Chem 211 -internal 2004

Universal Science College (B. Sc. Biochemistry) Third Semester 2004

Internal Examination Subject: Chem 211 (Analytical Biochemistry) Date: Feb 16, 2005

Full Marks: 100 Pass Marks: 45 Time: 3 hours

Candidates are required to give their answers in their own words as far as practicable. The figure in the margin indicates full marks. Group A Long Questions (Any three ) (3× ×14 = 42) 1. What are the different labeling techniques? Give examples of each. Illustrate the detail process of enzyme immunoassay. Also explain the enhance immunoassay. 2 a. Write the principle involved in infrared (IR) spectroscopy. b. Mention the principle and instrumentation of atomic absorption spectroscopy. 3. a. Give an account on methodology for the measurement and detection of radioactivity by the method of excitation. b. Describe briefly ionization technique of mass spectroscopy. 4. How are electrochemical techniques classified? What are the differences between potentiometric and voltametric technique? Describe the pH – electrode. Group - B Short Questions: (Any six) (6× ×7 = 42) 1. What are the different models for study of biochemical investigation? Explain all. 2. How the monoclonal antibody are produced? Discuss the strategies for reducing immunogenicity of rodent origin. 3. Show your familiarity with chemical shift. What are shielding and de-shielding effects? 4. An aliquot of a solution containing a light absorbing substance of a concentration of 10 g dm-3 was placed in a 5 cm light path cuvette. The cuvette was placed in a spectrophotometer and a beam of light of wavelength was passed through the cuvette containing the solution. A transmittance value of 90% was recorded. Find: (i) The absorbance of the solution. (ii) The molar extinction coefficient if the molecular mass of the substance is known to be 500. 5 How does mass spectra arise? Give brief account of analysers. 6 What is the function of scintillation cocktail in scintillation counting method? Why cocktail is used? 7. What are Bio-sensors? Describe the principle of bio-sensors. 8. Show you familiarity with fixed angle and vertical rotor. Group – C Very short questions: (Any eight) 1. Criteria of buffers suitable for use in biological research. 2. Electrofusion. 3. Blotting technique. 4. What is ESR spectroscopy? 5 What do you understand by Bathochromic shift? 6. Why should the solution be de-aerated before spectroflourimetric analysis? 7. What information can you obtain from mass spectra? 8. What is radioactivity? What are its units for the measurement of energy? 9. How does nuclear radiation interact with matter and living system? 10. What is amperometric technique? 11. Show your familiarity with swinging bucket rotor. 12. How does light absorption system monitor centrifugation in an analytical centrifuge? ***

(2× ×8 = 16)