Cf Dyes
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CF Dyes TM
Glowing Products for ScienceTM
The next-generation dyes for protein labeling
March 05, 2008
ADVANTAGES
Brightness
CF dyes match or surpass the brightness of Alexa Fluor dyes and Cy dyes.
Photostability
CF dyes are among the most photostable dyes.
Specificity and in-vivo Stability
CF-labeled antibodies offer superior specificity and improved half-life for in-vivo imaging.
Figure 1. HeLa cells were stained with CF 555 phalloidin (red), mouse anti-α-tubulin followed by goat anti-mouse CF 488 IgG (green) and DAPI (blue), respectively.
pH Insensitivity
CF dyes remain highly fluorescent over a wide pH range.
Solubility
CF dyes are highly soluble in both water and a wide range of organic solvents.
Compatibility
PRODUCTS
CF dyes are compatible with common excitation sources and existing filters.
Reactive Dyes
Color Selection
Secondary Antibodies
CF dye series offers the largest dye collection with wavelengths ranging from UV to near IR.
Streptavidin Other Labeled Reagents
Fluorescence Emission Spectra of CF Dyes 120
F lu o re s c e n c e
100 80 60 40 20 0 350
400
450
500
550
600 Wavelength (nm)
650
700
750
800
850
page 2
CF
TM
dyes are next-generation reactive fluorescent dyes developed by scientists at Biotium. These new dyes offer major improvements over the Cy dyes and Alexa Fluor dyes in fluorescent antibody labeling by having a combination of advantages in brightness, photostability, specificity and novel features for in vivo imaging. One feature of CF dyes is that they are not as heavily charged as Alexa Fluor dyes are. As a result, CF dyes do not significantly change the isoelectric points of the antibodies they label, thereby preserving the specificity of the antibodies. Thus, CF dye-labeled antibodies may be particularly advantageous in labeling tissue samples by providing maximal signal to noise ratio. Another advantage is that CF dyes are engineered to be less immunogenic than most other known fluorescent dyes. This characteristics makes CF dye-labeled protein conjugates ideal for in vivo imaging by having a longer half-life in the blood circulation system. CF dyes are also chemically very stable, being able to tolerate both acidic and alkaline conditions. Furthermore, most of the CF dyes are significantly more fluorescent and more photostable than Alexa Fluor
dyes and Cy dyes, making them the best choice for studies that involve long-term imaging. Our CF dye series comprises dyes with wavelengths spanning the UV, visible and near IR spectrum. CF products include both reactive dyes and various CF dye-labeled bioproducts. Table 1 lists some of the initially available products. More CF products will become available during 2008. Please check our website for updates. *CF dye technology is covered by pending US and international patents; Alexa is a registered trademark of Invitrogen Co.; Cy is a trademark of GE Healthcare.
0 minutes
4.5 minutes
AlexaFluor 488
Table 1. List of CF products* Cat. #
Product Name
Abs/Em (nm)
Unit Size
Price($)
92130
CF 555, succinimidyl ester
555/565
1 umole
210.00
92135
CF 647, succinimidyl ester
650/665
1 umole
210.00
20030
CF 555 goat anti-mouse IgG(H+L) 2 mg/mL
555/565
0.5 mL
130.00
20032
CF 555 F(ab')2 fragment of goat antimouse IgG(H+L), 2 mg/mL
555/565
0.25 mL
105.00
20040
CF 647 goat anti-mouse IgG(H+L) 2 mg/mL
650/665
0.5 mL
130.00
20042
CF 647 F(ab')2 fragment of goat antimouse IgG(H+L), 2 mg/mL
650/665
0.25 mL
105.00
CF 488
555/565
0.5 mL
130.00
20043
CF 647 goat anti-rabbit IgG(H+L) 2 mg/mL
650/665
0.5 mL
130.00
00040
CF 555 phalloidin
555/565
300 U
290.00
650/665
300 U
3500 Relative Fluorescence
CF 555 goat anti-rabbit IgG(H+L) 2 mg/mL
CF 647 phalloidin
Comparison of CF 555, Alexa 555 and Cy3 on Goat Anti-mouse IgG
4000
20033
00041
Figure 3. HeLa cells were cultured on glass coverslips, fixed and stained with mouse anti-α-tubulin followed by goat anti-mouse conjugated Alexa Fluor 488 or CF488 antibodies. Images shown are representative HeLa cells taken with the same exposure at 0 and 4.5 minutes of photobleaching on an Olympus 100-watt mercury arc lamp microscope and Image Pro Express software.
