rabbit
Characterization
Antibodies
G8044
Anti-GATA-4 (pSer105) antibody produced in rabbit
Characterization
Antibodies
G8407
Anti-GABA Transporter GAT-3 antibody produced in rabbit
Characterization
Antibodies
G8918
Anti-GRP78/BiP (GL-19) antibody produced in rabbit
Characterization
Antibodies
G9043
Anti-GRP78/BiP (ET-21) antibody produced in rabbit
Characterization
Antibodies
G9152
Anti-Galactocerebroside antibody produced in rabbit whole antiserum
Characterization
Antibodies
G9269
Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit
Characterization
Antibodies
H0163
Monoclonal Anti-Histone Deacetylase 4 (HDAC4) antibody produced in mouse
Characterization
Antibodies
H0164
Anti-Histone H3 antibody produced in rabbit
Characterization
Antibodies
H0412
Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit
Characterization
Antibodies
H0788
Monoclonal Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in mouse
Characterization
Antibodies
H0913
Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in mouse
Characterization
Antibodies
H2039
Anti-HP1β antibody produced in rabbit
Characterization
Antibodies
H2164
Anti-HP1α antibody produced in rabbit
Characterization
Antibodies
H2287
Anti-Histone Deacetylase 6 (HDAC6) antibody produced in rabbit
Characterization
Antibodies
H2537
Anti-Histone Deacetylase 7 (HDAC7) (YS-19) antibody produced in rabbit
Characterization
Antibodies
H2662
Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit
Characterization
Antibodies
H2663
Monoclonal Anti-Histone Deacetylase 2 (HDAC2) antibody produced in mouse
Characterization
Antibodies
H2913
Monoclonal Anti-Histone Deacetylase 11 (HDAC11) antibody produced in mouse
Characterization
Antibodies
H3034
Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit
Characterization
Antibodies
H3159
Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit
Characterization
Antibodies
H4538
Monoclonal Anti-Histone Deacetylase 5 (HDAC5) antibody produced in mouse
Characterization
Antibodies
H5912
Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit
Characterization
Antibodies
H6287
Monoclonal Anti-Histone Deacetylase 1 (HDAC1) antibody produced in mouse
Characterization
Antibodies
H6409
Monoclonal Anti-phospho-Histone H3 (pSer10) antibody produced in mouse
Characterization
Antibodies
H6412
Monoclonal Anti-Histone Deacetylase 8 (HDAC8) antibody produced in mouse
Characterization
Antibodies
H6537
Monoclonal Anti-Histone Deacetylase 3 (HDAC3) antibody produced in mouse
Characterization
Antibodies
H6663
Monoclonal Anti-Histone Deacetylase 7 (HDAC7) antibody produced in mouse
Isolation
Product Number
Characterization
Product Name
Expansion
Our Innovation, Your Research
Differentiation
—
Functional Profiling
Shaping the Future of Life Science
General Stem Cell Biology
Workflow
In vivo Tracking
7
General Stem Cell Biology
Product List, cont. Workflow
Product Class
Characterization
Antibodies
H7162
Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit
Characterization
Antibodies
H7664
Anti-phospho-Histone H1.4 (pSer27) antibody produced in rabbit
Characterization
Antibodies
H7789
Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit
Characterization
Antibodies
H8038
Anti-Histone Deacetylase 8 (HDAC8) antibody produced in rabbit
Characterization
Antibodies
H8163
Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit
Characterization
Antibodies
H9286
Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit
Characterization
Antibodies
H9411
Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in rabbit
Characterization
Antibodies
H9536
Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in rabbit
Characterization
Antibodies
H9663
Anti-Histone Deacetylase 5 (HDAC5) (PI-16) antibody produced in rabbit
Characterization
Antibodies
H9908
Monoclonal Anti-phospho-Histone H3 (pSer28) antibody produced in rat
Characterization
Antibodies
I0505
Anti-IκBα antibody produced in rabbit
Characterization
Antibodies
I0649
Monoclonal Anti-Interleukin-6 Receptor antibody produced in mouse
Characterization
Antibodies
I1142
Monoclonal Anti-Interferon-γ antibody produced in rat
Characterization
Antibodies
I1404
Anti-Interleukin-1 Receptor Accessory Protein, C-Terminal antibody produced in rabbit
Characterization
Antibodies
I1644
Anti-Interleukin-5 antibody produced in goat
Characterization
Antibodies
I2143
Anti-Interleukin-6 antibody produced in rabbit
Characterization
Antibodies
I2407
Anti-Interleukin-3 Receptor α antibody produced in goat
Characterization
Antibodies
I2519
Monoclonal Anti-Interleukin-8 antibody produced in mouse
Characterization
Antibodies
I2532
Monoclonal Anti-Interleukin-7 antibody produced in mouse
Characterization
Antibodies
I2657
Anti-Interleukin-5 Receptor α antibody produced in goat
Characterization
Antibodies
I2782
Anti-Interleukin-7 antibody produced in goat
Characterization
Antibodies
I2907
Monoclonal Anti-Interleukin-7 Receptor α antibody produced in mouse
Characterization
Antibodies
I2908
Monoclonal Anti-Interferon-γ antibody produced in hamster
Characterization
Antibodies
I3392
Anti-Interleukin-1α antibody produced in goat
Characterization
Antibodies
I3393
Anti-Interleukin-6 antibody produced in rabbit
Characterization
Antibodies
I3642
Monoclonal Anti-Interleukin-1β antibody produced in mouse
Characterization
Antibodies
I3653
Anti-Interferon β antibody produced in goat
Characterization
Antibodies
I3767
Anti-Interleukin-1β antibody produced in goat
Characterization
Antibodies
I3778
Monoclonal Anti-Interleukin-1α antibody produced in mouse
Characterization
Antibodies
I4153
Anti-Interleukin-12 Receptor β1 antibody produced in goat
Characterization
Antibodies
I4902
Anti-Interferon-α/β Receptor 1 antibody produced in goat
Characterization
Antibodies
I5018
Anti-Interleukin-1α antibody produced in rabbit
Characterization
Antibodies
I5027
Anti-Interferon-γ (IFN-γ) antibody produced in goat
Characterization
Antibodies
I5152
Anti-Interferon-γ Receptor I (IFN-γ RI) produced in goat
Characterization
Antibodies
I5270
Anti-Interleukin-11 antibody produced in goat
Characterization
Antibodies
I5395
Anti-Interleukin-12 antibody produced in goat
Characterization
Antibodies
I5527
Monoclonal Anti-Interleukin-1 Soluble Receptor Type I antibody produced in mouse
Characterization
Antibodies
I5652
Monoclonal Anti-Interleukin-2 Soluble Receptor α antibody produced in mouse
Characterization
Antibodies
I5777
Monoclonal Anti-Interleukin-2 Soluble Receptor β antibody produced in mouse
Characterization
Antibodies
I5902
Monoclonal Anti-Interleukin-2 Soluble Receptor γ antibody produced in mouse
Characterization
Antibodies
I6152
Anti-Interleukin-2 Soluble Receptor α antibody produced in goat
Characterization
Antibodies
I6277
Anti-Interleukin-2 Soluble Receptor β antibody produced in goat
Characterization
Antibodies
I6402
Anti-Interleukin-2 Soluble Receptor γ antibody produced in goat
Characterization
Antibodies
I7401
Anti-Interleukin-2 antibody produced in goat
Characterization
Antibodies
I7642
Anti-Interleukin-12 antibody produced in goat
Isolation
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Characterization
ORDER: 800-325-3010
Product Number
Expansion
Product Name
Differentiation
TECHNICAL SERVICE: 800-325-5832
Functional Profiling
In vivo Tracking
Product Class
Characterization
Antibodies
I7901
Monoclonal Anti-Interleukin-6 antibody produced in mouse, clone 6708.11
Characterization
Antibodies
I8026
Anti-Interleukin-8 antibody produced in goat
Characterization
Antibodies
I8153
Anti-Interleukin-1 Receptor Accessory Protein antibody produced in rabbit
Characterization
Antibodies
I8273
Anti-Interleukin-2 antibody produced in goat
Characterization
Antibodies
I8398
Anti-Interleukin-2 antibody produced in goat
Characterization
Antibodies
I8638
Monoclonal Anti-Integrin β1 antibody produced in mouse
Characterization
Antibodies
I9016
Anti-Interferon-γ antibody produced in goat
Characterization
Antibodies
I9141
Anti-Interferon-γ (IFN-γ) antibody produced in goat
Characterization
Antibodies
I9898
Anti-Interleukin-5 antibody produced in goat
Characterization
Antibodies
IMMH2
Characterization
Antibodies
J3877
Anti-JAK3 antibody produced in rabbit
Characterization
Antibodies
J4127
Anti-Jagged 1 antibody produced in goat
Characterization
Antibodies
K4763
Anti-Ku70 antibody produced in rabbit
Characterization
Antibodies
K4888
Anti-Ku80 (XRCC5) (557-570) produced in rabbit
Characterization
Antibodies
L4418
Anti-LSD1 (AOF2) (C-terminal) antibody produced in rabbit
Characterization
Antibodies
L4793
Anti-LSD1 (AOF2) (N-terminal) antibody produced in rabbit
Characterization
Antibodies
L9152
Anti-Leukemia Inhibitory Factor antibody produced in goat
Characterization
Antibodies
L9277
Anti-Leukemia Inhibitory Factor antibody produced in goat
Characterization
Antibodies
M2420
Monoclonal Anti-Monocyte Chemotactic Protein-1 produced in mouse
Characterization
Antibodies
M254
Anti-Monocyte Chemotactic Protein 2 antibody produced in goat
Characterization
Antibodies
M255
Anti-Monocyte Chemotactic Protein 3 produced in goat
Characterization
Antibodies
M257
Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu
Characterization
Antibodies
M257
Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu.
Characterization
Antibodies
M4819
Anti-methyl-Histone H3 (Me-Lys4) antibody produced in rabbit
Characterization
Antibodies
M5917
Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. mo.
Characterization
Antibodies
M6042
Anti-Macrophage Inflammatory Protein-1α antibody produced in goat, rec. hu.
Characterization
Antibodies
M6792
Anti-Macrophage Inflammatory Protein-1β antibody produced in goat, rec. hu.
Characterization
Antibodies
M6917
Anti-Macrophage Inflammatory Protein-1α antibody produced in goat, rec.mo.
