Api20strep

20600

008041-3 05/01

For in vitro diagnostic use Identification system for streptococci API 20 STREP is a standardized method combining 20 biochemical tests that offer widespread capabilities. It enables group or species identification of most streptococci encountered in medical bacteriology. The complete list of those organisms that it is possible to identify with this system is given in the Identification Table at the end of this package insert.

PRINCIPLE The API 20 STREP strip consists of 20 microtubes containing dehydrated substrates for the demonstration of enzymatic activity or the fermentation of sugars. The enzymatic tests are inoculated with a dense suspension of organisms, made from a pure culture, which is used to rehydrate the enzymatic substrates. The metabolic end products produced during the incubation period are either revealed through spontaneous colored reactions or by the addition of reagents. The fermentation tests are inoculated with an enriched medium which reconstitutes the sugar substrates. Fermentation of carbohydrates is detected by a shift in the pH indicator. The reactions are read according to the Interpretation of Reactions (Table 2) and the identification is obtained by referring to the Analytical Profile Index or using the identification software.

COMPOSITION OF MEDIA AND REAGENTS Suspension Medium 2 ml GP Medium 2 ml

NIN reagent 5 ml

REAGENTS Kit contents (25 tests) : - 25 API 20 STREP strips - 25 incubation boxes - 25 ampules of GP Medium - 25 result sheets - 25 swabs - 1 package insert Additional products (not included in the kit) : - Suspension Medium, 2 ml (Prod. No. 70700) - Reagents : Ninhydrin (NIN) (Prod. No. 70490) Potassium hydroxide (Prod. No. V7053) VP2 or (Prod. No. 70430) _-naphthol (Prod. No. V7054) ZYME A (Prod. No. 70470) ZYME B (Prod. No. 70480) - Mineral oil (Prod. No. 70100) - Sterile Pasteur pipettes - McFarland Standard #4 (Prod. No. 70900) - API 20 STREP Analytical Profile Index (Prod. No. 20690) or identification software (consult bioMérieux) - Ampule rack (Prod. No. 70200) - Columbia blood agar plates - Schaedler broth (optional) Required laboratory equipment : - 35-37°C incubator - Refrigerator (2-8°C) - Bunsen burner - Marker pen - Anaerobic jar - Inoculating loop

VogesProskauer reagent: Potassium hydroxide 30 ml

Voges Proskauer reagent: VP2

Demineralized water

Cystine Tryptone Sodium chloride Sodium sulfite Phenol red Demineralized water pH : 7.8 Ninhydrin 2-methoxyethanol

0.5 g 20 g 5g 0.5 g 0.17 g to make 1000 ml 7g 100 ml

TOXIC R60 : May impair fertility. R61 : May cause harm to the unborn child. R10 : Flammable. R20/21/22 : Harmful by inhalation, in contact with skin and if swallowed. S53 : Avoid exposure (avoid contact with skin and eyes, vapor inhalation and brutal superheating). S45 : In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). 40% aqueous KOH solution 89% in demineralized water 11% CORROSIVE R35 : Causes severe burns. S24/25: Avoid contact with skin and eyes. S26 : In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37/39 : Wear suitable protective clothing, gloves and eye/face protection. S45: Incase of accident or if you feel unwell, seek medical advice immediately (show the label where possible). alpha-naphthol Ethyl alcohol

6g 100ml

5 ml or alpha-naphthol 30 ml

ZYME A reagent 8 ml

ZYME B reagent 8 ml

alpha-naphthol to be diluted with 95% ethyl alcohol

2.05 g 29ml

FLAMMABLE AFTER RECONSTITUTION R21/22: Harmful in contact with skin and if swallowed. S24/25: Avoid contact with skin and eyes. Tris-hydroxymethyl-aminomethane 25 g Hydrochloric acid (37 %) 11 ml Sodium lauryl sulfate 10 g H2 O 100 ml Fast Blue BB 0.35 g 2-methoxyethanol 100 ml TOXIC R60 : May impair fertility. R61 : May cause harm to the unborn child. R10 : Flammable. R20/21/22 : Harmful by inhalation, in contact with skin and if swallowed. S53 : Avoid exposure - obtain special instructions before use. S45 : In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).