290.00
3000
CF 555
2500 2000 1500
Alexa Fluor 555
1000 500
Cy3
0 0
*For a complete list of CF products, please visit Biotium website at www.biotium.com.
2
4
6
8
10
Degree of Labeling (dye/protein)
Figure 4. Relative fluorescence of goat anti-mouse IgG labeled with CF 555, Alexa Fluor 555 and Cy 3, respectively, as a function of degree of labeling.
0 seconds
10 seconds
AlexaFluor 660 Comparison of CF 647, Alexa 647 and Cy 5 on Goat Anti-rabbit IgG 4500
Alexa Fluor 647
CF 660
Relative Fluorescence
4000
CF 647
3500 3000 2500 2000
Cy 5
1500 1000 500 0 0
Figure 2. HeLa cells were cultured on glass coverslips, fixed and stained with mouse anti-αtubulin followed by goat anti-mouse IgG labeled with Alexa Fluor 660 or CF 660. Images shown are representative HeLa cells taken with the same exposure at 0 and 10 seconds of photobleaching using an Olympus microscope and Image Pro Express software.
2
4
6
8
10
Degree of Labeling (dye/protein)
Figure 5. Relative fluorescence of goat anti-rabbit IgG labeled with Alexa Fluor 647, Cy 5 and CF 647, respectively, as a function of degree of labeling.
CF Dyes TM
Glowing Products for ScienceTM
The next-generation dyes for protein labeling
March 05, 2008
ADVANTAGES
Brightness
CF dyes match or surpass the brightness of Alexa Fluor dyes and Cy dyes.
Photostability
CF dyes are among the most photostable dyes.
Specificity and in-vivo Stability
CF-labeled antibodies offer superior specificity and improved half-life for in-vivo imaging.
Figure 1. HeLa cells were stained with CF 555 phalloidin (red), mouse anti-α-tubulin followed by goat anti-mouse CF 488 IgG (green) and DAPI (blue), respectively.
pH Insensitivity
CF dyes remain highly fluorescent over a wide pH range.
Solubility
CF dyes are highly soluble in both water and a wide range of organic solvents.
Compatibility
PRODUCTS
CF dyes are compatible with common excitation sources and existing filters.
Reactive Dyes
Color Selection
Secondary Antibodies
CF dye series offers the largest dye collection with wavelengths ranging from UV to near IR.
Streptavidin Other Labeled Reagents
Fluorescence Emission Spectra of CF Dyes 120
F lu o re s c e n c e
100 80 60 40 20 0 350
400
450
500
550
600 Wavelength (nm)
650
700
750
800
850
page 2
CF
TM
dyes are next-generation reactive fluorescent dyes developed by scientists at Biotium. These new dyes offer major improvements over the Cy dyes and Alexa Fluor dyes in fluorescent antibody labeling by having a combination of advantages in brightness, photostability, specificity and novel features for in vivo imaging. One feature of CF dyes is that they are not as heavily charged as Alexa Fluor dyes are. As a result, CF dyes do not significantly change the isoelectric points of the antibodies they label, thereby preserving the specificity of the antibodies. Thus, CF dye-labeled antibodies may be particularly advantageous in labeling tissue samples by providing maximal signal to noise ratio. Another advantage is that CF dyes are engineered to be less immunogenic than most other known fluorescent dyes. This characteristics makes CF dye-labeled protein conjugates ideal for in vivo imaging by having a longer half-life in the blood circulation system. CF dyes are also chemically very stable, being able to tolerate both acidic and alkaline conditions. Furthermore, most of the CF dyes are significantly more fluorescent and more photostable than Alexa Fluor
dyes and Cy dyes, making them the best choice for studies that involve long-term imaging. Our CF dye series comprises dyes with wavelengths spanning the UV, visible and near IR spectrum. CF products include both reactive dyes and various CF dye-labeled bioproducts. Table 1 lists some of the initially available products. More CF products will become available during 2008. Please check our website for updates. *CF dye technology is covered by pending US and international patents; Alexa is a registered trademark of Invitrogen Co.; Cy is a trademark of GE Healthcare.