Characterization
Antibodies
M7273
Anti-Microtubule-Associated Proteins (MAPs) antibody produced in rabbit
Characterization
Antibodies
M7786
Anti-Smooth muscle myosin, heavy chain, produced in mouse
Characterization
Antibodies
N0649
Anti-Neuronal Specific Enolase antibody produced in rabbit
Characterization
Antibodies
N3038
Monoclonal Anti-Nanog produced in mouse
Characterization
Antibodies
N3279
Monoclonal Anti-Nerve Growth Factor-β antibody produced in mouse
Characterization
Antibodies
N3908
Anti-Nerve Growth Factor Receptor (NGFR p75) antibody produced in rabbit
Characterization
Antibodies
N4142
Anti-Neurofilament 200 antibody produced in rabbit
Characterization
Antibodies
N6655
Anti-Nerve Growth Factor 2.5S antibody produced in rabbit
Characterization
Antibodies
N6786
Monoclonal Anti-Notch1 antibody produced in mouse
Characterization
Antibodies
N8523
Monoclonal Anti-NF-κB antibody produced in mouse
Characterization
Antibodies
N8773
Anti-Nerve Growth Factor-β antibody produced in goat
Characterization
Antibodies
N9773
Anti-Neurotrophin-3 antibody produced in goat
Characterization
Antibodies
P0873
Anti-Oct4 produced in rabbit
Characterization
Antibodies
P2110
Anti-Platelet-Derived Growth Factor Receptor α antibody produced in goat
Characterization
Antibodies
P2229
Anti-Platelet-Derived Growth Factor Soluble Receptor β antibody produced in goat
Characterization
Antibodies
P5498
Anti-Pro-NGF antibody produced in rabbit
Characterization
Antibodies
P5976
Anti-Platelet-Derived Growth Factor-BB antibody produced in goat
Isolation
Product Number
Characterization
Product Name
General Stem Cell Biology
Workflow
Anti-Smooth Muscle Actin Immunohistology Kit
Expansion
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Differentiation
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General Stem Cell Biology
Product List, cont. Workflow
Product Class
Characterization
Antibodies
P6101
Anti-Platelet-Derived Growth Factor antibody produced in goat
Characterization
Antibodies
P6847
Monoclonal Anti-Interleukin-2−R-Phycoerythrin antibody produced in mouse
Characterization
Antibodies
R125
Anti-RANTES antibody produced in goat
Characterization
Antibodies
R2903
Anti-RALT/Mig-6 (SG-16) antibody
Characterization
Antibodies
R3028
Anti-RALT/MIG-6 (PE-16) antibody
Characterization
Antibodies
R5027
Anti-RIP140 antibody produced in rabbit
Characterization
Antibodies
R9528
Anti-RUNX1 antibody
Characterization
Antibodies
S192
Anti-SDF-1/PBSF antibody produced in goat
Characterization
Antibodies
S193
Anti-Synapsin I antibody produced in rabbit
Characterization
Antibodies
S2562
Anti-Stem Cell Factor Receptor (SCF R) antibody
Characterization
Antibodies
S2644
Anti-S-100 antibody produced in rabbit
Characterization
Antibodies
S2687
Anti-SDF-1β/PBSF antibody, recombinant human SDF-1β/PBSF expressed in E. coli, produced in goat, affinity isolated antibody
Characterization
Antibodies
S2822
Anti-Synapsin II antibody produced in rabbit
Characterization
Antibodies
S2947
Anti-Synapsin II (C-terminal) antibody produced in rabbit
Characterization
Antibodies
S4072
Anti-Sirt3 antibody produced in rabbit
Characterization
Antibodies
S4947
Anti-Sirt5 antibody produced in rabbit
Characterization
Antibodies
S5313
Anti-Sir2 (AS-16) antibody produced in rabbit
Characterization
Antibodies
S5947
Anti-Sirt7 antibody produced in rabbit
Characterization
Antibodies
S7693
Anti-Sox2 antibody produced in rabbit
Characterization
Antibodies
S7696
Anti-Sec23 antibody produced in rabbit
Characterization
Antibodies
S8316
Monoclonal Anti-SUV39H1 Histone Methyltransferase antibody produced in mouse ~2 mg/mL, clone 44.1, purified immunoglobulin, buffered aqueous solution
Characterization
Antibodies
T0198
Monoclonal Anti-β-Tubulin antibody produced in mouse, ~1 mg/mL, clone D66, purified immunoglobulin, buffered aqueous solution
Characterization
Antibodies
T0563
Anti-Transforming Growth Factor-α antibody, produced in goat
Characterization
Antibodies
T0688
Anti-Transforming Growth Factor-β1 antibody produced in chicken
Characterization
Antibodies
T0951
Anti-Tet Repressor (TETR) antibody produced in rabbit
Characterization
Antibodies
T1940
Anti-Transforming Growth Factor-β Soluble Receptor III antibody produced in goat
Characterization
Antibodies
T1941
Anti-TrkB antibody produced in goat
Characterization
Antibodies
T2200
Anti-β-Tubulin III antibody produced in rabbit
Characterization
Antibodies
T2565
Anti-Transforming Growth Factor-β Soluble Receptor II antibody produced in goat
Characterization
Antibodies
T2817
Anti-Tie 1 antibody produced in goat affinity isolated antibody
Characterization
Antibodies
T3192
Anti-Tie 2 antibody produced in goat
Characterization
Antibodies
T4026
Monoclonal Anti-β-Tubulin antibody produced in mouse, clone TUB 2.1, ascites fluid
Characterization
Antibodies
T4442
Anti-Transforming Growth Factor-β2 antibody produced in goat
Characterization
Antibodies
T4692
Anti-Transforming Growth Factor-β3 antibody produced in goat
Characterization
Antibodies
T4817
Anti-Transforming Growth Factor-β5 antibody produced in goat
Characterization
Antibodies
T4930
Anti-Thrombopoietin antibody produced in goat
Characterization
Antibodies
T5076
Monoclonal Anti-β-Tubulin Isotype III antibody produced in mouse, ~2 mg/mL, clone SDL.3D10, purified immunoglobulin, buffered aqueous solution
Characterization
Antibodies
T5201
Monoclonal Anti-β-Tubulin antibody produced in mouse, ~2 mg/mL, clone Tub2.1, purified immunoglobulin, buffered aqueous solution
Characterization
Antibodies
T5293
Monoclonal Anti-β-Tubulin antibody produced in mouse, clone 2-28-33, ascites fluid
Characterization
Antibodies
T5442
Anti-ADAM-17 (TACE), C-Terminal antibody produced in rabbit
Characterization
Antibodies
T7576
Anti-TGN46 antibody produced in rabbit
Characterization
Antibodies
T8660
Monoclonal Anti-β-Tubulin III antibody produced in mouse, clone SDL.3D10, ascites fluid
Characterization
Antibodies
T9429
Anti-Transforming Growth Factor-β, pan antibody produced in rabbit
Isolation
10
sigma-aldrich.com
Characterization
ORDER: 800-325-3010
Product Number
Expansion
Product Name
Differentiation
TECHNICAL SERVICE: 800-325-5832
Functional Profiling
In vivo Tracking
Product Class
Characterization
Antibodies
T9826
Anti-TGN38 antibody produced in rabbit
Characterization
Antibodies
U3254
Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat
Characterization
Antibodies
V1014
anti-Vascular Endothelial Growth Factor Receptor-2 from goat
Characterization
Antibodies
V1014
Anti-Flk1, Vascular Endothelial Growth Factor Receptor 2 antibody
Characterization
Antibodies
V1514
Anti-VE-Cadherin antibody produced in rabbit
Characterization
Antibodies
V2258
Monoclonal Anti-Vimentin antibody produced in mouse, clone LN-6, ascites fluid
Characterization
Antibodies
V4630
Anti-Vimentin antibody produced in goat, whole antiserum
Characterization
Antibodies
V5255
Monoclonal Anti-Vimentin antibody produced in mouse , clone VIM-13.2, ascites fluid
Characterization
Antibodies
V5764
Anti-Vesicular GABA Transporter antibody produced in rabbit
Characterization
Antibodies
V6389
Monoclonal Anti-Vimentin antibody produced in mouse, ~1 mg/mL, clone V9, purified immunoglobulin, buffered aqueous solution
Characterization
Antibodies
V6630
Monoclonal Anti-Vimentin antibody produced in mouse, clone V9, ascites fluid
Characterization
Antibodies
V7881
Monoclonal Anti-Vitronectin antibody produced in mouse
Characterization
Antibodies
W2141
Anti-Wnt-1 antibody produced in goat
Characterization
Antibodies
W2266
Anti-Wnt-4 antibody produced in goat
Characterization
Antibodies
W2516
Monoclonal Anti-Wnt-3a antibody produced in mouse
Characterization/Differentiation
Marker/Receptors
F8674
Fas/Fc Chimera human, recombinant, expressed in mouse NSO cells
Characterization/Differentiation
Marker/Receptors
F8799
Fas/Fc Chimera from mouse, recombinant, expressed in Sf21 cells
Characterization/Differentiation
Proteins/Peptides
A1229
Angiopoietin-3 from mouse recombinant, expressed in mouse NSO cells
Characterization/Differentiation
Proteins/Peptides
A1479
Angiopoietin-4 human recombinant, expressed in mouse NSO cells,
Characterization/Differentiation
Proteins/Peptides
A3156
Bovine Serum Albumin, globulin-free, g-irradiated
Characterization/Differentiation
Proteins/Peptides
A3782
Human Serum Albumin, fatty acid-free, 99%
Characterization/Differentiation
Proteins/Peptides
A4941
Activin A, recombinant, human
Characterization/Differentiation
Proteins/Peptides
A5226
Angiopoietin-1 human, recombinant, expressed in mouse NSO cells
Characterization/Differentiation
Proteins/Peptides
A5843
Human Serum Albumin, low endotoxin
Characterization/Differentiation
Proteins/Peptides
A6955
Angiogenin human, recombinant, expressed in Escherichia coli
Characterization/Differentiation
Proteins/Peptides
A7223
Human Serum Albumin, recombinant, from pichia pastoris
Characterization/Differentiation
Proteins/Peptides
A7979
Bovine Serum Albumin, 35% solution in DPBS
Characterization/Differentiation
Proteins/Peptides
A8806
Bovine Serum Albumin, fatty acid-free
Characterization/Differentiation
Proteins/Peptides
A9847
Angiopoietin-2 human, recombinant, expressed in mouse NSO cells
Characterization/Differentiation
Proteins/Peptides
A9975
ADAM-10 from mouse, recombinant, expressed in Sf21 cells
Characterization/Differentiation
Proteins/Peptides
B1182
Bcl-2 human recombinant, expressed in Escherichia coli
Characterization/Differentiation
Proteins/Peptides
B1436
BAX Inhibiting Peptide V5
Characterization/Differentiation
Proteins/Peptides
E2153
E-Cadherin/Fc Chimera from mouse
Characterization/Differentiation
Proteins/Peptides
H0786
Heregulin-β, EGF Domain human, recombinant, expressed in Escherichia coli
Characterization/Differentiation
Proteins/Peptides
H5529
Heregulin-α, EGF Domain human, recombinant, expressed in Escherichia coli
Characterization/Differentiation
Proteins/Peptides
H7660
Heregulin-β1 (EGF Domain) human, recombinant, expressed in Escherichia coli
Characterization/Differentiation
Proteins/Peptides
I3265
Interferon-γ human
Characterization/Differentiation
Proteins/Peptides
I3275
Interferon-γ from rat
Characterization/Differentiation
Proteins/Peptides
I4151
Interferon β 1a human
Characterization/Differentiation
Proteins/Peptides
I4276
Interferon-αA human
Characterization/Differentiation
Proteins/Peptides
I4401
Interferon-αA/D human
Characterization/Differentiation
Proteins/Peptides
I4777
Interferon-γ from mouse
Characterization/Differentiation
Proteins/Peptides
I4784
Interferon from human leukocytes
Characterization/Differentiation
Proteins/Peptides
I8654
Interferon-γ porcine
Characterization/Differentiation
Proteins/Peptides
I8657
Interferon α from rat
Isolation
Product Number
Characterization
Product Name
Expansion
Our Innovation, Your Research
Differentiation
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General Stem Cell Biology
Workflow
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General Stem Cell Biology
Product List, cont. Workflow
Product Class
Product Number
Product Name
Characterization/Differentiation
Proteins/Peptides
I8782
Interferon αA from mouse
Characterization/Differentiation
Proteins/Peptides
I8907
Interferon β from rat
Characterization/Differentiation
Proteins/Peptides
I9032
Interferon β from mouse
Characterization/Differentiation
Proteins/Peptides
n6784
Noggin, mouse recombinant
Characterization/Differentiation
Proteins/Peptides
N8911
Notch-3/Fc Chimera from mouse
Characterization/Differentiation
Proteins/Peptides
N9036
Notch-3/Fc Chimera human
Characterization/Differentiation