STORAGE OF THE STRIPS AND MEDIA The strips and media should be stored at 2-8°C until the expiration date indicated on the packaging.

STORAGE OF THE REAGENTS The reagents should be stored in the dark at 2-8°C (except Potassium hydroxide and ZYME A which can be stored at 230°C) until the expiration date indicated on the packaging. VP2 may be kept up to 1 month after the ampules have been opened and the reagent transferred to the dropper-bottle. After reconstitution, alpha naphthol should be stored at 2-8°C and may be kept for up to 90 days, (or until the expiration date if this comes first) : record the reconstitution date on the bottle label. The ZYME A, ZYME B and NIN reagents may be kept for up to 1 month after the ampules have been opened and the reagents transferred into the dropper-bottles, (or until the expiration date if this comes first) : record the date opened on the bottle label. The NIN and ZYME B reagents are very sensitive to light : wrap the bottles in aluminum foil and only leave them out of the refrigerator while being used. Do not leave them on the bench for prolonged periods of time. The NIN reagent is very sensitive to traces of water and air : transfer the reagent into the dropper-bottle using a dry pipette and keep the bottle tightly closed. The ZYME B reagent is normally yellow in color. Dispose of the reagent if any tint of pink (sign of deterioration) is observed. At 2-8°C, the ZYME A reagent may form a precipitate which does not affect any of the properties of the reagent and which may be redissolved by gently heating.

USE OF THE REAGENTS Allow reagents to come to room temperature (20-30°C) before using. 1. ZYME A and ZYME B reagents : • Open the ampule of reagent as indicated in the paragraph "Warnings and Precautions" (ampule with dropper-cap). • Dispense one drop of reagent. • Carefully close the bottle after use and store it as indicated in the paragraph "Storage of the reagents". 2. NIN reagent : • Open the ampule of reagent as indicated in the paragraph "Warnings and Precautions" (ampule with no dropper-cap). • Take up the contents of the ampule using a completely dry pipette and transfer this liquid into the dropper-bottle. • Fit the dropper to the bottle. • Dispense one drop of reagent. • Carefully close the bottle after use and store it as indicated in the paragraph "Storage of the reagents". 3. VP2: • Open the ampule of reagent as indicated in the paragraph Warnings and Precautions" (ampule with dropper cap). • Dispense one drop of reagent. • Carefully close the bottle after use an store it as indicated in the paragraph "Storage of the reagents".

4. Alpha-naphthol : • • • •

Reconstitute with 29 ml of 95% ethanol. Carefully close the bottle. Shake. Reagent can be used after active ingredient is completely disolved. • Dispense one drop of reagent. • Carefully close the bottle after use an store it as indicated in the paragraph "Storage of the reagents".

WARNINGS AND PRECAUTIONS • For in vitro diagnostic use only. • Qualified laboratory personnel should use aseptic technique and established precautions for infectious agents. • Do not pipette specimens or reagents by mouth. • Do not use reagents past the expiration date. • Do not allow reagents to come into contact with skin, eyes or clothing. • Do not interchange reagents or consumables between different lot numbers. • Upon removal from refrigerator, allow reagents to come to room temperature (20-30°C) before using. • Open ampules carefully as follows : - Hold the ampule in one hand in a vertical position (white plastic cap uppermost). - Press the cap down as far as possible. - Cover the flattened part of the cap with the upper part of the thumb. - Apply thumb pressure in an outward motion to the flattened part of the cap to snap off the top of the ampule inside the cap. *For ampule with no dropper-cap : - Carefully remove the cap. *For ampule with dropper-cap : - Turn the ampule upside down and maintain it in a vertical position. - Squeeze on the cap to transfer all the reagent into the dropper-bottle. • All inoculated products should be considered infectious and handled appropriately. • All patient specimens and microbial cultures are potentially infectious and should be treated with universal precautions (NCCLS M29-A: Protection of Laboratory Workeres from Instrument Biohazards and Infectious Disease Transmitted by Blood, Body Fluids, and Tissue: Approved Guideline. 1997). • After completing test, reading and interpretation, all specimens, spills and inoculated products must be autoclaved, incinerated or immersed in a germicide prior to disposal. • Interpretation of the test results should be made by a competent microbiologist who should also take into consideration the patient history, the source of the specimen, colonial and microscopic morphology and, if necessary, the results of any other tests performed, particularly the antimicrobial susceptibility patterns. • Any changes or modifications in the procedure may affect the results.

