Enzyme Lab

Biology 111- Enzymes Lab Experiment 1: Tube 1

Positive

5 ml catechol

Tube 2

Negative

5 ml dH₂O

Tube 3

Negative

5 ml catechol

5 ml potato extract 5 ml potato extract 5 ml dH₂O

Tube 4

Negative

5 ml hydroquinone

5 ml potato extract

Light orange Foggy clear Clear brownish Foggy clear

Experiment 2: Tube 1

Positive

5 ml catechol

Tube 2 Tube 3 Tube 4

Positive Positive Negative

5 ml catechol 5 ml catechol 5 ml catechol

PE (room temp) PE (40˚ C) PE (60˚C) PE (80˚C)

Orange Orange Orange Foamy, foggy, clear

At what temperature does catecholase “denature”? 80˚C

Experiment 3: Tube 1 (positive) Tube 2 Tube 3 Tube 4

hydrogen peroxide 5ml hydrogen peroxide 5ml hydrogen peroxide 5ml hydrogen peroxide 5ml

PE (room temp)

Foam

PE (prev. boiled)

Foggy clear white No reaction

MnO₂ (room temp) 5ml MnO₂ (prev. boiled) 5ml

clear

What is the white “foam” coming out of the tubes? Oxygen bubbles

Experiment 4: Predications: 1. 2. 3. 4.

Tube Tube Tube Tube

1 1 2 4

will: will: will: will:

turn orange be a foggy color be white and foggy be bubbly clear

Questions for lab: Pg. 114 a. The enzymes are proteins in either tertiary or quaternary structure.

The substrate is the reactant molecule that interacts directly with the enzyme in the region of the active site in order for the product molecule to form. b. This way the tubes will not cool down due to room temperature. After 5 minutes the tubes will be accurately read. c. Tube 1: orange Tube 2: orange Tube 3: orange Tube 4: foamy, foggy, clear e. Tubes 1 through 3 were all measured at different temperatures, yet they all had the same results. f. From 60˚ to 80˚, measurements can go higher to 100˚ h.

80˚ because the solution turned foamy and foggy

i.

Bubbling was the result of denaturing the enzyme

j.

An advantage to this would be more accurate answers

Future Study: 2. Biological catalysts which are either tertiary or quaternary structure. The reactant which binds with enzyme (this forms product).