Proteins/Peptides
N9161
Notch-2/Fc Chimera from rat
Characterization/Differentiation
Proteins/Peptides
N9268
Notch-1/Fc Chimera from rat
Characterization/Differentiation
Proteins/Peptides
O8137
Osteoprotegerin/Fc Chimera from mouse
Characterization/Differentiation
Proteins/Peptides
O9631
Osteoprotegerin/Fc Chimera human
Expansion/Differentiation
Adhesion/Attachment/Matrices
C0543
Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane
Expansion/Differentiation
Adhesion/Attachment/Matrices
C1809
Collagen from kangaroo tail
Expansion/Differentiation
Adhesion/Attachment/Matrices
C3867
Collagen, Type I solution from rat tail Approx. 100mg/vial
Expansion/Differentiation
Adhesion/Attachment/Matrices
C5533
Collagen from human placenta
Expansion/Differentiation
Adhesion/Attachment/Matrices
C7661
Collagen I from rat tail
Expansion/Differentiation
Adhesion/Attachment/Matrices
C8919
Collagen from calf skin solution, sterile-filtered, 1 mg/mL
Expansion/Differentiation
Adhesion/Attachment/Matrices
C9301
Collagen from chicken sternal cartilage powder
Expansion/Differentiation
Adhesion/Attachment/Matrices
C9791
Collagen from calf skin powder
Expansion/Differentiation
Adhesion/Attachment/Matrices
E1270
ECM gel from Engelbreth-Holm-Swarm murine sarcoma
Expansion/Differentiation
Adhesion/Attachment/Matrices
E2153
E-Cadherin/Fc Chimera from mouse
Expansion/Differentiation
Adhesion/Attachment/Matrices
E2278
E-Cadherin/Fc Chimera human
Expansion/Differentiation
Adhesion/Attachment/Matrices
F0635
Fibronectin from rat plasma powder
Expansion/Differentiation
Adhesion/Attachment/Matrices
F0895
Fibronectin from human plasma 0.1% (Solution),
Expansion/Differentiation
Adhesion/Attachment/Matrices
F1141
Fibronectin from bovine plasma solution, 1 mg/mL
Expansion/Differentiation
Adhesion/Attachment/Matrices
F2006
Fibronectin from human plasma
Expansion/Differentiation
Adhesion/Attachment/Matrices
F2518
Fibronectin from human foreskin fibroblasts Cellular
Expansion/Differentiation
Adhesion/Attachment/Matrices
F4759
Fibronectin from bovine plasma lyophilized powder
Expansion/Differentiation
Adhesion/Attachment/Matrices
H4777
Heparan sulfate proteoglycan
Expansion/Differentiation
Adhesion/Attachment/Matrices
L2020
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
Expansion/Differentiation
Adhesion/Attachment/Matrices
L6274
Laminin from human placenta
Expansion/Differentiation
Adhesion/Attachment/Matrices
O2260
Osteopontin from mouse, recombinant, expressed in mouse NSO cells
Expansion/Differentiation
Adhesion/Attachment/Matrices
O3514
Osteopontin from bovine milk
Expansion/Differentiation
Adhesion/Attachment/Matrices
O4264
Osteopontin human recombinant, expressed in mouse NSO cells
Expansion/Differentiation
Adhesion/Attachment/Matrices
P5864
Proteoglycan from bovine nasal septum
Expansion/Differentiation
Adhesion/Attachment/Matrices
P5989
Proteoglycan from chicken sternal cartilage
Expansion/Differentiation
Adhesion/Attachment/Matrices
S7439
L-Selectin/Fc Chimera from mouse
Expansion/Differentiation
Adhesion/Attachment/Matrices
V0132
Vitronectin, from rat plasma
Expansion/Differentiation
Adhesion/Attachment/Matrices
V8379
Vitronectin from human plasma
Expansion/Differentiation
Adhesion/Attachment/Matrices
V9881
Vitronectin from bovine plasma
Expansion/Differentiation
Antibiotics
P4333
streptomycin/penicillin G, 100 ×, stabilized
Expansion/Differentiation
Antibiotics
G1272
Gentamicin solution, 10 mg/mL
Expansion/Differentiation
Antibiotics
P0781
Penicillin-Streptomycin, 100 ×
Expansion/Differentiation
Classical Media
D5796
DMEM
Expansion/Differentiation
Classical Media
D8062
DMEM
Expansion/Differentiation
Classical Media
D8537
DPBS without Ca and Mg
Isolation
12
sigma-aldrich.com
Characterization
ORDER: 800-325-3010
Expansion
Differentiation
TECHNICAL SERVICE: 800-325-5832
Functional Profiling
In vivo Tracking
Product Class
Expansion/Differentiation
Classical Media
I3390
IMDM
Expansion/Differentiation
Classical Media
M0644
MEM
Expansion/Differentiation
Classical Media
M4530
Med 199
Expansion/Differentiation
Classical Media
M4655
MEM
Expansion/Differentiation
Classical Media
M8042
MEM
Expansion/Differentiation
Classical Media
R8758
RPMI
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
B1434
Bone Morphogenetic Protein 7 human, recombinant, expressed in CHO cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
B2680
BMP-4
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
B3555
Bone Morphogenetic Protein 2 (BMP-2) human, recombinant, expressed in Escherichia coli, cell culture tested
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
B3795
Brain-derived neurotrophic factor human recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
C3710
Ciliary Neurotrophic Factor human, ≥97% (SDS-PAGE), recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
C3835
Ciliary Neurotrophic Factor from rat, ≥98% (SDS-PAGE and HPLC), recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
C8615
CXCL16 human recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
E1257
Epidermal Growth Factor from murine submaxillary gland, cell culture tested
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
E1388
β-Endothelial Cell Growth Factor human recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
E4127
Epidermal Growth Factor from murine submaxillary gland
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
E4269
LONG™ EGF human, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
E4643
Heparin-Binding EGF-Like Growth Factor human recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
E8399
Eotaxin-3 human, recombinant, expressed in Escherichia coli,
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
e8403
Eotaxin−Biotin from mouse
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
E9152
Eotaxin-2 from mouse, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
E9644
Epidermal Growth Factor human recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F0291
Fibroblast Growth Factor-Basic human (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F1168
Fibroblast Growth Factor-9 human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F1802
Fibroblast Growth Factor-8c from mouse
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F1927
Fibroblast Growth Factor Receptor 2β (IIIc)/Fc Chimera from mouse
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F2052
Fibroblast Growth Factor Receptor 2β (IIIb)/Fc Chimera from mouse
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
f3133
Fibroblast Growth Factor from bovine pituitary
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F3422
Flt-3/Flk-2 ligand human, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F4537
Fibroblast Growth Factor-5 human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F4662
Fibroblast Growth Factor-6 human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F5392
Fibroblast Growth Factor-Basic from bovine pituitary (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F5542
Fibroblast Growth Factor-Acidic human
Isolation
Product Number
Characterization
Product Name
Expansion
Our Innovation, Your Research
Differentiation
—
Functional Profiling
Shaping the Future of Life Science
General Stem Cell Biology
Workflow
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General Stem Cell Biology
Product List, cont. Workflow
Product Class
Product Number
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F5895
Fibroblast Growth Factor Basic Fragment 1-24 bovine (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F6926
Fibroblast Growth Factor-8b from mouse
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F7176
Fibroblast Growth Factor-17 human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F7301
Fibroblast Growth Factor-18 human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F8424
Fibroblast Growth Factor-4 human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F8675
Fibroblast Growth Factor Receptor-4/Fc Chimera human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F8924
Fibroblast Growth Factor 10 human, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F9174
Fibroblast Growth Factor Receptor-1α (IIIc)/Fc Chimera human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F9175
Flt-3/Flk-2 Ligand from mouse (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F9299
Fibroblast Growth Factor Receptor-1β (IIIc)/Fc Chimera human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F9424
Fibroblast Growth Factor Receptor-2α (IIIb)/Fc Chimera human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F9549
Fibroblast Growth Factor Receptor-2α (IIIc)/Fc Chimera human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F9647
Fibroblast Growth Factor Receptor 2β (IIIc)/Fc Chimera from mouse
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F9786
Fibroblast Growth Factor-Basic Heparin Stabilized human (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F9799
Fibroblast Growth Factor Receptor-2β (IIIc)/Fc Chimera human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
F9924
Fibroblast Growth Factor Receptor-3α (IIIc)/Fc Chimera from mouse
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
G0282
Granulocyte-Macrophage Colony-Stimulating Factor from mouse, recombinant, expressed in Escherichia coli, lyophilized powder, ≥97% (SDS-PAGE), cell culture tested (Sigma)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
G0407
Granulocyte Colony-Stimulating Factor
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
G0792
Granulocyte-Macrophage Colony-Stimulating Factor from rat
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
G5035
Granulocyte-Macrophage Colony-Stimulating Factor from human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
G7545
gp130 Soluble Fragment human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
G8160
Granulocyte Colony-Stimulating Factor
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
H1404
HGF, recombinant, human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
H9661
HGF, recombinant, human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I0406
Interleukin-6 from rat recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I0523
Interleukin-2 from mouse, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I0779
Interleukin-2 Soluble Receptor α human, recombinant, expressed in mouse NSO cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I0904
Interleukin-2 Soluble Receptor β human, recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I1029
Interleukin-2 Soluble Receptor γ human, recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I1145
Interleukin-5 from mouse, recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I1271
LONG™ R3 IGF-1, Recombinant Analog human (1)
Isolation
14
sigma-aldrich.com
Characterization
ORDER: 800-325-3010
Expansion
Product Name
Differentiation
TECHNICAL SERVICE: 800-325-5832
Functional Profiling
In vivo Tracking
Product Class
Product Number
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I1395
Interleukin-6 human recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I1645
Interleukin-8 human, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I1646
Interleukin-3 human recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I2276
Interleukin-12 human, recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I2393
Interleukin-1β from rat, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I2406
Interleukin-11 human, recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I2526
Insulin-like Growth Factor-II human (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I2644
