Science in Society 26 April 2007
To ID or not to ID: that is the question Dr. Cora A. De Ungria DNA Analysis Laboratory UP Diliman QC
http://www.dnaforensic.org
Research ‘studious examination esp. experimentation aimed at discovery and interpretation of new facts, or the practical application of new theories…’ (SCIENTIFIC METHOD)
Research in Forensic Biotechnology
?
CSI PINAS?
Definitions • Biotechnology - any technology
that uses an organism or its parts to develop a new product or a new trait in an organism or to develop a new technique • Forensic - is the application of a broad spectrum of science to answer questions of interest to the legal system.
FORENSIC APPLICATIONS: • Investigations Civil/ criminal • Disputed parentage cases • Mass disasters
IDENTIFICATION!
Rape- homicide of 9 year old – July 10, 1999 People vs Vallejo, 9 May 2002 First case involving DNA evidence which reached the Supreme Court
Biology of a Human cell • Nucleus contains 23 pairs of chromosome •male 1-22 + XY; female 1-22 + XX • Mitochondrion/ mitochondria (pl): maternal
What is DNA? • Deoxyribonucleic acid • genetic blueprint of all living organisms • nitrogen bases G,A,T,C
Short Tandem Repeats (STR) ONE STR MARKER: TAGA
TAGA
TAGA
TAGA
TAGA
TAGA
TAGA
1 2
• size of marker = small • not code for proteins = not visible • highly variable in population
4/3
FBI Combined DNA Identification System
Properties of DNA •1 Chemical stability •2 Identity •3 Uniqueness
1. DNA in Courts
EC PROJECT: Human Rights and Democracy in Asia- Research, Training and Extension Service in Forensic DNA Analysis in the Philippines
1.Research to increase the standards of testing 2. Organize training courses for analysts, lawyers and judges 3. Conduct DNA tests for capital cases after SC review
STRATEGY: Yes to interview Search for biol. evidence: court, police, hospital, lawyers
Investigate ways to access evidence in the absence of judicial framework for the conduct of Post-Affirmation DNA tests
HOW DO YOU TEST FOR PATERNITY?
ALLEGED FATHER (AF)
MOTHER
CHILD
Case 1: Possibility of Paternity Alleged Father
Mother
Real father Child
or Chance match 66 77
88 99
66 99
Case 2: Exclusion of Paternity Alleged Father
10 7
10 7
Mother
Child
8 9
6 9
8 9
6 9
Absolutely not the real father
2. DNA in Investigations
DNA in Investigations • ‘Incorporating DNA Evidence in Routine
Examination of Child Sexual Assault cases’ • Worked with MDs of CPU units in Manila, Baguio, Cebu and Davao
39 cases
YES
male DNA
20
19
-
sperm detection
6
21
12
internal ejaculation
18
5
16
post-coital washing
28
8
3
NO
Unknown
3. Philippine Jurisprudence in Filiation Cases • Tijing vs Diamante (2001) • Herrera vs Alba (2005) • Agustin v Prollamante (2005) – (The growing sophistication of DNA technology is a “much needed equalizer” for “such ostracized and abandoned progeny” (i.e., illegitimate children)
Acknowledgments: •UP: NSRI, CPU, ASP, IHG •ADB, WB •European Commission •PCASTRD, DOST •NGOs (FLAG, PJPS) •PRC, NCIP regional