INSTRUCTIONS FOR USE Specimens and bacterial cultures should be considered infectious and handled appropriately by trained and competent technicians. Aseptic technique and usual handling precautions for the bacterial group studied should be observed throughout this procedure ; refer to Universal Precautions (NCCLS M29-A: Protection of Laboratory Workers from Instrument Biohazards and Infectious Diseases Transmitted by Blood, Body Fluids, and Tissue : Approved Guideline. 1997). For additional handling precautions, refer to Biosafety in Microbiological and Biomedical Laboratories, HHS Publication No. (CDC) 93-8395, 3rd Edition (May 1993), or to the regulations of each country. Selection of colonies Once the microorganism to be identified has been isolated and verified to be a member of the genus Streptococcus or related genera shown in Table 3 (Gram, catalase test) : • Note the type of hemolysis on the result sheet (21st test). • Pick a well-isolated colony (Note 1) and suspend it in 0.3 ml of sterile water. Homogenize well. • Flood a Columbia sheep blood agar plate (Note 2) with this suspension (or aseptically swab the entire surface of the agar). • Incubate anaerobically for 18-24 hours at 35-37°C. NOTE 1 : Alpha-hemolytic streptococci and enterococci produce sufficiently large colonies after 24 hours of incubation. For other streptococci, it is preferable to select a colony after 48 hours of incubation. For fastidious strains (producing minute colonies after 48 hours), the following procedure is recommended : - Culture the colony in 1 ml of Schaedler broth at 35-37°C for 5 hours. - Flood a Columbia sheep blood agar plate with the entire culture. Remove any excess liquid. - Incubate anaerobically for 18 hours at 35-37°C. NOTE 2 : In the case of suspected pneumococci, it is advisable to prepare 2 plates in order to obtain sufficient growth. Preparation of the strip • Prepare an incubation box (tray and lid) and distribute about 5 ml of distilled water or demineralized water [or any water without additives or chemicals which may release gases (e.g., Cl2, CO 2, etc.)] into the honeycombed wells of the tray to create a humid atmosphere. • Record the strain reference on the elongated flap of the tray. • Remove the strip from its packaging and place it in the tray. Preparation of the inoculum • Open an ampule of Suspension Medium (2 ml) as indicated in the paragraph "Warnings and Precautions" (ampule with no dropper cap) or use any tube containing 2 ml of distilled water without additives.