0 minutes
4.5 minutes
AlexaFluor 488
Table 1. List of CF products* Cat. #
Product Name
Abs/Em (nm)
Unit Size
Price($)
92130
CF 555, succinimidyl ester
555/565
1 umole
210.00
92135
CF 647, succinimidyl ester
650/665
1 umole
210.00
20030
CF 555 goat anti-mouse IgG(H+L) 2 mg/mL
555/565
0.5 mL
130.00
20032
CF 555 F(ab')2 fragment of goat antimouse IgG(H+L), 2 mg/mL
555/565
0.25 mL
105.00
20040
CF 647 goat anti-mouse IgG(H+L) 2 mg/mL
650/665
0.5 mL
130.00
20042
CF 647 F(ab')2 fragment of goat antimouse IgG(H+L), 2 mg/mL
650/665
0.25 mL
105.00
CF 488
555/565
0.5 mL
130.00
20043
CF 647 goat anti-rabbit IgG(H+L) 2 mg/mL
650/665
0.5 mL
130.00
00040
CF 555 phalloidin
555/565
300 U
290.00
650/665
300 U
3500 Relative Fluorescence
CF 555 goat anti-rabbit IgG(H+L) 2 mg/mL
CF 647 phalloidin
Comparison of CF 555, Alexa 555 and Cy3 on Goat Anti-mouse IgG
4000
20033
00041
Figure 3. HeLa cells were cultured on glass coverslips, fixed and stained with mouse anti-α-tubulin followed by goat anti-mouse conjugated Alexa Fluor 488 or CF488 antibodies. Images shown are representative HeLa cells taken with the same exposure at 0 and 4.5 minutes of photobleaching on an Olympus 100-watt mercury arc lamp microscope and Image Pro Express software.
290.00
3000
CF 555
2500 2000 1500
Alexa Fluor 555
1000 500
Cy3
0 0
*For a complete list of CF products, please visit Biotium website at www.biotium.com.
2
4
6
8
10
Degree of Labeling (dye/protein)
Figure 4. Relative fluorescence of goat anti-mouse IgG labeled with CF 555, Alexa Fluor 555 and Cy 3, respectively, as a function of degree of labeling.
0 seconds
10 seconds
AlexaFluor 660 Comparison of CF 647, Alexa 647 and Cy 5 on Goat Anti-rabbit IgG 4500
Alexa Fluor 647
CF 660
Relative Fluorescence
4000
CF 647
3500 3000 2500 2000
Cy 5
1500 1000 500 0 0
Figure 2. HeLa cells were cultured on glass coverslips, fixed and stained with mouse anti-αtubulin followed by goat anti-mouse IgG labeled with Alexa Fluor 660 or CF 660. Images shown are representative HeLa cells taken with the same exposure at 0 and 10 seconds of photobleaching using an Olympus microscope and Image Pro Express software.
2
4
6
8
10
Degree of Labeling (dye/protein)
Figure 5. Relative fluorescence of goat anti-rabbit IgG labeled with Alexa Fluor 647, Cy 5 and CF 647, respectively, as a function of degree of labeling.