Interleukin-2 human, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
i2656
Insulin-like Growth Factor-I (E3R) human (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I2778
Interleukin-1α human, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I3263
Insulin-like Growth Factor-II fragment 33-40 (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I3268
Interleukin-6 human from human (osteosarcoma cells),
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I3657
Insulin-Like Growth Factor Binding Protein-2 from mouse (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I3769
IGF-1, recombinant, human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I3901
Interleukin-1α from rat, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I4144
Interleukin-3 from mouse recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I4638
Interleukin-1β Fragment 163-171 human, synthetic
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I4892
Interleukin-7 from mouse, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I5271
Interleukin-1β from mouse, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I5273
Interleukin-5 human, recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I5277
Interferon-γ Receptor 1 human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I5396
Interleukin-1α from mouse, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I5403
Insulin-Like Growth Factor Binding Protein-2 human (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I5771
Interleukin-6 Receptor Soluble Fragment human recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I5896
Interleukin-7 human, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I7389
Interleukin-3 human recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I7908
Interleukin-2 human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I8148
Interleukin-1 Soluble Receptor, Type II human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I8523
Interleukin-12 from mouse, recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I8779
Insulin-like Growth Factor-I from mouse (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I8904
Insulin-like Growth Factor-II from mouse (1)
Isolation
Characterization
Product Name
Expansion
Our Innovation, Your Research
Differentiation
—
Functional Profiling
Shaping the Future of Life Science
General Stem Cell Biology
Workflow
In vivo Tracking
15
General Stem Cell Biology
Product List, cont. Workflow
Product Class
Product Number
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I9279
Interleukin-11 from mouse, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I9401
Interleukin-1β human, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I9646
Interleukin-6 from mouse recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I9780
Interleukin-2/Fc Chimera (non-lytic) human, recombinant, expressed in NS.1 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
I9905
Interleukin-2/Fc Chimera (non-lytic) from mouse, recombinant, expressed in CHO cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
L3408
Transforming Growth Factor-β1 Latency Associated Peptide human, recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
L5158
LIF (Leukemia Inhibitory Factor), recombinant, mouse
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
L5283
LIF (Leukemia Inhibitory Factor), recombinant, human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
M1439
MIG from mouse
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
M252
MIG human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
M261
Platelet-Derived Growth Factor-BB from rat
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
M6167
Macrophage Inflammatory Protein-1α from mouse, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
M6292
Macrophage Inflammatory Protein-1α human, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
M6417
Macrophage Inflammatory Protein-1β human, recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
M6542
Macrophage Inflammatory Protein-1β from mouse, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
N0513
Nerve Growth Factor-7S from murine submaxillary gland
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
N1408
Nerve Growth Factor-β human recombinant
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
N1780
Neurotrophin-4 human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
N1905
Neurotrophin-3 human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
N2513
Nerve Growth Factor-β from rat recombinant
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
N6009
Nerve Growth Factor-2.5S from murine submaxillary gland
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
N8133
Nerve Growth Factor from Vipera lebetina venom
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
N8898
Nerve Growth Factor Receptor/Fc Chimera human recombinant
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
N9411
Nerve growth factor receptor/Fc chimera from mouse recombinant
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
P1985
Platelet-Derived Growth Factor Soluble Receptor α human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
P3076
Platelet-Derived Growth Factor-AA human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
P3201
Platelet-Derived Growth Factor-BB human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
P3326
Platelet-Derived Growth Factor-AB human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
P3618
Platelet-Derived Growth Factor Receptor β/Fc Chimera human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
P4056
Platelet-Derived Growth Factor-BB from rat
Isolation
16
sigma-aldrich.com
Characterization
ORDER: 800-325-3010
Expansion
Product Name
Differentiation
TECHNICAL SERVICE: 800-325-5832
Functional Profiling
In vivo Tracking
Product Class
Product Number
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
P4306
Platelet-Derived Growth Factor-BB human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
P8147
Platelet-Derived Growth Factor from human platelets
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
P8953
Platelet-Derived Growth Factor from porcine
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
R2274
RANTES from mouse
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
R6267
RANTES human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
S190
Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor human, recombinant, expressed in Escherichia coli,
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
S2437
SCF Soluble Receptor human
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
S5816
Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor from mouse recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
S7901
Stem Cell Factor human recombinant
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
S8406
Stromal Cell-Derived Factor 1β/pre-B Cell Growth Stimulating Factor human, recombinant, expressed in Escherichia coli,
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
S9915
Stem Cell Factor from mouse recombinant
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T0892
Interleukin-2 from rat spleenocytes
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T1566
Tie-1/Fc Chimera human, recombinant, expressed in mouse NSO cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T1654
Transforming Growth Factor-β1 from human platelets
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T2815
Transforming Growth Factor-β2 human, recombinant, expressed in NSO cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T2926
Transforming Growth Factor-β5 amphibian, recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T3448
Tie-2/Fc Chimera from mouse, recombinant, expressed in mouse NSO cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T3698
Transforming Growth Factor-β Receptor II/Fc Chimera from mouse, recombinant, expressed in mouse NSO cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T4567
Transforming Growth Factor-β Soluble Receptor III human, recombinant, expressed in mouse NSO cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T5050
Transforming Growth Factor-β1 from porcine platelets
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T5278
Transforming Growth Factor-α from rat, g-irradiated
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T5300
Transforming Growth Factor-β2 from porcine platelets
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T5425
Transforming Growth Factor-β3 human, recombinant, expressed in Sf21 cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T7039
Transforming Growth Factor-β1 human, recombinant, expressed in insect cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T7039
Transforming Growth Factor-β1 human, recombinant, expressed in insect cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T7924
Transforming Growth Factor-α human, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T8569
TrkC/Fc Chimera human recombinant, expressed in mouse NSO cells
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T8694
TrkB/Fc Chimera human recombinant, expressed in mouse NSO cells,
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
T9533
Transforming Growth Factor-α from rat
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
V1260
VEGF Receptor-3 (Flt-4)/Fc Chimera human
Isolation
Characterization
Product Name
Expansion
Our Innovation, Your Research
Differentiation
—
Functional Profiling
Shaping the Future of Life Science
General Stem Cell Biology
Workflow
In vivo Tracking
17
General Stem Cell Biology
Product List, cont. Workflow
Product Class
Product Number
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
V3388
Vascular Endothelial Growth Factor 121 human (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
V3513
Vascular Endothelial Growth Factor 120 from mouse (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
V3638
Vascular Endothelial Growth Factor 164 from rat (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
V4512
Vascular Endothelial Growth Factor from mouse (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
V6012
Vascular Endothelial Growth Factor D human (1)
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
V6633
VEGF Receptor-3 (Flt-4)/Fc Chimera from mouse
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
V6758
VEGF Receptor-2(Flk-1, KDR)/Fc ChimeraVEGF Receptor-2
Expansion/Differentiation
Cyokines/Growth Factors/ Chemokines
V7259
Vascular Endothelial Growth Factor human (1)
Expansion/Differentiation
Enzymes
F2803
Flt-3/Fc Chimera, rec mo
Expansion/Differentiation
Enzymes
F7426
Flt-3/Fc Chimera human, recombinant, expressed in mouse NSO cells,
Expansion/Differentiation
Enzymes
L0164
LYN B human, recombinant, expressed in insect cells
Expansion/Differentiation
Enzymes
L9913
LYN A human, recombinant, expressed in insect cells
Expansion/Differentiation
Specialty Media
51800C
Expansion/Differentiation
Stemline Media
S0189
Stemline HSC
Expansion/Differentiation
Stemline Media
S0192
Stemline HSC
Expansion/Differentiation
Stemline Media
S0196
Stemline Keratinocyte
Expansion/Differentiation
Stemline Media
S1569
Stemline MSC
Expansion/Differentiation
Stemline Media
S1694
Stemline T cell
Expansion/Differentiation
Stemline Media
S3194
Stemline Neural Stem Cell Expansion medium
Expansion/Differentiation
Stemline Media
S3444
Stemline DC
Expansion/Differentiation
Stemline Media
S9945
Stemline Keratinocyte Medium Supplement with BPE
Expansion/Differentiation
Vitamins, Hormones, Misc.
A4034
sodium ascorbate (Takahashi, 2003)
Expansion/Differentiation
Vitamins, Hormones, Misc.