• Using a swab, harvest all the culture from the previously prepared subculture plate. Make a dense suspension with a turbidity greater than 4 McFarland. Inoculation of the strip • In the first half of the strip (tests VP to ____ ADH) distribute the suspension with a sterile pipette, avoiding the formation of bubbles (tilt the strip slightly forwards) : - For the tests VP to LAP : distribute approximately 100 µl into each cupule (3 drops with a Pasteur pipette ). - For the ____ ADH test : fill the tube only. • In the second half of the strip (tests RIB GLYG ) : ___ to ______ - Open an ampule of GP Medium as indicated in the paragraph "Warnings and Precautions" (ampule with no dropper-cap) and transfer the rest of the suspension into it (appr. 0.5 ml). Mix well. - Distribute this new suspension into the tubes only. GLYG ) with • Fill the cupule of the underlined tests (ADH ____ to ______ mineral oil to form a convex meniscus. • Place the lid on the tray. • Incubate at 35-37°C for 4 hours to obtain a first reading and for 24 hours to obtain a second reading if this is required. Reading of the strip After 4 hours of incubation : • Add the reagents : - VP Test : 1 drop of each of Potassium hydroxide and VP2 or alpha-naphthol. - HIP Test : 2 drops of NIN reagent. - PYRA, _GAL, _GUR, _GAL, PAL and LAP Tests : 1 drop of each of ZYME A and ZYME B reagents. • Wait 10 minutes, then read the reactions by referring to the Interpretation of Reactions Table (Table 2). If necessary, expose the strip to a strong light (10 seconds with a 1000 W lamp) to decolorize any excess reagents in the tubes PYRA to LAP. Reincubation is necessary : - if the profile cannot be found in the API 20 STREP Analytical Profile Index - if the profile is given with the following note : IDENTIFICATION NOT VALID BEFORE 24 HOURS OF INCUBATION After 24 hours, reread the reactions ESC, ____ ADH, and RIB ___ to GLYG . Do not reread the enzymatic reactions (HIP, PYRA, ______ _GAL, _GUR, _GAL, PAL, LAP) and VP. Record the reactions on the result sheet.

Identification Identification can be obtained : • using the Analytical Profile Index : the pattern of the reactions obtained must be coded into a numerical profile. On the result sheet, the tests are separated into groups of 3 and a number 1, 2 or 4 is indicated for each. By adding the numbers corresponding to positive reactions within each group, a 7-digit profile number is obtained. To obtain information on any profile not listed in the codebook, call the Voice Response System at 800-645-7056.

5 240 550 / 5 240 770 Streptococcus mutans

NOTE : The hemolytic reaction constitutes the 21st test ; betahemolysis is considered as positive with a numerical value of 4. All other hemolytic reactions are considered as negative with a numerical value of 0. Nevertheless, this test may be of discriminant value for the identification of certain species.

• using the identification software.

QUALITY CONTROL The media, strips, and reagents are systematically quality controlled at various stages of their manufacture. For those who wish to perform their own quality control tests with the strip, it is recommended that the following stock cultures be used, to obtain the results below : Table 1 VP

HIP ESC PYRA _GAL

_GUR _ GAL

PAL LAP ADH

RIB ARA MAN SOR LAC TRE INU RAF AMD GLYG

1.

–

–

–

–

–

+

–

+

+

+

+

–

–

+

+

–

–

–

+

+

2.

+

V

+

+

+

V

+

–

V

+

+

+

+

–

+

+

+

+

+

–

3.

+

+

+

V

V

+

–

V

+

+

+

–

+

+

+

+

+

+

–

–

4.

V

V

+

V

V

–

–

–

–

–

–

–

+

–

–

+

–

+

+

–

1. Streptococcus equi ssp zooepidemicus 2. Enterococcus gallinarum

ATCC 700400 ATCC 700425

3. Streptococcus uberis 4. Aerococcus viridans

ATCC 700407 ATCC 700406

ATCC : American Type Culture Collection, 10801 University Boulevard, Manassas, VA 20110-2209, USA. • Inoculum adjusted to between 4.5 and 5.5 McF. • Profiles obtained after : - 4 hours of incubation for tests VP to LAP - 24 hours of incubation for tests ____ ADH to _____ GLYG • Strains cultured on Columbia sheep blood agar

DISPOSAL OF USED MATERIAL

LIMITATIONS

After use, all ampules, swabs, pipettes, strips and incubation boxes should be autoclaved, incinerated or immersed in a disinfectant for decontamination prior to disposal.

The API 20 STREP system is intended uniquely for the identification of those species included in the database (see Identification Table (Table 3) at the end of this package insert). It cannot be used to identify any other microorganisms or to exclude their presence.

RANGE OF EXPECTED VALUES The API 20 STREP test produces color reactions which make positive and negative identification possible. Absolute values are not obtained.

