Ecg Product Info

(−)-Epicatechin gallate from green tea Product Number E3893 Storage Temperature 2-8 °C Product Description Molecular Formula: C22H18 O10 Molecular Weight: 442.4 CAS Number: 1257-08-5 Synonym: (2R,3R)-2-(3,4-dihydroxyphenyl)3,4-dihydro-1(2H)-benzopyran-3,5,7-triol 3-(3, 4,5-trihydroxybenzoate) (-)-Epicatechin gallate is a polyphenol compound, or catechin, that occurs naturally in tea (Camellia sinensis). This compound and other tea polyphenols 1,2 have been studied for their antioxidant properties. Epicatechin gallate has been shown to inhibit tyrosine phosphorylation of platelet-derived growth factor Rβ in rat and human vascular smooth muscle cells and of vascular endothelial growth factor receptor 2 (VEGF 3,4 R2). A study of the action of epicatechin gallate and other compounds on plasma coagulation by 5 Staphylococcus aureus has been described. A protocol for the purification of epicatechin gallate that uses liquid-liquid partitioning, high-speed countercurrent chromatography, and gel 6 chromatography has been published. The analysis of epicatechin gallate and other green tea constituents by microemulsion electrokinetic chromatography has 7 been described. An HPLC/electrochemical detection technique for the analysis of epicatechin gallate and 8 other tea catechins has been reported. An HPLC/MS study has analyzed the presence of epicatechin 9 gallate and other polyphenols in grapes. Precautions and Disclaimer For Laboratory Use Only. Not for drug, household or other uses. Preparation Instructions This product is soluble in water (5 mg/ml), with heat and sonication as needed, yielding a clear, colorless solution.

References 1. Nakagawa, T., and Yokozawa, T., Direct scavenging of nitric oxide and superoxide by green tea. Food Chem. Toxicol., 40(12), 17451750 (2002). 2. Anderson, R. F., et al., Green tea catechins partially protect DNA from • OH radical-induced strand breaks and base damage through fast chemical repair of DNA radicals. Carcinogenesis, 22(8), 1189-1193 (2001). 3. Sachinidis, A., et al., Inhibition of the PDGF β-receptor tyrosine phosphorylation and its downstream intracellular signal transduction pathway in rat and human vascular smooth muscle cells by different catechins. FASEB J., 16(8), 893-895 (2002). 4. Lamy, S., et al., Green tea catechins inhibit vascular endothelial growth factor receptor phosphorylation. Cancer Res., 62(2), 381-385 (2002). 5. Akiyama, H., et al., Antibacterial action of several tannins against Staphylococcus aureus. J. Antimicrob. Chemother., 48(4), 487-491 (2001). 6. Baumann, D., et al., A simple isolation method for the major catechins in green tea using high-speed countercurrent chromatography. J. Nat. Prod., 64(3), 353-355 (2001). 7. Pomponio, R., et al., Microemulsion electrokinetic chromatography for the analysis of green tea catechins: Effect of the cosurfactant on the separation selectivity. Electrophoresis, 24(10), 1658-1667 (2003). 8. Sano, M., et al., Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection. Analyst, 126(6), 816-820 (2001). 9. Wu, Q., et al., Determination of proanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energy. Anal. Chem., 75(10), 2440-2444 (2003). GCY / RXR 5/06

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