A6791
Atrial Natriuretic Peptide fragment 3-28 rat
Expansion/Differentiation
Vitamins, Hormones, Misc.
D4902
Dexamethasone, cell culture tested
Expansion/Differentiation
Vitamins, Hormones, Misc.
D8893
Dexamethasone, γ-irradiated, cell culture tested
Expansion/Differentiation
Vitamins, Hormones, Misc.
R0635
Retinol Acetate - soluble
Expansion/Differentiation
Vitamins, Hormones, Misc.
R2625
retinoic acid
Expansion/Differentiation
Vitamins, Hormones, Misc.
R7882
Retinol Acetate
Expansion/Differentiation
Vitamins, Hormones, Misc.
T1568
Thrombopoietin human, recombinant, expressed in Escherichia coli
Expansion/Differentiation
Vitamins, Hormones, Misc.
T4184
Thrombopoietin from mouse, recombinant, expressed in NSO cells
Functional Profiling
Inhibitors
P4484
Purvalanol A
Functional Profiling
Ion Channels
17783
Calcein-AM
Functional Profiling
Kit
BLOT2
Northern Blots, poly(A)+ RNA from BALB/c mouse, multiple tissue
Functional Profiling
Kit
BLOT3
Northern Blots, poly(A)+ RNA from BALB/c murine embryo
Functional Profiling
Kit
BPQ24
BioPORTER® QuikEase™ Protein Delivery Kit
Functional Profiling
Kit
BPQ96
BioPORTER QuikEase Protein Delivery Kit
Functional Profiling
Kit
CSAA1
Panorama® Antibody Microarray - Cell Signaling Kit
Functional Profiling
Kit
D7440
JumpStart™ Taq ReadyMix™ for Quantitative PCR
Functional Profiling
Kit
DMN10
GenElute™ Direct mRNA Miniprep Kit sufficient for 10 purifications
Functional Profiling
Kit
DNAQF
DNA Quantitation Kit, Fluorescence Assay
Functional Profiling
Kit
G1N10
GenElute Mammalian Genomic DNA Miniprep Kit sufficient for 10 purifications
Functional Profiling
Kit
GRAA2
Panorama Antibody Microarray - Gene Regulation I Kit
Isolation
18
sigma-aldrich.com
Characterization
ORDER: 800-325-3010
Expansion
Product Name
Claycomb medium
Differentiation
TECHNICAL SERVICE: 800-325-5832
Functional Profiling
In vivo Tracking
Workflow
Product Class
Functional Profiling
Kit
HPFM4
Functional Profiling
Kit
ID0010
In vitro Director™ PCR System 3′ FLAG®
Functional Profiling
Kit
ID0020
In vitro Director PCR System 5′ c-Myc
Functional Profiling
Kit
ID0030
In vitro Director PCR System 5′ FLAG
Functional Profiling
Kit
ID0040
In vitro Director PCR System 5′ Anchor™
Functional Profiling
Kit
MRN10
GenElute mRNA Miniprep Kit sufficient for 10 purifications
Functional Profiling
Kit
N5659
Northern Bound™ Human Alzheimer’s Total RNA Blot II from human
Functional Profiling
Kit
N5784
Northern Bound Human mRNA Blot
Functional Profiling
Kit
N5909
Northern Bound Human mRNA Blot II
Functional Profiling
Kit
N7409
Northern Bound Human Normal Brain Total RNA Blot
Functional Profiling
Kit
N7534
Northern Bound Human Normal Brain Total RNA Blot II
Functional Profiling
Kit
NA2000
Functional Profiling
Kit
PPAA4
Panorama Antibody Array -p53 pathways
Functional Profiling
Kit
R2653
RapidTransit™ Transformation Kit
Functional Profiling
Kit
RTN10
GenElute Mammalian Total RNA Miniprep Kit sufficient for 10 purifications
Functional Profiling
Kit
SH0211
MISSION® shRNA Human G-Protein Coupled Receptors, Glycerol Set-1
Functional Profiling
Kit
SH0231
MISSION shRNA Human G-Protein Coupled Receptors, Lentiviral Transduction Particle Set 1
Functional Profiling
Kit
SH0511
MISSION shRNA Human Tumor Suppressors: Glycerol Set-1
Functional Profiling
Kit
SH0531
MISSION shRNA Human Tumor Suppressors: Lentiviral Transduction Particle Set
Functional Profiling
Kit
SH0831
MISSION shRNA Human Cytokines and Chemokines, Lentiviral Transduction Particle Set 1
Functional Profiling
Kit
SH1011
MISSION shRNA Human Ion Channels: Glycerol Set-1
Functional Profiling
Kit
SH1031
MISSION shRNA Human Ion Channels, Lentiviral Transduction Particle Set 1
Functional Profiling
Kit
SH1311
MISSION shRNA Human Cytokine and Chemokine Receptors, Glycerol Set-1
Functional Profiling
Kit
WGA1
GenomePlex® Whole Genome Amplification (WGA) Kit
Functional Profiling
Kit
WGA4
GenomePlex Single Cell Whole Genome Amplification Kit
Functional Profiling
Kit
WTA1
TransPlex® Whole Transcriptome Amplification Kit
Functional Profiling
Kit
XNAB2
Extract-N-Amp™ Blood PCR Kit sufficient for 100 extractions, sufficient for 100 amplifications
Functional Profiling
Kit
XNABRE
REDExtract-N-Amp™ Blood PCR Kit sufficient for 10 extractions, sufficient for 10 amplifications
Functional Profiling
Reagent
D5758
Diethyl pyrocarbonate ≥97% (NMR)
Functional Profiling
Transfection/Transduction
N2913
N-TER Nanoparticle siRNA Transfection System
Functional Profiling
Transfection/Transduction
NPT01
NeuroPorter™ Transfection Kit
Functional Profiling
Transfection/Transduction
SHC002
Functional Profiling
Transfection/Transduction
SHC002V
Functional Profiling
Transfection/Transduction
SHC003
Functional Profiling
Transfection/Transduction
SHC003V
In vitro & In vivo Tracking
Stains/Syes
32857
Wright Stain solution
In vitro & In vivo Tracking
Stains/Syes
D8417
DAPI
In vitro & In vivo Tracking
Stains/Syes
G9641
Giemsa stain
In vitro & In vivo Tracking
Stains/Syes
HT90T
Gram stain for tissue
In vitro & In vivo Tracking
Stains/Syes
M5655
MTT
In vitro & In vivo Tracking
Stains/Syes
P1951
Phalloidin–Tetramethylrhodamine B isothiocyanate Phalloidin from Amanita phalloides
In vitro & In vivo Tracking
Stains/Syes
P5282
Phalloidin, Fluorescein Isothiocyanate Labeled Phalloidin from Amanita phalloides
In vitro & In vivo Tracking
Stains/Syes
T8154
Trypan Blue solution 0.4%, liquid, sterile-filtered, cell culture tested
In vitro & In vivo Tracking
Stains/Syes
X4626
XTT
Characterization
Product Name Panorama® Human Protein Function Array -Signal Transduction
General Stem Cell Biology
Isolation
Product Number
GenElute Blood Genomic DNA Kit sufficient for 10 purifications
MISSION Non-Target shRNA Control Transduction Particles MISSION Non-Target shRNA Control Vector MISSION TurboGFP™ Control Vector MISSION TurboGFP Control Transduction Particles
Expansion
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Differentiation
—
Functional Profiling
Shaping the Future of Life Science
In vivo Tracking
19
General Stem Cell Biology
Stem Cell Marker Table
20
Category
Marker
ASC
Adipose-derived mesenchymal stem cell
ASC, PSC
CXCR4
ASC, PSC
ATP Binding Cassette G2
ASC, PSC
Gene
PN
chemokine cell surface receptor
CXCR4
C8227
Anti-CXCR4 (Fusin), N-Terminal
transporter protein
ABCG2
B7165
Anti-Breast Cancer Resistance Protein
ATP Binding Cassette G2
transporter protein
ABCG2
B7059
Monoclonal Anti-Breast Cancer Resistance Protein
ASC, PSC
ATP Binding Cassette G2
transporter protein
ABCG2
B7684
Monoclonal Anti-Breast Cancer Resistance Protein
AGPC
Glial Fibrillary Acidic Protein
Intermediate filament protein
GFAP
G9269
Anti-Glial Fibrillary Acidic Protein
AGPC
Glial Fibrillary Acidic Protein
Intermediate filament protein
GFAP
G3893
Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP)
BSC
Osteoblast mesenchymal stem cell, periosteum progenitor
CM
GATA-4
GATA4
G8044
Anti-phospho-GATA4 (pSer105) antibody produced in rabbit
CM
Cardiac troponin I
EB
Embryoid body
EB, HSC, CM
Flk1
Cell surface receptor
KDR
V1014
Anti-Vascular Endothelial Growth Factor Receptor 2
EB, HSC, CM
Flk1
Cell surface receptor
KDR
V9134
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2
EB, HSC, CM
Flk1
Cell surface receptor
KDR
V3003
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-2 (KDR)
EGC
Embryonic germ cell
Epithelial SC
Cytokeratin 19
KRT19
C6930
Monoclonal Anti-Cytokeratin Peptide 19