INTERPRETATION OF REACTIONS Table 2 TESTS

SUBSTRATES

REACTIONS/ENZYMES

RESULTS NEGATIVE

POSITIVE

Potassium hydroxide(1 drop) +VP2 or alpha-naphthol (1drop) / wait 10 min VP

Pyruvate

Acetoin production

Colorless

Pink-Red (3) NIN (2 drops) / wait 10 min

HIP

Hippurate

Hydrolysis

ESC

Esculin

_-glucosidase

Colorless/Pale blue

Dark blue/Violet

4 hrs.

24 hrs.

4 hrs.

24 hrs.

Colorless Pale yellow

Colorless Pale yellow Light grey

Black Grey

Black

ZYME A (1 DROP) + ZYME B (1 DROP) / 10 MIN (PYRA to LAP) (1) if necessary, decolorize with intense light PYRA

Pyrrolidonyl-2-naphthylamide

Pyrrolidonyl arylamidase

_GAL

6-Bromo-2-naphthyl _-D-galactopyranoside

Colorless or very pale orange

Orange

_-galactosidase

Colorless

Violet

_GUR

Naphthol AS-BI _-D-glucuronate

_-glucuronidase

Colorless

Blue

_GAL

2-naphthyl- _-Dgalactopyranoside

_-galactosidase

Colorless or very pale violet

Violet

PAL

2-naphthyl phosphate

Alkaline phosphatase

Colorless or very pale violet

Violet

LAP

L-leucine-2-naphthylamide

Leucine arylamidase

Colorless

Orange

ADH ____

Arginine

Arginine dihydrolase

Yellow 4 hrs.

Red 24 hrs.

4 hrs.

24 hrs.

RIB ___

Ribose

Acidification

Red

Orange/Red

Orange/Yellow

Yellow

ARA ____

L-Arabinose

Acidification

Red

Orange/Red

Orange/Yellow

Yellow

MAN ____

Mannitol

Acidification

Red

Orange/Red

Orange/Yellow

Yellow

SOR ____

Sorbitol

Acidification

Red

Orange/Red

Orange/Yellow

Yellow

LAC ____

Lactose

Acidification

Red

Orange/Red

Orange/Yellow

Yellow

TRE ____

Trehalose

Acidification

Red

Orange/Red

Orange/Yellow

Yellow

INU ___

Inulin

Acidification

Red

Orange/Red

Orange/Yellow

Yellow

RAF ____

Raffinose

Acidification

Red

Orange/Red

Orange/Yellow

Yellow

Red

Orange/Red

Orange/Yellow

Yellow

AMD ____

Starch (2)

Acidification

GLYG _____

Glycogen

Acidification

Red or Orange

Bright yellow

(1) During a second reading after 24 hours of incubation, a deposit may be noticed in the tubes where the ZYME A and ZYME B reagents have been added. This phenomenon is normal and should not be taken into consideration. (2) The acidification of starch is frequently weaker than that of other sugars. (3) A pale pink color obtained after 10 minutes should be considered negative.

0

api 20 Strep

07625 B - 08/97 RECOMMENDED METHODOLOGY - Cocci - Gram + - Catalase −

Blood agar

Columbia blood agar

24:00

02

37°C

>

4 McF

Suspension Medium 2 ml

VP

ADH

ADH

GP Medium

4:00

24:00

RIB

GLYG

RIB

GLYG

37°C

37°C

VP HIP api 20 STREP

+ - + - + -

PYRA

: Potassium hydroxide + VP2 or alpha naphthol : NIN LAP : ZYME A + ZYME B

IDENTIFICATION TABLE Table 3 % of positive reactions after 4/24 hrs. at 35-37°C API 20 STREP