Epithelial SC
p63
Trp63
P3362
Monoclonal Anti-p63
Epithelial SC
p63
Trp63
P3737
Monoclonal Anti-p63
ERC
Endometrial Regenerative Cell
ERC, BSC, MSC, ASC, SMPC
Endoglin (CD105)
TGFbeta coreceptor
ESC
SSEA-3 (stage-specific embryonic antigen)
glycolipid antigen
ESC
SSEA-4 (state-specific embryonic antigen)
glycolipid antigen
ESC
GCT343
karatan sulfate antigen
ESC
GCTM2
karatan sulfate antigen
ESC
Embryonic stem cell
ESC, ASC, ERC, BSC, GSC
CD9
Cell adhesion, motility
CD9
ESC, HSC, MSC, ERC, GSC
Thy-1 (CD90)
GPI-anchored cell surface protein, adhesion
THY1
M7898
Monoclonal Anti-Thy 1.1
ESC, MSC, BSC
tissue nonspecific alkaline phosphatase
ESC, PSC
Rex-1
zinc finger protein
Zfp42
GSC
Integrin alpha 6
Cell adhesion
ITGA6
GSC
Stra8
GSC
Germline stem cell
HSC
CD38
Cell surface receptor, enzyme
C1586
Monoclonal Anti-CD38
HSC
CD45
Cell surface receptor, protein phosphatase
PTPRC
C7556
Monoclonal Anti-CD45
HSC
CD34
Cell surface/cell adhesion
CD34
HSC
c-Kit (CD117 antigen)
Stem Cell Factor receptor
KIT
K0131
Monoclonal Anti-c-kit/SCF Receptor antibody
HSC
c-Kit (CD117 antigen)
Stem Cell Factor receptor
KIT
C6740
Anti-phospho-c-Kit (SCFR) (pTyr703) antibody produced in rabbit
HSC
c-Kit (CD117 antigen)
Stem Cell Factor receptor
KIT
C6615
Anti-phospho-c-Kit (SCFR) (pTyr730) antibody produced in rabbit (1)
HSC
c-Kit (CD117 antigen)
Stem Cell Factor receptor
KIT
C6490
Anti-phospho-c-Kit (SCFR) (pTyr823) antibody produced in rabbit (1)
HSC
c-Kit (CD117 antigen)
Stem Cell Factor receptor
KIT
C6365
Anti-phospho-c-Kit (SCFR) (pTyr936) antibody
HSC
Sca-1
HSC
Hematopoietic stem cell
LSC
CD96
sigma-aldrich.com
ORDER: 800-325-3010
Function/Pathway
Cardiomyocyte differentiation through BMP
SA Antibody
TNNI3
Epithelial intermediate filament protein
ENG
alpl
STRA8
CD96
TECHNICAL SERVICE: 800-325-5832
Marker
Function/Pathway
Gene
PN
LSC
Cytokeratin 18
Epithelial intermediate filament protein
Krt18
C1399
Monoclonal Anti-Cytokeratin Peptide 18
LSC
Cytokeratin 18
Epithelial intermediate filament protein
Krt18
C8541
Monoclonal Anti-Cytokeratin Peptide 18
LSC
Hepatic/biliary stem cells
MPC
Desmin
cytoskeletal protein
SA Antibody
DES
D8281
Anti-Desmin
H1650
Monoclonal Anti-HLA Class I Antigen
C0551
Monoclonal Anti-CD11b
MSC
HLA class I
Antigen processing/presentation
HLA-A, HLA-B, HLA-C
MSC
CD11b (Integrin alpha M)
Cell adhesion
ITGAM
MSC
CD59
Cell adhesion
CD59
MSC
Mesenchymal (stromal) stem cell
MSC, ERC
CD44
Cell adhesion (hyaluronic acid receptor)
CD44
C7923
Monoclonal Anti-CD44
NPC
beta-III Tubulin
microtubule protein
TUBB3
T2200
Anti-β-Tubulin III
NPC
beta-III Tubulin
microtubule protein
TUBB3
T8660
Monoclonal Anti-β-Tubulin III
NPC
beta-III Tubulin
microtubule protein
TUBB3
T5076
Monoclonal Anti-β-Tubulin Isotype III
NPC
Microtubule Associated Protein 2
microtubule protein
MAP2
M2320
Monoclonal Anti-MAP2 (2a+2b)
NPC
Microtubule Associated Protein 2
microtubule protein
MAP2
M9942
Monoclonal Anti-MAP2
NPC
Musashi 1
RNA binding
MSU1
NSC
vimentin
Intermediate filament protein
VIM
V4630
Anti-Vimentin
NSC
vimentin
Intermediate filament protein
VIM
V6389
Monoclonal Anti-Vimentin
NSC
vimentin
Intermediate filament protein
VIM
V5255
Monoclonal Anti-Vimentin
NSC
Nestin
NSC
Neural stem cell
NSC
A2B5
Cell surface ganglioside marker
A8229
Monoclonal Anti-A2B5
NSC, GSC, MSC
Integrin beta1 (CD29)
Cell surface adhesion factor
I7533
Anti-phospho-Integrin β1 (pSer785)
NSC, ESC, PSC, HSC, CSC
CD133/Prominin 1
OLPC
Oligodendrocyte Marker O4
cell surface protein
O7139
Monoclonal Anti-Oligodendrocyte Marker O4
OLPC
Galactosylceramide
mylelin membrane lipid
GALC
G9152
Anti-Galactocerebroside
PESC
Prostate endothelial stem cell
PESC, ECS, EGC, GC
Oct4
Maintains pluripotency
POU5F1
P0873
Anti-POU5F1 (Oct4)
PSC
Nanog
Maintains pluripotency
NANOG
N3038
Monoclonal Anti-Nanog
PSC
SOX2
PSC, EGC, EC
Tra-1-60
karatan sulfate antigen
PSC, EGC, EC
Tra-1-81
karatan sulfate antigen
RPEC
Retinal pigment epithelium
RPC, OPC
MITF
SC
Stem cell
SMPC SMPC SMPC
General Stem Cell Biology
Category
NES
ITGB1 PROM1
SOX2
Transcription factor
MITF
M6065
Monoclonal Anti-Microphthalmia antibody
Smooth muscle myosin
contractile protein
MYH11
M7786
Monoclonal Anti-Myosin (Smooth)
Calponin, basic
cytoskeletal protein
CNN1
C6047
Monoclonal Anti-Calponin
Actin, smooth muscle alpha
cytoskeletal/contractile protein
ACTA2
A5228
Monoclonal Anti-Actin, α-Smooth Muscle
Key:
Astroglia Progenitor Cell
ESC
Embryonic Stem Cells
OPC
Osteoclast Progenitor Cell
ASC
Adipose Stromal Cell
GC
Germ Cells
PESC
Pluripotent Embryonic Stem Cell
BDPC
Bile Duct Progenitor Cell
HSC
Hematopoietic Stem Cell
PESC
Prostate Endothelial Stem Cells
BSC
Blood Stem Cell
LSC
Leukemic Stem Cell
PSC
Pluripotent Stem Cell
CM
Cardiomyocyte
MPC
Mesoderm Progenitor Cell
RPC
Retinal Progenitor Cell
EB
Embryoid Body
MSC
Mesenchymal Stem Cell
RPEC
Retinal pigment Epithelilial cell
EC
Tetracarcinoma Stem Cells
NPC
Neural Progenitor Cell
SMPC
Smooth Muscle Progenitor Cell
EGC
Embryonic Germ Cells
NSC
Neural Stem Cell
ERC
Endometrial Regenerative Cell
OLPC
Oligodendrocyte Progenitor Cells
AGPC
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Antigen
Oligosaccharide structure
Globoseries glycolipids SSEA3
Galβ1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer
SSEA3, SSEA4
NeuNAcα
Forssman
3Galβ1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer GalNAcα1 → 3GalNAcβ1 → 3Galαl → 4Galβ1 → 4Glcβ1 → Cer
Latoctoseries glycolipids SSEA1
Galβ1 → 4GlcNAcβ1 → 4Galβ1 → 4Glcβ1 → Cer 3 →
General Stem Cell Biology
Glycolipid Antigens of Mouse and Human EC and ES Cells
Fucαl Ganglioseries glycolipids 9-O-acetyl GD3 (ME 311)
(9-O-acetyl) NeuNAcα2 → 8NeuNAcα2 → 3Galβ1 → 4Glcβ1 → Cer
GT3 (A2B5)
NeuNAcα2 → 8NeuNAcα2 → 8NeuNAcα2 → 3Galβ1 → 4Glcβ1 → Cer (adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)
All of these glycosphingolipid antigens are synthesized by extension from a common precursor, lactosylceramide. The likely parts of these structures comprising the epitopes recognized by the different antibodies are highlighted. The enzymes responsible for the addition of the third sugar residues (galactosyl-, glucosaminyl-, and sialyltransferase) appear to regulate the rate-limiting step that controls which synthetic pathway predominates. Human EC and ES cells are characterized by expression of the glycolipid antigens SSEA3 and SSEA4, and not the lactoseries antigen SSEAl (Lex). By contrast, mouse EC and ES cells are typically characterized by their expression of SSEAl. Mouse cells also express the globoseries Forssman antigen, which is not synthesized by human cells. Thus, despite the apparent distinction between mouse and human EC and ES cells in terms of their terminal sugars that comprise the different epitopes, the undifferentiated cells of both species do seem to share in common the expression of globoseries core structures.
Differential Expression of Characteristic Surface Marker Antigens of Mouse and Human EC and ES Cells Antigen
Mouse
Human
Forssman
Yes
No
Globoseries glycolipid
SSEA 1 (Le )
Yes
No
Lactoseries glycolipid
SSEA3
No
Yes
Globoseries glycolipid
SSEA4
No
Yes
Globoseries glycolipid
TRA-1-60
No
Yes
Keratan Sulfate
TRA-1-81
No
Yes
Keratan Sulfate
GCTM2
No
Yes
Keratan Sulfate
Liver alkaline phosphatase (L-ALP)
Yes
Yes
No antibody is available for murine ALP, but human L-ALP is recognized by antibodies TRA-2-54 and TRA-2-49.