V6.0

VP

HIP ESC PYRA AGAL BGUR

Aerococcus viridans 1

13

50

96

54

33

16

BGAL PAL LAP ADH 37

Aerococcus viridans 2

15

70

50

76

10

20

Aerococcus viridans 3

22

88

99

40

85

48

RIB ARA MAN

SOR

LAC TRE INU RAF AMD GLYG HEM

1

5

1

83

33

85

70

83

99

25

1

5

5

25

1

35

2

70

89

14

14

1

1

8

2

82

5

91

99

33

41

70

33

1

1

5

24

1

5

37

99

14

1

1

0

25

0

100

0

3

100

1

90

0

0

0

0

0

0

20

0

0

0

0

0

Enterococcus avium

99

60

99

94

15

0

24

1

99

0

99

40

100

95

95

99

1

40

15

0

1

Enterococcus durans

100

43

100

97

32

2

76

0

91

100

99

15

2

0

84

76

0

0

56

0

18

Enterococcus faecalis

99

46

99

97

1

0

21

4

99

94

98

0

98

92

92

100

0

0

96

2

1

Enterococcus faecium

94

43

99

95

42

1

90

3

97

93

85

70

78

18

84

98

26

9

73

3

1

Enterococcus gallinarum

99

99

100

100

95

45

99

0

99

99

99

100

99

1

100

100

99

99

83

20

0

Gardnerella vaginalis

0

95

0

0

0

1

53

0

99

0

46

6

1

0

1

0

0

0

73

53

0

Gemella haemolysans

25

0

0

70

0

0

1

84

40

1

1

0

20

10

5

2

0

0

10

5

1

Alloiococcus otitis

3

0

0

35

0

0

10

35

86

4

5

0

1

0

1

11

3

1

16

5

0

Lactococcus lactis ssp cremoris

98

15

41

1

13

0

41

4

89

0

27

0

17

0

96

30

0

20

25

0

0

Lactococcus lactis ssp lactis

90

40

99

35

3

0

35

3

96

95

95

15

45

1

72

87

4

5

90

3

1

Leuconostoc spp

91

1

60

5

55

0

65

2

70

10

37

35

29

4

35

65

0

42

11

0

0

Listeria spp

Gemella morbillorum

97

79

98

0

0

0

0

0

85

0

6

0

0

0

49

92

1

1

72

0

26

Abiotrophia adiacens

0

0

10

80

0

25

0

0

99

0

0

0

0

0

0

0

0

0

0

0

0

Abiotrophia defectiva

25

0

15

99

100

0

100

0

92

0

0

0

0

0

99

100

5

93

99

0

0

1

95

4

13

1

66

30

60

96

18

17

0

42

10

70

65

0

0

10

0

0

Streptococcus agalactiae

100

99

1

1

4

79

1

96

99

99

98

0

1

1

50

87

0

1

35

4

75

Streptococcus anginosus

100

0

100

0

44

0

1

99

100

100

0

0

33

0

99

88

0

44

97

0

37

Streptococcus bovis I

97

2

100

2

71

4

14

0

97

0

2

13

86

0

100

90

63

90

100

90

0

Streptococcus bovis II 1

95

4

97

1

86

1

17

0

100

1

0

14

0

0

93

30

61

99

73

65

0

Streptococcus bovis II 2

86

4

100

13

85

88

94

0

100

13

0

1

0

0

99

99

13

72

40

13

0

0

1

25

4

95

1

80

100

100

100

100

0

0

0

99

1

0

1

99

0

100

Streptococcus acidominimus

Streptococcus canis

100

1

27

0

0

0

5

99

100

100

0

0

0

0

10

72

0

0

12

0

61

Streptococcus dys.ssp dysgalactiae

0

0

1

1

1

99

0

100

99

100

99

0

1

50

86

100

0

1

99

30

2

Streptococcus dys.ssp equisimilis

0

1

25

1

1

99

1

99

100

97

97

1

1

1

45

99

0

1

98

40

94

Streptococcus equi ssp equi

1

0

1

0

0

100

0

100

100

100

0

0

0

0

0

1

0

0

100

100

100 99

Streptococcus constellatus

0

1

15

0

0

100

1

99

100

99

85

0

0

99

100

0

0

0

99

99

Streptococcus equinus

100

0

95

0