MRC Class 1
No
Yes
H-2 in the mouse; HLA in humans
x
Comments
(adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)
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Common Antibodies Used to Detect Antigens Expressed by Human EC and ES Cellsiso SSEA3
Rat IgM
Undifferentiated hES-EC
MC480
SSEA1 (Le )
Mouse IgM
Differentiated hES-EC; including trophoblast
A2B5
GT3
Mouse IgM
Neural
ME311
9-O-acetylGD3
Mouse IgG
Neural
VINI556
GD3
Mouse IgM
Neural
VIN2PB22
GD2
Mouse IgM
Neural
MC8 1 3-70
SSEA4
Mouse IgG
Undifferentiated hES-EC
TRA-1-60
TRA-1-60
Mouse IgM
Undifferentiated hES-EC
TRA-1-81
TRA-1-81
Mouse IgM
Undifferentiated hES-EC
TRA-2-54
L-ALP
Mouse IgG
Undifferentiated hES-EC
TRA-2-49
L-ALP
Mouse IgG
Undifferentiated hES-EC
TRA-1-85
Ok[a]
Mouse IgG
Panhuman
BBM1
β2-microglobulin
Mouse IgG
HLA
x
W6/32 HIA-A, -B, -C Mouse IgG
General Stem Cell Biology
MC631
HLA
(adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006)
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NIH Code
Karyotype
AC133
Alk Phos
CD90
Cripto
Cryopreservation
Feeder-free
H1
WA01
XY
+
+
+
+
+
+
H7
WA07
XX
+
+
+
+
+
+
H9
WA09
XX
+
+
+
+
+
HES-1
ES01
XX
+
+
+
+
HES-2
ES02
XX
+
+
+
+
General Stem Cell Biology
ES Line
GCTM-2
HES-3
ES03
XX
+
+
+
+
HES-4
ES04
XY
+
+
+
+
+
+
+
+
HES-6
ES06
XY
HSF-6
UC06
XX
hESBGN.01
BG01
XY
hESBGN.02
BG02
XY
+
Miz-hES-1
MI01
XY
+
ES Marker
Mouse
Human
Alk Phos
POS
POS
BCRP-1
POS
POS
CX43
POS
POS
CX45
POS
POS
FGF-4
POS
POS
FOXD3
POS
NEG
gp130
POS
POS
LIFR
POS
NEG
Nanog
POS
POS
OCT 3/4
POS
POS
REX-1
POS
POS
SOX 2
POS
POS
SSEA-1
POS
NEG
SSEA-3
NEG
POS
SSEA-4
NEG
POS
STAT3
POS
POS
TERT
POS
POS
TRA-1-60
NEG
POS
TRA-1-81
NEG
POS
UTF-1
POS
POS
+
1981, ES Cell First isolated in mice
1995, First non-human p
200
2
2000, NIH
1998, First hum
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GDF3
Genesis
hTERT
Nanog
OCT4
REX-1
SOX 2
SSEA-3
SSEA-4
+
+
+
+
+
+
+
+
+
+
+
+
TG343
TRA-1-60
TRA-1-81
UTF-1
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+
+/-
+
+
+
+
+
+
+
+
+
+
+
+
+
General Stem Cell Biology
+
+
primate ES line
00, NIH issue guidelines for Federal funding of ESC research
2001, Federal Government announces restrictions on funding for Stem Cell Research 2003, Ohio establishes the Center for Stem Cell and Regenerative Medicine 2003 - 2007, IL, MD, CT, IN, and other states pass legislation for state funding
2007, ES-like cells derived from skin fibroblasts 2004, CA and NJ appropriate funding at the state level to support SC Research 2003, Ohio establishes the Center for Stem Cell and Regenerative Medicine
H issue guidelines for Federal funding of ESC research
man ES line
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Stem Cell Biology General Stem Cell Biology
Where we’ve been and where we’re heading! By: Dr. Greg Wall, PhD, BioChemsultants, LLC. Unraveling and exploring the mysteries of life have been driving forces behind scientific studies since Aristotle first attempted to understand the origins of life. Through the eyes of the microscope, cellular processes have been observed, investigated, understood, and manipulated. This quest has intensified with the discovery of embryonic and adult stem cells. In recent years the genetic modification of stems cells, and the latest discovery, the transformation of somatic cells into stem cells have emerged as primary areas of therapeutic biomedical research. Potentially, stem cells can be transformed into any of the over 200 specialized cells in the body. Their use in cell-based therapies, drug development, and new disease models is still in its infancy. The future potential in basic and applied clinical research is fueled by over 2,000 articles published yearly. Since the 1950s, adult stem cells have been used in bone marrow transplants and to develop a variety of treatments for diseases. Bone marrow transplants are used to treat malignant diseases such as acute and chronic leukemias, Hodgkin's disease and non-Hodgkin's lymphoma, myelodysplasia, multiple myeloma, myelofibrosis, and renal cell carcinoma. Increasingly, success has been seen in the treatment other diseases including aplastic anemia, sickle cell anemia, immune deficiency disorders, and autoimmune diseases such as scleroderma and multiple sclerosis. It was not until 1963 that the first qualitative description of bone marrow cell’s self-renewing activities by Ernest McCulloch and James Till, Ontario Cancer Institute, established the concept of stem cells.1 The first embryonic stem cells derived directly from the blastocyst in culture was reported by Martin Evans and Matthew Kaufman, University of Cambridge, in 1981.2 During the 1980s and 1990s, techniques for targeting and altering genetic material and in growing human cells in the laboratory flourished and laid the groundwork for human stem cell research. It was observed that telomerase activity is expressed in unspecialized stem cells and progenitor cells, but suppressed in somatic cells, and specific cell surface antigens SSEA-3, SSEA-4, Tra-1-60 and Tra-1-81 can be used to identify stem cells. The first cloning of a mammal by somatic cell nuclear transfer (SCNT) in 1997 by Ian Wilmut, Roslin Institute, demonstrated mammalian differentiated cells can be transformed to an undifferentiated state by trans-acting factors present in the oocyte. This was followed by the search for factors that could mediate similar reprogramming of somatic cells.3 The following year, James Thomson, University of Wisconsin – Madison, used trans-acting factors to reprogram isolated cells from the inner cell mass of early embryos, leading to the development of the first human stem cell lines and John Gearhart, Johns Hopkins University, derived human embryonic germ cells from primordial germ cells.4,5 Pluripotent stem cell lines were then prepared from both. Subsequent research has yielded a better understanding of the characteristics, growth, and differentiating properties of adult and embryonic stem cells. Researchers are now beginning to identify the transcription factors necessary to reprogram human somatic cells to induced pluripotent stem cells.
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The reprogramming and induction of human somatic cells to stem cells that exhibit the characteristics of embryonic stem cells have been pursued by many laboratories and was reported in separate papers by Shinya Yamanaka, Kyoto University, and James Thomson, University of Wisconsin – Madison, in 2007. Their findings were based on the similarity to human embryonic stem cells in morphology, proliferation mechanisms, surface antigens, pluripotent cell-specific gene epigenetic status, and telomerase activity. Yamanaka utilized Oct3/4, Sox2, Kif4, and c-Myc transcription factors to reprogram human dermal fibroblast cells. In this method, c-Myc and Klf4 may be involved in modifying chromatin structures so that Oct3/4 and Sox2 bind to their targets. Thomson transduced IMR90 fibroblast and postnatal fibroblast cells with a combination of Oct4, Sox2, Lin28, and NANOG. Lin28 and NANOG have been shown to improve cloning efficiency and recovery. The induced pluripotent human stem cells by both methods maintained their ability to differentiate into all three germ layers in vitro. One potential limitation of the Yamanaka method using the c-Myc factor would be mutation through viral integration. The altered or forced expression of c-Myc may result in unwanted differentiation of the induced pluripotent stem cells. By using different transcription factors to reprogram and generate cells having the same characteristics as stem cells, the viability of this technology has been validated as a method to induce somatic cells. The induction process and how to harness it is now under further investigation. Paralleling this achievement, research groups have continued to make progress in developing methods to improve the efficiency of human therapeutic cloning via SCNT. Advances have been made in the preparation of recipient cytoplasts, preparation and transfer of donor DNA, and parthenogenic activation of embryonic development. Recently, Andrew French, Stemagen Corporation, has been able to clone blastocysts from somatic cells.8 The ability to prepare nuclear transfer stem cells by this method is promising to regenerative medicine and cell-based drug discovery. The preparation of human induced pluripontent stem cells is a milestone in stem cell biotechnology as is its impact on medical applications. This opens up the possibility of using them as well as embryonic stems cells in treating medical conditions such as cancer and birth defects, along with generating tissue for arthritis, burns, diabetes, heart disease, strokes, spinal cord injury, and disabilities like Alzheimer’s and Parkinson’s disease. Currently hematopoietic stem cells are the only stem cells being used in the treatment. Human induced pluripotent stem cells may have a role in drug development for efficacy or toxicity screening; thus, eliminating the need for animal testing and shortening the time to commercialization. They open the window for personalized medical treatment with cell-based therapies based on stem cells that contain the genetic makeup or genetically engineered DNA of the patient.
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References 1. Becker, A.J., et al., Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells. Nature, 197, 452-454 (1963). 2. Evans, M.J., and Kaufman, M.H., Establishment in culture of pluripotential cells from mouse embryos. Nature, 292, 154-156 (1981).
3. Wilmut, I., et al., Viable offspring derived from fetal and adult mammalian cells. Nature, 385, 810-813 (1997). 4. Thompson, J.A., et al., Embryonic Stem Cell Lines Derived from Human Blastocysts. Science, 282, 1145-1147 (1998). 5. Gearhart, J., et al., Derivation of pluripotent stem cells from cultured human primordial germ cells. P.N.A.S., 95, 13726-13731 (1998). 6. Yamanaka, S., et al., Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 131, 861-872 (2007)
General Stem Cell Biology
The study of embryonic stem cells will continue to further our understanding of how they transform. Both private and government funding of embryonic stem cell research will be critical to reach this goal to provide a platform for the future. Human induced stems cells eliminate the need to use human embryos; thus, minimizing or even eliminating cultural, political, religious, and ethical concerns. The commercial and social impact to be gained by stem cell research coming to fruition is immeasurable. The ability to use stem cells instead of scalpels will affect how we practice medicine and approach personalized healthcare. As our understanding of genomics and proteomics has enriched our appreciation of the complexities of life, our exploration of stem cells will solve even more of its mysteries.
7. Thomson, J.A., et al., Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science, 318, 1917-1920 (2007). 8. French, A.J., et al., Development of Human cloned Blastocysts Following Somatic Cell Nuclear Transfer (SCNT) with Adult Fibroblast. Stem Cells published online Jan 17, 2008, http://stemcells. alphapharmedpress.org/cgi/reprint/2007-0252v1.pdf 9. Dr. Greg Wall, Ph.D., BioChemSultants, LLC
Protocols for Stem Cell Culture
Stem and feeder cell culture and cryopreservation 2x Freezing media preparation 20% fetal bovine serum (F2442) 20% DMSO (D2650) 60% ES cell media Store freezing media at -20 degrees C and thaw to 37 degrees C or room temperature before use. Ready-to-Go Freezing media Serum-free cyropreservation medium (C6295) Contains MEM, 8.7% DMSO, and methylcellulose DMSO-free cryopreservation medium (C6039) Contains MEM, glycerol, calf and fetal bovine serum Standard cryopreservation medium (C6164 Contains MEM, DMSO, calf and fetal bovine serum Complete media for mouse embryonic fibroblast (MEF) feeder cells
The information contained below is a compilation of various publications. For your convenience Sigma product numbers have been added where applicable, however these products have not been tested in stem cell applications.
Culture media formulation for ES (Embryonics Stem) and MEF Mouse Embryonic Fibroblast) feeder cells
For 500 ml of ES complete media: 100 ml fetal bovine serum (F2442) 5 ml 100x non-essential amino acids, NEAA (M7145) 5 ml 100 x Pen-Strep (P4333 or P0781) 5 ml 100 x sodium pyruvate (S8636 or P4562) 5 ml beta-mercaptoethanol Stock solution = 3.5 ul diluted to 5 ml neat beta-maerpactoethanol (M7522) with tissue culture grade water (W3500) - make fresh, do not store diluted solution 380 ml DME, high glucose (D5796) [See M3942 for reduced serum MegaCell formulation] 1 ml 106 Units/ml LIF (L5158) For positive and negative selections, add: 5 ml 100x G418 (G8169) 0.1 ml 5000x Ganciclovir ( use G2536 at 10 mM in PBS, P5368) Filter-sterilize as needed, store media at 4˚ C, pre-warm to 37 degrees C before use.
For 500 ml media: 445 ml DMEM (D5796) 50 ml fetal bovine serum (F2442) 5 ml 100x Pen-Strep (P4333 or P0781) Gelatin-treated culture ware All dishes/plates need to be treated with 0.2% gelatin for 5 minutes at room temperature. 0.2% gelatin is made from diluting 2% gelatin solution (G1393) with PBS (P5368) and autoclave to sterilize. The volume depends on culture vehicle, plates, slides, etc. It is necessary to cover the entire culture surface.
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General Stem Cell Biology
Stem and feeder cell culture and cryopreservation Expansion and irradiation of MEF feeder cells n Plate one vial primary MEF cells in one T75 flask with 20 ml MEF media. n Expand 1x T75 flask of cells into 1x T175 flasks. n Split 1x T175 flask of cells into 3 T175 flasks. n Split 3x T175 to 6xT175 flasks if more feeders are needed. n Trypsinize and pool all cells, spin down and resuspend cells in 50 ml MEF media. n Irradiate cells (gamma irradiation at 3000 rads). n Spin down and resuspend cells in MEF media. n Add an equal volume of 2x Freeze media. n Mix well and freeze for overnight in 0.5 ml aliquots at -80 degrees C. n Store in liquid nitrogen.
Freezing ES cells on 96-well plates Aspirate media and wash once with PBS. Freeze down the 3 x gelatinized only plate at -20 degrees C for DNA prep. For the 2 x plates with feeder cells, add 25 ul trypsin STV and incubate at 37 degrees C for 5 minutes. Disaggregate cells with 100 ul ES media and spin cells at 1K for 5 minutes. Take off supernatant. Be careful not to suck cells from bottom. Resuspend cells in 75 ul ES media.
Each T175 flasks of cells can be aliquoted into 6 vials. Each vial can then be plated onto 1x T25 flask. You may need to adjust these numbers according to plating efficiency. Routine maintenance of ES cells To maintain a healthy and undifferentiated culture, cells need to grow at high density (above 106 to 107/ 8cm2 or one well of a 6-well plate) and they should be passaged frequently. As a general rule, the media should be changed daily and cells should be passed every other day with a 5 fold split. Cells are oftens ‘shocked’ when coming out of freeze and may show a lag time from initial culture set up to the first passage, perhaps 3 to 4 days. Tips: Do a visual check of ES cultures. Observe for nesting (cells are in contact with each other). Undifferentiated ES cells should appear as compact colonies with prominent nucleoli. When cells are colonial or ‘nested’ individual cell borders should not be distinguishable but the colony should have a well defined boundary and may appear a little refractile under a phase microscope. To separate ES cells from MEF’s plate co-cultures on non-coated plates for 30 minutes at 37 degrees C. Feeder cells will attached leaving a relatively pure ES cell population in suspension.
Add 75 ul 2x freezing media. Cover plates with parafilm, place plates in plastic bags and then in Styrofoam box and put in -80 degrees C freezer. Thawing clones n Prepare feeder cells (50 ul /well) on 96-well plates. n Remove plates from -80 degrees C and let plates sit in warm bath. n After clones are thawed spin plates at 1K for 5 minutes. n Remove supernatant from well. n Resuspend clone in 100 ul ES media and add clone to fresh well with feeder cells. n After cells grow to confluence pass cells to 1 well of 24-well plate.
References 1. Stanford Transgenic Reesearch Center – online protocols 2. Nagy Lab, Samuel Lunenfeld, Mount Sinai Hospital – online protocols 3. Darwin Transgenic Mouse Core Facility, Baylor College of Medicine – online protocols 4. Comparative and Functional Genomics Dept., Max Planck Institute – online protocols
To pass ES cells: n Aspirate media off the plate and rinse once with PBS. n Trypsinize cells with 0.05% trypsin (T3924) at 37 degrees C for 3 to 5 minutes. n Add 5 ml of ES media and pipet vigorously to get single cell suspension. n Spin at 1000 rpm for 5 minutes. n Plate cells out onto irradiated feeder cells.
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Mouse ES cell freeze/thaw protocols
Stem and feeder cell culture and cryopreservation Many of the established hES cells have low recovery upon thawing, as low as 0.1 to 1%. This may be because of the method of passaging the cells. Mechanical picking or using collagenase dispersion usually results in large cell aggregates, which presumably do not get cryopreserved as efficiently as smaller clumps. Trypsinized cells in our lab have a recovery rate of about 10 to 20% or higher and do not require more complicated procedures such as vitrification (Figure 38-9). Freezing Medium The best recovery rate was observed in freezing medium consisting of 90% FBS-10% dimethyl sulfoxide (DMSO). However, Oct-4 expression in the thawed cells was lower than in cells frozen in hES cell growth medium with 10% DMSO. Nevertheless, by the next passage, the expression and distribution of Oct-4 and other markers of undifferentiated cells were indistinguishable between these two freezing conditions. We routinely use the 90% FBS-10% DMSO medium. Freezing Protocol Select a high-quality confluent culture with good morphology for freezing. We also recommend taking a picture of a sample field and staining for molecular markers characteristic of undifferentiated hES cells for future reference. Materials needed: n Chilled freezing medium: 90% FBS, 10% DMSO n Cryovials, labeled with the line, passage number, and date n Cryovial rack (rack with ice reservoir by Corning)(Sigma Cat. No. CLS430525) n Styrofoam rack from packaging for 15 ml centrifuge tubes n -80 °C freezer 1. Trypsinize the cells; centrifuge in PMEF medium (see earlier explanation). 2. Resuspend the pellet in the cold freezing medium. We recommend freezing one confluent 35 mm plate per vial in 0.5 ml of freezing medium. Work quickly and keep the cells on ice after the addition of the freezing medium. 3. Aliquot cell suspension into prechilled freezing vials and sandwich the vials between two Styrofoam racks: tape to prevent the two racks from separating and transfer to a –80 °C freezer overnight. Transfer the cryovials to liquid nitrogen for long-term storage.
Thawing hES Cells
Thawing hES cells is a relatively simple procedure. The main rule to follow is to do everything quickly.
30% hES cell- or PMEF-conditioned medium. 3. Change the medium on the PMEF plate to the thawing medium; equilibrate in the CO2 incubator for one hour. For 35 mm plates, use 1.5 ml medium; for four-well plates, use 0.5 ml medium per well. 4. Prepare a 50 ml conical tube with 10 to 15 ml of warm hES cell growth medium. Thawing 1. Thaw the vial in a 37 °C water bath, constantly agitating while ensuring that the neck of the vial is above the water level. Check the content of the vial after about 40 seconds and at 10-second intervals until only a small piece of ice remains. 2. Quickly spray the vial with 70% isopropanol, then using a 1 ml pipetteman*, add warm hES cell medium to the contents of the vial dropwise with gentle agitation. Do it quickly but very gently. Immediately transfer the contents into the prepared 50 ml tube with warm hES cell medium; centrifuge at 160 x g for 5 minutes. 3. Remove the medium completely without touching the pellet. 4. Add 0.5 ml of hES cell thawing medium, gently resuspend the cells using a 1 ml pipetteman (2 to 4 repetitions), and transfer to prepared PMEF plates with equilibrated hES cellthawing medium. Spread the cells evenly throughout the well by moving the plate several times in two directions at 90 degrees to each other; avoid swirling. 5. Check the cells the next day; if there are many dead cells or the medium has changed color, change two-thirds of the medium; otherwise, do not change it for another day. 6. The colonies usually begin to appear in 3 to 4 days and can be ready for splitting in 5 to 10 days (Figure 38-7).
General Stem Cell Biology
Freezing hES Cells
hES Cell Quality Control Although the morphology of hES cells is often used for evaluating the quality of the culture and its readiness for passaging or freezing, this criterion alone cannot be used for an assessment of ES cell pluripotency. Staining for the expression of Oct-4 or alkaline phosphatase even in colonies of "perfect" morphology can result in one or both of these markers appearing in the cells only at the periphery of the colony. It is important, therefore, to regularly assess the cells by analyzing the expression of markers of pluripotent cells. We look at Oct-4, SSEA-3, SSEA-4, TRA 1-60, and TRA 1-81 by immunostaining, or we perform an enzyme assay for alkaline phosphatase. The procedures for such assays and available antibodies are described elsewhere. *adapted from Essentials of Stem Cell Biology, R. Lanza, ed. Academic Press, 2006
Preparation 1. Prepare mitomycin C-treated PMEFs a day before thawing. 2. Make a thawing medium. We use 70% hES cell growth medium supplemented with 2x hLIF and 8 ng/ml bFGF with
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Sigma-Aldrich Stem Cell Biology Platform!
Infinite Expansion, Infinite Potential! Sigma-Aldrich is pleased to announce the latest and most comprehensive selection of products for stem cell research! This platform is comprised of products necessary to the stem cell workflow from isolation and cryopreservation to in-vivo tracking with all of the related profiling tools in between! Please visit sigma-aldrich.com/stembio to view our complete portfolio! In addition to our product profiles you will also find the latest references, application notes, and a new Bulletin Board feature that will enable stem cell scientists like you to consult and exchange ideas and information! Our Innovation, Your Research — Shaping the Future of Life Science
sigma-aldrich.com
Our NEW Stem Cell Biology Platform begins with our comprehensive web site! Easily find products and detailed usage information in the following stem cell areas: N
Isolation
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Expansion
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Characterization
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Differentiation
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Functional Profiling
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In-vivo tracking and
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Much More!
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