28

0

1

1

100

0

0

0

30

0

25

7

25

15

17

10

0

Streptococcus group L

0

75

1

0

0

100

1

100

100

100

100

0

0

0

75

100

0

0

100

98

94

100

0

87

0

0

0

44

99

100

100

0

0

0

0

99

99

3

3

99

0

40

Streptococcus equi ssp zooepidemicus

Streptococcus intermedius Streptococcus mitis 1

1

0

3

1

21

0

25

35

99

19

14

1

0

1

94

7

3

26

67

5

0

Streptococcus mitis 2

0

0

3

0

31

0

35

50

100

99

1

0

1

0

100

1

1

31

84

0

0

Streptococcus mutans

99

0

99

1

64

0

1

1

100

18

0

0

99

90

90

100

81

81

1

0

1

Streptococcus oralis

0

0

1

1

50

0

46

72

100

5

1

0

1

0

99

32

1

72

96

0

0

Streptococcus pneumoniae Streptococcus porcinus

0

0

39

60

70

3

79

3

100

57

3

0

0

0

99

98

64

87

84

10

1

100

5

99

1

19

99

1

97

97

100

98

0

88

88

83

99

0

0

50

0

100

0

1

5

98

0

15

0

100

100

99

0

0

8

1

99

98

0

1

61

22

98

85

0

98

1

8

0

70

20

100

0

0

0

5

1

86

67

34

88

74

1

1

Streptococcus sanguis

0

1

42

0

63

0

1

5

100

90

0

0

1

48

83

98

33

55

67

0

0

Streptococcus suis I

0

1

82

53

80

94

76

1

100

91

0

0

7

0

94

100

75

0

100

89

0

Streptococcus pyogenes Streptococcus salivarius ssp salivarius

Streptococcus suis II

0

1

70

41

91

91

52

3

100

95

0

0

3

1

99

98

63

93

99

96

2

Streptococcus uberis

99

98

100

35

10

86

5

30

100

98

99

0

99

98

99

100

89

10

50

20

0

BIBLIOGRAPHY 1. APPELBAUM P.C., CHAURUSHIYA P.S., JACOBS M.R., DUFFETT A. Evaluation of the Rapid Strep System for Species Identification of Streptococci. (1984) J. Clin. Microbiol., 19, 588-591. 2. BALL L.C., COLMAN G. A Comparison of Conventional Methods and API Galleries for the Identification of Streptococci. (1982) International Meeting on Streptococci and Streptococcal Diseases, LUND SWEDEN, 41-42. 3. BANNISTER M.F., BENSON C.E. and SWEENEY C.R. Rapid Species Identification of Group C Streptococci Isolated from Horses. (1985) J. Clin. Microbiol., 21, 524-526. 4. COLMAN G., BALL L.C. Identification of Streptococci in a Medical Laboratory. (1984) J. Appl. Bact., 57, 1-14. 5. FACKLAM R.R., RHODEN D.L., SMITH P.B. Evaluation of the Rapid Strep System for the Identification of Clinical Isolates of Streptococcus Species. (1984) J. Clin. Microbiol., 20, 894-898.

6. HUMAN R.P. and TILLOTSON G.S. Identification of Gardnerella vaginalis with the API 20 Strep System. (1985) J. Clin. Microbiol., 21, 985-986. 7. KLOOSTERMAN R.E., CULLEN K.D. Comparison of Two Commercial Systems for the Rapid Identification of Streptococci. (1984) ASM ST. LOUIS C198. 8. MacGOWAN A.P., MARSHALL R.J., REEVES D.S. Evaluation of API 20 STREP System for Identifying Listeria Species. (1989) J. Clin. Path., 42, 548-550. 9. RUOFF K.L., KUNZ L.J. Use of the Rapid STREP System for Identification of Viridans Streptococcal Species. (1983) J. Clin. Microbiol., 18, 1138-1140. 10. TILLOTSON G.S. An Evaluation of the API 20 Strep System. (1982) J. Clin. Path., 468-471.

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